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Addgene

pSico PGK GFP
(Plasmid #12093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSico PGK GFP
  • Backbone size (bp) 7500
  • Modifications to backbone
    EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating shRNA expression.
  • Vector type
    Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSico PGK GFP is similar to pSico, except the CMV promoter is replaced with the PGK promoter. pSico PGK GFP allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/jacks-lab/protocols_table.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSico PGK GFP was a gift from Tyler Jacks (Addgene plasmid # 12093 ; http://n2t.net/addgene:12093 ; RRID:Addgene_12093)

  • For your References section:

    Cre-lox-regulated conditional RNA interference from transgenes. Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T. Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. 10.1073/pnas.0403954101 PubMed 15240889
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