pcDNA3.1_PA-Cre-Y324F
(Plasmid
#122961)
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PurposeCatalytically inactive mutant of PA-Cre
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5354
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Vector typeMammalian Expression, Cre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepcDNA3.1_PA-Cre-Y324F
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SpeciesSynthetic
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Insert Size (bp)2049
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer T7 forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_PA-Cre-Y324F was a gift from Moritoshi Sato (Addgene plasmid # 122961 ; http://n2t.net/addgene:122961 ; RRID:Addgene_122961) -
For your References section:
A photoactivatable Cre-loxP recombination system for optogenetic genome engineering. Kawano F, Okazaki R, Yazawa M, Sato M. Nat Chem Biol. 2016 Dec;12(12):1059-1064. doi: 10.1038/nchembio.2205. Epub 2016 Oct 10. 10.1038/nchembio.2205 PubMed 27723747
