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Addgene

p260 pCMV-CRE-M-AR(wt)
(Plasmid #12494)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12494 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Cre-M (BglII)
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRE-M AR
  • Alt name
    androgen receptor
  • Species
    H. sapiens (human)
  • Mutation
    CRE-M and AR ligand binding domain
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (unknown if destroyed)
  • 3′ cloning site See map (unknown if destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To generate a fusion
gene consisting of cre recombinase and the AR LBD, plasmid
p258 was digested with BglII and MluI and the hAR fragment coding for LBD was isolated. This fragment was cloned into
pCMV-CRE-M(BglII) by digesting two separate aliquots of
pCMV-CRE-M(BglII) with ScaI/BglII or with ScaI/MluI. The
ligation of these three fragments resulted in the generation of
pCMV-CRE-M-AR(wt).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p260 pCMV-CRE-M-AR(wt) was a gift from Jeffrey Green (Addgene plasmid # 12494 ; http://n2t.net/addgene:12494 ; RRID:Addgene_12494)

  • For your References section:

    Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Kaczmarczyk SJ, Green JE. Nucleic Acids Res. 2003 Aug 1. 31(15):e86. 10.1093/nar/gng087 PubMed 12888538
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