pCAGGS-mTagBFP2-T2A-iCre-ERT2
(Plasmid
#128168)
-
PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCAGGS
-
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)DH10B
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemTagBFP2-T2A-iCreERT2
-
SpeciesSynthetic
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made bypCAGGS is from BCCM/LMBP. mTagBFP2 is from Michael Davidson. iCre is from Rolf Sprengel. ERT2 is from Pierre Chambon.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor recommends the updated versions of this plasmid, PB CAG-sfGFP-T2A-iCreERT2 (https://www.addgene.org/177309/) or PB CAG-mTagBFP2-T2A-iCreERT2 (https://www.addgene.org/177308/)
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAGGS-mTagBFP2-T2A-iCre-ERT2 was a gift from Mario Capecchi (Addgene plasmid # 128168 ; http://n2t.net/addgene:128168 ; RRID:Addgene_128168) -
For your References section:
Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life. Nam HS, Capecchi MR. Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5. 10.1186/s13064-020-00139-5 PubMed 32183906
