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Addgene

Ai9
(Plasmid #22799)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22799 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Rosa26 Targeting Vector
  • Backbone size w/o insert (bp) 9862
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Best grown in bacterial strain that minimizes recombination (i.e., STBL2).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAG-Floxed tdTomato
  • Species
    n/a
  • Insert Size (bp)
    7057

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Backbone - Liqun Luo; tdTomato - Roger Tsien
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ai9 was a gift from Hongkui Zeng (Addgene plasmid # 22799 ; http://n2t.net/addgene:22799 ; RRID:Addgene_22799)

  • For your References section:

    A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. Nat Neurosci. 2010 Jan . 13(1):133-40. 10.1038/nn.2467 PubMed 20023653
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