pCSHSP:FLP
(Plasmid
#30521)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30521 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCSGFP2
- Backbone size w/o insert (bp) 3000
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Vector typeMammalian Expression ; Flp/FRT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLP recombinase
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2500
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
driven by Xenopus laevis heat shock 70 (hsp 70) promoter
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSHSP:FLP was a gift from Gerhart Ryffel (Addgene plasmid # 30521 ; http://n2t.net/addgene:30521 ; RRID:Addgene_30521) -
For your References section:
Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis. Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, Senkel S, Ryffel GU, Waldner C. Transgenic Res. 2009 Aug . 18(4):595-605. 10.1007/s11248-009-9253-4 PubMed 19266305
Map uploaded by the depositor.
