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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCVhygro
- Backbone size w/o insert (bp) 7000
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre
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Insert Size (bp)1000
- Promoter 5'LTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVhygro-Cre was a gift from Kai Ge (Addgene plasmid # 34565 ; http://n2t.net/addgene:34565 ; RRID:Addgene_34565) -
For your References section:
Histone H3K27 methyltransferase Ezh2 represses Wnt genes to facilitate adipogenesis. Wang L, Jin Q, Lee JE, Su IH, Ge K. Proc Natl Acad Sci U S A. 2010 Apr 20. 107(16):7317-22. 10.1073/pnas.1000031107 PubMed 20368440
Map uploaded by the depositor.
