pT3TS-Cre
(Plasmid
#47117)
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PurposeCan be used to in vitro transcribe Cre recombinase.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47117 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepT3TS
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Backbone manufacturerAddgene plasmid 31830
- Backbone size w/o insert (bp) 3216
- Total vector size (bp) 4247
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCre
- Promoter Lac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT3TS-Cre was a gift from Stephen Ekker (Addgene plasmid # 47117 ; http://n2t.net/addgene:47117 ; RRID:Addgene_47117) -
For your References section:
In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8. 10.1038/nmeth.1606 PubMed 21552255
Map uploaded by the depositor.
