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PurposeReporter construct for lineage tracing and mosaic analysis in zebrafish.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 60877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSK
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 11860
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Vector typeZebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namehsp70l promoter
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GenBank IDGQ856364
Gene/Insert 2
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Gene/Insert nameCre
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GenBank IDGQ856364
Gene/Insert 3
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Gene/Insert namedTomato
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GenBank IDGQ856364
Gene/Insert 4
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Gene/Insert nameUAS promoter
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GenBank IDGQ856364
Gene/Insert 5
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Gene/Insert nameGal4VP16
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GenBank IDGQ856364
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAZe was a gift from Julian Lewis (Addgene plasmid # 60877 ; http://n2t.net/addgene:60877 ; RRID:Addgene_60877) -
For your References section:
MAZe: a tool for mosaic analysis of gene function in zebrafish. Collins RT, Linker C, Lewis J. Nat Methods. 2010 Mar;7(3):219-23. doi: 10.1038/nmeth.1423. Epub 2010 Feb 7. 10.1038/nmeth.1423 PubMed 20139970
Map uploaded by the depositor.
