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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDsRed1-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression, Cre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefloxed EGFP
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Alt nameloxI EGFP loxII
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Insert Size (bp)800
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EGFP-RFP* gene switch plasmid. Floxed EGFP gene switch cassette (KpnI fragment
from plasmid "p144").
*NOTE: The "RFP" in this plasmid is actually DsRed.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p212 pCMV-EGFP/RFP was a gift from Jeffrey Green (Addgene plasmid # 8389 ; http://n2t.net/addgene:8389 ; RRID:Addgene_8389) -
For your References section:
A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679
Map uploaded by the depositor.
