AAV-U6gRNA1-U6gRNA2-TnT-Cre
(Plasmid #87682)

Ordering

Backbone

• Vector backbone
  pAAV
• Backbone size w/o insert (bp) 2900
• Total vector size (bp) 5969
• Vector type
  AAV

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Growth instructions
  Check for recombination between ITRs and U6 promoters before making AAV
• Copy number
  Unknown

Gene/Insert 1

• Gene/Insert name
  gRNA1
• Insert Size (bp)
  102
• Promoter U6

Cloning Information for Gene/Insert 1

• Cloning method Restriction Enzyme
• 5′ cloning site KpnI (not destroyed)
• 3′ cloning site MluI (not destroyed)
• 5′ sequencing primer CGGCCGCACGCGCCGGTACC
• 3′ sequencing primer CAGAAGAGCTCGCTCTTCCG
(Common Sequencing Primers)

Gene/Insert 2

• Gene/Insert name
  gRNA2
• Insert Size (bp)
  102
• Promoter U6

Cloning Information for Gene/Insert 2

• Cloning method Restriction Enzyme
• 5′ cloning site MluI (not destroyed)
• 3′ cloning site none (destroyed during cloning)
• 5′ sequencing primer GTGGAATTCCACCTGCTCAG
• 3′ sequencing primer AGGGACTTCGGGCACAATCG
(Common Sequencing Primers)

Gene/Insert 3

• Gene/Insert name
  Cre
• Insert Size (bp)
  1056
• Promoter cTnT

Cloning Information for Gene/Insert 3

• Cloning method Restriction Enzyme
• 5′ cloning site NheI (not destroyed)
• 3′ cloning site HindIII (not destroyed)
• 5′ sequencing primer ACGACTCACTATAGGCTAGC
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • AAV-U6gRNA-U6gRNA-TnT-Cre.gbk
• Articles Citing this Plasmid
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms
  • UBMTA
• Industry Terms
  • Not Available to Industry