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Addgene Videos

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Browse Addgene's collection of video content. Find video protocols, tips on using Addgene's resources, and career advice. For written lab protocol material, visit our Addgene Protocols page. Please feel free to email us at [email protected] with any questions.

Protocols

(Link opens in a new window) Video Link	Description	Related Page
Making LB Agar Plates	Create plates to culture bacteria in the lab	Pouring LB Agar Plates Protocol
Streaking Bacteria on Plates	Isolate single bacterial colonies on an agar plate	Streaking Bacteria on Plates Protocol
Diagnostic Restriction Digest	Verify a plasmid using restriction enzymes	Diagnostic Restriction Digest Protocol
Storing and Handling Addgene Plasmids	What to do after you receive your plasmids from Addgene	Instructions for Storing and Handling Plasmids
Genomic Deletions with CRISPR	Generate genomic deletions in mammalian cells lines using CRISPR/Cas9	Genomic Deletions with CRISPR Protocol
Over-Agar Antibiotic Plating	Create plates to culture bacteria in the lab	Over-Agar Antibiotic Plating Protocol
AAV Purification	Purify adeno-associated virus from a preparation produced in mammalian cells	AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol
Mulitchannel Pipetting Technique	Learn how to pipette multiple samples at once using a multichannel pipette and avoid inconsistent pipetting with our simple tips
Bacterial Transformations	We walk you through a standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and increase efficiency	Bacterial Transformation Protocol
Inoculating a Liquid Bacterial Culture	How to prepare and grow bacteria in a liquid medium	Inoculating a Liquid Bacterial Culture Protocol
Getting Started with Tissue Culture	Tips and tricks for working with tissue culture in the lab.	Blog post: 10 Basic tips for mammalian cell culture
Aseptic Technique	Aseptic technique is a critical skill for success in numerous fields such as microbiology, molecular biology or cell biology. Learn some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean workspace, and maintaining sterility while working.
AAV Titration by qPCR	Use qPCR to measure the physical titer of your adeno-associated virus preparation.	AAV Titration by qPCR Using SYBR Green Technology Protocol
Agarose Gel Electrophoresis	Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization and purification.	Agarose Gel Electrophoresis Protocol
Purifying DNA from an Agarose Gel	Gel purification allows you to isolate and purify DNA fragments based on size. Following gel electrophoresis, you can cut DNA bands out of the agarose gel and purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based cloning.	Purifying DNA from an Agarose Gel Protocol

How-To Videos

(Link opens in a new window) Video Link	Description	Related Page
MTA Guide - Requests	Your guide to the MTA process when requesting samples from Addgene	Technology Transfer
How to Order	A step-by-step walkthrough of Addgene's ordering process	Ordering Page
MTA FAQs	Answers to frequently asked questions about the MTA process	Help Center
How to Deposit	Your guide to depositing plasmids with Addgene	Depositing Page

Career Videos

(Link opens in a new window) Video Link	Description
Jessica Welch, PhD	In this first installment of the Addgene Careers series, we sit down with Addgene Outreach Scientist Jessica Welch to discuss her career path, differences in research culture between the US and Australia, and advice for current graduate students considering their future careers.
Eric J. Perkins, PhD	In this installment, we sit down with Senior Scientific Project Leader Eric Perkins to discuss the various positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific community.
Jane Hannon	Our Marketing & Outreach Specialist Jane Hannon discusses her career path, current projects in the works, and all of the things she loves about working at Addgene!
Maria Soriano	Maria Soriano, from Addgene's UK office, discusses her career in science, the importance of international collaboration, and offers career advice for scientists looking to expand their international reach.
Joel McDade	Joel McDade, Addgene's Business Development Manager, discusses his transition from the Scientist Team to Business Team, his hobbies, and advice for grauated students pursuing non-academic careers.