Abstract
Agricultural residues, such as barley straw (BS), are attractive sources for the production of chemicals and fuels based on the biorefinery principle. In the present paper, BS was steam exploded at 180°C/30 min and then 90% of the cellulose and 60% of the hemicellulose were recovered in solid and liquid fractions respectively, which were used for ethanol and xylooligosaccharides (XOS) production. In the course of enzymatic hydrolysis (EH), different solid loading (SL) (10–20% w/v) and enzyme doses (15 and 30 FPU g−1 glucan) were applied to optimize the yield of glucose concentrations, while 92 g l−1 glucose was released at 20% SL and 30 FPU g−1 glucan enzyme dosage. For ethanol production, two different process configurations were compared: separate hydrolysis and fermentation (SHF) or prehydrolysis with simultaneous saccharification and fermentation (PSSF). To transform the soluble hemicellulose into xylooligomers, two glycoside hydrolases (GH) families 10 and 11 endoxylanases were used. Reaction times, enzyme dose and several combinations of enzymes were optimized to maximize the conversion into XOS. Under the pretreatment conditions indicated above, 14 g of ethanol was obtained via the PSSF approach and 11.1 g of XOS (with DP2–DP6) was obtained per 100 g of raw material.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: The authors acknowledge the financial support by the Comunidad de Madrid-CM (Spain) (Project RESTOENE-2-CM, S2013/MAE-2882).
Employment or leadership: None declared.
Honorarium: None declared.
References
Aachary, A.A., Prapulla, S.G. (2011) Xylooligosaccharides (XOS) as an emerging prebiotic: microbial synthesis, utilization, structural characterization, bioactive properties, and applications. Compr. Rev. Food Sci. Food Saf. 10:2–16.10.1111/j.1541-4337.2010.00135.xSearch in Google Scholar
Álvarez, C., González, A., Negro, M.J., Ballesteros, I., Oliva, J.M., Saéz, F. (2017) Optimized use of hemicellulose within a biorefinery for processing high value-added xylooligosaccharides. Ind. Crops Prod. 99:41–48.10.1016/j.indcrop.2017.01.034Search in Google Scholar
Alvira, P., Tomás-Pejó, E., Ballesteros, M., Negro, M.J. (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour. Technol. 101:4851–4861.10.1016/j.biortech.2009.11.093Search in Google Scholar PubMed
Alvira, P., Negro, M.J., Ballesteros, I., González, A., Ballesteros, M. (2016) Steam explosion for wheat straw pretreatment for sugars production. Bioethanol 2:66–75.10.1515/bioeth-2016-0003Search in Google Scholar
Biely, P., Singh, S., Puchart, V. (2016) Towards enzymatic breakdown of complex plant xylan structures: state of the art. Biotechnol. Adv. 34:1260–1274.10.1016/j.biotechadv.2016.09.001Search in Google Scholar PubMed
Cara, C., Ruiz, E., Carvalheiro, F., Moura, P., Ballesteros, I., Castro, E., Gírio, F. (2012) Production, purification and characterisation of oligosaccharides from olive tree pruning autohydrolysis. Ind. Crops Prod. 40:225–231.10.1016/j.indcrop.2012.03.017Search in Google Scholar
Cherubini, F. (2010) The biorefinery concept: using biomass instead of oil for producing energy and chemicals. Energ. Convers. Manage. 51:1412–1421.10.1016/j.enconman.2010.01.015Search in Google Scholar
Deloule, V., Chirat, C., Boisset, C., Toussaint, B., Chroboczck, J. (2017) Production of hemicellulose oligomers from softwood chips using autohydrolysis followed by an enzymatic post-hydrolysis. Holzforschung 71:575–581.10.1515/hf-2016-0181Search in Google Scholar
Dionex Analysis of Carbohydrates by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) (2000) Technical Note 20.Search in Google Scholar
Duque, A., Manzanares, P., Ballesteros, I., Ballesteros, M. (2016) Steam explosion as lignocellulosic biomass pretreatment. In: Biomass Fractionation Technologies for a Lignocellulosic Feedstock Based Biorefinery. Ed. Mussatto, S.I. Elsevier, Amsterdam. pp. 349–368.10.1016/B978-0-12-802323-5.00015-3Search in Google Scholar
European Environment Agency (www.eea.europa.eu/). Greenhouse gas emissions from transport (last modified 09 25 2018).Search in Google Scholar
FAOSTAT (2016) http://faostat3.fao.org/ (last accessed 4.29.2018).Search in Google Scholar
García-Aparicio, M.P., Oliva, J.M., Manzanares, P., Ballesteros, M., Ballesteros, I., González, A., Negro, M.J. (2011) Second generation ethanol production from exploded barley straw by Kluyveromyces marxianus CETC 10875. Fuel 90:1624–1630.10.1016/j.fuel.2010.10.052Search in Google Scholar
Gibson, G.R., Hutkins, R., Sanders, M.E., Prescott, S.L., Reimer, R.A., Salminen, S.J., Scott, K., Stanton, C., Swanson, K.S., Cani, P.D., Verbeke, K., Reid. G. (2017) Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat. Rev. Gastroenterol. Hepatol. 14:491–502.10.1038/nrgastro.2017.75Search in Google Scholar PubMed
Gullón, P., Gullón, B., Cardelle-Cobas, A., Alonso, J.L., Pintado, M., Gomes, A. (2014) Effects of hemicellulose-derived saccharides on behavior of Lactobacilli under simulated gastrointestinal conditions. Food Res. Int. 64:880–888.10.1016/j.foodres.2014.08.043Search in Google Scholar PubMed
Hodge, D.B., Karim, M.N., Schell, D.J., McMillan, J.D. (2008) Soluble and insoluble solids contributions to high-solids enzymatic hydrolysis of lignocellulose. Bioresour. Technol. 99:8940–8948.10.1016/j.biortech.2008.05.015Search in Google Scholar PubMed
Huang, C., Chenhuan, L., Xinxing, W., Yang, H., He, J., Huang, C., Li, X., Yong, Q. (2017) An integrated process to produce bio-ethanol and xylooligosaccharides rich in xylobiose and xylotriose from high ash content waste wheat straw. Bioresour. Technol. 241:228–235.10.1016/j.biortech.2017.05.109Search in Google Scholar PubMed
Kajaste, R. (2014) Chemicals from biomass – managing greenhouse gas emissions in biorefinery production chains – a review. J. Clean. Prod. 75:1–10.10.1016/j.jclepro.2014.03.070Search in Google Scholar
Koppram, R., Tomás-Pejó, E., Xiros, C., Olsson, L. (2014) Lignocellulosic ethanol production at high-gravity: challenges and perspectives. Trends Biotechnol. 32:46–53.10.1016/j.tibtech.2013.10.003Search in Google Scholar PubMed
Linares-Pastén, J.A., Aronsson, A., Karlsson, E.N. (2018) Structural considerations on the use of endo-xylanases for the production of prebiotic xylooligosaccharides from biomass. Curr. Protein Pept. Sci. 19:49–67.10.2174/1389203717666160923155209Search in Google Scholar PubMed PubMed Central
López-Linares, J.C., Ballesteros, I., Tourán, J., Cara, C., Castro, E., Ballesteros, M., Romero, I. (2015) Optimization of uncatalyzed steam explosion pretreatment of rapeseed straw for biofuel production. Bioresour. Technol. 190:97–105.10.1016/j.biortech.2015.04.066Search in Google Scholar PubMed
Martín-Sampedro, R., Eugenio, M.E., García, J.C., López, F., Villar, J.C., Díaz, M.J. (2012) Steam explosion and enzymatic pre-treatments as an approach to improve the enzymatic hydrolysis of Eucalyptus globulus. Biomass Bioenerg. 42:97–106.10.1016/j.biombioe.2012.03.032Search in Google Scholar
Mechelke, M., Koecka, D.E., Broekera, J., Roesslera, B., Krabichlera, F., Schwarza, W.H., Zverlova, V.V., Liebla, W. (2017) Characterization of the arabinoxylan-degrading machinery of the thermophilic bacterium Herbinix hemicellulosilytica – six new xylanases, three arabinofuranosidases and one xylosidase. J. Biotechnol. 257:122–130.10.1016/j.jbiotec.2017.04.023Search in Google Scholar PubMed
Modenbach, A.A., Nokes, S.E. (2013) Enzymatic hydrolysis of biomass at high-solids loadings – a review. Biomass Bioenerg. 56:526–544.10.1016/j.biombioe.2013.05.031Search in Google Scholar
Moncada, J., Aristizábal, V., Cardona, C.A. (2016) Design strategies for sustainable biorefineries. Biochem. Eng. J. 116:122–134.10.1016/j.bej.2016.06.009Search in Google Scholar
Morgan, N.K., Wallace, A., Bedford, M., Choct, M. (2017) Efficiency of xylanases from families 10 and 11 in production of xylo-oligosaccharides from wheat arabinoxylans. Carbohyd. Polym. 167:290–296.10.1016/j.carbpol.2017.03.063Search in Google Scholar PubMed
Muzamal, M., Jedvert, K., Theliander, H., Rasmuson, A. (2015) Structural changes in spruce wood during different steps of steam explosion pretreatment. Holzforschung 69:61–66.10.1515/hf-2013-0234Search in Google Scholar
Oliva, J., Negro, M., Manzanares, P., Ballesteros, I., Chamorro, M., Sáez, F., Ballesteros, M., Moreno, A. (2017) A sequential steam explosion and reactive extrusion pretreatment for lignocellulosic biomass conversion within a fermentation-based biorefinery perspective. Fermentation 3:15.10.3390/fermentation3020015Search in Google Scholar
Otiendo, D.O., Ahring, B.K. (2012) The potential for oligosaccharide production from the hemicellulose fraction of biomasses through pretreatment processes: xylooligosaccharides (XOS), arabinooligosaccharides (AOS), and mannooligosaccharides (MOS). Carbohydr. Res. 360:84–92.10.1016/j.carres.2012.07.017Search in Google Scholar PubMed
Patel, S., Goyal, A. (2012) The current trends and future perspectives of prebiotics research: a review. 3 Biotech 2:115–125.10.1007/s13205-012-0044-xSearch in Google Scholar
Renewable Energy Directive (2016) Proposal for a Directive of the European Parliament and the Council on the promotion of the use of energy from renewable source. COM (2016) 0767.Search in Google Scholar
Rico, X., Gullón, B., Alonso, J.L., Parajó, J.C., Yañez, R. (2018) Valorization of peanut shells: manufacture of bioactive oligosaccharides. Carbohyd. Polym. 183:21–28.10.1016/j.carbpol.2017.11.009Search in Google Scholar PubMed
Ruiz, E., Gullón, B., Moura, P., Carvalheiro, F., Eibes, G., Cara, C., Castro, E. (2017) Bifidobacterial growth stimulation by oligosaccharides generated from olive tree pruning biomass. Carbohyd. Polym. 169:149–156.10.1016/j.carbpol.2017.04.014Search in Google Scholar PubMed
Schütt, F., Puls, J., Saake, B. (2011) Optimization of steam pretreatment conditions for enzymatic hydrolysis of poplar wood. Holzforschung 65:453–459.10.1515/hf.2011.066Search in Google Scholar
Searle, S., Malins, C. (2013) Availability of cellulosic residues and wastes in the EU. International Council on Clean Transportation, White paper.Search in Google Scholar
Slavin, J. (2013) Fiber and prebiotics: mechanisms and health benefits. Nutrients 5:1417–1435.10.3390/nu5041417Search in Google Scholar PubMed PubMed Central
Sluiter, J.B., Ruiz, R.O., Scarlata, C.J., Sluiter, A.D., Templeton, D.W. (2010) Compositional analysis of lignocellulosic feedstocks. 1. Review and description of methods. J. Agric. Food Chem. 58:9043–9053.10.1021/jf1008023Search in Google Scholar PubMed PubMed Central
Van Dyk, J.S., Pletschke, B.I. (2012) A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes – factors affecting enzymes, conversion and synergy. Biotechnol. Adv. 30:1458–1480.10.1016/j.biotechadv.2012.03.002Search in Google Scholar PubMed
Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/hf-2018-0101).
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