Cannabis Grower's Handbook: The Complete Guide to Marijuana and Hemp Cultivation

CANNABIS

GROWER’S

HANDBOOK

CANNABIS GROWER’S HANDBOOK

The Complete Guide to Marijuana and Hemp Cultivation

Copyright © 2021 Ed Rosenthal

Published by Quick American Publishing

A division of Quick Trading Company

Piedmont, California, USA

Printed in Canada

First Printing

ISBN 9781936807543

Ebook ISBN 9781936807550

Project Director: Jane Klein

Project Manager and Chief Editor: Angela Bacca

Copy Editor: Paula Dragosh

Editorial Review: Joey Ereñeta, Ellen Holland, Jeff Jones

Art Director/Cover Design: Christian Petke

Cover Photo: Phil Sullivan, HRS Agriculture/Team Terpene

Interior Design: Scott Idleman, Blink

Production Editor: Christy Quinto

Photographs as noted.

Names: Rosenthal, Ed, author. | Flannery, Robert, 1978- author. | Bacca, Angela, 1986- author.

Title: Cannabis grower’s handbook : the complete guide to marijuana and hemp cultivation / Ed Rosenthal, Dr. Robert Flannery, Angela Bacca.

Description: Piedmont, California : Quick American Publishing, 2021. | Includes bibliographical references.

Identifiers: LCCN 2021021606 (print) | LCCN 2021021607 (ebook) | ISBN 9781936807543 (trade paperback) | ISBN 9781936807550 (epub)

Subjects: LCSH: Cannabis. | Marijuana. | Marijuana industry.

Classification: LCC SB295.C35 R6583 2021 (print) | LCC SB295.C35 (ebook) | DDC 633.5/3–dc23

LC record available at https://lccn.loc.gov/2021021606

LC ebook record available at https://lccn.loc.gov/2021021607

All rights reserved. No part of this book may be used or reproduced in any manner whatsoever without written permission of the Publisher, except in the case of brief quotations embodied in critical articles and reviews. If you are considering using this title as a textbook in an upcoming course, or are selecting portions of the content in this title as course materials, please contact the publisher for bulk sales and/or licensing information.

CANNABIS

GROWER’S

HANDBOOK

Ed Rosenthal

Dr. Robert Flannery and Angela Bacca

DEDICATION

This series continues to be dedicated to Pete Seeger (1919–2014).

God bless the grass that grows through the crack.

—Malvina Reynolds, God Bless the Grass

This edition of the Cannabis Grower’s Handbook is dedicated to Dennis Peron (1945–2018).

All use of marijuana is medical. —Dennis Peron

William Tell has stretched his bow til it won’t stretch no furthermore And/or may it require a change that hasn’t come before.

—St. Stephen, Robert Hunter, The Grateful Dead

ACKNOWLEDGMENTS

Dabsel Adams, AEssenseGrows, Kristen Angelo, Justin Arriola, Jeremy Babbit, Barney’s Farm, George Bianchini, Thomas Blank, PhD, Kristine Borghino, Lily Borghino, The Brand Family, Bruce Bugbee, PhD, Frenchy Cannoli, Sunshine Cereceda, Central Coast Agriculture, Julie Chiarello, Tommy Chong, Laura and Marty Clein, Remo and Sandra Colsanti, Chris Conrad, Jose Luis Cordovez, Lizzy Cozzi, Dank Duchess, Steve DeAngelo, Kymron DeCesare, Doobie Duck, David Downs, Daniel Old E and Brian Bleezy Eatmon of Mendo Dope, ECO Cannabis in Oakland, Mahmoud ElSohly, PhD, Phil Emerson, Joey Ereñeta, Nico Escondido, Bill Faulconer, Will Ferguson, Tera Flannery, Mel Frank, Laura Galli, Emery Garcia, Reginald Gaudino, PhD, Gregory Gerdeman, PhD, Dan Grace, Bill Graham, Mark Gray, Mark Grayshock, GreenSourceGardens, Grow Magazine, GrowWeedEasy.com, Ellen Holland, Jake Holley, Humboldt Seeds, Dale Hunt, PhD, Dale Sky Jones, Jeff Jones, Kandid Kush, Autumn Karcey, Brandy Keen, Caleb King, Ted Kinsman, Jay Kitchen, Wendy Kornberg, John Kozlowski, La Osa, Donald P. Land, PhD, Troy Larkin, Gopi Lennon, Heiner Lieth, PhD, Helios Lima, James Loud, Mattie Mead, Jeff Lowenfels, Brian Lundeen, Brian Malin, Neil Mattson, PhD, Max Montrose, Miranda Moore, Kristin Nevedal, Amber O’Neill, Christian Petke, Phytonyx, Professor P, Christy Quinto, Rosa Raudales, PhD, Jesse Robertson, Felisa Rogers, Mary Shapiro, Autumn Shelton, SKUNK Magazine, Brian Spivey, Clayton Stewart, StickyFields, Stinkbud, Lucas Strazzeri, Phil Sullivan aka Team Terpene, Picture Fotografie, Seth Swanson, Dr. Robert Thornton, Chris Trump, Andy Unruh, Dan Vinkovetsky (formerly known as Danny Danko), Alexander Wild, Kale Worden, Giacobazzi Yanez, Zoom_Gardens

A special thanks to our sponsors:

Advanced Nutrients, AgGas, Air Sniper, Apogee Instruments, ARBICO Organics, Bionova, Cannabis Training University, Cannatrol, Cryo Cure, Dyna-Gro, Dynasty Genetics, Earth Witch Seeds, Ecological Laboratories Inc., EYE Hortilux, Fresh Headies, Garden City Fungi, Genesys Global, GreenBroz Inc., Green Goddess Supply, Green House Seed Co., Greenshock Farms, Grobo, Grodan, Groundwork BioAg, Grow Glide, Growlink, Gutenberg’s Dank Pressing Co., Hanna Instruments, Happy Tree Microbes, Harvest More, Hibred Seed, Horticulture Lighting Group, Lost Coast Plant Therapy, Humboldt Seed Company, Humidicorp, iHort, microBIOMETER, Microclone Tissue Culture, Miicrobial Mass, Mondi Products, Munch Machine, Neptune’s Organics, Oaksterdam University, Paradise Seeds, Pinch and Pull, Plant Success Organics, Power Grown, Pulse Grow, Pure Pressure, Purple Caper Seeds, Reiziger Holland, Rocket Seeds, Royal Queen Seeds, Sasquatch Soil Company, Scynce LED, Sensational Solutions, Sensi Seeds, Smart Bee Controllers, Smart Pots, Spray-N-Grow, STM Canna, Suite Leaf, Surna, Taoshops S.R.O., The Seed Cellar, TNB Naturals, Tom’s Tumble Trimmer, True Liberty Bags, Vital Garden Supply, Weed Guardians Family Tree

TABLE OF CONTENTS

Preface

Foreword

The Cannabis User’s Bill of Rights

Introduction

PART I: THE CANNABIS PLANT

Understanding the Effects of Cannabis

The Cannabis Plant

Terpenes

Indica, Sativa, Ruderalis & Hybrids

Autoflowering Varieties

Choosing Plants to Grow

Variety Showcase

Basic Breeding

PART II: THE LIMITING FACTORS

The Cannabis Plant Life Cycle

Photosynthesis

Light

Carbon Dioxide

Water

Oxygen

Nutrients & Fertilizers

Air Temperature, Humidity & Qualities

PART III: SETTING UP THE GARDEN

Goals

Soil

Hydroponics

Vertical Gardens

Electricity Basics & Load Calculation

Designing Personal Spaces

Designing Medium & Large Spaces

Featured Gardens & Growing Styles

Dennis Peron’s Revolutionary Balcony Garden

KISS Garden

Big Floating Buds: Tyler’s Aquaponic Garden

Oklahoma Sungrown: Localized Breeding with Fungi & Hügelkultur

Creswell Oreganics Octagon Garden

Autumn Brands Dutch-Style Flower Farming

The California Appellation of Origin Garden

Planting by the Moon: The Martyjuana™ Garden

Dry Farming with Sunboldt Grown

Growing Giant Terpy Emerald Triangle Plants with Mendo Dope & Greenshock Farms

Remo’s High-Tech, High-Yield & High-Efficiency Indoor Garden

Korean Natural Farming

Sustainability

PART IV: THE PLANT LIFE CYCLE

Getting Started

Vegetative Growth

Flowering

Finishing & Flushing

PART V: HARVESTING & PROCESSING

When & How to Harvest

Picking

Trimming

Automation

Drying, Curing & Storing

Processing

APPENDIX A: Cloning

APPENDIX B: Tissue Culture

APPENDIX C: Producing Seeds

APPENDIX D: Regeneration

APPENDIX E: Pest & Disease

Glossary

Bibliography

Garden Consultants

PREFACE

by Steve DeAngelo

Ed Rosenthal and I emerged from the same underground cauldron of culture and politics known as the Youth International Party, more commonly referred to as the Yippies or sometimes even just Yippie! (The exclamation point was very important to us.) Although the Yippies were often written off as the court jesters of the New Left movement of the ’60s and ’70s, the Yippie! movement gave birth (or at least shelter) to a cadre of remarkably effective and absolutely determined cannabis activists who would go on to play crucial roles in the formation of the modern cannabis movement and industry.

Steve DeAngelo speaking at the kickoff of the Hemp Tour at Penn State’s Earth Day, circa 1987. Courtesy of Steve DeAngelo

Like old CIA officers, old Yippies never really retire. Ed and I are both fortunate to have now reached elder status, but in the early 1970s I was just about the youngest Yippie and Ed had been around the scene a lot longer; he was this impressive, distant figure I heard about long before we met. I think I first met him at the 1975 White House Smoke-In, though it could have been the 1977 White House Smoke-In, or possibly while smoking or even selling weed in a smoky commune somewhere.

Marijuana Grower’s Guide, the original edition of this book, seems to have always been present in my life, and I recall my reaction upon first seeing it; it was one of the most powerfully subversive books ever written. Here was an extremely specific, detailed, impeccably researched and written guide to producing the best cannabis in the world. The only other instructions for growing cannabis that I had seen had been mimeographed handouts passed from hippie to hippie, or the rudimentary drawings sometimes published in underground newspapers. I could tell right away that this book was going to change our world.

At the time, almost all the cannabis consumed in the United States was imported from countries like Mexico, Jamaica, and Lebanon. Ed had produced a tool that would allow any reasonably dedicated cannabis consumer to liberate themselves from this very long, expensive supply chain and, with a steady application of effort, might also enable them to grow enough for their friends and family.

Marijuana Grower’s Guide taught the earliest domestic growers how to produce higher-quality cannabis than what was coming from other countries. Appreciation for imported cannabis began to wane. More people began to consume it as higher quality became more available, and the earliest cannabis breeders built on the foundation Ed had laid. In a stroke of good fortune (or divine intervention), this high-quality cannabis was abundant in California during the AIDS crisis, where it played a key role in preserving and enhancing the quality of life of AIDS patients. This real-life demonstration of the therapeutic properties of cannabis convinced the voters of California, and eventually other states and nations, to pass cannabis initiatives.

Today the millions of people who have been introduced to cannabis since the first publication of Marijuana Grower’s Guide have coalesced into a powerful movement that will ensure that everybody who needs cannabis has safe and affordable access to it and that one day the prison doors are thrown open and every single cannabis prisoner on Planet Earth comes home to their family—with the resources they need to rebuild the lives that were stolen from them.

Thank you, Ed, for all you have done to teach and guide us on our common mission. Always Onward, Forever Free!

Steve DeAngelo in Canada, 2019. Photo: Giacobazzi Yanez

FOREWORD

by Tommy Chong

Well, here we go again. Ed has written another book. Just as before, all my close friends are still potheads, and Ed is still not only a pothead, he is the pot-growing expert who holds the distinction of turning more people on to pot than Cheech and Chong.

Photo: Kerry Reynolds

What has changed since the last foreword I wrote is that he is not worrying so much about the DEA chasing down the hundreds of thousands of growers currently tending gardens all over America because, hey man, it’s getting legal everywhere!

I admire and respect Ed because he is a great revolutionary and anti establishment hippie who likes to wear weird clothes while he outs corrupt federal officials who can no longer freely enforce America’s sadly outdated drug laws. In 2003, Ed faced 20 years in federal prison for doing pretty much what he had been doing for the past 30 or so years—growing pot and teaching others to grow their own. The feds insisted Ed broke the law, and Ed would agree with them and then explain, It’s a terrible law!

Another reason why I admire and respect this man is that writing is hard, comedy is easy. Ed’s books have been every grower’s go-to textbooks when it comes to growing good pot and combating disease, thieves, and other pests that could waste an entire growing cycle. He hasn’t just taught people to grow, he is one of many advocates who fought to bring about the dearly needed changes in the law so that pot growers are treated much like any other farmer in America.

My memory is good, my brain is so good, and it is thanks to weed. It is all about weed. Our bodies are like computers. If you know what button or what app to hit, you can learn everything there is to know. And, if you know how to get the energy source, you know how to recharge the batteries.

Ed has the whole field covered. Ask Ed. I do on occasion still ask Ed. Ed is probably one of the most intelligent men I know, and he does have the answer to quite a few problems facing Americans today. So, if you have the time and the inclination, read what he has to say. Things are changing for the better, and even the United States government has backed off when it comes to arguing with Ed. I have to say that as the pot laws are changing and, one by one, begin to disappear, we can take a moment before we light up that now legal joint and say a silent, Thanks, Ed Rosenthal, because he stood in front of the government tank and faced it down … and he was totally zonked out of his mind when he did it.

Thank you, Ed.  —Tommy Chong

THE CANNABIS USER’S BILL OF RIGHTS

Adults shall not be prohibited from using or possessing cannabis.

All adults, medical patients, and caregivers of medical patients shall have the right to grow their own cannabis for noncommercial use.

Laws regulating commercial cannabis markets should be fair, open, regulated for health and consumer protection, and allow easy access for buyers and sellers.

A medical use designation shall be determined by patients in consultation with their doctor without interference from the government.

Cannabis users shall not be discriminated against by employers and landlords.

Cannabis use shall not be considered when determining child custody.

There shall be no taxes or fees on personal use cultivation.

Photo: La Osa

THE TOMATO MODEL

The model for what cannabis legalization should look like is already out there. It’s tomatoes. More tomatoes are grown in America by home gardeners than are produced commercially, yet there is a robust commercial market for tomatoes and tomato products of all types: canned, vine ripened, organic, sauces, soups, ketchup, and so on. At the same time, small-scale specialty cultivators do well selling their produce at farmers’ markets, and home gardeners with extra tomatoes share the bounty with neighbors as gifts, in trade, or through informal sales. Cannabis should be handled in the same way. Commercial growers can thrive side by side with home and specialty cultivators.

INTRODUCTION

by Angela Bacca

After legalization, elementary botany classes could use this guide as a casebook for learning about a common plant.

—Raymond A. Sokolov, New York Times book review of Marijuana Grower’s Guide, by Mel Frank and Ed Rosenthal, April 16, 1978

The earliest iterations of this book were a call to action: Overgrow the Government.

In 1971, President Richard Nixon formally declared the War on Drugs, and in 1973 he created the Drug Enforcement Administration (DEA), one of several agencies that would receive ever-increasing federal funding for the purpose of eradicating illegal drugs and the illegal drug trade. Of the many weapons used in the war against marijuana production and use, the US government sprayed both domestic and Mexican-grown crops with Paraquat, a deadly pesticide.

In 1978, the Washington Post summed up the policy in a column titled Marijuana Outrage. The irony is simply this: the government that failed over many decades to convince the public that marijuana was dangerous finally has helped make it dangerous.

When Marijuana Grower’s Guide by Ed Rosenthal and Mel Frank was published in 1974, it was the first-of-its-kind gardening guide about the cannabis plant since federal prohibition began with the Marihuana Tax Act of 1937. It was more than a DIY gardening book; it was a guide to active protest.

In turn, the American grow-your-own movement flourished in the late 1970s and early 1980s. As the preface to the 1984 edition of Marijuana Grower’s Handbook by Ed Rosenthal put it, The more the government stepped up its eradication attempts aimed at imports, the more mini-gardens and mini-farms began to develop in the U.S. In simple-to-understand language, Marijuana Grower’s Guide made experts out of hardening hobbyists.

Generations of growers around the world would learn to grow cannabis by reading and sharing Ed’s early books, articles, and Ask Ed columns, which originated with the founding of High Times Magazine in 1974 to answer readers’ cannabis questions. Nearly 50 years later, new and experienced cultivators around the world are still reading his columns in their local cannabis publications and sending Ed their questions, photos, and observations from their unique home gardens.

Cannabis grower-researchers inspired by these early writings fueled the global innovation and study of indoor agriculture. Through artificial lighting, climate controls, and hydroponics, not only did indoor gardeners evade law enforcement, but they provided the controls necessary to experiment, breed, and innovate.

In the late 1980s and early 1990s, Ed was a prominent advocate in the creation of the nation’s first medical cannabis laws, first with the passage of Proposition P in the City of San Francisco in 1991 and next as a part of a broader coalition to support the passage of California’s Proposition 215 in 1996, the first state-level medical cannabis law in the United States. Both measures were championed by the late Dennis Peron, to whom we have dedicated this edition of the Grower’s Handbook.

This early regulation-free legislation in California led to the proliferation of cannabis gardens, varieties, innovations in cultivation practices, by-products, and methods of ingestion. It also solidified California as the epicenter of the burgeoning global industry. The Marijuana Grower’s Handbook was updated in 1998 to include new technologies, as more and more growers found their way to the cannabis plant and indoor cultivation.

In the early 2000s, the first city-sanctioned dispensaries and grow operations appeared in the San Francisco Bay Area, and Ed was deputized by the City of Oakland to oversee the large-scale production of starter plants (clones) to supply state legal growers through these storefronts.

In 2002, the DEA carried out several predawn raids on San Francisco dispensaries and Ed’s Oakland home publishing office, where he was arrested and put on trial as the kingpin of a major illegal drug operation. The judge barred Ed’s defense from testifying that he had been deputized by the city or that the operation was in compliance with local ordinances and state law.

Many of the original members of the Youth International Party, aka The Yippies, went on to become pivotal figures in the cannabis legalization movement and industry, including Ed Rosenthal, Steve DeAngelo, and Dana Beal. The Yippies were memorialized in the 2020 Netflix movie The Trial of the Chicago 7.

Using the Yippie tactic of drawing attention to injustice through spectacle, Ed attended his federal trial in a Wizard of Weed costume. The international media attention this garnered helped swell the crowds of protestors outside the federal courthouse.

They had to lie in order to win, so who was actually on trial? Ed asked. "By the time it got to The New York Times, the law was on trial."

The jury was forced to convict Ed on the evidence presented, but many jurors publicly denounced their verdict when they learned the government had barred them from hearing the truth. The public backlash from the political prosecution and public statements from jurors largely factored into Ed’s June 2003 sentencing to one day in federal prison with credit for time served in a local jail the day of the arrest.

This is day one in the crusade to bring down the marijuana laws. The federal government makes no distinction between medical and recreational marijuana. They’re right, all marijuana should be legal, Ed said in a press conference after the sentencing, vowing to fight his conviction despite the lenient sentencing. The appeals would continue unsuccessfully until 2007.

I started working for Ed in the summer of 2008 at the end of the George W. Bush era, which was characterized by the sort of political prosecutions against state-legal cannabis that Ed endured. My first project was a revised edition of Marijuana Grower’s Handbook. Early on in the production of the book the newly inaugurated Obama administration signaled a sea change in federal priorities regarding cannabis. The so-called Ogden Memo stated the new administration’s look-the-other-way policy on state medical cannabis laws.

A flood of growers and aspiring cannabis entrepreneurs made their way to Oakland to attend classes at Oaksterdam University and learn from Bay Area pioneers in the business, including Ed and Dennis Peron. Oaksterdam’s founder, Richard Lee, put up over $1 million of his own money to place a legalization initiative (Proposition 19) on the California ballot in 2010. While we went to print that year truly believing cannabis would be legal within months, the narrow defeat of Proposition 19 paved the way for the successful passage two years later of the first adult-use legalization laws in Colorado and Washington state.

While the previous edition of this book ushered in the Green Rush, this edition comes at a time when marijuana, now exclusively referred to as cannabis, has mainstreamed. Real and meaningful research is being conducted on cannabis cultivation, genomics, medicine, processing, and use. Most people who will pick up this book can grow legally, either for personal or for medical use or as a licensed commercial grower. More and more schools offer courses on cannabis cultivation.

This book is for everyone: the home grower, those looking to get into the commercial industry, and especially for seasoned educated growers who wish to continue to learn and expand their techniques and knowledge. It’s designed to be a useful educational tool that breaks down general concepts and explains and applies them for both home and commercial gardens. While some may choose to read it cover to cover, others will use it as a staple reference in the garden, for the answers to any and all of their questions lie within these pages.

Last time we knew the contributions and reviews from a handful of credentialed scholars, academics, and researchers added to the legitimacy and respect of the book. Still, most contributed their writing under pseudonyms or (as requested) reviewed content without recognition. This time, we are particularly proud of the accomplished contributors who have made this edition the new groundbreaking standard for all cannabis grow books to come and who can finally claim their bylines.

This edition adds two coauthors: Dr. Robert Flannery of Dr. Robb Farms, and the author of this introduction, Angela Bacca.

Dr. Robert Flannery is the first PhD in the United States with technical training and expertise in commercial cannabis cultivation. He has a PhD in plant biology with an emphasis in environmental horticulture and a specific expertise in hydroponic crop optimization for cut-flower production from the University of California at Davis. Dr. Flannery has managed commercial horticultural cultivation since 1999. While working on his doctorate, he began advising cultivators throughout Northern California on modern horticulture practices.

I have been editing and writing cannabis-specific content ever since I was a student journalist at San Francisco State University in 2006. I edited and managed the 2010 edition of Grower’s Handbook while working on an MBA at Mills College and (with Ed) supporting the Proposition 19 campaign in any way I could. Since that time I have edited several books and magazines in the field and specialized in long-form journalistic coverage of the economic and political rollout of commercial cannabis markets in different states from a business, horticulture, economics, and ethics perspective.

In addition to contributions from Dr. Robb and me, what makes this edition so special are all the outside contributors and editors who have ensured that Cannabis Grower’s Handbook is the most thorough and comprehensive cannabis cultivation guide available.

Part I: The Cannabis Plant describes how cannabis produces its range of effects in humans, outlines the plant’s taxonomy and history, and breaks down cannabis varieties and basic breeding.

In the previous edition of the book, one of the nation’s leading cannabis researchers, under the pseudonym G. Lee PhD, wrote about how cannabis works with human physiology. In this edition, Dr. Gregory Gerdeman is able to formally claim his byline and update the content to reflect the advances of scientific understanding over the last decade. Dr. Gerdeman is a neuroscientist and educator who has the distinction of studying the endocannabinoid system (ECS) for nearly three decades. His graduate research included some of the world’s first discoveries that endocannabinoids act as retrograde messengers that regulate synaptic plasticity in the brain, findings that have become a foundation to modern understanding of the ECS and the neuroprotective actions of cannabinoids.

Corrections to common misconceptions about female cannabis flower anatomy were provided by Mel Frank, the co-author of the original Marijuana Grower’s Guide (1974).

In the review of cannabis varieties, we have included an expanded feature on autoflowering plants by Jeff Lowenfels, a reformed lawyer and author of Teaming with Microbes and the whole Teaming With book series. These quick-harvesting varieties have emerged as the most intriguing new cultivars in many commercial markets and home gardens, and cannabis breeders are producing more and more of them.

Our terpene section has been expanded to include new research and newly identified cannabis terpenes. This content was revised by the team at Royal Queen Seeds and Ellen Holland, a longtime cannabis journalist and editor who contributed greatly to the production of this book.

This edition also includes a chapter on basic breeding by Professor P of Dynasty Genetics and Will Ferguson. Supplemental information about genomic testing, breeding, and cannabis intellectual property was provided by Dale Hunt, JD, PhD, of Plant & Planet Law Firm and Breeder’s Best. Content about producing diploid and triploid seeds was provided by Emery Garcia of Oregon CBD.

Part II: The Limiting Factors explains the key inputs that drive the process of photosynthesis and therefore plant growth: light, carbon dioxide, water, oxygen, nutrients and fertilizers, and air temperature, humidity and qualities. Each chapter breaks down basic concepts, techniques, products, and how to apply this information to the garden.

The Light chapter was revised and expanded by Jake Holley, who at the time of first printing is earning his PhD in horticultural biology with a specialty in plant lighting at Cornell University, where he conducts research. This chapter received further technical review by Bruce Bugbee, PhD, professor at Utah State University and founder of Apogee Instruments. The Water chapter was revised and edited by Rosa Raudales, PhD, a professor at the University of Connecticut. The Nutrients & Fertilizers chapter was revised and edited by Neil Mattson, PhD, professor at Cornell University. The Air Temperature, Humidity & Qualities chapter was revised by the authors with contributions from Oaksterdam University horticulture professor Joey Ereñeta, Autumn Karcey of Cultivo, Inc., and Brandy Keen.

Part III: Setting Up the Garden covers choosing a plant growth medium and/or nutrient-delivery system as well as the basic elements of indoor and outdoor garden design for personal and commercial operations. Because there is no one right way to grow, we included a showcase of garden profiles that cover a range of styles, from fully organic regenerative outdoor gardening techniques to high-efficiency indoor gardens.

The Soil chapter was revised by Brian Malin of Vital Garden Supply, with supplemental information about regenerative farming, living soils, and permaculture provided by Jeff Lowenfels.

The Hydroponics chapter has been revised and expanded by Neil Mattson, PhD, professor at Cornell University.

Basic small- and large-scale indoor garden setups were written by Bill Faulconer, author of Design & Build a Room to Grow: Money Can Grow on Trees, with scaling considerations provided by Justin Arriola of Automated Growth Solutions. Extra considerations for indoor design optimization and electrical safety were provided by Joey Ereñeta of Oaksterdam University.

In the last part of this section we have included a showcase of alternative gardens, growing styles, and strategies from growers we admire: the late Dennis Peron, Dan Vinkovetsky (formerly Danny Danko of High Times), Daniel Old E and Brian Bleezy Eatmon of Mendo Dope and Mark Grayshock of Greenshock Farms, Remo aka @UrbanRemo, Sunshine Cereceda of Sunboldt Grown, Tyler LeBlanc of Apollo Green, Kristin Nevedal of the International Cannabis Farmers Association, Autumn Shelton and the Brand Family of Autumn Brands, Jeremy Babbitt of 918 Oklahoma Grown and Andy Unruh of Sticky Flower Farm, Trent Hancock of Creswell Oreganics, Marty and Laura Clein of Martyjuana, Wendy Kornberg of Sunnabis, and Chris Trump. These profiles were written by the authors unless otherwise noted.

Finally, for the first time we have included an overview of concepts of sustainability, for all types of gardens. This content was provided by Dale Sky Jones, chancellor of Oaksterdam University, and other Oaksterdam faculty, with supplemental content by the authors.

Part IV: The Plant Life Cycle focuses on each stage of the plant’s life cycle: propagation, vegetative growth, and flowering. These chapters were written by the authors and edited by Jay Kitchen of Uptown Growlab. Jay is the author of Uptown Growlab’s The Kitchen and the 2021 Cannabis Annual . After a career as an administrative law judge in New York City, Jay came out of the cannabis closet and relocated to the Pacific Northwest, where he hosts the popular Uptown Growlab live show on YouTube.

Finishing and flushing products and processes are covered by the authors.

Part V: Harvesting & Processing details the harvesting process, how to choose a harvesting date, and all the different ways the crop is processed after it has been cut down.

The process of ripening is covered by the authors, with original study on the evolution of the chemical profile found in the trichomes throughout the flowering process by Caleb King, Thomas Blank, PhD, and Reggie Gaudino, PhD, of Front Range Biosciences, and Kymron DeCesare and Donald P. Land, Ph.D, consultants to Front Range Biosciences.

Picking, trimming, drying, curing, and storing were written by the authors with editorial review by Clayton Stewart, an environmental technologist, post-harvest specialist, and consultant who has worked with large-scale commercial facilities in Canada. Clayton also wrote the Automation chapter and originated a study on drying and curing to expand this section.

To close out this section, the authors cover solventless extraction and concentration methods.

The appendices cover propagation methods: cloning, tissue culture, producing seeds, and regeneration, as well as a pest and disease guide designed for problem-solving in the garden.

The Pest & Disease appendix was revised by integrated pest management expert Saul Alba. The Cloning appendix was revised by Ellen Holland. The Producing Seeds appendix was written by the team at Humboldt Seeds.

The guide to tissue culture was written by Bill Graham of Microclone Tissue Culture. Bill is a founding member of the Cannabis Group of the Society of In Vitro Biology.

Finally, we have included a list of incredible garden consultants who contributed to the production of this book, a thorough glossary, and extensive bibliography and resources section.

While the laws, tools, and techniques will continue to evolve rapidly after the publication of this edition of Ed’s best-selling cultivation guide, now the Cannabis Grower’s Handbook, the fundamentals will never change. We hope that new and experienced growers who pick up this book will continue the legacy of innovation and social change that inspired the readers of the first and subsequent editions.

In the introduction to the 2010 edition Ed warned, Using marijuana isn’t addictive but growing it is. Over the last 50 years cannabis has become the gateway to gardening for many people in many more places. Although cannabis will not be truly legal until everyone has the right to grow their own, we hope the publication of this new edition leads to further research, understanding, and freedom of this world-changing plant.

Foreword to Marijuana Grower’s Guide, 1974

Over the past 10 years there have been revolutionary changes in the values of young people. The empty materialism of the fifties and liberal idealism of the sixties have been washed away by a pragmatic re-evaluation of lifestyle and political structure.

To a great extent this is the result of the widespread use of the psychoactive herbs and drugs which burst upon the scene in 1967. These substances seem to break down the ego and defense mechanisms and allow individuals to re-evaluate the sets and set perceptions based upon behavior patterns no longer relevant.

Marijuana, the most popular of the psychoactive herbs, has helped millions of people to a broader understanding of themselves. It is for precisely this reason that governments all over the world view it as a dangerous drug. How can they control their people if they see through the hypocrisy and self-serving purpose of the leaders’ actions?

The use of marijuana has become so widespread that the government’s repressive efforts, such as Operation Intercept, have resulted in almost total failure, and contempt for the inept efforts by the rulers of a system which hears its death-knell but does not understand the sounds.

However, the economic system which makes marijuana seem like just another commodity, rather than the sacrament that it should be, must be replaced. This book will help you make marijuana free.

FREE GRASS   FREE YOURSELF   FREE THE WORLD

PART I:

THE CANNABIS PLANT

UNDERSTANDING THE EFFECTS OF CANNABIS

by Gregory Gerdeman, PhD

Cannabis and the Endocannabinoid System

For thousands of years, cannabis followed humans, carried from one civilization to the next as a cherished plant companion.

The human-cannabis relationship is a powerful example of what biologists call mutualism, when two species mutually benefit the survival of the other. By selecting for the traits that breeders most valued, humans shaped cannabis evolution and continue to do so. In turn, this companion plant has contributed greatly to human societies through its use for food, clothing, shelter, medicine, manufacturing, religion, recreation, and other aspects of commerce and culture (Clarke and Merlin 2013).

It has been hypothesized that modern humans coevolved with cannabis. The implication is that cannabis has helped shape not only our cultural history but also our genetic code, the underlying biology of who we are (McPartland and Guy 2004). This is an extraordinary idea, that during the natural history of human cannabis use the relationship changed humans in a heritable way and thus affected the evolutionary trajectory of our species.

What is known for sure is that the reasons we have cultivated cannabis as a beneficial companion are inherent in our biology and helped create our evolutionary success. In a manner of speaking, we were predisposed to the relationship well before we became ecologically entwined. It is inherent in the biochemistry of how our bodies function and how cannabis touches us at the cellular level.

It is quite evident from history, as well as the present-day resurgence of cannabis as medicine, that cannabis enhances human survival and helps protect from injury and disease. Cannabis is a medicine that improves quality of life. The biologically active metabolites of cannabis support human health at molecular and cellular levels by acting at a physiological mechanism called the endocannabinoid system, which evolved as a key component of animal, and thus human, biology (Pellerin 2020).

Cannabis, The Medicine Flower

Some of the earliest discovered examples of written language describe cannabis as a medicine, as do medical texts from prominent cultures that flourished in the intervening millennia. Cannabis use since antiquity was widespread, and it was not just low-THC hemp. Archaeological evidence, supported by modern lab tests, shows that in parts of the ancient world that were quite distant from one another, ritual-medicinal uses of THC-rich cannabis not only existed but were revered and sacred (Russo 2007).

Before the invention of writing, cannabis was recognized as a vital crop unlike any other because of its multitude of uses for survival. But it’s the truly extraordinary flower, distinctive among all others in nature, that is the reason there is such interest in the plant.

The many uses of cannabinoids found on these flowers range from lifting the mood to relaxing spastic muscles and even brain seizures. Cannabis eases aches and pains and can safely amplify the analgesic effects of other, more dangerous painkillers. It can distract thoughts from the passage of time, helping us forget, while often enhancing the emotional joys of exercise, sex, and eating.

In the decade since the previous edition of this book, there has been an explosion of scientific discovery about how cannabis provides such a broad range of therapeutic and enjoyable effects. To understand it, consider what each side of this relationship brings to the table. What is in cannabis that affects us so? What is it in humans that cannabis interacts with?

Cannabinoids

Cannabis produces well over 100 structurally related chemical compounds called cannabinoids. The production of these cannabinoids occurs primarily in and around the tightly clustered flowers that are the pride and joy of sinsemilla growers. The so-called bud is actually a leafy inflorescence, and it is covered with nearly microscopic, bubble-like trichome glands filled with a sticky, cannabinoid-rich resin. These glandular trichomes of cannabis are more than simply photogenic; they are marvels of chemical synthesis, creating a host of metabolites that supports the survival of the plant.

The most abundant of the cannabinoids are Δ⁹ tetrahydrocannabinol (THC) and cannabidiol (CBD). These are the superstars that drive the psychotropic and medical uses of cannabis. THC and CBD are truly sister molecules, derived from the very same parent compound, the mother cannabinoid cannabigerol (CBG). More accurately, the true parent precursor is the raw or acid form, cannabigerolic acid (CBGa), which is converted to THCa or CBDa (or to a lesser extent CBCa) in a genetically controlled process (de Meijer 2016).

Like fraternal twins, THC and CBD share some characteristics but are definitely not identical and do not behave the same. THC is profoundly psychotropic and is the driver of the cannabis high. This, of course, is very well known, and for decades following the elucidation of THC’s chemical structure in the early 1960s, most of the world assumed that Δ⁹ THC was the entire story behind why people enjoy using cannabis. This was validated by the observation that pure THC oil replicates the hashish high using no other components (Mechoulam and Ben-Shabat 1999).

THC is the dominant psychotropic player in cannabis, but can pure THC faithfully replicate a full cannabis experience? No. This is a simplification. Behavioral measures don’t need to be very sophisticated to show that a shot of straight THC can get a human highly stoned or render a lab rat unable to move. The isolated focus on THC does not take into account the entourage effect, where other cannabinoids or terpenes affect the experience too and lead to a spectrum of varying effects on people, not all of them psychotropic (Russo 2011).

In the glandular trichomes of cannabis, the mother cannabinoid CBG is converted either to THC, CBD, or CBC in different amounts based on competing enzyme activities. The different cannabinoid ratios found in cannabis are mostly due to genetic variation in these cannabinoid synthase enzymes. Selective breeding has led to cannabis varieties that make neither THC or CBD, accumulating high levels of CBG instead. CBC is a lesser-known daughter of CBG. Although CBC is a minor component of contemporary cannabis, it is part of the therapeutic cannabinoid ensemble, and breeding efforts will generate more CBC-rich varieties.

SOURCE: Steep Hill Lab and Elemental Wellness.

What is a cannabinoid?

There is inconsistency out there about when to use the term. Originally a moniker for secondary metabolites unique to cannabis, today it tends to define function more than origin, referring to those molecules that are active at cannabinoid receptors. In addition to endocannabinoids described in this chapter, there are synthetic cannabinoids constructed by chemistry labs for research and pharmaceutical drug development. Some have turned into illicit products sold as Spice, K2, and other names. On the other hand, the natural terpene β-caryophyllene has been called a cannabinoid because, like THC, it stimulates CB2 cannabinoid receptors (but not CB1), thereby representing a clear example of the herbal entourage effect inherent to cannabis (Gertsch et al. 2008). It is not a classic cannabinoid in structure, and is common not only in cannabis but in the essential oils of other dietary herbs such as black pepper, cloves, and copaiba. Some prefer to call caryophyllene a cannabimimetic, a name used for non-cannabinoid molecules that are known to act on the human ECS, such as compounds found in frankincense, liverwort, and echinacea (McPartland et al. 2014).

The therapeutic properties of the other twin, CBD, alleviate inflammation and consequent pain states, relieve anxiety and psychosis, decrease seizure frequency, and alleviate muscle spasms. Bold as these claims are, they are supported by many high-quality preclinical scientific studies, as well as numerous patient surveys and clinical anecdotes (Crippa et al. 2018). There is evidence, however, that CBD works more effectively in the presence of THC and/or other components present in full-spectrum cannabis extracts (Gallily et al. 2015; Sulak et al. 2017).

Minor Cannabinoids

Of the over 100 unique cannabinoids, very little about the pharmacology of most of them is known, but some of the more prominent minors are of great interest and may well be important players in commercially available cannabis. One key example is CBG, the mother cannabinoid, which in 2021 is being grown in abundance thanks to successful breeding to develop high-CBG flowers. Both CBG and cannabichromene (CBC) influence the cannabis high and have therapeutic properties ranging from inhibiting tumors to bacterial infections (Russo and Marcu 2017).

Also present in cannabis is cannabinol (CBN), which is merely an oxidative breakdown product of THC, but is now produced in labs more than it occurs in nature. Similarly, Δ⁸-THC is a natural isomer of THC that only minimally occurs in the flower but has recently garnered interest because it can be easily lab-manufactured through a chemical conversion of CBD. Both Δ⁸-THC and CBN act relatively weakly at some of the same cellular receptors described later in this chapter (Pertwee and Grazia Cascio 2014) and are sometimes called THC light. These molecules are surging in popularity, but it is important to realize that the chemistry that creates Δ⁸ -THC, Δ¹⁰-THC, or even CBN also generates other by-products, depending on the precise conditions of the chemical reaction. Quality control is critical if these products are to be trusted for long-term use.

Breeding programs have also brought out increasing concentrations of the varin cannabinoids: THCV and CBDV (Lewis et al. 2018). These are also called propyl cannabinoids based on the 3-carbon tail in their molecular structures. They have numerous influences on the psycho-therapeutic properties of any flower that contains them. As refined drugs, both are in clinical studies.

Last but not least, the raw acid forms such as THCa and CBDa, which are what actually occur in the fresh, unheated plant material, have a variety of biological activities that make them compelling players in the ways that cannabis can support human health and well-being (Russo and Marcu 2017). Calling the cannabinoid acids minor is a bit funny, since they are what the plant actually makes, but in most all of the ways that cannabis is processed or heated during consumption, the acids are efficiently decarboxylated (decarbed) into the better-known forms.

The Terpene Entourage

The range of fragrances that cannabis produces is well known. They are produced in the same place as cannabinoids, in the glandular trichomes. The diverse palate of scents to be found in cannabis is not due to cannabinoids, which have no odor, but to aromatic terpenes.

Unlike cannabinoids, terpenes are not unique to cannabis at all but are also abundant in most other plants. Most flower odors and other fragrant resins are composed of terpenes. The therapeutic properties of medicinal herbs, and their essential oils that are used for perfumes, flavorings, and aromatherapy, are often due to the terpenes they contain.

An important concept is that this natural ensemble of botanical metabolites, the entourage of cannabinoids and terpenes found in whole cannabis, work together to support health in ways that are superior to single molecules acting alone as isolated drugs.

It has long been appreciated by cannabis enthusiasts that the smell of the flower can, to some degree, predict the nature of the high it will elicit. The aroma is therefore linked to variation in biological effect. Other evidence comes from clinical studies that found that whole-plant extracts may produce superior outcomes than isolated ingredients (Gallily et al. 2015; Blasco-Benito. et al, 2018).

In addition to shaping the taste and smell that may distinguish one variety of cannabis from another, the bouquet of terpenes creates the entourage effect that determines, together with the cannabinoids and other bioactive molecules, the shape and feel of the cerebral experience, or the specific medicinal value.

Flavonoids

While the floral, skunky, and fuel smells of cannabis are famous, people who do not cultivate the plant are often surprised to learn that it can also be quite colorful. Like many plants, when shorter light cycles begin to diminish chlorophyll levels, the dominant green progressively gives way to underlying hues of yellow, red, and purple. The pistil hairs and even tiny trichome heads take on an orange coloration. In some varieties, the flower itself naturally matures into a striking purple, which is often reflected in the cultivar name. In addition, most any cannabis plant will turn purplish if it is shocked by cold temperatures.

Anthocyanins and other flavonoids bring out deep purples, fall colors and health-promoting properties in cannabis. Photo: Dr. Greg Gerdeman

All these colorations are the product of pigment molecules called flavonoids. Aside from aesthetics, flavonoids are an important part of the health benefits of fruits, berries, vegetables, and cannabis. Often called polyphenols, which is a reference to their molecular structure, they are not known to be psychotropic or to affect the cannabis high in any way.

Flavonoids are strong antioxidants, which all by itself could contribute to an entourage effect of enhancing well-being. Research has indicated that low dietary intake of flavonoids is associated with increased risk of cardiovascular disease and cancers, suggesting that a diet rich in flavonoids may be protective. Other experiments have found that flavonoid consumption enhances healthy blood flow to the brain (Rees et al. 2018). Most of the flavonoids in cannabis are common in other plants, such as the anthocyanins that are responsible for those purple colors. Cannabis also has at least two unique flavonoids called cannflavin A and B, which have been shown to have potent anti-inflammatory properties (Russo and Marcu 2017).

How does the entourage of cannabinoids, terpenes, and flavonoids team up? It is known that from one chemotype (chemical profile) to the next, the subjective experience of a high can be very different. What about cannabis as medicine? Are there distinct metabolite profiles in certain varieties that make them more effective than others for treating particular symptoms or diseases? The internet abounds with such claims, but the evidence behind it is mostly very poor, confounded by inaccurate product labeling, inconsistent dosing, and the inability to set up clinical trials to control the many variables of an entourage study, including placebo effects. The truth is still unknown. To date, there is not enough science to clearly prove such a wishful logic, but there are plenty of reasons to hope that a full exploration of the bioactive ensembles in cannabis could lead to this kind of understanding.

Having given this introduction to the complexity that cannabis brings to the relationship, attention must now be paid to the biochemistry and physiology of humans, and the ways in which humans have evolved to respond to cannabis. Here again, the story is much richer than imagined until recent years.

The Endocannabinoid System (ECS)

The key to it all — how cannabis alters physical and mental states and eases symptoms of so many injuries and illnesses — is a complex, integrative mechanism in human bodies called the endocannabinoid system (ECS). The ECS regulates vital cellular activity of animal bodies. It works to protect against toxic stresses, to promote homeostasis (which means a normal physiological balance), and to support general health and well-being. Undetected until the very end of the last century, the biology of the ECS provides a scientific rationale to explain humanity’s innate therapeutic use of cannabis.

The ECS consists of:

Various cannabinoid receptors used by cells to communicate, maintain balance, and sense the outside (and internal) world. They are activated by cannabinoids in cannabis.

The endocannabinoids (eCBs), signaling molecules produced within the body that act on those receptors, as a way that cells communicate with one another to maintain homeostasis.

The ECS enzymes that either create or break apart the endocannabinoids. Changing the function of these enzymes dictates how many endocannabinoids are present and available in any given place and time within the brain and body.

Signaling molecule is a general term for chemicals used by cells to communicate within the body. For example, neurotransmitters are signaling molecules used by brain cells (neurons) to communicate. Endocannabinoids are used not only by neurons but by nearly every type of cell in the body.

The endocannabinoid system is referred to as a master regulator of human physiology.

The first big breakthrough came in 1988 and following years, when scientists discovered that there are receptors on the surface of brain cells that grab onto THC as though they evolved just to do so (Devane et al. 1988). These molecular locks, unlatched and activated by THC, quite naturally earned the name cannabinoid receptors because they were found through a deliberate search to decipher the secrets of cannabis’s psychotropic effects.

Two subtypes were later recognized, encoded by different (but similar) genes, and expressed in different preferred areas of the body. These receptor proteins were called CB1 and CB2 (Herkenham et al. 1990; Munro et al. 1993).

The CB1 Receptor: A Choreographer of the Brain

Endocannabinoids in the Nervous System

The ECS is a master choreographer of nervous system activity. Neurons release endocannabinoids (eCBs) from their cellular membranes, which act at CB1 receptors in order to dampen down or fine-tune synaptic connections between brain areas. Brain circuits related to pain, emotional regulation, appetite, and higher thinking are all highly regulated in this way. This partly explains many of the most recognized actions of THC, which boosts ECS function as an outside CB1 activator. Some neurons of the cerebral cortex even use eCBs to self-regulate their own electrical activity, which is believed to play an important role in generating certain brain rhythms. Even target cells in the body use eCBs to give feedback to inputs from the nervous system, thereby shaping autonomic responses to stress, for example.

Graphic: Dr. Greg Gerdeman

THC affects mood, senses, and clarity of memory. Under the influence of cannabis, one might perceive the passage of time differently. Anxious thoughts can be replaced by sensory attention to something funny in the here and now, whether it is something mundane or something profound. Appetite may increase, while motor coordination may fall off. All of these are effects of THC working at CB1 receptors in different areas of the central nervous system, which includes the brain and spinal cord.

The human brain contains hundreds of different kinds of receptors that respond to dozens of neurotransmitters and hormones. It is part of the cellular biochemistry that makes this gray blob behind the forehead the most complex structure in the known universe. Of all those receptors, none are more prominent than the CB1 cannabinoid receptor.

The brain is literally teeming with CB1 receptors it uses to choreograph the rhythm of the circuits and keep feedback loops functioning in a healthy way that integrates brain with body, experience, and intention.

Of course, no cannabis is required for this to be so special and so important for biology. Animal bodies make their own endocannabinoids (eCBs), a shortened term for endogenous (internal) cannabinoids. The name itself reflects that the endocannabinoids are very similar to THC, the phytocannabinoid (from the plant) that showed their existence. The first endocannabinoid discovered was named anandamide by its discoverer, from the Sanskrit word ananda, meaning internal bliss, a wonderful homage to the beneficial properties of cannabis (Devane et al. 1992). A second endocannabinoid that is actually more abundant is 2-AG. Anandamide and 2-AG are the actual playmakers used by cells throughout the brain and body to promote homeostasis by acting at cannabinoid receptors CB1 and CB2.

How is it that the ECS acts as a master regulator, or conductor of the neural symphony? Neurons in the brain release endocannabinoids to regulate and fine-tune their own inputs (Gerdeman 2007).

Consider what happens when a brain suffers a seizure, as in the case of someone with epilepsy or acute brain injury. Neurons are electrically active cells, and they are arranged in circuits in the brain. In a seizure, those circuits go haywire, barraging neural receptors with excessive electrical activity, one cell jolting the next with waves of excitatory neurotransmitters that push the cycle forward. The seizure ripples in waves across the brain, causing muscle spasms, blackouts, and the various outward symptoms of losing control over one’s body.

CB1 receptors normally help prevent this from happening. When a cell starts receiving a surge of excitatory stimulus, for whatever normal reason, it responds by quickly generating endocannabinoids and releasing them to the signal producer. This is called retrograde signaling. They bind to the original sender neuron’s CB1 receptors that are mostly found on the end-tips of the neurons, where the release of neurotransmitters occurs.

In this way, when a cell is receiving excessive inputs that are pushing it to be more electrically active than it should normally be, the release of endocannabinoids provides a negative feedback loop that says, Slow down, and returns the system to balance (Katona and Freund 2008).

With epilepsy, stroke, and other brain injuries, the therapeutic implications for cannabis are really quite clear. The endocannabinoid system is protective, part of the brain’s frontline defense against excitotoxicity damage. By directly stimulating CB1 receptor activity, cannabis is a supplement for the ECS, a boost for the endocannabinoids that need help to control the situation.

Cannabinoids and terpenes interact within the brain to influence the neural circuits of sensation and mood, including for the treatment of injury and disease.

CB1 is found virtually everywhere, regulating neural activity to keep it in balance. When pain-transmitting nerves carry their signals to the brain, healthy CB1 receptors help to keep the signal in check so that pain is appropriate, not pathological.

When the brain perceives a threat, the ECS acts through CB1 receptors to keep an initial fear response, and the momentary stress that motivates a proper fight-or-flight response, from turning into debilitating and chronic stress. Even the ability to forget or move past a traumatic experience, called fear extinction, involves a well-studied mechanism of endocannabinoids acting at CB1 receptors in the so-called fear center of the brain, the amygdala (Morena et al. 2016).

The CB2 Receptor: A Thermostat of the Immune System

Whereas CB1 is most densely but not exclusively found throughout the nervous system, the CB2 receptor is found on the surface of cells that make up the immune system. Crawling and circulating throughout the body, the immune system is also enormously complex, with moving parts. Communication is key, and here again, the ECS plays a master role to regulate immune system communication and activity. Endocannabinoids act primarily at CB2 receptors to turn down excessive inflammation and to prevent tissue-damaging outcomes that can occur when an amped-up immune system starts to turn on its own body (Maccarrone et al. 2015).

Just as neurons release neurotransmitters to communicate, and how endocrine glands release hormones to send signals throughout the body via the bloodstream, immune cells communicate through the release of signaling molecules called cytokines. Many of these cytokines are pro-inflammatory, meaning that they stimulate a ramped-up immune response that recruits more and more first responders to the scene of an injury or infection. This leads to swelling, fever, and feelings of malaise that help to keep a person off their feet to allow for recovery. Just as CB1 receptors dial down the release of neurotransmitters in the brain, CB2 receptors respond to cannabinoids by inhibiting the release of pro-inflammatory cytokines.

The ECS: A Cellular Mechanism of Integrative Physiology

What is called an endocannabinoid system is more accurately a fundamental cellular mechanism. It isn’t that some cells make up the ECS and others don’t. Nor is it just in the brain and the immune system. Rather, cells of every tissue type utilize this mechanism. Some cells release endocannabinoids, others respond to them, often doing both. This ECS is integrative because it assists in the proper function of all physiological systems, helping to promote balance and integrate systems into the healthy balancing act that makes up a whole human being.

The ECS is an Entourage Too!

CB1 and CB2 receptors are important players in maintaining human health, and their discovery opened the door to reintroducing cannabis as an accepted part of medicine, yet they are not the whole story to the extended ECS and how the entourage of cannabis exerts its effects. It turns out that there are numerous other ways eCBs work, involving other receptor targets, which also promote pathways of maintaining homeostasis.

First are the TRP channel receptors. This important class of cell surface ion channels allows cells to monitor and respond to their sensory environment (Kaneko and Szallasi 2014). Depending on the subtype, TRP channels are activated by a number of physical stimuli such as temperature, acidic pH, osmotic swelling, or other mechanical stretching. They are also activated by a variety of essential oils from culinary and therapeutic herbs. The TRPV1 channel, for example, is activated by capsaicin from hot chili peppers. It is literally responsible for why humans feel pain from heat. Ever wonder why hot cream rubs are used for topical pain relief? It is because after the capsaicin creates a hot sensation, it stays stuck to the TRPV1 channel and causes it to desensitize. It is the same reason one can adjust to hot spicy food after the first few bites. When researchers searched for an endogenous activator for this hot pepper receptor, they were surprised to learn that it is none other than anandamide and 2-AG. Unlike capsaicin, the eCBs — and also CBD (but not THC) — simply desensitize the receptor rather than allowing it to open. CBD topicals do not feel hot, but their mechanism of pain relief is directly related to hot rubs. Recent research found that β-myrcene also inhibits TRPV1 activity (Jansen et al. 2019). This is only one example out of at least six different TRP channels that are known to be modulated by other phytocannabinoids and terpenes in vitro (experiments using cells in a petri dish; Muller et al. 2019).

Second, a pair of proteins called PPARs have long been studied as regulators of metabolism, and they too are regulated by both eCBs and phytocannabinoids (O’Sullivan 2016). In particular, PPARγ (gamma) is a so-called nuclear receptor because when it is activated, it moves into the nucleus of the cell and turns on genes associated with improving metabolism. PPARγ is activated by a class of drugs called glitazones, which are FDA-approved for the control of type 2 diabetes. Animal research has found both CBD and THCa, and likely others, can similarly activate PPARγ. This may help to explain repeated epidemiological findings that cannabis users tend to have a lower body mass index compared to non-users (Alshaarawy and Anthony 2019).

The ECS Discovery is Truly Revolutionary

The scale of influence the ECS exerts as a regulator of homeostasis at levels all over the body is a uniquely impressive aspect of human biochemistry. What we call an endocannabinoid system is more accurately a fundamental and distributed cellular mechanism.