In tests on four genomes, it generated the longest contigs among all assemblers tested. It exploited the mate constraints provided by paired-end reads from ...
Dec 15, 2008 · In tests on four genomes, it generated the longest contigs among all assemblers tested. It exploited the mate constraints provided by paired-end ...
In tests on four genomes, it generated the longest contigs among all assemblers tested. It exploited the mate constraints provided by paired-end reads from ...
Dec 15, 2008 · DNA sequence reads from Sanger and pyrosequencing platforms differ in cost, accuracy, typical coverage, average read length and the variety of ...
People also ask
What are the limitations of pyrosequencing?
No system is perfect, and the main problems with Pyrosequencing include the need for expensive biotinylated primers, and the inability to accurately detect variants within long (>5 or 6 bp) homopolymer stretches.
What are the stages of pyrosequencing?
The pyrosequencing method involves four main stages: first, target DNA is amplified using PCR; second, double-stranded DNA is converted to single-stranded DNA templates; third, oligonucleotide primers are hybridized to a complementary sequence of interest; and, finally, the pyrosequencing reaction itself, in which a ...
What is the read length of pyrosequencing?
Typical reading length using pyrosequencing technology is 40−60 bases. However, as with any sequencing technology, the maximum read length will depend on template secondary structure, base content, quality of PCR-product, and other parameters.
What chemical compound would be detected in pyrosequencing?
Pyrosequencing is based on the detection of a pyrophosphate molecule released following the activity of the DNA polymerase in a reaction mixture that also contains a single-strand DNA molecule, an annealed primer, and an enzyme cascade system to produce light.
Abstract Motivation: DNA sequence reads from Sanger and pyrosequencing platforms differ in cost, accuracy, typical coverage, average read length and the ...
[PDF] Supplement Miller et al. 2008 “Aggressive Assembly of ...
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2008 “Aggressive Assembly of Pyrosequencing Reads with Mates”. Data sets that included both 454 FLX reads and Sanger reads were selected to test the assemblers.
Oct 5, 2023 · A concrete guideline for the construction of haplotype representation according to the degree of heterozygosity, followed by polishing and purging haplotigs.
Oct 21, 2011 · Comparative analysis of algorithms for whole-genome assembly of pyrosequencing data ... Aggressive assembly of pyrosequencing reads with mates.
Mar 8, 2021 · Here, we introduce the assembler SMARTdenovo, a single-molecule sequencing (SMS) assembler that follows the overlap-layout-consensus (OLC) paradigm.
Sep 15, 2013 · Recommendations. Aggressive assembly of pyrosequencing reads with mates. Motivation: DNA sequence reads from Sanger and pyrosequencing ...