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Combining Ultrasound-Mediated Intracellular Delivery with Microfluidics in Various Applications

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Abstract

Ultrasound-mediated intracellular delivery is one of the popular technologies based on membrane rupture at present. To date, ultrasound directly acts on a large number of cells to achieve cargo delivery and has been widely used in drug delivery, disease therapy and other fields. However, the existing macroscopic methods can no longer meet the requirements of accurate tracking and analysis and are prone to extensive cell damage and even death. With the rapid advancements in microfluidic technologies, the combination of ultrasound and microfluidics (CUM) technology can effectively improve the delivery efficiency and cell survival rates. This new technology has rapidly become a new direction and focus of research. Thus, we analysed the mechanism of sonoporation and the effect of acoustic waves in a microfluidic channel. In addition, we reviewed the application of these new technologies in terms of structure and fabrication of ultrasound transducers and microfluidic devices. As regards our main objective, we hope to help researchers better understand the future developments and the challenges of new technologies. With this review, researchers can promote the development of new technologies to solve the current challenges of intracellular delivery and advance clinical applications.

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Acknowledgements

This research was funded by the Guangxi Bagui Scholars Project (Grant No. 2019A02), and the Interdisciplinary Scientific Research Foundation of GuangXi University (Grant No. 202200245), and the Guangxi Key Laboratory of Manufacturing System Advanced Manufacturing Technology (Grant No. 15-140-305005), and the Interdisciplinary Scientific Research Foundation of GuangXi University (Grant No. 2022JCB006).

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  1. Key Laboratory of Disaster Prevention and Structural Safety of Ministry of Education, Guangxi University, Nanning, 530004, China

    Guangyong Huang, Lin Lin, Ying Liu & Hui You

  2. School of Mechanical Engineering, Guangxi University, Nanning, 530004, China

    Guangyong Huang, Lin Lin, Shixiong Wu, Haojie Dang, Xuesong Cheng & Hui You

  3. School of Mechanical and Automotive Engineering, Guangxi University of Science and Technology, Liuzhou, 545006, China

    Guangyong Huang

  4. Guangxi Key Lab of Manufacturing System and Advanced Manufacturing Technology, Nanning, 530003, China

    Lin Lin

  5. Guangxi Key Laboratory of Disaster Prevention and Engineering Safety, Guangxi University, Nanning, 530004, China

    Ying Liu & Hui You

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Huang, G., Lin, L., Wu, S. et al. Combining Ultrasound-Mediated Intracellular Delivery with Microfluidics in Various Applications. BioChip J 18, 22–44 (2024). https://doi.org/10.1007/s13206-023-00128-w

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