Abstract
Consumer concerns and government regulations focused on the safety of foods dictate the need for analysis of various food contaminants, residues, and chemical constituents of concern. These compounds include pesticide residues, mycotoxins, antibiotic residues, GMOs, allergens, food adulterants, packaging material hazardous chemicals, environmental contaminants, naturally occurring substances formed during processing, among others. This chapter covers the screening methods and quantitative methods that are commonly used for the detection and quantification of several food hazards, in addition to some recently developed methods for the detection of newly identified and emerging food hazards. Included are both rapid screening methods and more time-consuming quantitative methods required to meet the needs of industry and government, in an effort to ensure a safe and reliable food supply. Sampling and sample preparation are covered because they can be a significant challenge due to the low levels of the analytes and the complexity of food matrices. Methods referred to in this chapter include many techniques covered in other chapters (e.g., high-performance liquid chromatography, gas chromatography, mass spectrometry, immunoassays, enzyme inhibitor assays), and others not previously covered (DNA methods using polymerase chain reaction, certain colorimetric assays). This chapter is intended to compliment Chap. 35 on food forensic investigations.
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References
Nielen MWF, Marvin HJP (2008) Challenges in chemical food contaminants and residue analysis, Ch. 1. In: Pico Y (ed) Food contaminants and residue analysis. Comprehensive analytical chemistry, volume 51. Elsevier, Oxford, UK
Arvanitoyannis IS (2008) International regulations on food contaminants and residues, Ch. 2. In: Pico Y (ed) Food contaminants and residue analysis. Comprehensive analytical chemistry, volume 51. Elsevier, Oxford, UK
FDA (2023) Chemical Contaminants & Pesticides. https://www.fda.gov/food/chemical-contaminants-pesticides
FDA (2023) Food Chemical Safety. https://www.fda.gov/food/food-ingredients-packaging/food-chemical-safety
European Union (2020) Rapid alert system for food and feed. Preliminary annual report. https://food.ec.europa.eu/system/files/2021-08/rasff_pub_annual-report_2020.pdf
Alweera A, Khursheed N, Samra F, Anjum Z, Younis K (2022) Application of nanotechnology in food packaging: Pros and Cons, J Agric Food Res 7:100270
US Environmental Protection Agency (Accessed Sep 2023) Safety and Waste Management for SAM Biotoxin Methods https://www.epa.gov/esam/safety-and-waste-management-sam-biotoxin-methods
Lee J, Kim L, Shin Y, Lee J, Lee Jiho, Kim E, Moon J, Kim J (2017) Rapid and Simultaneous Analysis of 360 Pesticides in Brown Rice, Spinach, Orange, and Potato Using Microbore GC-MS/MS. J Agric Food Chem, 65:3253–3430
Turnipseed SB, Andersen WC (2008) Veterinary drug residues, Ch. 10. In: Pico Y (ed) Food contaminants and residue analysis. Comprehensive analytical chemistry, volume 51. Elsevier, Oxford, UK
Díaz-Bao M, Barreiro R, Miranda JM, Cepeda A, Regal P (2015) Fast HPLC-MS/MS method for determining penicillin antibiotics in infant formulas using molecularly imprinted solid-phase extraction. J Anal Methods Chem 2015:1–8
Sannino A (2008) Pesticide Residues, Ch. 9. In: Pico Y (ed) Food contaminants and residue analysis. Comprehensive analytical chemistry, volume 51. Elsevier, Oxford, UK
Stachniuk A, Fornal E (2016) Liquid chromatography-mass spectrometry in the analysis of pesticide residues in food. Food Anal Methods 9:1654–1665
De Santis B, Debegnach F, Gregori E, Russo S, Marchegiani F, Moracci G, Brera C (2017) Development of a LC-MS/MS method for the multi-mycotoxin determination in composite cereal-based samples, Toxins (Basel) 9:169
Sandra P, David F, Vanhoenacker G (2008) Advanced sample preparation techniques for the analysis of food contaminants and residues, ch. 5. In: Pico Y (ed) Food contaminants and residue analysis. Comprehensive analytical chemistry, volume 51. Elsevier, Oxford, UK
Lehotay SJ, Cook JM (2015) Sampling and sample processing in pesticide and residue analysis. J Agric Food Chem 63:4395–4404
Anastassiades M, Lehotay SJ, Stajnbaher D, Schenck FJ (2003) Fast and easy multiresidue method employing acetonitrile extraction/partitioning and “dispersive solid-phase extraction” for the determination of pesticide residues in produce. J AOAC Int 86:412–431
Niell S, Cesio V, Hepperle J, Doerk D, Kirsch L, Kolberg D, Scherbaum E, Anastassiades M, Heinzen H (2014) QuEChERS-based method for the multiresidue analysis of pesticides in beeswax by LC-MS/MS and GC×GC-TOF. J Agric Food Chem 62:3675–3683
Kinross AD, Hageman KJ, Doucette WJ, Foster AL (2020) Comparison of Accelerated Solvent Extraction (ASE) and Energized Dispersive Guided Extraction (EDGE) for the analysis of pesticides in leaves. J Chroma A, 1627:461414
Jiao, Z, Guo Z, Zhang S, Chen H (2015) Microwave-assisted micro-solid-phase extraction for analysis of tetracycline antibiotic in environmental samples. Int J Environ An Ch 95:82–91
Merkle S, Kleeberg K, Fritsche J (2015) Recent Developments and Applications of Solid Phase Microextraction (SPME) in Food and Environmental Analysis—A Review. Chromatography, 2:293–381
EPA (2022) Pesticide Product Information System. https://www.epa.gov/ingredients-used-pesticide-products/pesticide-product-information-system-ppis
EPA Basic Information about Pesticide Ingredients (2023) https://www.epa.gov/ingredients-used-pesticide-products/basic-information-about-pesticide-ingredients
FDA (2002) Pesticide analytical manual volume II (updated January, 2002). http://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm113710.htm
FDA (1999) Pesticide analytical manual volume I (PAM), 3rd edn (1994, updated October, 1999). http://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm111455.htm
AOAC International (2023) Official methods of analysis (on line), 22nd edn. Official methods of analysis, 22nd edn. AOAC International, Gaithersburg, MD
Tadeo JL (2008) Analysis of pesticides in food and environmental samples. CRC, Taylor and Francis Group, Boca Ranton, FL
Lee NA, Kennedy IR (2001) Environmental monitoring of pesticides by immunoanalytical techniques: Validation, current status, and future perspectives. J AOAC Int 84:1393–1406
Soderberg D (2005) Committee on residues and related topics; pesticides and other chemical contaminants. General referee reports. J AOAC Int 88:331–345
Pico Y, Blasco C, Font G (2004) Environmental and food applications of LC tandem mass spectrometry in pesticide-residues analysis: an overview. Mass Spectrom Rev 23:45–85
Patel K, Fussell RJ, Goodall DM, Keelye BJ (2004) Evaluation of large volume-difficult matrix introduction-gas chromatography-time of flight-mass spectrometry (LV-DMI-GC-TOF-MS) for the determination of pesticides in fruit-based baby foods. Food Addit Contam 21:658–669
Gilbert-López B, García-Reyes JF, Ortega-Barrales P, Molina-Díaz A, Fernández-Alba AR (2007) Analyses of pesticide residues in fruit-based baby food by liquid chromatography/electrospray ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 21(13):2059–2071
Brera C, De Santis B, Debegnach F, Miraglia M (2008) Mycotoxins, ch. 12. In: Pico Y (ed) Food contaminants and residue analysis. Comprehensive analytical chemistry, volume 51. Elsevier, Oxford, UK
Pitt JI, Wild CP, Baan RA, Gelderblom WCA, Miller JD, Riley RT, Wu F (2012) Improving public health through mycotoxin control. International Agency for Research on Cancer Publication No 158, Geneva, Switzerland
Miller JD, Schaafsma AW, Bhatnagar D, Bondy G, Carbone I, Harris LJ, Harrison G, Munkvold GP, Oswald IP, Pestka JJ, Sharpe L, Sumarah MW, Tittlemier SA, Zhou T (2014) Mycotoxins that affect the North America agri-food sector:state of the art and directions for the future. World Mycotoxin Journal 7:63–82
Rahmani A, Jinap S, Soleimany F (2009) Qualitative and quantitative analysis of mycotoxins. Comprehensive Reviews in Food Science and Food Safety 8:202–251
European Union (2022) Methods of sampling and analysis for the control of levels of certain contaminants in foodstuffs. https://eur-lex.europa.eu/EN/legal-content/summary/methods-of-sampling-and-analysis-for-the-control-of-levels-of-certain-contaminants-in-foodstuffs.html
Siantar DP, Trucksess MW, Scott PM, Herman EM (eds) (2008) Food contaminants: mycotoxins and food allergens. ACS symposium series 1001. American Chemical Society, Washington, DC
Commission Regulation (EC) N 401/2006 of 23 February 2006. Laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in food stuff
Agriopoulou S, Stamatelopoulou E, Varzakas T (2020) Advances in Analysis and Detection of Major Mycotoxins in Foods. Foods, 9:518
Sulyok M, Berthiller F, Krska R, Schuhmacher R (2006) Development and validation of a liquid chromatography/tandem mass spectrometric method for the determination of 39 mycotoxins in wheat and maize. Rapid Commun Mass Spectrom 20:2649–2659
Tsao R, Zhou T (2000) Micellar electrokinetic capillary electrophoresis for rapid analysis of patulin in apple cider. J Agric Food Chem 48:5231–5235
Peña R, Alcaraz MC, Arce L, Ríos A, Valcárcel M (2002) Screening of aflatoxins in feed samples using a flow system coupled to capillary electrophoresis. J Chromatogr A 967:303–314
Maragos CM, Appell M (2007) Capillary electrophoresis of the mycotoxin zearalenone using cyclodextrin-enhanced fluorescence. J Chromatogr A 1143:252–257
Kos G, Sieger M, McMullin D, Zahradnik C, Sulyok M, Oner T, Mizaikoff B, Krska R (2016) A novel chemometric classification for ftir spectra of mycotoxin-contaminated maize and peanuts at regulatory limits, Food Addit Contam Part A, 33:1596–1607.
Berardo N, Pisacane V, Battilani P, Scandolara A, Pietri A, Marocco A (2005) Rapid detection of kernel rots and mycotoxin in maize by near-infrared reflectance spectroscopy. J Agric Food Chem 53:8128–8134
Pettersson H, Åberg L (2003) Near infrared spectroscopy for determination of mycotoxin in cereals. Food Control 14:229–232
De la Calle MB, Anklam E (2005) Semicarbazide: occurrence in food products and state-of-the-art in analytical methods used for its determination. Anal Bioanaly Chem 382:968–977
United States Department of Agriculture Red book archives. USDA Food Safety Inspection Service. http://www.fsis.usda.gov/wps/portal/fsis/topics/data-collection-and-reports/chemistry/red-books/archive
Chen J, Ying G, Deng W (2019) Antibiotic Residues in Food: Extraction, Analysis, and Human Health Concerns. J Agric Food Chem, 67:7569–7586
AOAC (2023) The AOAC International rapid methods validation process. http://jornades.uab.cat/workshopmrama/sites/jornades.uab.cat.workshopmrama/files/AOAC_RI.pdf
Bulthaus M (2004) Detection of Antibiotic/Drug Residues in Milk and Dairy Products, ch. 12. In: Wehr HM, Frank JF (eds) Standard methods for the examination of dairy products, 17th edn. American Public Health Association, Washington, DC
FDA (2006) Preparation and LC/MS/MS analysis of honey for fluoroquinolone residues. 29 Sept, 2006 (last updated 11/01/2017). http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm071495.htm
Roybal J, Walker CC, Pfenning AP, Turnipseed SB, Storey JM, Gonzales SA, Hurlbut JA (2002) Concurrent determination of four fluoroquinolones in catfish, shrimp, and salmon by liquid chromatography with fluorescence detection. J AOAC Int, 85:1293–1301
Holstege DM, Puschner B, Whitehead G, Galey FD (2002) Screening and mass spectral confirmation of B-lactam antibiotic residues in milk using LC-MS/MS. J Agric Food Chem 50(2):406–411
Cavalier C, Curini R, Di Corcia A, Nazzari M, Samperi R (2003) A simple and sensitive liquid chromatography-mass spectrometry confirmatory method for analyzing sulfonamide antibacterials in milk and egg. J Agric Food Chem 51:558–566
Clark SB, Turnipseed SB, Madson MR (2005) Confirmation of sulfamethazine, sulfathiazole, and sulfadimethoxine residues in condensed milk and soft-cheese products by liquid chromatography/tandem mass spectrometry. J AOAC Int 88:736–743
Wang J, Leung D (2007) Analyses of macrolide antibiotic residues I eggs, raw milk, and honey using both ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 21(19):3213–3122
Ahmed FE (2004) Testing of genetically modified organisms in foods. CRC, Boca Raton, FL
Heller KJ (2003) Genetically engineered food: methods and detection. Wiley-VCH, Weinheim, Germany
Jackson JF, Linskens HF (2009) Testing for genetic manipulation in plants (molecular methods of plant analysis). Springer, New York
FDA (2023) Food allergies: What you need to know. (Last updated 04/12/2023). https://www.fda.gov/food/buy-store-serve-safe-food/food-allergies-what-you-need-know
FDA (2004) Food allergen labeling and consumer protection act of 2004 (Public Law 108–282, Title II). (Last updated 11/29/2022). http://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/Allergens/ucm106187.htm
Prado M, Ortea I, Vial S, Rivas J, Calo-Mata P, Velázquez J (2015) Advanced DNA- and protein-based methods for the detection and investigation of food allergens. Crit Rev Food Sci Nutr. https://doi.org/10.1080/10408398.2013.873767
Westphal CD (2008) Improvement of immunoassays for the detection of food allergens, ch. 29. In: Siantar DP, Trucksess MW, Scott PM, Herman EM (eds) Food contaminants: mycotoxins and food allergens. ACS symposium series 1001, American Chemical Society, Washington, DC
Yman IM, Eriksson A, Johansson MA, Hellenas K-E (2006) Food allergen detection with biosensor immunoassays. J AOAC Int 89(3):856–861
FSIS (2023) Chemical residues and contaminants. https://www.fsis.usda.gov/science-data/data-sets-visualizations/chemical-residues-and-contaminants
FDA (2023) Food program compendium of analytical laboratory methods: Chemical analytical manual. https://www.fda.gov/food/laboratory-methods-food/foods-program-compendium-analytical-laboratory-methodsCAM
Taylor SL, Bush RK, Nordlee JA (2003) Sulfites, ch. 24. In: Metcalfe DD, Simon RA (eds) Food allergy: adverse reactions to foods and food additives, 3rd edn. Blackwell, Malden, MA, p 324–341
Bush RK, Montalbano MM (2014) Asthna and Food Additives, ch. 27. In: Metcalfe DD, Sampson HA, Simon RA, Lack G (eds) Food allergy: adverse reactions to foods and food additives, 5th edn. Wiley-Blackwell, Hoboken, NJ, p 361–374
Anonymous (2016) Code of federal regulations. Food; exemptions from labeling. 21 CFR 101.100 (a) (4). US Government Printing Office, Washington, DC
FDA (2021) Determination of sulfites in food using liquid chromatography-tandem mass spectrometry (LC-MS/MS). FDA Foods program compendium of analytical laboratory methods: Chemical analytical manual (CAM). Method C-004.03. Sept 15, 2021. https://www.fda.gov/media/114411/download
Eaton AD, Clesceri LS, Rice EW, Greenberg AE (eds) (2005) Standard methods for the examination of water and wastewater, 21st edn., Method 4500 – SO3−2 B. American Public Health Association, Washington, DC
McFeeters RF, Barish AO (2003) Sulfite analysis of fruits and vegetables by high-performance liquid chromatography (HPLC) with ultraviolet spectrophotometric detection. J Agric Food Chem 51:1513–1517
Chung SWC, Chan BTP, Chan ACM (2008) Determination of free and reversibly-bound sulfite in selected foods by high-performance liquid chromatography with fluorometric detection. J AOAC Int 91:98–102
Sindelar JJ, Miklowski AL (2011) Sodium nitrite in processed meat and poultry meats: a review of curing and examining the risk/benefit of its use. American Meat Science Association 3:1–14
Katan MB (2009) Nitrate in foods: harmful or healthy? Amer J Clin Nutr 90:11–12
Anonymous (2023) Code of federal regulations. Bottled water. 21 CFR 165.110. US Government Printing Office, Washington, DC
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Ismail, B.P., Nielsen, S.S. (2024). Analysis of Food Contaminants, Residues, and Chemical Constituents of Concern. In: Ismail, B.P., Nielsen, S.S. (eds) Nielsen's Food Analysis. Food Science Text Series. Springer, Cham. https://doi.org/10.1007/978-3-031-50643-7_33
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