Glutathione Peroxidase 1 ELISA Kit
(6 references)-
- Target See all Glutathione Peroxidase 1 (GPX1) ELISA Kits
- Glutathione Peroxidase 1 (GPX1)
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 31.25 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.25 pg/mL
- Application
- ELISA
- Purpose
- The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This kit recognizes Rat GPX1 in samples. No Significant cross-reactivity or interference between Rat GPX1 and analogues was observed.
- Sensitivity
- 18.75 pg/mL
- Components
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
- Discover our best selling GPX1 ELISA Kit
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Glutathione Peroxidase 1 (GPX1) ELISA Kit
GPX1 Reactivity: Human Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Mouse Colorimetric Sandwich ELISA 4.688 IU/mL - 300 IU/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Horse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Chicken Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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- Sample Volume
- 100 μL
- Assay Time
- 3.5 h
- Plate
- Pre-coated
- Protocol
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- Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
- Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- Reagent Preparation
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- Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 2000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 2000, 1000, 500, 250, 125, 62.5, 31.25, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 2000 pg/mL working solution to the first tube and mix up to produce a 1000 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- Sample Preparation
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- It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
- If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
- If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
- Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GPX1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GPX1 were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %. - Restrictions
- For Research Use only
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- Storage
- 4 °C,-20 °C
- Storage Comment
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- For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- Expiry Date
- 12 months
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: "Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods." in: Biological trace element research, Vol. 199, Issue 4, pp. 1456-1468, (2021) (PubMed).
: "l-theanine alleviates liver and kidney dysfunction in septic rats induced by cecal ligation and puncture." in: Life sciences, Vol. 249, pp. 117502, (2020) (PubMed).
: "The Haematological Effects of Oleanolic Acid in Streptozotocin-Induced Diabetic Rats: Effects on Selected Markers." in: Journal of diabetes research, Vol. 2019, pp. 6753541, (2020) (PubMed).
: "Neointimal hyperplasia in the inferior vena cava of adenine-induced chronic kidney disease rats with aortocaval fistulas." in: Clinical and experimental nephrology, Vol. 24, Issue 11, pp. 1007-1014, (2020) (PubMed).
: "The effects of exercise treatment on learning and memory ability, and cognitive performance in diet-induced prediabetes animals." in: Scientific reports, Vol. 10, Issue 1, pp. 15048, (2020) (PubMed).
: "Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti‑inflammatory effects, and inhibition of the p38‑MAPK mechanism." in: Molecular medicine reports, Vol. 12, Issue 6, pp. 8294-300, (2016) (PubMed).
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: "Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods." in: Biological trace element research, Vol. 199, Issue 4, pp. 1456-1468, (2021) (PubMed).
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- Target See all Glutathione Peroxidase 1 (GPX1) ELISA Kits
- Glutathione Peroxidase 1 (GPX1)
- Alternative Name
- Glutathione Peroxidase 1 (GPX1 Products)
- Synonyms
- Gpx ELISA Kit, ATGPX1 ELISA Kit, F13D4.40 ELISA Kit, F13D4_40 ELISA Kit, GLUTATHIONE PEROXIDASE 1 ELISA Kit, glutathione peroxidase 1 ELISA Kit, gshpx1 ELISA Kit, AI195024 ELISA Kit, AL033363 ELISA Kit, CGPx ELISA Kit, GPx-1 ELISA Kit, GSHPx-1 ELISA Kit, GPX1 ELISA Kit, gpx1 ELISA Kit, cb690 ELISA Kit, zgc:92737 ELISA Kit, zgc:103683 ELISA Kit, GPXD ELISA Kit, GSHPX1 ELISA Kit, GSHPx ELISA Kit, wu:fd42f12 ELISA Kit, wu:fj22d06 ELISA Kit, zgc:101700 ELISA Kit, glutathione peroxidase Gpx1 ELISA Kit, glutathione peroxidase ELISA Kit, glutathione peroxidase 1 ELISA Kit, glutathione peroxidase 1a ELISA Kit, glutathione peroxidase family protein ELISA Kit, proliferating cell nuclear antigen ELISA Kit, glutathione peroxidase 1b ELISA Kit, glutathione peroxidase 1 S homeolog ELISA Kit, gpx1 ELISA Kit, GPX1 ELISA Kit, gpx ELISA Kit, Gpx ELISA Kit, Gpx1 ELISA Kit, gpx1a ELISA Kit, POPTR_0003s12620g ELISA Kit, Pcna ELISA Kit, LBL_RS00590 ELISA Kit, btuE-1 ELISA Kit, GPX2 ELISA Kit, gpx1b ELISA Kit, gpx1.S ELISA Kit
- Pathways
- Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome
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