- He is one of the most prolific of American actors, having usually done at least 4 films a year since the 1990s. In 2002 alone, he did 9 films.
- Trejo spent much of the 1960s in California prisons--Tracy (1963-1965), San Quentin (1965-1968), Soledad (1968-1969).
- Despite being known for playing intimidating and violent characters, he is widely known as being one of the nicest and friendliest actors in Hollywood who has been described as a joy to work with by co-stars.
- He and director Robert Rodriguez are second cousins.
- He was forty-one years old when he acted in his first feature film.
- Is the only actor to appear in all three of the From Dusk Till Dawn movie series.
- His characters in the films of Robert Rodriguez are usually named after knives: Desperado (1995) (Navajas), the From Dusk Till Dawn trilogy (Razor Charlie), the Spy Kids movies (Machete), the Grindhouse (2007) trailer (Machete), Predators (2010) (Cuchillo), Machete (2010) (Machete again). Only in Once Upon a Time in Mexico (2003) (Cucuy) is this not the case.
- A scarred face, a long, thin moustache, and a muscular body with large tattoos render Trejo one of the most distinctive character actors of our time.
- He has started three restaurants bearing his name. The first one was Trejo's Tacos in 2016, followed by Trejo's Cantina and Trejo's Coffee & Donuts in 2017.
- Inducted into the Texas Film Hall of Fame on March 8, 2012, in Austin, Texas.
- Was considered for the role of Det. Rey Curtis on Law & Order (1990).
- His character's name in Heat (1995) was Trejo.
- He named his five favorite films as The Searchers (1956), Death Wish (1974), Animal Factory (2000), Dirty Harry (1971) and Once Upon a Time in the West (1968).
- Volunteers at the Villalobos Pitbull Shelter.
- Was released from Soledad CTF (Correctional Training Facility) in August 1969.
- The character of Octavio in the "King Of The Hill" cartoon was based on his likeness.
- Has two sons: Danny Boy (b. 1981) from an earlier marriage, Gilbert Trejo (b. 1988) and one daughter Danielle Trejo (b. 1990).
- He was once recognized in public by a young boy, who pointed at him and kept saying "It's El Diablo!". The boy's mother was shocked, as she believed that he was insulting the actor. Trejo then went to the mother and clarified that El Diablo was a character he played in the film The SpongeBob Movie: Sponge on the Run (2020). The mother then grabbed Trejo and said that her son never talks.
- He didn't start acting until he was thirty-three.
- Owns Trejo's Cantina at 1556 North Cahuenga Boulevard in Los Angeles. It was featured in the #6 segment of Taste of the Town (2017)(#4.7).
- His surname is pronounced TRAY-hoh.
- Attended The Scandinavian Sci-Fi, Game & Film Convention in Helsingborg, Sweden (October 2009)
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