Most of the actresses were cast when the strip club where they worked was raided by the police. The casting director showed up in front of the club as it was being shut down and offered all of them work in the film.
Katherine Victor created the Batwoman costume herself because Jerry Warren would not hire a costume designer. She made the bat insignia with a cardboard cutout, outlined it on her chest with a drawing pencil, and filled it in with black eyeliner.
DC Comics, which owns the character Batman, sued the production company, Associated Distributors Productions, for copyright infringement. Contrary to popular belief, Warren won. After the lawsuit, as the popularity of the Batman television series died down, Warren re-released the film under the title "She Was a Hippy Vampire". The Mystery Science Theater 3000 (1988) episode featuring the film used the original title, "The Wild World of Batwoman," in the home video release.
In one shot, the name of the corporation as "Ayjax", not "Ajax". The shot was added after production, to prevent Ajax from suing.
Katherine Victor claimed that on set if an actor rubbed Warren the wrong way, their lines would be cut out or given to other actors. Victor claimed "the pretty brunette who was kidnapped in the beginning of the picture was supposed to be the lead girl, but for some reason Jerry thought she was getting too big for her britches and gave all her lines to the girl in the leopard tights".