(at around 1h 15 mins) After collecting ammo in the morgue Vince's gun keeps switching between being a Glock 17 and an Beretta 92FS.
(at around 45 mins) When Gabe is held captive, the handcuffs disappear and reappear between shots.
(at around 24 mins) When J.D. and Nicole are kissing in the shower they switch places all of a sudden.
(at around 19 mins) After they first arrive on the island, they are doing the bet with the Rubik's Cube. When Bobby or Lucas looks at the watch, it says SAT on it. That is supposed to be Friday night. They are investigating the crime scene Saturday morning. They had just arrived there and are settling in.
(at around 1h 35 mins) Towards the end of the movie, Sara and Gabe are standing on the beach as the helicopter approaches. It can be seen that they are standing very close to the water. In the next shot from the opposite side, they are suddenly standing at a considerable distance from the water.
(at around 31 mins) Exposure to liquid nitrogen is relatively harmless, and the liquid nitrogen will not freeze a person's legs or body instantly. Someone's legs wouldn't be frozen and broken just after only few second exposure.
(at around 1h 35 mins) During the underwater shootout at the end of the film, the semi-automatic pistols used would not cycle the rounds as shown. The guns would fire underwater, but after the first shot the guns would jam because there would not be enough pressure generated to overcome the water pressure and fully eject the spent cartridge. A second shot would not be possible until the jam is manually cleared.
(at around 44 mins) Each set of bloody numbers is supposed to continuously repeat itself on each of the windows. However, on the window that reads 299,792,458 there is a mistake above this number the first time it shows you the window. It reads 892,792,458.
(at around 25 mins) When Bobby takes out the starter of the tube-light, the tube-light goes off. The starter only serves to start the light and makes no contribution to its continued operation.
(at around 18 mins) Liquid nitrogen is shipped/stored in dewars. Liquid nitrogen in a cylinder would cause the cylinder to burst as the nitrogen boils away.
(at around 1h 2 mins) During the DNA test sequence, the visuals on the progress meter for the "DNA sampling machine" read sample amplfication, rather than sample amplification.
(at around 3 mins) When J.D. and his partner are driving past a house, then stop and back up, a tow line is visible pulling the car backwards.
(at around 31 mins) Before the gas cylinder topples we see an industrial yellow label for - Helium.
(at around 27 mins) The CCTV camera shown in the film has no cables coming from the cable entry glands at the back of the housing, nor is the mount it is on a cable-concealed bracket, thus the camera would not be powered or capable of transmitting footage. Additionally, the housing and bracket shown is not a PTZ (Pan-Tilt-Zoom) telemetry housing and thus would not be able to rotate side to side.
(at around 13 mins) When Bobby is talking to Jen after making the bet with Lucas, he disappears and reappears as Rafe is profiling the man at the bar.
(at around 18 mins) When the team enters the main building on the island for the first time there is an overhead shot in which an entire sound crew holding a boom mic is visible on a balcony in the lower right-hand corner of the screen.
(at around 27 mins) Just as Rafe and Bobby are about to enter the restaurant, the CCTV camera is seen again and the cameraman can be seen reflected in the camera's lens.