Trivia

(At around forty-four minutes) When the male flight attendant puts the snake in the microwave, he can be seen hitting the "snake" preset button on that microwave, a somewhat unusual preset for a microwave.

Contrary to popular belief, Samuel L. Jackson's agent insisted that the title be changed, because Jackson "couldn't" work on a film with such a title. When Jackson heard about all of this, he responded with the much cited comment, "We're totally changing that back. That's the only reason I took the job. I read the title."

In March 2006, New Line Cinema, due to massive fan interest on the Internet, allowed for a five day re-shoot to film new scenes to take the movie from PG-13 to an R-rated film (originally the film wrapped principal photography in September 2005). Among these additions is the Samuel L. Jackson character's line, "I've had it with these motherfucking snakes on this motherfucking plane", a line that originated in an anticipatory Internet parody of the movie.

(At around one hour twenty-six minutes) The movie's line "I have had it with these motherfucking snakes on this motherfucking plane!" was voted as the #55 of "The 100 Greatest Movie Lines" by Premiere in 2007.

This film's title originated at an after-work happy hour among Hollywood colleagues to see who could come up with the most awful pitch for a movie. Producer Craig Berenson, who worked for DreamWorks at the time, gave his pitch for this movie based on a script called "Venom".