Simultaneous Multifocal Plane Fourier Ptychographic Microscopy Utilizing a Standard RGB Camera
Abstract
:1. Introduction
2. Methods
2.1. Formation of Wavelength-Dependent Multifocal Planes
2.2. Acquiring Multifocal Plane Images Simultaneously Using a Color Camera
3. Results
4. Transparent OLED Measurement Using Simultaneous Multifocal Plane FPM
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Zheng, G.; Horstmeyer, R.; Changhuei, Y. Wide-Field, High-Resolution Fourier Ptychographic Microscopy. Nat. Photonics 2013, 7, 739–745. [Google Scholar] [CrossRef]
- Dong, S.; Bian, Z.; Shiradkar, R.; Zheng, G. Sparsely Sampled Fourier Ptychography. Opt. Express 2014, 22, 5455–5464. [Google Scholar] [CrossRef]
- Dong, S.; Horstmeyer, R.; Shiradkar, R.; Guo, K.; Ou, X.; Bian, Z.; Xin, H.; Zheng, G. Aperture-Scanning Fourier Ptychography for 3D Refocusing and Super-Resolution Macroscopic Imaging. Opt. Express 2014, 22, 13586–13599. [Google Scholar] [CrossRef]
- Feng, T.; Wang, A.; Wang, Z.; Liao, Y.; Pan, A. Linear-space-variant model for Fourier ptychographic microscopy. Opt. Lett. 2024, 49, 2617–2620. [Google Scholar] [CrossRef]
- Tian, L.; Liu, Z.; Yeh, L.; Chen, M.; Zhong, J.; Waller, L. Computational Illumination for High-Speed in Vitro Fourier Ptychographic Microscopy. Optica 2015, 2, 904–911. [Google Scholar] [CrossRef]
- Horstmeyer, R.; Ou, X.; Zheng, G.; Willems, P.; Yang, C. Digital Pathology with Fourier Ptychography. Comput. Med. Imaging Graph. 2015, 42, 38–43. [Google Scholar] [CrossRef] [PubMed]
- Lee, H.; Chon, B.; Ahn, H. Reflective Fourier Ptychographic Microscopy Using a Parabolic Mirror. Opt. Express 2019, 27, 34382–34391. [Google Scholar] [CrossRef] [PubMed]
- Ou, X.; Zheng, G.; Yang, C. Embedded Pupil Function Recovery for Fourier Ptychographic Microscopy. Opt. Express 2014, 22, 4960–4972. [Google Scholar] [CrossRef] [PubMed]
- Zhang, S.; Zhou, G.; Zheng, C.; Li, T.; Hu, Y.; Hao, Q. Fast digital refocusing and depth of field extended Fourier ptychography microscopy. Biomed. Opt. Express 2021, 12, 5544–5558. [Google Scholar] [CrossRef] [PubMed]
- Lim, J.; Choi, G.J.; Choi, H.; Park, N. Fourier Ptychographic Microscopy for Extended Focus Range with Conjugated Optics. Opt. Lasers Eng. 2020, 129, 106080. [Google Scholar] [CrossRef]
- Tahmasbi, A.; Ram, S.; Abraham, A.V.; Chao, J.; Ward, E.S.; Ober, R. Designing the Focal Plane Spacing for Multifocal Plane Microscopy. Opt. Express 2014, 22, 16706–16721. [Google Scholar] [CrossRef] [PubMed]
- Blanchard, P.; Greenaway, A. Simultaneous Multiplane Imaging with a Distorted Diffraction Grating. Appl. Opt. 1999, 38, 6692–6699. [Google Scholar] [CrossRef] [PubMed]
- Luo, Y.; Gelsinger-Austin, P.; Watson, J.; Barbastathis, G.; Barton, J.; Kostuk, R. Laser-Induced Fluorescence Imaging of Subsurface Tissue Structures with a Volume Holographic Spatial-Spectral Imaging System. Opt. Lett. 2008, 33, 2098–2100. [Google Scholar] [CrossRef] [PubMed]
- Milgrom, B.; Konforti, N.; Golub, M.; Marom, E. Novel Approach for Extending the Depth of Field of Barcode Decoders by Using RGB Channels of Information. Opt. Express 2010, 18, 17027–17039. [Google Scholar] [CrossRef] [PubMed]
- Lipson, A.; Lipson, S.G.; Lipson, H. Optical Physics, 4th ed.; Cambridge University Press: Cambridge, UK, 2011. [Google Scholar]
- Sun, J.; Chen, Q.; Zhang, Y.; Zuo, C. Sampling Criteria for Fourier Ptychographic Microscopy in Object Space and Frequency Space. Opt. Express 2016, 24, 15765–15781. [Google Scholar] [CrossRef] [PubMed]
- Zhang, S.; Wang, A.; Xu, J.; Feng, T.; Zhou, J.; Pan, A. FPM-WSI: Fourier ptychographic whole slide imaging via feature-domain backdiffraction. Optica 2024, 11, 634–646. [Google Scholar] [CrossRef]
- Khonina, S.; Volotovskiy, S.; Dzyuba, A.; Serafimovich, P.; Popov, S.; Butt, M. Power Phase Apodization Study on Compensation Defocusing and Chromatic Aberration in the Imaging System. Electronics 2021, 10, 1327. [Google Scholar] [CrossRef]
Parameter | Value | |
---|---|---|
Aspherical surface parameter | Y radius | 25.9736 |
Conic constant | −1.001 | |
4th—order coefficient | 3.0408 × 10−6 | |
6th—order coefficient | 7.2060 × 10−10 | |
8th—order coefficient | 2.3292 × 10−14 | |
Lens thickness | 5.8 mm | |
Lens material | NBK-7 (Abbe number: 64.17, : 1.5414) | |
Numerical aperture (NA) | 0.2 | |
Clear aperture | 21.3 mm | |
Working distance | 46.2 mm | |
Effective focal length | 50.0 mm |
Parameters | Value |
---|---|
Sensor size | 6.44 mm × 4.62 mm |
Resolution | 3040 pixels × 2748 pixels |
Pixel size | 1.67 μm × 1.67 μm |
Mono/Color | Color |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Oh, G.; Choi, H. Simultaneous Multifocal Plane Fourier Ptychographic Microscopy Utilizing a Standard RGB Camera. Sensors 2024, 24, 4426. https://doi.org/10.3390/s24144426
Oh G, Choi H. Simultaneous Multifocal Plane Fourier Ptychographic Microscopy Utilizing a Standard RGB Camera. Sensors. 2024; 24(14):4426. https://doi.org/10.3390/s24144426
Chicago/Turabian StyleOh, Giseok, and Hyun Choi. 2024. "Simultaneous Multifocal Plane Fourier Ptychographic Microscopy Utilizing a Standard RGB Camera" Sensors 24, no. 14: 4426. https://doi.org/10.3390/s24144426