Protection of UVB-Induced Photoaging by Fuzhuan-Brick Tea Aqueous Extract via MAPKs/Nrf2-Mediated Down-Regulation of MMP-1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Extraction
2.2. Radical-Scavenging Activity Assays
2.3. Cell Culture, UVB-Irradiation and Cell Viability Assay
2.4. Measurement of Cellular ROS Generation
2.5. Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
2.6. Cell Lysates and Western Blotting
2.7. Statistical Analysis
3. Results
3.1. Radical Scavenging Abilities
3.2. Assay of Cell Viability in UVB-Irradiated HaCaT Cells
3.3. Effects of FBTA on ROS Generation
3.4. Effects of FBTA on Phase I and Phase II Antioxidant Enzyme Expression in HaCaT Cells
3.5. Acceleration of HO-1 Enzymes via Nrf2 Nuclear Translocation in HaCaT Cells
3.6. Effects of FBTA on the MAPK Signaling Pathway
3.7. Effects of FBTA on the Expressions of MMP-1 and Procollagen Type I
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Zhao, P.; Alam, M.B.; Lee, S.-H. Protection of UVB-Induced Photoaging by Fuzhuan-Brick Tea Aqueous Extract via MAPKs/Nrf2-Mediated Down-Regulation of MMP-1. Nutrients 2019, 11, 60. https://doi.org/10.3390/nu11010060
Zhao P, Alam MB, Lee S-H. Protection of UVB-Induced Photoaging by Fuzhuan-Brick Tea Aqueous Extract via MAPKs/Nrf2-Mediated Down-Regulation of MMP-1. Nutrients. 2019; 11(1):60. https://doi.org/10.3390/nu11010060
Chicago/Turabian StyleZhao, Peijun, Md Badrul Alam, and Sang-Han Lee. 2019. "Protection of UVB-Induced Photoaging by Fuzhuan-Brick Tea Aqueous Extract via MAPKs/Nrf2-Mediated Down-Regulation of MMP-1" Nutrients 11, no. 1: 60. https://doi.org/10.3390/nu11010060