Search Results (7)

Understanding the epidemiology and transmission dynamics of transboundary animal diseases (TADs) among wild pigs (Sus scrofa) will aid in preventing the introduction or containment of TADs among wild populations. Given the challenges associated with studying TADs in free-ranging populations, a surrogate pathogen system may predict how pathogens may circulate and be maintained within wild free-ranging swine populations, how they may spill over into domestic populations, and how management actions may impact transmission. We assessed the suitability of Torque teno sus virus 1 (TTSuV1) to serve as a surrogate pathogen for molecular epidemiological studies in wild pigs by investigating the prevalence, persistence, correlation with host health status and genetic variability at two study areas: Archbold’s Buck Island Ranch in Florida and Savannah River Site in South Carolina. We then conducted a molecular epidemiological case study within Archbold’s Buck Island Ranch site to determine how analysis of this pathogen could inform transmission dynamics of a directly transmitted virus. Prevalence was high in both study areas (40%, n = 190), and phylogenetic analyses revealed high levels of genetic variability within and between study areas. Our case study showed that pairwise host relatedness and geographic distance were highly correlated to pairwise viral genetic similarity. Molecular epidemiological analyses revealed a distinct pattern of direct transmission from pig to pig occurring within and between family groups. Our results suggest that TTSuV1 is highly suitable for molecular epidemiological analyses and will be useful for future studies of transmission dynamics in wild free-ranging pigs. Full article

Thielaviopsis paradoxa sensu lato is a soilborne fungal pathogen that causes Thielaviopsis trunk rot and heart rot in palms. The loss of structural integrity resulting from trunk rot can cause the palm trunk to collapse suddenly and poses a serious threat to life and property. Even though rudimentary knowledge about the Thielaviopsis infection process in palms is available, nothing is known about the T. paradoxa species complex in the US. The aim of this study was to characterize T. paradoxa s. lat. isolates collected from diseased palms grown in Florida. Multi-locus phylogeny using three genes, ITS, β-tubulin, and tef1-α, revealed that the isolates separate into two distinct clades with high bootstrap support. The majority of the isolates clustered with the species T. ethacetica, while two isolates formed a separate clade, distinct from T. musarum, and might represent an undescribed Thielaviopsis species. One representative isolate from each clade, when grown on three distinct media and at four different temperatures, showed differences in gross colony morphology, as well as growth rates. The T. ethacetica isolate TP5448 and the Thielaviopsis sp. isolate PLM300 grew better at opposite ends of the temperature spectrum tested in this study, i.e., 35 °C and 10 °C, respectively. In pathogenicity assays on whole plants, the T. ethacetica isolate proved to be more aggressive than Thielaviopsis sp. isolate PLM300, as it produced larger lesions when inoculated on wounded leaflets. An unequal distribution was observed for the mating-type locus of T. ethacetica, as 12 isolates carried the MAT1-1-1 allele, while the status for four isolates remained undefined. Variation in mycelial growth in response to different fungicides was also observed between the two clades. These results demonstrate the existence of two Thielaviopsis clades that can infect palms in Florida and underscore the need for targeted sampling to help uncover the diversity of Thielaviopsis species across palm-growing regions in the US. Full article

Selecting a representative optical deep-water area is crucial for accurate satellite-derived bathymetry (SDB) based on semi-theoretical and semi-empirical models. This study proposed a deep-water area selection method where potential areas were identified by integrating remote sensing imagery with existing global bathymetric data. Specifically, the effects of sun glint correction for deep-water areas on SDB estimation were investigated. The results indicated that the computed SDB had significant instabilities when different optical deep-water areas without sun glint correction were used for model training. In comparison, when sun glint correction was applied, the SDB results from different deep-water areas had greater consistency. We generated bathymetric maps for the Langhua Reef in the South China Sea and Buck Island near the U.S. Virgin Islands using Sentinel-2 multispectral images and 70% of the Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2) bathymetry data. Additionally, 30% of the ICESat-2 bathymetry data and NOAA NGS Topo-bathy Lidar data served as the validation data to evaluate the qualities of the computed SDB, respectively. The results showed that the average quality of the SDB significantly improved with sun glint correction application by a magnitude of 0.60 m in terms of the root mean square error (RMSE) for two study areas. Moreover, an evaluation of the SDB data computed from different deep-water areas showed more consistent results, with RMSEs of approximately 0.4 and 1.4 m over the Langhua Reef and Buck Island, respectively. These values were consistently below 9% of the maximum depth. In addition, the effects of the optical image selection on SDB inversion were investigated, and the SDB calculated from the images over different time periods demonstrated similar results after applying sun glint correction. The results showed that this approach for optical deep-water area selection and correction could be used for improving the SDB, particularly in challenging scenarios, thereby enhancing the accuracy and robustness of SDB. Full article

A sample of 146 Sarda bucks from eight subregions of Sardinia, Italy (Nuorese, Barbagia, Baronia, Ogliastra, Sarrabus, Guspinese, Iglesiente, Sulcis) were characterized for Y-chromosome and mtDNA markers to assess the levels of population substructure. Five polymorphic loci (SRY, AMELY, ZFY, and DDX3Y) on the Y-chromosome were genotyped. The control region of mtDNA was sequenced as a source of complementary information. Analysis of Y-chromosome data revealed the segregation of 5 haplotypes: Y1A (66.43%), Y2 (28.57%), Y1C (3.57%), Y1B1 (0.71%), and Y1B2 (0.71%). High levels of Y-chromosome diversity were observed in populations from Southwest Sardinia. The F_ST values based on Y-chromosome and mtDNA data were low, although a paternal genetic differentiation was observed when comparing the Nuorese and Barbagia populations (Central Sardinia) with the Sulcis, Iglesiente, and Sarrabus populations (Southern Sardinia). AMOVA analysis supported the lack of population substructure. These results suggest the occurrence of a historical and extensive gene flow between Sarda goat populations from different locations of Sardinia, despite the fact that this island is covered by several large mountain ranges. Introgression with foreign caprine breeds in order to improve milk production might have also contributed to avoiding the genetic differentiation amongst Sarda populations. Full article

Various industrial applications require a voltage conversion stage from DC to AC. Among them, commercial renewable energy systems (RES) need a voltage buck and/or boost stage for islanded/grid connected operation. Despite the excellent performance offered by conventional two-stage converter systems (dc–dc followed by dc–ac stages), the need for a single-stage conversion stage is attracting more interest for cost and size reduction reasons. Although voltage source inverters (VSIs) are voltage buck-only converters, single stage current source inverters (CSIs) can offer voltage boost features, although at the penalty of using a large DC-link inductor. Boost inverters are a good candidate with the demerit of complicated control strategies. The impedance source (Z-source) inverter is a high-performance competitor as it offers voltage buck/boost in addition to a reduced passive component size. Several pulse width modulation (PWM) techniques have been presented in the literature for three-phase Z-source inverters. Various common drawbacks are annotated, especially the non-linear behavior at low modulation indices and the famous trade-off between the operating range and the converter switches’ voltage stress. In this paper, a modified discontinuous PWM technique is proposed for a three-phase z-source inverter offering: (i) smooth voltage gain variation, (ii) a wide operating range, (iii) reduced voltage stress, and (iv) improved total harmonic distortion (THD). Simulation, in addition to experimental results at various operating conditions, validated the proposed PWM technique’s superior performance compared to the conventional PWM techniques. Full article

An efficient power management control for microgrids with energy storage is presented in this paper. The proposed control scheme increases the reliability and resiliency of the microgrid based on three distributed energy resources (DERs), namely Photovoltaic (PV), battery, and diesel generator with local active loads. Coordination among the DERs with energy storage is essential for microgrid management. The system model and the control strategy were developed in Real Time Digital Simulator (RTDS). Decoupled d-q current control strategy is proposed and implemented for voltage source converters (VSCs) used to interface the PV and battery sources to the AC grid. A dc-dc buck converter with a maximum power point tracking function is implemented to maximize the intermittent energy generation from the PV array. A controller is proposed and employed for both grid connected and island modes of operation. In grid connected mode, the system frequency and voltage are regulated by the grid. During a fault in island mode, the diesel generator controls the system frequency and voltage in isochronous mode. Results based on the real time digital simulator are provided to verify the superiority and effectiveness of the proposed control scheme. Full article