Search Results (2,567)

City manufacturing has once again become one of the priority areas for the sustainable development of smart cities thanks to the use of a wide range of green technologies and, first of all, additive technologies. Shortening the supply chain between producers and consumers has significant effects on economic, social, and environmental dimensions. Zoning of city multifloor manufacturing (CMFM) in areas with a compact population in large cities in the form of clusters with their own city logistics nodes (CLNs) creates favorable conditions for promptly meeting the needs of citizens for goods of everyday demand and for passenger and freight transportation. City multifloor manufacturing clusters (CMFMCs) have been already studied quite a lot for their possible uses; nevertheless, an identified research gap is related to supply chain design efficiency concerning urban CMFMCs. Thus, the main objective of this study was to explore the possibilities of lean supply chain management (LSCM) as the integrated application of lean manufacturing (LM) approaches and I4.0 technologies for customer-centric value stream management based on eliminating all types of waste, reducing the use of natural and energy resources, and continuous improvement of processes related to logistics activities. This paper presents a decision support model for LSCM in CMFMCs, which is a mathematical deterministic model. This model justifies the minimization of the number of road transport transfers within the urban area and the amount of stock that is stored in CMFMC buildings and in CLNs, and also regulating supplier lead time. The model was verified and validated using appropriately selected test data based on the case study, which was designed as a typical CMFM manufacturing system with various parameters of CMFMCs and urban freight transport frameworks. The feasibility of using the proposed model for value stream mapping (VSM) and managing logistics processes and inventories in clusters is discussed. The findings can help decisionmakers and researchers improve the planning and management of logistics processes and inventory in clusters, even in the face of unexpected disruptions. Full article

The establishment and spread of plants in their native or alien geographical ranges are determined by their germination. This study investigated the impact of different factors on variations in cardinal temperatures. We used the lm procedure and measured the effect size by the Eta-square approach to find the association of different factors (species, ecotypes, origin (native/alien), year, and life cycle) with the cardinal temperatures of 31 species. Our results showed that the base, optimum, and maximum temperatures responded differently to these factors. The base temperature was less impacted by ecotypes compared with the optimum and maximum temperatures, whereas the species had a higher impact on the variation in the base temperature. The effect of the origin of weedy plants on the base temperature was higher than the optimum and maximum temperatures. The effect of the year on the optimum temperature was more prominent than that on the base and maximum temperatures. The results confirmed that weedy alien plants preferred high and narrow ranges of base, optimum, and maximum temperatures and probably will be more problematic in summer crops. The results indicate that alien plants can benefit from warmer conditions in invaded areas at the germination stage. These findings lay the foundation for further studies to elucidate which factors are more important. Full article

Listeriosis is a rare but severe foodborne disease caused by Listeria Monocytogenes (LM), a small facultative intracellular bacillus. When occurring in pregnant women, it can be vertically transmitted to the fetus and the newborn. Infected women usually display aspecific and mild symptoms, and rarely develop the severe forms of the disease (such as neurolisteriosis). On the contrary, fetal and neonatal listeriosis can lead to complications such as fetal loss, preterm birth, neonatal sepsis, and respiratory distress syndrome (RDS). Prompt diagnosis is one of the main challenges because of the aspecific presentation of the disease; therapy relies on antibiotics that reach high intracellular concentration and can penetrate and pass the placenta reaching the fetus. Herein we report an infrequent case of LM infection involving a woman with monochorionic diamniotic twin pregnancy, followed by a comprehensive review of the available literature on listeriosis in pregnancy. Full article

Visual thinking leverages spatial mechanisms in animals for navigation and reasoning. Therefore, given the challenge of abstract mathematics and logic, spatial reasoning-based teaching strategies can be highly effective. Our previous research verified that innovative box-and-ball coloring activities help teach elementary school students complex notions like quantifiers, logical connectors, and dynamic systems. However, given the richness of the activities, correction is slow, error-prone, and demands high attention and cognitive load from the teacher. Moreover, feedback to the teacher should be immediate. Thus, we propose to provide the teacher with real-time help with LLMs. We explored various prompting techniques with and without context—Zero-Shot, Few-Shot, Chain of Thought, Visualization of Thought, Self-Consistency, logicLM, and emotional —to test GPT-4o’s visual, logical, and correction capabilities. We obtained that Visualization of Thought and Self-Consistency techniques enabled GPT-4o to correctly evaluate 90% of the logical–spatial problems that we tested. Additionally, we propose a novel prompt combining some of these techniques that achieved 100% accuracy on a testing sample, excelling in spatial problems and enhancing logical reasoning. Full article

This article presents the results of an experimental study carried out to assess the possibility of using waste wind turbine blades as retaining wall structures for slopes and trenches. The use of Vestas and LM-type blades as retaining wall components was assumed, based on ‘columns’ made of Vestas-type closed profiles filled with concrete and ‘slabs’ of fragments extracted from LM-type blades. The results of the tests and comparisons of the displacement and strain values of the components obtained using different measurement methods are presented in this paper. The force–strain and force–displacement relationships obtained from the tests were used to validate numerical models of slope protection walls and excavations designed from used wind turbine blades. According to our research, there is a high degree of variability in the strength parameters and deformation of the composite elements made from the wind turbine blades. Therefore, in the case of this type of material, characterized by a significant variation in carrying capacity, deformability, and the nature of the failures, the use of different measurement methods makes it possible to obtain much of the data necessary for assessing the reusability of wind turbine blades in building. Full article

Pleiotrophin (PTN) is a secreted factor that regulates endothelial cell migration through protein tyrosine phosphatase receptor zeta 1 (PTPRZ1) and α_vβ₃ integrin. Genetic deletion of Ptprz1 results in enhanced endothelial cell proliferation and migration, due to the decreased expression of β₃ integrin and the subsequent, enhanced cMet phosphorylation. In the present study, we investigated the effect of PTN and PTPRZ1 on activating the mTORC1 kinase and protein synthesis and identified part of the implicated signaling pathway in endothelial cells. PTN or genetic deletion of Ptprz1 activates protein synthesis in a mTORC1-dependent manner, as shown by the enhanced phosphorylation of the mTORC1-downstream targets ribosomal protein S6 kinase 1 (SK61) and 4E-binding protein 1 (4EBP1) and the upregulation of HIF-1α. The cMet tyrosine kinase inhibitor crizotinib abolishes the stimulatory effects of PTN or PTPRZ1 deletion on mTORC1 activation and protein synthesis, suggesting that mTORC1 activation is downstream of cMet. The mTORC1 inhibitor rapamycin abolishes the stimulatory effect of PTN or PTPRZ1 deletion on endothelial cell migration, suggesting that mTORC1 is involved in the PTN/PTPRZ1-dependent cell migration. The α_vβ₃ integrin blocking antibody LM609 and the peptide PTN_112–136, both known to bind to α_νβ₃ and inhibit PTN-induced endothelial cell migration, increase cMet phosphorylation and activate mTORC1, suggesting that cMet and mTORC1 activation are required but are not sufficient to stimulate cell migration. Overall, our data highlight novel aspects of the signaling pathway downstream of the PTN/PTPRZ1 axis that regulates endothelial cell functions. Full article

This study investigates the impact of strontium (Sr) additions on the corrosion resistance of an LM6 (A413) aluminium alloy. By incorporating varying concentrations of Sr (0.01 wt.% and 0.05 wt.%), the morphological and corrosion behaviours of the alloy were analysed under different corrosive environments, including sulphuric acid, sodium hydroxide, and sodium chloride solutions. The results demonstrate that Sr modifications significantly enhance the alloy’s corrosion resistance, with the most substantial improvement observed at 0.05 wt.% Sr. The analysis revealed that the weight loss of the alloy in sulphuric acid decreased by 2.5% with 0.05 wt.% Sr after 10 days of immersion, due to the formation of a stable passive oxide layer. In sodium hydroxide, however, the weight loss was reduced by 5% with 0.05 wt.% Sr after 10 days, indicating aggressive uniform corrosion. In the 3.5% sodium chloride solution, the corrosion rates remain relatively low, and the 0.05 wt.% Sr alloy showed a decrease in corrosion product formation over time, suggesting enhanced resistance. Detailed surface analyses, including 3D profiling and morphology assessments, revealed that Sr additions refine the eutectic silicon phase, transforming it from a coarse to a more desirable fibrous or lamellar structure, thus improving the alloy’s overall performance. The innovative findings underscore the potential of Sr as an effective microstructural modifier for enhancing the durability and longevity of Al-Si alloys in corrosive environments. Full article

Polyglutamine (polyQ)-mediated spinocerebellar ataxia (SCA), including SCA1, 2, 3, 6, 7, and 17, are caused by mutant genes with expanded CAG repeats, leading to the intracellular accumulation of aggregated proteins, the production of reactive oxygen species, and cell death. Among SCA, SCA3 is caused by a mutation in the ATXN3 (ataxin-3) gene. In a circumstance of polyQ aggregation, the autophagic pathway is induced to degrade the aggregated proteins, thereby suppressing downstream deleterious effects and promoting neuronal survival. In this study, we tested the effects of synthetic indole (NC009-1, -2, -3, -6) and coumarin (LM-022, -031) derivatives as chemical chaperones to assist mutant ATXN3-Q₇₅ folding, as well as autophagy inducers to clear aggregated protein. Among the tested compounds, NC009-1, -2, and -6 and LM-031 interfered with Escherichia coli-derived ATXN3-Q₇₅ aggregation in thioflavin T binding and filter trap assays. In SH-SY5Y cells expressing GFP-fused ATXN3-Q₇₅, these compounds displayed aggregation-inhibitory and neurite growth-promoting potentials compared to untreated cells. Furthermore, these compounds activated autophagy by increasing the phosphatidylethanolamine-conjugated LC3 (microtubule associated protein 1 light chain 3)-II:cytosolic LC3-I ratio in these cells. A biochemical co-immunoprecipitation assay by using a mixture of HEK 293T cell lysates containing recombinant ATXN3-Q₇₅-Venus-C-terminus (VC) or Venus-N-terminus (VN)-LC3 protein indicated that NC009-1 and -2 and LM-031 served as an autophagosome-tethering compound (ATTEC) to interact with ATXN3-Q₇₅ and LC3, and the interaction was further confirmed by bimolecular fluorescence complementation analysis in cells co-expressing both ATXN3-Q₇₅-VC and VN-LC3 proteins. The study results suggest the potential of NC009-1 and -2 and LM-031 as an ATTEC in treating SCA3 and, probably, other polyQ diseases. Full article

Background/Objectives: Sarcopenia is a muscular disease characterized by loss of muscular strength and function, affecting mainly women, and associated with increased mortality risk. The aim of this study was to compare active women with inactive women of different age groups regarding muscle mass, strength, and muscle quality. Methods: This study included 147 women (85 runners and 62 inactive), divided into <50 and ≥50 years old. Participants were evaluated for knee flexor and extensor peak torque (PT), body composition, and training habits. Results: For knee extensor muscles, there was an age group effect (F(2.146) = 40.5; p < 0.001) on absolute PT (Nm); an age group effect (F(2.146) = 44.1; p < 0.001) and a physical activity group effect (F(2.146) = 113.0; p < 0.001) on PT adjusted by body mass (Nm/kg); and an age group effect (F(2.146) = 36.9; p < 0.001) and a physical activity group effect (F(2.146) = 6.1; p = 0.014) on PT adjusted by lean mass (Nm/kgLM). There was no interaction effect. Conclusion: In both age groups, active women had greater strength and higher muscle quality than inactive women, but the difference in strength, muscle mass, and muscle quality between younger and older women were the same among runners and inactive women. Full article

L-cone opsin expression by gene therapy is a promising treatment for blue cone monochromacy (BCM) caused by congenital lack of long- and middle-wavelength-sensitive (L/M) cone function. Eight patients with BCM and confirmed pathogenic variants at the OPN1LW/OPN1MW gene cluster participated. Optical coherence tomography (OCT), chromatic perimetry, chromatic microperimetry, chromatic visual acuity (VA), and chromaticity thresholds were performed with unmodified commercial equipment and/or methods available in the public domain. Adaptive optics scanning laser ophthalmoscope (AOSLO) imaging was performed in a subset of patients. Outer retinal changes were detectable by OCT with an age-related effect on the foveal disease stage. Rod and short-wavelength-sensitive (S) cone functions were relatively retained by perimetry, although likely impacted by age-related increases in the pre-retinal absorption of short-wavelength lights. The central macula showed a large loss of red sensitivity on dark-adapted microperimetry. Chromatic VAs with high-contrast red gratings on a blue background were not detectable. Color vision was severely deficient. AOSLO imaging showed reduced total cone density with majority of the population being non-waveguiding. This study developed and evaluated specialized outcomes that will be needed for the determination of efficacy and safety in human clinical trials. Dark-adapted microperimetry with a red stimulus sampling the central macula would be a key endpoint to evaluate the light sensitivity improvements. VA changes specific to L-opsin can be measured with red gratings on a bright blue background and should also be considered as outcome measures in future interventional trials. Full article

Can bi-encoders, without additional fine-tuning, achieve a performance comparable to fine-tuned BERT models in classification tasks? To answer this question, we present a simple yet effective approach to text classification using bi-encoders without the need for fine-tuning. Our main observation is that state-of-the-art bi-encoders exhibit varying performance across different datasets. Therefore, our proposed approaches involve preparing multiple bi-encoders and, when a new dataset is provided, selecting and ensembling the most appropriate ones based on the dataset. Experimental results show that, for text classification tasks on subsets of the AG News, SMS Spam Collection, Stanford Sentiment Treebank v2, and TREC Question Classification datasets, the proposed approaches achieve performance comparable to fine-tuned BERT-Base, DistilBERT-Base, ALBERT-Base, and RoBERTa-Base. For instance, using the well-known bi-encoder model all-MiniLM-L12-v2 without additional optimization resulted in an average accuracy of 77.84%. This improved to 89.49% through the application of the proposed adaptive selection and ensemble techniques, and further increased to 91.96% when combined with the RoBERTa-Base model. We believe that this approach will be particularly useful in fields such as K-12 AI programming education, where pre-trained models are applied to small datasets without fine-tuning. Full article

Patients with unstable angina present clinical characteristics of atherosclerotic plaque vulnerability, contrary to chronic coronary syndrome patients. The process of athersclerotic plaque destabilization is also regulated by microRNA particles. In this study, the investigation on expression levels of microRNAs inhibiting the expression of proteins that protect from atherosclerotic plaque progression (miR-92a inhibiting KLF2, miR-10b inhibiting KLF4, miR-126 inhibiting MerTK, miR-98 inhibiting IL-10, miR-29b inhibiting TGFβ1) was undertaken. A number of 62 individuals were enrolled—unstable angina (UA, n = 14), chronic coronary syndrome (CCS, n = 38), and healthy volunteers (HV, n = 10). Plasma samples were taken, and microRNAs expression levels were assessed by qRT-PCR. As a result, the UA patients presented significantly increased miR-10b levels compared to CCS patients (0.097 vs. 0.058, p = 0.033). Moreover, in additional analysis when UA patients were grouped together with stable patients with significant plaque in left main or proximal left anterior descending (“UA and LM/proxLAD” group, n = 29 patients) and compared to CCS patients with atherosclerotic lesions in other regions of coronary circulation (“CCS other” group, n = 25 patients) the expression levels of both miR-10b (0.104 vs. 0.046; p = 0.0032) and miR-92a (92.64 vs. 54.74; p = 0.0129) were significantly elevated. In conclusion, the study revealed significantly increased expression levels of miR-10b and miR-92a, a regulator of endothelial protective KLF factors (KLF4 and KLF2, respectively) in patients with more vulnerable plaque phenotypes. Full article

Background/Objective: Although low-methoxy (LM) pectin (polysaccharides extracted from citrus peels) can reduce inflammation by binding to and inhibiting the TLR-2 pathway in animal models and in vitro studies, the anti-inflammatory effects of LM pectin in humans and mood have not been explored to date. The purpose of this study is to assess the role of dietary supplementation with LM pectin in healthy volunteers on inflammatory markers and on mood, specifically anxiety and depression. Methods: We carried out a 4-week dietary intervention with LM citrus pectin on healthy volunteers (N = 14, age 40 ± 16 y, BMI 24.7 ± 3.0 kg/m², sex F 57%) comparing the effects of daily supplementation with 20 g of LM citrus pectin versus 10 g of maltodextrin as the control (N = 15 age 43.2 ± 11 y, BMI 25.18 ± 2.0 kg/m², sex F 66%). The effects on mood and inflammation were also tested with LM pectin at 5 g, 10 g and 15 g (2 weeks each) in an independent cohort of n = 15 healthy volunteers (age 36 ± 21 y, BMI 23.5 ± 2.4 kg/m², sex F 80%). We assessed serum levels of TNF-alpha (downstream from TLR-2 activation), IL-1 beta, IL-6, IL-10, INF-gamma, CRP, zonulin and TLR-2 concentration which were measured using ELISA in blood samples collected at both the baseline and follow-up visits. Validated measures of anxiety and depression were collected at baseline and follow-up. Results: Supplementation with 20 g of LM pectin resulted in decreases in the pro-inflammatory markers TNF-alpha, IL-1 beta, IL-6 and INF-gamma (all p < 0.05) and an increase in anti-inflammatory marker IL-10 (p = 0.01) at the end of the 4 weeks. No such effects were observed in the control group. In addition, a significant drop in anxiety scores (from 8.38 to 4.46, p < 0.006) was found with the 20 g/day intervention but not in the control arm. In the dose–response study, anti-inflammatory effects were seen only at 15 g for TNFα (p < 0.003) and a suggestive increase in IL-10 (p = 0.08), alongside a drop in TLR-2 (p < 0.027). No significant anti-inflammatory effects were observed at 5 g and 10 g doses of LM pectin supplementation. Significant dose-dependent drops in both anxiety and depression scores were found with 10 g (p < 0.001) and 15 g per day (p < 0.0002). Conclusions: The current study identifies anxiety-reducing and anti-inflammatory effects of supplementation with 15 g/day LM pectin in healthy humans. Further research is needed to elucidate the precise mechanism and to validate the efficient dose and minimum duration of supplementation. Full article

Background/Objectives: This study investigated the anticancer potential of an aqueous extract of the fungus Fomitopsis betulina. Methods: The study assessed the effect of the extract on nine cancer cell lines, including melanoma (LM-MEL-75), lung cancer (A549), and colorectal cancer (HT29, LoVo), and four normal cell lines. The cytotoxicity of the extract was evaluated using MTT, sulforhodamine-B (SRB), and clonogenic viability assays. Additionally, the study examined the effect of the extract on plant model organisms, garden cress (Lepidium sativum) and common onion (Allium cepa), to further investigate its biological activity. Results: The assays demonstrated selective cytotoxicity of the extract toward cancer cells, while sparing normal cells. The extract induced significant cytotoxic effects at lower concentrations in lung cancer, melanoma, and colon cancer cells, showing promise as a potential anticancer agent. The results also revealed that the extract inhibited seed germination and root growth, suggesting its potential to disrupt cell cycles and induce apoptosis. Conclusions: This study highlights the therapeutic potential of F. betulina and highlights the need for further research to identify the active ingredients and mechanisms underlying its anticancer effects. Full article

Listeria monocytogenes (Lm) is responsible for listeriosis, a serious foodborne disease, with high hospitalization and mortality rates worldwide. The main cause of listeriosis in humans is the consumption of ready-to-eat (RTE) foods; Commission Regulation (EC) No 2073/2005 establishes microbiological criteria for Lm in RTE foods. Raw fish products are widely consumed, e.g., in sushi and various seafood recipes (e.g., carpaccio, sashimi, maki, nigiri, tartare, etc.), but are not subjected to RTE food safety criteria. The aim of our study was to assess the growth potential of Lm in raw sea bass fillets obtained from a leading aquaculture company in Greece. In order to assess the growth of Lm in raw sea bass fillets, we applied the “challenge test”, a scientific experiment designed to assess the growth of Lm within a specific food product under controlled conditions. According to our results, and taking into consideration the health risk for the listeriosis-vulnerable population, raw fish products utilized in the preparation of RTE foods, including sushi and an array of seafood dishes, should be incorporated in the Category of Safety Criteria of Regulation (EC) No 2073/2005 “Ready-to-eat food able to support the growth of Listeria monocytogenes”. Full article