Search Results (36,891)

The melanoma-associated antigen (MAGE) family found in eukaryotes plays a crucial role in cell proliferation and differentiation, spermatogenesis, neural development, etc. This study explored the validation and evolution of MAGE genes in eukaryotic genomes and their distribution and expression patterns in pigs. In total, 249 MAGE genes were found on 13 eukaryotic species. In total, 33, 25, and 18 genes were located on human, mouse, and pig genomes, respectively. We found eight, four, and three tandemly duplicated gene clusters on the human, mouse, and pig genomes, respectively. The majority of MAGE genes in mammals are located on the X chromosome. According to the phylogenetic analysis, the MAGE family genes were classified into 11 subfamilies. The NDN gene in zebrafish (DreNDN) was the root of this evolutionary tree. In total, 10 and 11 MAGE genes on human and mouse genomes, respectively, exhibited a collinearity relationship with the MAGE genes on pig genomes. Taking the MAGE family genes in pigs, the MAGE subfamilies had similar gene structures, protein motifs, and biochemical attributes. Using the RNA-seq data of Duroc pigs and Rongchang pigs, we detected that the expression of type I MAGE genes was higher in reproductive tissues, but type II MAGE genes were predominantly expressed in the brain tissue. These findings are a valuable resource for gaining insight into the evolution and expression of the MAGE family genes. Full article

For commercial processes, through-hole AAO membranes are fabricated from high-purity aluminum by chemical etching. However, this method has the disadvantages of using heavy-metal solutions, creating large amounts of material waste, and leading to an irregular pore structure. Through-hole porous alumina membrane fabrication has been widely investigated due to applications in filters, nanomaterial synthesis, and surface-enhanced Raman scattering. There are several means to obtain freestanding through-hole AAO membranes, but a fast, low-cost, and repetitive process to create complete, high-quality membranes has not yet been established. Here, we propose a rapid and efficient method for the multi-detachment of an AAO membrane at room temperature by integrating the one-time potentiostatic (OTP) method and two-step electrochemical polishing. Economical commercial AA1050 was used instead of traditional high-cost high-purity aluminum for AAO membrane fabrication at 25 °C. The OTP method, which is a single-step process, was applied to achieve a high-quality membrane with unimodal pore distribution and diameters between 35 and 40 nm, maintaining a high consistency over five repetitions. To repeatedly detach the AAO membrane, two-step electrochemical polishing was developed to minimize damage on the AA1050 substrate caused by membrane separation. The mechanism for creating AAO membranes using the OTP method can be divided into three major components, including the Joule heating effect, the dissolution of the barrier layer, and stress effects. The stress is attributed to two factors: bubble formation and the difference in the coefficient of thermal expansion between the AAO membrane and the Al substrate. This highly efficient AAO membrane detachment method will facilitate the rapid production and applications of AAO films. Full article

The NW-SE-trending dextral strike-slip faults on the north side of the Tian Shan, e.g., the Karatau fault, Talas–Fergana fault, Dzhalair–Naiman fault, Aktas fault, Dzhungarian fault, and Chingiz fault, play an important role in accommodating crustal shortening. The classic viewpoint is that these strike-slip faults are an adjustment product caused by the difference in the crustal shortening from west to east. Another viewpoint attributes the dextral strike-slip fault to large-scale sinistral shearing. The Alakol Lake fault is a typical dextral strike-slip fault in the north Tian Shan that has not been reported. It is situated along the northern margin of the Dzhungarian gate, stretching for roughly 150 km from Lake Ebinur to Lake Alakol. Our team utilized aerial photographs, satellite stereoimagery, and field observations to map the spatial distribution of the Alakol Lake fault. Our findings provided evidence supporting the assertion that the fault is a dextral strike-slip fault. In reference to its spatial distribution, the Lake Alakol is situated in a pull-apart basin that lies between two major dextral strike-slip fault faults: the Chingiz and Dzhungarian faults. The Alakol Lake fault serves as a connecting structure for these two faults, resulting in the formation of a mega NW-SE dextral strike-slip fault zone. According to our analysis of the dating samples taken from the alluvial fan, as well as our measurement of the displacement of the riser and gully, it appears that the Alakol Lake fault has a dextral strike-slip rate of 0.8–1.2 mm/a (closer to 1.2 mm/a). The strike-slip rate of the Alakol Lake fault is comparatively higher than that of the Chingiz fault in the northern region (~0.7 mm/a) but slower than that of the Dzhungarian fault in the southern region (3.2–5 mm/a). The Chingiz–Alakol–Dzhungarian fault zone shows a gradual decrease in deformation towards the interior of the Kazakhstan platform. Full article

Exposure to PM_2.5 pollution poses substantial health risks, with the precise quantification of exposure being fundamental to understanding the environmental inequalities therein. However, the absence of high-resolution spatiotemporal ambient population data, coupled with an insufficiency of attribute data, impedes a comprehension of the environmental inequality of exposure risks at a fine scale. Within the purview of a conceptual framework that interlinks social strata and citizenship identity with environmental inequality, this study examines the environmental inequality of PM_2.5 exposure with a focus on the city of Xi’an. Quantitative metrics of the social strata and citizenship identities of the ambient population are derived from housing price data and mobile phone big data. The fine-scale estimation of PM_2.5 concentrations is predicated on the kriging interpolation method and refined by leveraging an advanced dataset. Employing geographically weighted regression models, we examine the environmental inequality pattern at a fine spatial scale. The key findings are threefold: (1) the manifestation of environmental inequality in PM_2.5 exposure is pronounced among individuals of varying social strata and citizenship identities within our study area, Xi’an; (2) nonlocal residents situated in the northwestern precincts of Xi’an are subject to the most pronounced PM_2.5 exposure; and (3) an elevated socioeconomic status is identified as an attenuating factor, capable of averting the deleterious impacts of PM_2.5 exposure among nonlocal residents. These findings proffer substantial practical implications for the orchestration of air pollution mitigation strategies and urban planning initiatives. They suggest that addressing the wellbeing of the marginalized underprivileged cohorts, who are environmentally and politically segregated under the extant urban planning policies in China, is of critical importance. Full article

The year 2023 marks the 12th anniversary of the Great East Japan Earthquake (GEJE). Immediately after the disaster, the number of evacuees reached approximately 470,000, but by November 2022, the number had decreased to approximately 31,000. The reconstruction of housing, disposal of debris, public infrastructure development, and overall restoration and reconstruction has progressed steadily. However, a re-examination of the status of industrial restoration and reconstruction reveals that restoration and reconstruction have not progressed in some areas. This research statistically analyzes how the Japanese public perceives the issues around the recovery process and what memories and records they would like to learn from regarding the GEJE. The purpose of this study is to ask about reconstruction issues and lessons learned from the GEJE by conducting a web-based survey with 2000 respondents in Japan. The method of estimation is the use of ordinal logistic regression analysis to statistically estimate whether there are differences in recovery issues and lessons learned depending on individual attributes. The results suggest that those who are interested in, remember, and express anxiety about the recovery issues and lessons learned tend to be men, do not have children, are highly educated, and have a higher income. In sum, many of Japan’s citizens are highly interested in the reconstruction of agriculture, forestry, fisheries, housing, urban development, living environment, industry, and livelihood in the affected areas. In the future, they will play a central role in modernizing, scaling up, and integrating the agriculture, forestry, and fisheries industries, as well as in rebuilding towns and livelihoods. In the affected areas, it will be necessary to draw on the lessons learned from the GEJE and create reconstruction plans for the future, and then, policymakers will need to formulate reconstruction policies that reflect the concerns of the Japanese people. Full article

Natural and sustainable plant-based antioxidants and antimicrobials are highly desirable for improving food quality and safety. The present investigation assessed the antimicrobial and antioxidant properties of active components from Alkanna tinctoria L. (herb) roots, also known as Ratanjot root. Two methods were used to extract active components: microwave-assisted hot water (MAHW) and ethanolic extraction. MAHW extract yielded 6.29%, while the ethanol extract yielded 18.27%, suggesting superior Ratanjot root extract powder (RRP) solubility in ethanol over water. The ethanol extract showed significantly higher antioxidant activity than the MAHW extract. Gas Chromatography–Mass Spectrometry analysis revealed three major phenolic compounds: butanoic acid, 3-hydroxy-3-methyl-; arnebin 7, and diisooctyl pthalate. The color attributes (L*, a*, b*, H°ab, C*_ab) for the ethanolic and MAHW extracts revealed significant differences (p < 0.05) in all the above parameters for both types of extracts, except for yellowness (b*) and chroma (C*_ab) values. The ethanol extract exhibited antimicrobial activity against 14 foodborne bacteria, with a significantly higher inhibitory effect against Gram-positive bacteria (Listeria monocytogenes and Staphylococcus aureus) than the Gram-negative bacteria (Salmonella enterica serovar Typhimurium and Escherichia coli). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were both 25 mg/mL for the Gram-negative bacteria, while the MIC and MBC concentrations varied for Gram-positive bacteria (0.049–0.098 mg/mL and 0.098–0.195 mg/mL) and the antimicrobial effect was bactericidal. The antimicrobial activities of RRP extract remained stable under broad temperature (37–100 °C) and pH (2–6) conditions, as well as during refrigerated storage for 30 days. Application of RRP at 1% (10 mg/g) and 2.5% (25 mg/g) levels in a cooked chicken meatball model system prevented lipid oxidation and improved sensory attributes and retarded microbial growth during refrigerated (4 °C) storage for 20 days. Furthermore, the RRP extract was non-toxic when tested with sheep erythrocytes and did not inhibit the growth of probiotics, Lacticaseibacillus casei, and Lactiplantibacillus plantarum. In conclusion, the study suggests that RRP possesses excellent antimicrobial and antioxidant activities, thus making it suitable for food preservation. Full article

In this paper, we address the trajectory-/target-tracking and obstacle-avoidance problem for nonholonomic mobile robots subjected to diamond-shaped velocity constraints and predefined output performance specifications. The proposed scheme leverages the adaptive performance control to dynamically adjust the user-defined output performance specifications, ensuring compliance with input and safety constraints. A key feature of this approach is the integration of multiple constraints into a single adaptive performance function, governed by a simple adaptive law. Additionally, we introduce a robust velocity estimator with a priori-determined performance attributes to reconstruct the unmeasured trajectory/target velocity. Finally, we validate the effectiveness and robustness of the proposed control scheme, through extensive simulations and a real-world experiment. Full article

Yellow or sour passion fruit is a climacteric fruit with a high rate of respiration and ethylene production, and postharvest technology is needed to extend its shelf life. This study investigated the properties of a biocomposite film with chitosan (CH) incorporated with beeswax (BW) and graphene oxide (GO) nanoparticles for use as an edible coating to extend the shelf life of yellow passion fruit at 22 °C and 70% RH for eight days. CH films associated with BW showed lower water vapor permeability (WVP) than films with CH alone. However, adding GO to the CH + BW biopolymer matrix improved the WVP, decreased the solubility (12.8%), and increased the opacity of the film by 9% compared to those of the CH film. Fruits coated with CH + BW or CH + BW + GO exhibited a reduction in respiration rate, a slower ripening process by approximately 3 days, and a significant decrease in weight loss. This also resulted in a higher soluble solids content and increased antioxidative capacity of the pulp. The incorporation of GO into the CH + BW matrix resulted in a more pronounced delay of fruit ripening, as evidenced by the lower depigmentation of the peel at eight days, with a lightness approximately 10.7% lower at 54.92, a chroma value 16.5% lower at 49.33, a hue angle 7.2% higher at 92.56, a soluble solid (SS) content 16.7% higher at 11.32°Brix, and an acidity 31.9% higher at 4.18% compared to the control. Furthermore, the biopolymer packaging led to a higher consumer acceptance score for the fruit. Full article

Nanoporous materials, such as metal-organic frameworks (MOFs), are renowned for their high selectivity as gas adsorbents due to their specific surface area, nanoporosity, and active surface chemistry. A significant challenge for their widespread application is reduced gas uptake in wet conditions, attributed to competitive adsorption between gas and water. Recent studies of gas adsorption in wet materials have typically used small amounts of powdered porous materials (in the milligram range) within very small reactors (1–5 mL). This leaves a gap in knowledge about gas adsorption behaviors in larger reactors and with increased MOF sample sizes (to the gram scale). Additionally, there has been a notable absence of experimental research on MOFs heavily saturated with water. In this study, we aimed to fill the gaps in our understanding of gas adsorption in wet conditions by measuring CH₄ adsorption in MOFs. To do this, we used larger MOF samples (in grams) and a large-volume reactor. Our selection of commercially available MOFs, including HKUST-1, ZIF-8, MOF-303, and activated carbon, was based on their widespread application, available previous research, and differences in hydrophobicity. Using a volumetric approach, we measured high-pressure isotherms (at T = 274.15 K) to compare the moles of gas adsorbed under both dry and wet conditions across different MOFs and weights. The experimental results indicate that water decreases total CH₄ adsorption in MOFs, with a more pronounced decrease in hydrophilic MOFs compared to hydrophobic ones at lower pressures. However, hydrophilic MOFs exhibited stepped isotherms at higher pressures, suggesting water converts to hydrate, positively impacting total gas uptake. In contrast, the hydrophobic ZIF-8 did not promote hydrate formation due to particle aggregation in the presence of water, leading to a loss of surface area and surface charge. This study highlights the additional challenges associated with hydrate-MOF synergy when experiments are scaled up and larger sample sizes are used. Future studies should consider using monolith or pellet forms of MOFs to address the limitations of powdered MOFs in scale-up studies. Full article

Grasslands have been increasingly impacted by human activities, gradually becoming one of the most threatened ecosystems globally. Advanced geographic information technology and remote sensing techniques allow for a fresh perspective on studying the response of the grassland degradation index ($GDI$) to climate change. This study utilized remote sensing image data of grasslands to calculate the vegetation coverage and derive the $GDI$ for five grassland regions of China from 2001 to 2019. The results indicate that the national degradation status of grasslands remained at a level of mild degradation. The increasing trend of the $GDI$ in some regions was effectively inhibited by regional climate change, especially in the Northeastern and Northern Plain–Mountain–Hill Grassland regions, where the $GDI$ showed a continuous decreasing trend. $GDI$ was strongly correlated with atmospheric pressure, precipitation, temperature, and wind speed. In the arid northern region, the increasing precipitation and decreasing temperatures predominantly contributed to the depressed $GDI$. In the Qinghai–Tibetan Plateau Grassland region, the instability of the $GDI$ is attributed to fluctuating atmospheric pressure, with a correlation coefficient ranging from 0.5 to 0.8. Our findings underscore the importance of meteorological factors to evaluate and forecast grassland ecosystem stability. This understanding is vital for developing informed conservation and management strategies to address current and future climate challenges. Full article

Solution styrene–butadiene rubber (SSBR) is widely used to improve the properties of tire tread compounds. Tire wear particles (TWPs), which are generated on real roads as vehicles traverse, represent one of significant sources of microplastics. In this study, four SSBR compounds were prepared using two SSBRs with high styrene (STY samples) and 1,2-unit (VIN samples) contents, along with dicyclopentadiene resin. The abrasion behaviors were investigated using four different abrasion testers: cut and chip (CC), Lambourn, DIN, and laboratory abrasion tester (LAT100). The abrasion rates observed in the Lambourn and LAT100 abrasion tests were consistent with each other, but the results of CC and DIN abrasion tests differed from them. The addition of the resin improved the abrasion rate and resulted in the generation of large wear particles. The abrasion rates of STY samples in the Lambourn and LAT100 abrasion tests were lower than those of VIN samples, whereas the values in the CC and DIN abrasion tests were higher than those of VIN samples. The wear particles were predominantly larger than 1000 μm, except for the VIN sample in the DIN abrasion test. However, TWPs > 1000 μm are rarely produced on real roads. The size distributions of wear particles > 1000 μm were 74.0–99.5%, 65.9–93.4%, 7.2–95.1%, and 37.5–83.0% in the CC, Lambourn, DIN, and LAT100 abrasion tests, respectively. The size distributions of wear particles in the Lambourn and LAT100 abrasion tests were broader than those in the other tests, whereas the distributions in the CC abrasion test were narrower. The abrasion patterns and the morphologies and size distributions of wear particles generated by the four abrasion tests varied significantly, attributable to differences in the bound rubber contents, crosslink densities, and tensile properties. Full article

The Bundelkhand region of India falls under a semi-arid climate and is not typical for rice cultivation. Rice cultivation has been expanding in heavy-textured soils with limited water irrigation. The experiment was carried out with a split-plot design with main factor transplanting methods (line and random transplanting) and subfactor six nitrogenmanagement options (omissionN (ON), farmers’fertilizer practice (FFP), state fertilizer recommendation (SFR), IRRI Leaf Color Chart (LCC), Panjab Agriculture University (PAU-LCC) and Rice Crop Manager (RCM) replicated thrice on heavy-textured soil at the student’s research farm, Banda University of Agriculture & Technology, Banda during the wet season of 2020 and 2021. IRRI LCC and PAU LCC also had significantly higher growth parameters and yield attributes for augmenting better farm livelihoods. The two-year average significant increases in grain yield of puddled transplanted rice by IRRI LLC (24.8% and 9.1%), PAU LCC (26.8% and 10.8%) and RCM (20.0% and 4.9%) over FFP and SFR, respectively. The two-year mean agronomic efficiency was found to be significantly better with IRRI LCC (57.5% and 39.6%), PAU LCC (52.1% and 34.8%) and RCM (57.6% and 39.7%) compared to FFP and SFR, respectively. Similarly, Kg N uptake Kg N applied⁻¹ was significantly better with both the LCC and RCM-guided nitrogen application than FFP and SFR. Moreover, it was discovered that N management using SSNM choices reduced total GHG generation. According to our research, the farmers were applying nearly identical amounts of nitrogen, and SSNM tools allow for the efficient management of nitrogen in semi-arid regions by adjusting the timing of application and splitting. Full article

In order to reduce greenhouse gas emissions associated with power generation, the replacement of fossil fuels with renewables must be accompanied by the availability of dispatchable sources needed to balance electricity demand and production. Combined cycle (CC) power plants adopting post-combustion capture (PCC) can serve this purpose, ensuring near-zero CO₂ emissions at the stack, as well as high efficiency and load flexibility. In particular, the chemical absorption process is the most established approach for industrial-scale applications, although widespread implementation is lacking. In this study, different natural gas combined cycle (NGCC) configurations were modeled to estimate the burden of retrofitting the capture process to existing power plants on thermodynamic performance. Simulations under steady-state conditions covered the widest possible load range, depending on the gas turbine (GT) model. Attention was paid to the net power loss and net efficiency penalty attributable to PCC. The former can be mitigated by lowering the GT air–fuel ratio to increase the CO₂ concentration (X_CO2) in the exhaust, thus decreasing the regeneration energy. The latter is reduced when the topping cycle is more efficient than the bottoming cycle for a given GT load. This is likely to be the case in the less-complex heat recovery units. Full article

In this paper, we argue that under certain assumptions, Islamic theism moves in the direction of a multiverse. We present several arguments in two major categories. The first is based on the divine attribute of everlastingness: if God’s everlasting attributes are expressed in the creation and the universe has a finite past, then God created a multiverse. The second category involves perfect being theology: if some of God’s attributes express themselves in the creation, and God has every compossible perfection, then we should expect God to create a multiverse. Full article

Nosocomial infections, a prevalent issue in intensive care units due to antibiotic overuse, could potentially be addressed by metal oxide nanoparticles (NPs). However, there is still no comprehensive understanding of the impact of NPs’ size on their antibacterial efficacy. Therefore, this study provides a novel investigation into the impact of ZnO NPs’ size on bacterial growth kinetics. NPs were synthesized using a sol–gel process with monoethanolamine (MEA) and water. X-ray diffraction (XRD), transmission electron microscopy (TEM), and Raman spectroscopy confirmed their crystallization and size variations. ZnO NPs of 22, 35, and 66 nm were tested against the most common nosocomial bacteria: Escherichia coli, Pseudomonas aeruginosa (Gram-negative), and Staphylococcus aureus (Gram-positive). Evaluation of minimum inhibitory and bactericidal concentrations (MIC and MBC) revealed superior antibacterial activity in small NPs. Bacterial growth kinetics were monitored using optical absorbance, showing a reduced specific growth rate, a prolonged latency period, and an increased inhibition percentage with small NPs, indicating a slowdown in bacterial growth. Pseudomonas aeruginosa showed the lowest sensitivity to ZnO NPs, attributed to its resistance to environmental stress. Moreover, the antibacterial efficacy of paint containing 1 wt% of 22 nm ZnO NPs was evaluated, and showed activity against E. coli and S. aureus. Full article