Search Results (5,095)

Rehmannia glutinosa is an important medicinal herb; but its long-term cultivation often leads to continuous cropping problems. The underlying cause can be attributed to the accumulation of and alterations in root exudates; which interact with soil-borne pathogens; particularly Fusarium oxysporum; triggering disease outbreaks that severely affect its yield and quality. It is therefore crucial to elucidate the mechanisms by which root exudates induce F. oxysporum CCS043 outbreaks. In this study; the genome of F. oxysporum CCS043 from R. glutinosa’s rhizosphere microbiota was sequenced and assembled de novo; resulting in a 47.67 Mb genome comprising 16,423 protein-coding genes. Evolutionary analysis suggests that different F. oxysporum strains may adapt to the host rhizosphere microecosystem by acquiring varying numbers of specific genes while maintaining a constant number of core genes.The allelopathic effects of ferulic acid; verbascoside; and catalpol on F. oxysporum CCS043 were examined at the physiological and transcriptomic levels. The application of ferulic acid was observed to primarily facilitate the proliferation and growth of F. oxysporum CCS043; whereas verbascoside notably enhanced the biosynthesis of infection-related enzymes such as pectinase and cellulase. Catalpol demonstrated a moderate level of allelopathic effects in comparison to the other two. Furthermore; 10 effectors were identified by combining the genomic data. Meanwhile; it was found that among the effector-protein-coding genes; ChiC; VRDA; csn; and chitinase exhibited upregulated expression across all treatments. The expression patterns of these key genes were validated using qRT-PCR. Transient overexpression of the two effector-encoding genes in detached R. glutinosa leaves provided further confirmation that ChiC (GME8876_g) and csn (GME9251_g) are key effector proteins responsible for the induction of hypersensitive reactions in R. glutinosa leaf cells. This study provides a preliminary indication that the use of allelochemicals by F. oxysporum CCS043 can promote its own growth and proliferation and enhance infection activity. This finding offers a solid theoretical basis and data support for elucidating the fundamental causes of fungal disease outbreaks in continuous cropping of R. glutinosa and for formulating effective mitigation strategies. Full article

The early monitoring of cardiovascular biomarkers is essential for the prevention and management of some cardiovascular diseases. Here, we present a novel, compact, and highly integrated skin electrode as a mechanical–electrochemical dual-model E-skin, designed for the real-time monitoring of heart rate and sweat ion concentration, two critical parameters for assessing cardiovascular health. As a pressure sensor, this E-skin is suitable for accurate heart rate monitoring, as it exhibits high sensitivity (25.2 pF·kPa⁻¹), a low detection limit of 6 Pa, and a rapid response time of ~20 ms, which is attributed to the iontronic sensing interface between the skin and the electrode. Additionally, the electrode functions as a potassium ion-selective electrode based on chemical doping, achieving an enhanced response of 11 mV·mM⁻¹. A test based on the real-time monitoring of a subject riding an indoor bike demonstrated the device’s capability to monitor heart rate and sweat potassium ion levels reliably and accurately. This advancement in wearable technology offers significant potential for enhancing patient care based on the early detection and proactive management of cardiovascular conditions. Full article

Rice (Oryza sativa L.) is one of the most crucial cereal crops worldwide, serving as a staple food for a significant portion of the global population. Rice is the second most important staple food crop in Pakistan after wheat, and it is also a major export commodity. Concerning this, the current study aimed to evaluate the effects of different seed rates on the yield and yield-contributing parameters of rice varieties. The experiment was conducted over two consecutive kharif summer seasons, from 2020–21 and 2021–22, at the Pakistan Agricultural Research Council (PARC) Rice Program experimental area in Kala Shah Kaku, Lahore, Pakistan, by following a factorial randomized complete block design with three replications using coarse rice (KSK-133) and fine rice (Super Basmati) varieties. Different seed rates, including 27 kg/ha, 22 kg/ha, 17 kg/ha, and 12 kg/ha, were tested during the experiment. Different growth and yield-related attributes, such as plant height (cm), the number of productive tillers per plant, panicle length (cm), the number of grains per panicle, and grain yield (m⁻²), were recorded. The results showed that for KSK-133 and Super Basmati, the maximum grain yield was achieved at a sowing rate of 27 kg/ha in direct seed rice (DSR). The lowest yield was observed at a seeding rate of 12 kg/ha for KSK-133 and Super Basmati in DSR. Both basmati (Super Basmati) and coarse-grain (KSK-133) varieties exhibited similar responses to seed rate treatments, with the optimal performance observed at the highest seed rate of 27 kg/ha for both seasons. Grains per panicle and thousand grain weight emerged as critical determinants of yield, highlighting the need to balance vegetative growth with reproductive development. Breeding programs should focus on developing varieties that balance vegetative traits like tiller production and panicle length with reproductive traits to enhance overall yield. Based on these findings, it is concluded that using an optimal seeding rate of 27 kg/ha for direct-seeded fine and coarse rice varieties is beneficial in terms of tillers and higher yield. Full article

Risk communication from the perspective of Extreme Event Attribution (EEA), which assesses the extent to which climate change influences various extreme weather events, has significant potential for climate change communication due to its ability to make the phenomenon more relatable to citizens. This study examines the digital conversation generated following the wave of wildfires in Spain in 2022, which was declared the worst year of the 21st century in terms of hectares burned. By using the Social Network Analysis (SNA) methodology, 145,081 tweets were analyzed to construct a mention network, capturing the digital clusters formed around this discussion and highlighting the predominant tones in the debate. The findings reveal that the conversation predominantly adopted a tone of outrage and assigned responsibility. This research study offers a renewed perspective on risk communication, highlighting significant challenges faced by environmental activism on social media and underscoring the need to improve communication strategies to increase awareness and mobilization around climate change. Full article

Background: Ankylosing spondylitis (AS) is a chronic inflammatory and autoimmune disease that primarily affects the sacroiliac joints and axial skeleton. While the exact pathogenetic mechanism of AS remains unclear, previous reports have highlighted the involvement of genetic factors, immune responses, and gut microbiota dysregulation in the development of this condition. Short-chain fatty acids (SCFAs), which are microbial fermentation products derived from sugar, protein, and dietary fibers, play a role in maintaining the intestinal barrier function and reducing inflammatory responses. The aim of this study was to investigate the therapeutic potential of butyric acid (BA), an important SCFA, in the treatment of AS. Methods: To evaluate the anti-inflammatory and anti-bone loss effects of BA, a murine AS model was established using proteoglycan and dimethyl dioctadecyl ammonium (DDA) adjuvants. Various techniques, including an enzyme-linked immunosorbent assay (ELISA), magnetic resonance imaging (MRI), micro-CT, histology, quantitative PCR (qPCR) for intestinal tight junction protein expression, and 16S rDNA sequencing to analyze gut microbiota abundance, were employed to assess the inflammation and bone health in the target tissues. Results: The results indicated that BA demonstrated potential in alleviating the inflammatory response in the peripheral joints and the axial spine affected by AS, as evidenced by the reductions in inflammatory infiltration, synovial hyperplasia, and endplate erosion. Furthermore, BA was found to impact the intestinal barrier function positively. Notably, BA was associated with the downregulation of harmful inflammatory factors and the reversal of bone loss, suggesting its protective effects against AS. Conclusions: These beneficial effects were attributed to the modulation of gut microbiota, anti-inflammatory properties, and the maintenance of skeletal metabolic homeostasis. This study contributes new evidence supporting the relationship between gut microbiota and bone health. Full article

The proteinogenic amino acid proline plays crucial roles in both plant development and stress responses, far exceeding its role in protein synthesis. However, the molecular mechanisms and the relative importance of these additional functions of proline remain under study. It is well documented that both stress responses and developmental processes are associated with proline accumulation. Under stress conditions, proline is believed to confer stress tolerance, while under physiological conditions, it assists in developmental processes, particularly during the reproductive phase. Due to proline’s properties as a compatible osmolyte and potential reactive oxygen species (ROS) scavenger, most of its beneficial effects have historically been attributed to the physicochemical consequences of its accumulation in plants. However, emerging evidence points to proline metabolism as the primary driver of these beneficial effects. Recent reports have shown that proline metabolism, in addition to supporting reproductive development, can modulate root meristem size by controlling ROS accumulation and distribution in the root meristem. The dynamic interplay between proline and ROS highlights a sophisticated regulatory network essential for plant resilience and survival. This fine-tuning mechanism, enabled by the pro-oxidant and antioxidant properties of compartmentalized proline metabolism, can modulate redox balance and ROS homeostasis, potentially explaining many of the multiple roles attributed to proline. This review uniquely integrates recent findings on the dual role of proline in both ROS scavenging and signaling, provides an updated overview of the most recent research published to date, and proposes a unified mechanism that could account for many of the multiple roles assigned to proline in plant development and stress defense. By focusing on the interplay between proline and ROS, we aim to provide a comprehensive understanding of this proposed mechanism and highlight the potential applications in improving crop resilience to environmental stress. Additionally, we address current gaps in understanding and suggest future research directions to further elucidate the complex roles of proline in plant biology. Full article

Natural gas hydrates, a promising clean energy resource, hold substantial potential. Porosity plays a crucial role in hydrate systems by influencing formation processes and physical properties. To clarify the effects of porosity on hydrate elasticity, we examined methane hydrate formation and its acoustic characteristics. Experiments were conducted on sediment samples with porosities of 23%, 32%, and 37%. P- and S-wave velocities were measured to assess acoustic responses. Results show that as hydrate saturation increases, sample acoustic velocity also rises. However, high-porosity samples consistently exhibit lower acoustic velocities compared to low-porosity samples and reach a lower maximum hydrate saturation. This behavior is attributed to rapid pore filling in high-porosity samples, which blocks flow pathways and limits further hydrate formation. In contrast, hydrate formation in low-porosity sediments progresses more gradually, maintaining clearer pore channels and resulting in relatively higher hydrate saturation. Higher porosity also accelerates the shift of hydrates from cementing to load-bearing morphologies. These findings underscore porosity’s significant influence on hydrate formation and provide insights into observed variations in hydrate saturation and acoustic velocity across different experimental conditions. Full article

Glioblastoma (GBM) is the most common and lethal intracranial tumor in adults. Despite advances in the understanding of the molecular events responsible for disease development and progression, survival rates and mortality statistics for GBM patients have been virtually unchanged for decades and chemotherapeutic drugs used to treat GBM are limited. Arsenic derivatives, known as highly effective anticancer agents for leukemia therapy, has been demonstrated to exhibit cytocidal effects toward GBM cells by inducing cell death, cell cycle arrest, inhibition of migration/invasion, and angiogenesis. Differentiation induction of glioma stem-like cells (GSCs) and inhibition of neurosphere formation have also been attributed to the cytotoxicity of arsenic derivatives. Intriguingly, similar cytotoxic effects against GBM cells and GSCs have also been observed in natural agents such as anthocyanidins, tetrandrine, and bufadienolides. In the current review, we highlight the available data on the molecular mechanisms underlying the multifaceted anticancer activity of arsenic compounds and natural agents against cancer cells, especially focusing on GBM cells and GCSs. We also outline possible strategies for developing anticancer therapy by combining natural agents and arsenic compounds, as well as temozolomide, an alkylating agent used to treat GBM, in terms of improvement of chemotherapy sensitivity and minimization of side effects. Full article

In the analytical quality by design (AQbD) framework, the design of experiments (DOE) plays a very important role, as it provides information about how experimental input variables influence critical method attributes. Based on the information obtained from the DOE, mathematical models are generated and used to build the method operable design region (MODR), which is a robust region of operability. Data treatment steps are usually carried out in software such as Fusion QbD, Minitab, or StaEase 360, among others. Although there are many studies in the literature that use the DOE, none of them address important aspects of data treatment for optimization and MODR generation and compare different software calculations. The purpose of this study is to contribute to a better understanding of data treatment aspects that are frequently misread or not fully understood, such as model selection, ANOVA results, and residual analysis. The discussion will be guided by the separation of curcuminoids, using ultra-high performance liquid chromatography and eight quality attributes as responses. This study highlights the importance of carefully selecting and evaluating models, as they significantly influence the generation of the MODR. Moreover, the findings emphasize that it is essential to incorporate uncertainties into the contour plots to accurately determine the MODR in compliance with the ICH Q14 guidelines and USP General Chapter <1220>. Full article

Background/Objectives: SARS-CoV-2 was responsible for the global pandemic. Approximately 10–15% of patients with COVID-19 developed respiratory failure with adult acute respiratory distress syndrome (ARDS), which required treatment in the Intensive Care Unit (ICU). The cytokine storm observed in severe COVID-19 was frequently handled with steroids. Synergically, tocilizumab, an anti-interleukin-6 receptor monoclonal antibody, gained popularity as a cytokine storm-suppressing agent. However, immunosuppression was proven to increase the predisposition to infections with resistant bacteria. Our study aimed to assess the relationship between positive tests for secondary infections and the survival of patients with severe COVID-19-attributed ARDS treated with immunosuppressive agents. Methods: This study included 342 patients qualified for the ICU and mechanical ventilation (MV). The patients were divided based on the type of immunomodulating therapy and the culture tests results. Results: The results showed the highest survival rate among patients <61 years, favoring the combined treatment (tocilizumab + steroids). Atrial fibrillation (AF) and coronary heart disease (CHD) correlated with a lower survival rate than other comorbidities. Tocilizumab was associated with an increased risk of positive pathogen cultures, which could potentially cause secondary infections; however, the survival rate among these patients was higher. Conclusions: MV and ICU procedures as well as the application of tocilizumab significantly decreased the mortality rate in patients with severe COVID-19-related ARDS. The suppression of cytokine storms played a crucial role in survival. Tocilizumab was found to be both efficient and safe despite the ‘side effect’ of the increased risk of positive results for secondary infections. Full article

Herpes simplex virus (HSV) in humans and pseudorabies virus (PRV) in pigs are both alphaherpesviruses. Plasmacytoid dendritic cells (pDCs) make part of the peripheral blood mononuclear cells (PBMCs) and are specialized in producing large amounts of antiviral type I interferon (IFN-I). IFN-I production by PBMCs in response to both HSV-1 and PRV can be virtually exclusively attributed to pDCs. Recently, we discovered that cells infected with gEnull PRV trigger increased production of IFNalpha by porcine PBMCs/pDCs compared with cells infected with wild-type (WT) PRV. This increased IFNalpha response correlates with increased extracellular virus production triggered by gEnull PRV compared with WT PRV. The gE protein and some of its currently described functions are conserved in different alphaherpesviruses, including PRV and HSV-1. In the current study, we report that cells infected with gEnull HSV-1 trigger increased IFNalpha production by human PBMCs and increased extracellular virus production compared with WT HSV-1. Hence, these recently described functions of PRV gE are conserved in HSV-1 gE. Since the increased extracellular virus production and IFNalpha response have also been reported for successful (gEnull) PRV vaccines, the current findings may have important consequences for the rational design of HSV vaccines. Full article

MicroRNA (miR: small noncoding RNA)-150 is evolutionarily conserved and is downregulated in patients with diverse forms of heart failure (HF) and in multiple mouse models of HF. Moreover, miR-150 is markedly correlated with the outcome of patients with HF. We previously reported that systemic or cardiomyocyte-derived miR-150 in mice elicited myocardial protection through the inhibition of cardiomyocyte death, without affecting neovascularization and T cell infiltration. Our mechanistic studies also showed that the protective roles of miR-150 in ischemic mouse hearts and human cardiac fibroblasts were, in part, attributed to the inhibition of fibroblast activation via the repression of multiple profibrotic genes. However, the extent to which miR-150 expression in adult myofibroblasts (MFs) modulates the response to myocardial infarction (MI) remains unknown. Here, we develop a novel 4-hydroxytamoxifen-inducible MF-specific miR-150 conditional knockout mouse model and demonstrate that the mouse line exhibits worse cardiac dysfunction after MI. Our studies further reveal that miR-150 ablation selectively in adult MFs exacerbates cardiac damage and apoptosis after chronic MI. Lastly, MF-specific miR-150 deletion in adult mice promotes the expression of proinflammatory and profibrotic genes as well as cardiac fibrosis following chronic MI. Our findings indicate a key protective role for MF-derived miR-150 in modulating post-MI responses. Full article

Background/Objectives: This mixed-methods, cross-sectional study explored adolescent understandings of kindness, and interconnections amongst Theory of Mind (ToM; ability to attribute mental states to oneself and others), kindness, compassion, and social-psychological well-being components in 318 participants aged 10–18 (Mage = 14.58, SD = 2.31). Methods: Participants completed a battery of self-report measures and wrote responses to open-ended questions about kindness in different relational and situational contexts. Results: Most adolescents gave other-oriented, psychological definitions of kindness, increasing in detail with age. Content analysis revealed main themes of helping, followed by proactive support and respect, and differed according to the identity of the recipient (stranger, self, others), and situational context (home, school). Results showed significant positive correlations between perceptions of kindness and social (but not psychological) well-being, with gender and age differences. High levels of ToM related to high levels of perceived compassion and kindness for others, and the relations strengthened with age. Conclusions: The results highlight implications for future research on adolescents’ perceptions of prosociality, and kindness-based mental health interventions that promote social cognition and prosocial acts. Full article

Groundwater contamination with sub-lethal dissolved contaminants poses significant health risks globally, especially in rural India, where access to safe drinking water remains a critical challenge. This study explores the hydrogeochemical characterization and associated health risks of groundwater from shallow aquifers in the Marginal Ganga Alluvial Plain (MGAP) of northern India. The groundwater chemistry is dominated by Ca-Mg-CO₃ and Ca-Mg-Cl types, where there is dominance of silicate weathering and the ion-exchange processes are responsible for this solute composition in the groundwater. All the ionic species are within the permissible limits of the World Health Organization, except fluoride (F⁻) and nitrate (NO₃⁻). Geochemical analysis using bivariate relationships and saturation plots attributes the occurrence of F⁻ to geogenic sources, primarily the chemical weathering of granite-granodiorite, while NO₃⁻ contaminants are linked to anthropogenic inputs, such as nitrogen-rich fertilizers, in the absence of a large-scale urban environment. Multivariate statistical analyses, including hierarchical cluster analysis and factor analysis, confirm the predominance of geogenic controls, with NO₃⁻-enriched samples derived from anthropogenic factors. The spatial distribution and probability predictions of F⁻ and NO₃⁻ were generated using a non-parametric co-kriging technique approach, aiding in the delineation of contamination hotspots. The integration of the USEPA human health risk assessment methodology with the urbanization index has revealed critical findings, identifying approximately 23% of the study area as being at high risk. This comprehensive approach, which synergizes geospatial analysis and statistical methods, proves to be highly effective in delineating priority zones for health intervention. The results highlight the pressing need for targeted mitigation measures and the implementation of sustainable groundwater management practices at regional, national, and global levels. Full article

Aortic aneurysm, the pathological dilatation of the aorta at distinct locations, can be attributed to many different genetic and environmental factors. The resulting pathobiological disturbances generate a complex interplay of processes affecting cells and extracellular molecules of the tunica interna, media and externa. In short, aortic aneurysm can affect processes involving the extracellular matrix, lipid trafficking/atherosclerosis, vascular smooth muscle cells, inflammation, platelets and intraluminal thrombus formation, as well as various endothelial functions. Many of these processes are interconnected, potentiating one another. Newer discoveries, including the involvement of odorant olfactory receptors in these processes, have further shed light on disease initiation and pathology. Olfactory receptors are a varied group of G protein coupled-receptors responsible for the recognition of chemosensory information. Although they comprise many different subgroups, some of which are not well-characterized or identified in humans, odorant olfactory receptors, in particular, are most commonly associated with recognition of olfactory information. They can also be ectopically localized and thus carry out additional functions relevant to the tissue in which they are identified. It is thus the purpose of this narrative review to summarize and present pathobiological processes relevant to the initiation and propagation of aortic aneurysm, while also incorporating evidence associating these ectopically functioning odorant olfactory receptors with the overall pathology. Full article