Search Results (3,985)

Voltage-dependent anion channels (VDACs) are important proteins of the outer mitochondrial membrane (OMM). Their beta-barrel structure allows for efficient metabolite exchange between the cytosol and mitochondria. VDACs have further been implicated in the control of regulated cell death. Historically, VDACs have been pictured as part of the mitochondrial permeability transition pore (MPTP). New concepts of regulated cell death involving VDACs include its oligomerisation to form a large pore complex in the OMM; however, alternative VDAC localisation to the plasma membrane has been suggested in the literature and will be discussed regarding its potential role during cell death. Very recently, a phospholipid scramblase activity has been attributed to VDAC dimers, which explains the manifold lipidomic changes observed in VDAC-deficient yeast strains. In this review, I highlight the recent advances regarding VDAC’s phospholipid scramblase function and discuss how this new insight sheds new light on VDAC’s implication in regulated cell death, autophagy, and ageing. Full article

The impact of various modification methods on enhancing the adsorption performance of coal gangue (CG) for hazardous heavy metals has not been thoroughly investigated. In this study, three CG samples were first modified by calcination, followed by acid washing, alkali washing, and hydrothermal treatment, to obtain modified CG samples. The adsorption performance was assessed based on the adsorption capacities for Cd²⁺ and Pb²⁺ (i.e., q_e,Cd and q_e,Pb), and the kinetics of the adsorption processes were analyzed using kinetic equations. XRD, SEM-EDX, FTIR, and N₂ adsorption–desorption isotherms were used to elucidate the adsorption mechanisms. Results indicated that q_e,Cd and q_e,Pb of raw CG samples were approximately 10 and 25 mg/g, respectively, with only slight changes observed after calcination, acid washing, and alkali washing. In contrast, hydrothermal treatment yielded NaP and NaA zeolites, which significantly enhanced q_e,Cd and q_e,Pb to values of 48.5‒72.7 and 214.9‒247.5 mg/g, respectively. The hydrothermally treated CG samples primarily adsorbed Cd²⁺ and Pb²⁺ through ion exchange with Na⁺ within the zeolite structure, facilitating the entry of these ions into the zeolite’s pore channels. The adsorption processes were effectively described by the pseudo-second-order kinetic model. By optimizing the conditions of hydrothermal modification, the adsorption performance of CG samples is anticipated to further improve due to the creation of additional adsorption sites. Full article

Background: Recent evidence in systems neuroscience suggests that lighting conditions affect the whole chain of brain processing, from retina to high-level cortical networks, for perceptual and cognitive function. Here, visual adaptation levels to three different environmental lighting conditions, (1) darkness, (2) daylight, and (3) prolonged exposure to very bright light akin to sunlight, were simulated in lab to investigate the effects of light adaptation levels on classic cases of subjective contrast, assimilation, and contrast-induced relative depth in achromatic, i.e., ON–OFF pathway mediated visual configurations. Methods: After adaptation/exposure to a given lighting condition, configurations were shown in grouped and ungrouped conditions in random order to healthy young humans in computer-controlled two-alternative forced-choice procedures that consisted of deciding, as quickly as possible, which of two background patterns in a given configuration of achromatic contrast appeared lighter, or which of two foreground patterns appeared to stand out in front, as if it were nearer to the observer. Results: We found a statistically significant effect of the adaptation levels on the consciously perceived subjective contrast (F(2,23) = 20.73; p < 0.001) and the relative depth (F(2,23) = 12.67; p < 0.001), a statistically significant interaction between the adaptation levels and the grouping factor (F(2,23) = 4.73; p < 0.05) on subjective contrast, and a statistically significant effect of the grouping factor on the relative depth (F(2,23) = 13.71; p < 0.01). Conclusions: Visual adaption to different lighting conditions significantly alters the conscious perception of contrast and assimilation, classically linked to non-linear functional synergies between ON and OFF processing channels in the visual brain, and modulates the repeatedly demonstrated effectiveness of luminance contrast as a depth cue; the physically brighter pattern regions in the configurations are no longer consistently perceived as nearer to a conscious observer under daylight and extreme bright light adapted (rod-saturated) conditions. Full article

The Microwave Radiation Imager (MWRI) onboard the FengYun satellite plays a crucial role in global change monitoring and numerical weather prediction. Estimating and correcting geolocation errors are important to retrieving accurate geophysical variables. However, the instantaneous field of view (IFOV) inter-channel deviation, which is mainly caused by the structure mounting error and measurement error of feedhorns, is less studied. In this present study, we constructed a general theoretical model to automatically estimate the IFOV inter-channel deviations suitable for conical-scanning instruments. The model can automatically detect the along-track and across-track vectors that pass through the land–sea boundary points and are perpendicular to the actual coastlines. Regarding the midpoints of the vectors as the brightness temperature (Tb) inflection points, the IFOV inter-channel deviation is the pixel offset or distance of the maximum gradients of the Tb near the inflection points for each channel relative to the 89-GHz V-pol channel. We tested the model’s operational performance using the FY-3G/MWRI-Rainfall Mission (MWRI-RM) observations. Considering that parameter uploading adjusted the IFOV inter-channel deviations, the model’s validity was verified by comparing the adjustments calculated by the model with the theoretical changes caused by parameter uploading. The result shows that the differences between them for all window channels are less than 100 m, indicating the model’s effectiveness in evaluating the IFOV inter-channel deviation for the MWRI-RM. Furthermore, the estimated on-orbit IFOV inter-channel deviations for the MWRI-RM show that all channel deviations are less than 1 km, meeting the instrument’s design requirement of 2 km. We believe this study will provide a foundation for IFOV inter-channel registration of passive microwave payloads and spatial matching of multiple payloads. Full article

The precipitation gradient (PG) is a crucial parameter for watershed hydrological models. Analysis of daily precipitation and elevation data from 30 stations in the southeastern Tibetan Plateau (SETP) during the active phase of the Indian monsoon reveals distinct patterns. Below 3000 m, precipitation generally decreases with increasing altitude. Between 3000 and 4000 m, precipitation patterns are more complex; in western regions, precipitation increases with elevation, whereas in eastern regions, it decreases. Above 4000 m, up to the highest observation point of 4841 m, precipitation continues to decrease with elevation, with a more pronounced decline beyond a critical height. In the SETP, PGs for LYR and NYR are positive, at 11.3 ± 2.7 mm/100 m and 17.3 ± 3.8 mm/100 m, respectively. Conversely, PLZB exhibits a negative PG of −22.3 ± 4.2 mm/100 m. The Yarlung Zangbo River (YLZBR) water vapor channel plays a significant role in these PGs, with the direction and flux of water vapor potentially influencing both the direction and magnitude of the PG. Additional factors such as precipitation intensity, the number of precipitation days, precipitation frequency, and station selection also significantly impact the PG. Notable correlations between elevation and variables such as the number of precipitation days, non-precipitation days, and precipitation intensity. The precipitation intensity gradients (PIGs) are 0.06 ± 0.02 mm/d/100 m, 0.11 ± 0.04 mm/d/100 m, and −0.18 ± 0.04 mm/d/100 m for the three catchments, respectively. Future research should incorporate remote sensing data and expand site networks, particularly in regions above 5000 m, to enhance the accuracy of precipitation–elevation relationship assessments, providing more reliable data for water resource simulation and disaster warning. Full article

The effect of wall deformations on wall turbulence is a topic of fundamental importance for the advancement of flow control strategies aimed at reducing the frictional drag. Dynamic wall deformations in the form of wall-normal of in-plane oscillations represents an area that is yet to be fully explored, and that can open a new realm of drag control strategies, as well as provide fresh insights into the structure of wall turbulence. In this study, we present several results from direct numerical simulations aimed at understanding the response of wall turbulence to standing wave-like wall motion, arranged in a checkerboard pattern. More specifically, here we target a low Reynolds number turbulent channel flow, featuring standing wave-like dynamic wall deformations on both bottom and top walls, with parameterizations in terms of the frequency of oscillations, roughness height, and spacing. We quantify the effect that this type of wall motion can have on the skin friction drag, various turbulent statistics, and turbulent flow structures. In addition, taking advantage of the periodicity and the symmetry of the flow, an improved phase-averaging procedure is introduced, which enhances the smoothness of the data. The results show that this type of dynamic wall deflection can have a significant effect on the turbulent flow in proximity to the wall, and that the variation of the spatial wavenumbers of the wall deflections can make a big difference. A slight total drag reduction, in the order of 2%, was observed for some combinations of wavenumbers and frequencies, especially for the highest streamwise wavenumber case. Full article

This study aimed to evaluate the emergence, early growth, and anatomy of Schinus terebinthifolia Raddi cultivated in iron mining tailings. The seeds were obtained from trees used in urban afforestation and cultivated on two substrates: sand and iron mining tailings. The chemical composition of the mining tailing was characterized. The experiment was conducted in a growth room for 60 days. The emergence rate, seedling survival, height, number of leaves, chlorophyll content, and leaf and root anatomy were evaluated. The analysis of the composition of the mining tailings indicated that macro- and micronutrients were present, as well as potentially toxic elements such as Al, Cd, Cr, and Pb. The mining tailings reduced the emergence rate, and 25% of the seedlings died in this substrate. In addition, the mining tailings promoted a significant reduction in all parameters investigated, including seedling height, number of leaves, chlorophyll content, total leaf thickness, abaxial and adaxial epidermis thickness, palisade parenchyma thickness, and the length and width of the seeds. Additionally, the chloroplasts, the metaxylem vessel diameter, and the phloem proportion were evaluated. Interestingly, the tailings promoted an increase in the secretory channel. In the roots, no significant changes were observed in the parameters analyzed. Thus, the seeds of S. terebinthifolia germinated in the iron mining tailings, and 75% of the seedlings survived, showing their potential for reforestation. Nonetheless, iron mining tailings exhibited toxicity to S. terebinthifolia seedlings, reducing their photosynthetic tissues and, consequently, their growth; this toxicity is likely related to potentially toxic elements present in tailings. Full article

This purpose of this research is to understand the role of networked narratives in social media in modulating viewer prejudice toward ethnic neighborhoods. We designed experimental videos on YouTube based on intergroup contact theory and narrative frameworks aimed at (1) gaining knowledge, (2) reducing anxiety, and (3) fostering empathy. Despite consistent storytelling across the videos, we observed significant variations in viewer emotions, especially in replies to comments. We hypothesized that these discrepancies could be explained by the influence of the surrounding digital network on the narrative’s reception. Two-stage research was conducted to understand this phenomenon. First, automated emotion analysis on user comments was conducted to identify the varying emotions. Then, we explored contextual factors surrounding each video on YouTube, focusing on algorithmic curation inferred from traffic sources, region, and search keywords. Findings revealed that negative algorithmic curation and user interactivity result in overall negative viewer emotion, largely driven by video placement and recommendations. However, videos with higher traffic originating from viewers who had watched the storyteller’s other videos result in more positive sentiments and longer visits. This suggests that consistent exposure within the channel can foster more positive acceptance of cultural outgroups by building trust and reducing anxiety. There is the need, then, for storytellers to curate discussions to mitigate prejudice in digital contexts. Full article

Type-A γ-aminobutyric acid (GABA_A) receptors are channel proteins crucial to mediating neuronal balance in the central nervous system (CNS). The structure of GABA_A receptors allows for multiple binding sites and is key to drug development. Yet the formation mechanism of the receptor’s distinctive pentameric structure is still unknown. This study aims to investigate the role of three predominant subunits of the human GABA_A receptor in the formation of protein pentamers. Through purifying and refolding the protein fragments of the GABA_A receptor α1, β2, and γ2 subunits, the particle structures were visualised with negative staining electron microscopy (EM). To aid the analysis, AlphaFold2 was used to compare the structures. Results show that α1 and β2 subunit fragments successfully formed homo-oligomers, particularly homopentameric structures, while the predominant heteropentameric GABA_A receptor was also replicated through the combination of the three subunits. However, homopentameric structures were not observed with the γ2 subunit proteins. A comparison of the AlphaFold2 predictions and the previously obtained cryo-EM structures presents new insights into the subunits’ modular structure and polymerization status. By performing experimental and computational studies, a deeper understanding of the complex structure of GABA_A receptors is provided. Hopefully, this study can pave the way to developing novel therapeutics for neuropsychiatric diseases. Full article

In the local building industry of Pakistan, pre-engineered steel building manufacturers mainly employ their own self-developed software and Excel sheets. These systems are based on empirical formulas mentioned in the AISI manual. Under this scenario, a need was found to validate AISI flow charts using commercial software like CUFSM 5.04 and ABAQUS R2019x. This study presents a validation of the CUFSM software and the American Iron and Steel Institute (AISI) Direct Strength Method (DSM) results of channel section flexural members using the non-linear finite element method employing ABAQUS. In this study, eight standard cold-formed channel-section (C-section) steel members were modeled and analyzed using ABAQUS to simulate realistic behavior under four-point loading conditions. The non-linear finite element models incorporated material and geometric non-linearities to capture the actual response of the steel elements. The results obtained from ABAQUS were compared with those predicted by the CUFSM and DSM, focusing on critical parameters such as nominal strength, buckling modes, and deformation patterns. During this study, it was observed that out of the selected sections, the AISI charts predict conservative and even unsafe flexural capacities in some of the cases concerning other methods, with a maximum difference of 14.03%. The differences obtained using DSM and ABAQUS when compared with the results of the AISI charts varies on both the plus and minus sides. This study will not only affect the industry in terms of innovative designs for efficient structures but also the community in regards to low-budget construction. Full article

Presently, the provision of safe drinking water is becoming a big challenge all over the world. In developing countries like Pakistan, many technical, financial and policy-related issues are hindering clean drinking water supply to communities. This study evaluates the performance of the drinking water supply system in Islamabad, starting from the Khanpur Dam to the consumer end via the Sangjani water treatment plant (SG-WTP). For this purpose, different physicochemical and biological parameters of water quality were analyzed and compared at four different locations in the Islamabad water supply network (also called the Khanpur Dam water supply network) for a period of one year. Statistical analyses such as the t-test, principal component analysis (PCA) and cluster analysis (CA) were performed to observe the variations in water quality parameters at the four locations. The results illustrate that the water quality upstream of the SG-WTP is declining due to various anthropogenic activities adding a variety of organic and inorganic pollutants into the water channel coming from the Khanpur Dam to the Sangjani plant. The water quality at the consumer end is deteriorating mainly due to algal growth and cracks in the water distribution network. As far as the performance of the SG-WTP is concerned, it is currently in good working condition and treating most of the water pollution coming from the Khanpur Dam water. Proper repair, maintenance and regular monitoring are necessary for sustainable operation of the Islamabad water supply system. Full article

Natural dams are formed most often in narrow, steep valleys in high mountains. The outburst floods triggered by natural dam failures result in the topography and landforms successively being altered. Boulder bars are common natural structures that are selected here to quantitatively evaluate the impact of outburst floods on the topographical and landform variations in downstream channels. In this study, we selected the Sedongpu natural dam on the Yarlung Tsangpo River formed as a result of a landslide in 2018 as an example, and studied the geomorphological changes in a river reach located 173 km downstream of the Sedongpu natural dam. The sizes and shapes of the boulder bars in this area were statistically analyzed. The results show that there are three shape types of boulder bars in this area, i.e., sickle, bamboo leaf and oval. Furthermore, it found that the relationship between the lengths and widths of boulder bars is similar before and after outburst floods, as is the relationship between perimeters and lengths of boulder bars, which means these relationships are not affected by outburst floods. And the perimeters of boulder bars are almost twice their lengths. In addition, the relationship between the areas and lengths of boulder bars follows a power function. The most important finding is that the riverine morphological features conserved self-similarity due the influence of the outburst flood erosion triggered by a natural dam failure. This finding adds to the previous observations since dam failures introduce sudden and dominating impacts on river systems. Full article

The Geostationary Interferometric InfraRed Sounder (GIIRS) provides a novel opportunity to acquire high-spatiotemporal-resolution atmospheric information. Previous studies have demonstrated the positive impacts of assimilating GIIRS radiances from either long-wave temperature or middle-wave water vapor bands on modeling high-impact weather processes. However, the impact of assimilating both bands on forecast skill has been less investigated, primarily due to the non-identical geolocations for both bands. In this study, a locally cloud-resolving global model is utilized to assess the impact of assimilating GIIRS observations from both long-wave and middle-wave bands. The findings indicate that the GIIRS observations exhibit distinct inter-channel error correlations. Proper inflation of these errors can compensate for inaccuracies arising from the treatment of the geolocation of the two bands, leading to a significant enhancement in the usage of GIIRS observations from both bands. The assimilation of GIIRS observations not only markedly reduces the normalized departure standard deviations for most channels of independent instruments, but also improves the atmospheric states, especially for temperature forecasting, with a maximum reduction of 42% in the root-mean-square error in the lower troposphere. These improvements contribute to better performance in predicting heavy rainfall. Full article

Scorpions, an ancient group of venomous invertebrates, have existed for over 430 million years. Their toxins, important for predation and defense, exhibit a variety of biological and pharmacological activities. Research on scorpion toxins has spanned decades. Notably, the toxin genes of Mesobuthus martensii (Scorpiones: Buthidae), a well-known Chinese herbal medicine, have been described at genomic and proteomic levels. However, previous studies primarily focused on the toxin genes expressed in the venom glands, overlooking their expression in multiple tissues. This study analyzed transcriptomes from 14 tissues of M. martensii. Gene annotation revealed 83 toxin and toxin-like genes, including those affecting sodium, potassium, calcium, and chloride ion channels. Approximately 70% of toxin genes were highly expressed in the vesicle; additionally, some exhibited low or no expression in the vesicle while showing high expression in other tissues. Beyond the vesicle, high expression levels of toxin genes were observed in metasoma segments II-V, blood, lateral eyes, chelicerae, legs, pedipalp chelae, femurs, and patellae. This expression pattern suggests that toxin genes are recruited from multiple tissues and may help prevent intraspecific harm during courtship and competition for prey. These findings inspire further research into the evolutionary recruitment process of scorpion toxins. Full article

This paper presents a concomitant observer-based multi-level fault-tolerant control (FTC) for near-space vehicles (NSVs) with a new type dissimilar redundant actuation system (NT-DRAS). When NSV flight control system faults occur in NT-DRAS and attitude-corresponding sensors, the NSV hybrid output states, including the concomitant observer usable states and the real system states, are applied to solve the FTC gain by using the linear quadratic regulator (LQR) technique. Furthermore, since NT-DRAS is used in NSVs, a multi-level (actuation system level and flight control level) FTC strategy integrating NT-DRAS channel switching and flight control LQR is proposed for complex and worsening fault cases. The most important finding is that though the proposed strategy is applicable for worsening fault cases in NSVs, systematic and accurate criteria for the process being performed are necessary and can improve the FTC efficiency with minimal FTC resources. Additionally, such criteria can improve the NSV’s responsiveness to comprehensive faults, provided that the real-time performance of the fault detection and diagnosis (FDD) scheme can be further optimized. The concomitant observer convergence and the multi-level FTC strategy have been verified by numerical simulations based on the Matlab/Simulink platform. Full article