Search Results (3,635)

Background: In broiler chickens, the efficient utilization of macro- and micronutrients is influenced by various metabolic pathways that are closely linked to feed efficiency (FE), a critical metric in poultry industry, with residual feed intake (RFI) as the preferred proxy. Feed restriction is considered an approach to address the underlying molecular mechanisms of feed conversion. We hypothesized that broiler chickens with divergent RFI subjected to quantitative feed restriction differ in their pattern of molecular pathways for efficient nutrient utilization in liver as post-absorptive tissue. Methods: Cobb 500FF broiler chickens divergent for RFI (n = 112) were feed-restricted from day 9 until market weight at day 33–37 post-hatch. Based on a previous trial, feed restriction levels were set at 92% (low-RFI birds) and 80% (high-RFI birds) relative to the control groups. Transcriptomic analyses of the liver were conducted. Results: Due to the interaction of the RFI group and feeding regimen, a total of 140 to 507 differentially expressed genes were identified for the respective contrasts, with implications for hepatic metabolism and cellular stress response. Although the broilers did not realize their full growth potential under restrictive feeding (12.4% reduced body weight vs. controls, p = 0.094), the gene expression patterns indicate a lower susceptibility to blood coagulation (KNG1, FGG, and FGB), suggesting that controlled and mild feed restriction could lead to health benefits in less feed-efficient broilers. Moreover, FE traits are shown to be linked to cellular detoxification processes (MGST3 and CYP2AC2) and triacylglycerol syntheses (MOGAT1 and LPIN1). Conclusions: Divergent transcriptional profiles between broiler groups under varied caloric conditions indicate potential for optimizing nutritional management strategies. Full article

The choice of constitutive model significantly affects the accuracy of concrete perforation simulation. This study analyzes four concrete constitutive models, HJC, RHT, KCC, and TCK, focusing on their strength models, damage evolution, and strain rate effects. Combining the damage pattern and erosion cracks, the effectiveness of the four constitutive models in simulating the penetration of reinforced concrete targets is evaluated using LS-DYNA 11.0. The results indicate that the RHT and TCK models accurately depict the concrete damage and failure modes under the same test conditions. In contrast, the KCC and HJC models demonstrate superior capability in predicting the residual velocity of the projectile. Additionally, this study highlights the significant impact of the erosion parameters on the simulation results. This study offers a valuable reference for the application and parameter set of constitutive models in simulating concrete target perforation. Full article

This article delves into the landscape of the plant-based food industry, exploring innovative market entry strategies and their interplay with the evolving food market and shifting consumer preferences and lifestyles. Adopting a multidimensional approach, the study reflects diverse perspectives at play. Through a comparative analysis of strategic promotion and growth strategies employed by both innovative startups and established industry giants in the plant-based alternatives sector in Germany, the study aims to capture the challenges faced by the ‘newer’ wave of plant-based alternatives. This examination is particularly relevant for navigating the complexities of entering a competitive market. Striving to penetrate evolving consumer lifestyles, the study draws insightful comparisons with their “older” counterparts. In addition, through in-depth interviews with selected companies, the study investigates the entry methods of startups venturing into the German market, aiming to identify parallels and contrasts in market strategies with established brands. This provides valuable insights into innovation within the alternative food market. Furthermore, the study explores the process of brand “platformization” by scrutinizing the websites of the selected companies and analyzing customers’ online reviews of their brand experience. This comprehensive analysis unveils distinctive patterns in communication strategies, brand positioning, and consumer engagement, offering insights into the ways plant-based innovation shapes new, caring lifestyles. The main findings include a strong correlation among dimensions related to food quality, use, and taste, indicating a holistic approach to food consumption facilitated by online platforms. Full article

Monitoring crude oil spills in coastal areas is challenging due to limitations in traditional in situ methods. Electrical resistivity imaging (ERI) offers a high-resolution approach to monitoring the subsurface spatial distribution of crude oil, but its effectiveness in highly-resistive, unsaturated coastal sands with varying salinity remains unexplored. This study assessed the effectiveness of ERI for monitoring crude oil spills in sandy soil using a 200 × 60 × 60 cm 3D sandbox filled with medium-fine-grained sand under unsaturated conditions. Two liters of crude oil were spilled under controlled conditions and monitored for 48 h using two surface ERI transects with 98 electrodes spaced every 2 cm and a dipole–dipole electrode array. The influence of varying salinity was simulated by varying the pore-fluid conductivities at four levels (0.6, 20, 50, and 85 mS/cm). After 48 h, the results show a percentage resistivity increase of 980%, 280%, 142%, and 70% for 0.6, 20, 50, and 85 mS/cm, respectively. The crude oil migration patterns varied with porewater salinity as higher salinity enhanced the crude oil retention at shallow depth. High salinity produces a smaller resistivity contrast, thus limiting the sensitivity of ERI in detecting the crude oil contaminant. These findings underscore the need to account for salinity variations when designing remediation strategies, as elevated salinity may restrict crude oil migration, resulting in localized contaminations. Full article

A systematic understanding of the spatial-temporal evolution patterns of cultivated land fragmentation (CLF), its driving factors, and its relationship with the expansion of urban and rural construction land is essential for identifying strategies to mitigate CLF in rapidly urbanizing regions. This study combined landscape fragmentation with ownership fragmentation, analyzing CLF through three dimensions: resource endowment, spatial concentration, and convenience of utilization, with eight selected indicators. By comparing village-level data from 2013 to 2022, we explored the key drivers of CLF and its conflicts with urban and rural construction land expansion. The findings indicate a clear spatial variation in village-level CLF in Ezhou, characterized by low fragmentation in the northwest and northeast, and high fragmentation in the southwest and central regions. This pattern is in contrast to Ezhou’s economic development, which decreased progressively from east to north and south. Over the study period, village-level CLF in Ezhou evolved from being primarily moderately and relatively severely fragmented to predominantly severely and relatively severely fragmented, with an overall declining trend and more pronounced polarization. At the same time, the CLF within the village region demonstrated notable spatial clustering features, with a rapid increase observed between 2013 and 2022. It was also discovered that CLF is driven by various factors, with the main influences being the proportion of construction land, land use intensity, and population density. Cultivated land is the main source of both urban construction land (UCL) and rural construction land (RCL), with average contribution rates of 46.47% and 62.62%, respectively. This research offers empirical evidence for rapid urbanization and serves as a critical reference for rural revitalization and coordinated urban–rural development, with potential guidance for future policy formulation and implementation. Full article

Background/Objectives: Alcohol consumption and poor dietary habits are on the rise in the United States, posing significant challenges to public health due to their contribution to chronic diseases such as liver failure. While associations between alcohol consumption patterns and diet quality have been explored, the relationship between specific alcoholic beverage types and diet quality remains underexamined. This study aims to compare diet quality among consumers of different alcoholic beverage types. Methods: We conducted a cross-sectional analysis of 1917 current alcohol drinkers from the National Health and Nutrition Examination Survey (NHANES) who completed a 24 h dietary recall survey. Diet quality was assessed using the Healthy Eating Index (HEI), with higher scores indicating superior diet quality. Multivariable logistic regression models were employed to assess differences in HEI between consumers of various alcoholic beverage types, using wine-only drinkers as the reference group and controlling for demographic, socioeconomic, lifestyle, and metabolic syndrome variables. Results: Beer-only drinkers were more likely to have lower income, higher rates of cigarette smoking, and insufficient physical activity compared to other alcohol consumers. In the fully adjusted multivariable model, beer-only drinkers had an HEI score that was 3.12 points lower than wine-only drinkers. In contrast, liquor/cocktail-only and multiple-type drinkers had similar HEI scores to wine-only drinkers. Conclusions: Beer-only consumption is associated with poorer diet quality among alcohol drinkers. Targeted patient education and public health campaigns may be effective in addressing the combined impact of alcohol consumption and poor diet quality on chronic disease risk. Full article

The fragmented forests of the Kenya highlands, known for their exceptional species richness and endemism, are among the world’s most important biodiversity hotspots. However, detailed studies on the fauna of these ecosystems—especially specialist species that depend on moist forests, which are particularly threatened by habitat fragmentation—are still limited. In this study, we used mitochondrial genes (cytochrome b and the displacement loop) and a nuclear marker (retinol-binding protein 3) to investigate genetic and morphological diversity, phylogenetic associations, historical divergence, population dynamics, and phylogeographic patterns in two rodent species—the soft-furred mouse (Praomys jacksoni) and the African wood mouse (Hylomyscus endorobae)—across Kenya’s forest landscapes. We found a complex genetic structure, with P. jacksoni exhibiting greater genetic diversity than H. endorobae. The Mt. Kenya P. jacksoni populations are significantly genetically different from those in southwestern forests (Mau Forest, Kakamega Forest, and Loita Hills). In contrast, H. endorobae presented no observable biogeographic structuring across its range. The genetic diversity and geographic structuring patterns highlighted selectively strong effects of forest fragmentation and differing species’ ecological and evolutionary responses to these landscape changes. Our findings further underscore the need for expanded sampling across Kenya’s highland forests to better understand species’ changing diversity and distribution patterns in response to the impacts of human-mediated habitat changes. These insights are critical for informing conservation strategies to preserve biodiversity better in this globally important region. Full article

Light non-aqueous phase liquids (LNAPLs), which include various petroleum products, are a significant source of groundwater contamination globally. Once introduced into the subsurface, these contaminants tend to accumulate in the vadose zone, causing chronic soil and water pollution. The vadose zone often contains lens-shaped bodies with diverse properties that can significantly influence the migration and distribution of LNAPLs. Understanding the interaction between LNAPLs and these lens-shaped bodies is crucial for developing effective environmental management and remediation strategies. Prior research has primarily focused on LNAPL behavior in homogeneous media, with less emphasis on the impact of heterogeneous conditions introduced by lens-shaped bodies. To investigate the impact of lens-shaped structures on the migration of LNAPLs and to assess the specific effects of different types of lens-shaped structures on the distribution characteristics of LNAPL migration, this study simulates the LNAPL leakage process using an indoor two-dimensional sandbox. Three distinct test groups were conducted: one with no lens-shaped aquifer, one with a low-permeability lens, and one with a high-permeability lens. This study employs a combination of oil front curve mapping and high-density resistivity imaging techniques to systematically evaluate how the presence of lens-shaped structures affects the migration behavior, distribution patterns, and corresponding resistivity anomalies of LNAPLs. The results indicate that the migration rate and distribution characteristics of LNAPLs are influenced by the presence of a lens in the gas band of the envelope. The maximum vertical migration distances of the LNAPL are as follows: high-permeability lens (45 cm), no lens-shaped aquifer (40 cm), and low-permeability lens (35 cm). Horizontally, the maximum migration distances of the LNAPL to the upper part of the lens body decreases in the order of low-permeability lens, high-permeability lens, and no lens-shaped aquifer. The low-permeability lens impedes the vertical migration of the LNAPL, significantly affecting its migration path. It creates a flow around effect, hindering the downward migration of the LNAPL. In contrast, the high-permeability lens has a weaker retention effect and creates preferential flow paths, promoting the downward migration of the LNAPL. Under conditions with no lens-shaped aquifer and a high-permeability lens, the region of positive resistivity change rate is symmetrical around the axis where the injection point is located. Future research should explore the impact of various LNAPL types, lens geometries, and water table fluctuations on migration patterns. Incorporating numerical simulations could provide deeper insights into the mechanisms controlling LNAPL migration in heterogeneous subsurface environments. Full article

We investigate the influence of land use and land cover (LU/LC) changes on soil erosion and chemical weathering processes within the Nonguén watershed in the Coastal Cordillera of south-central Chile. The watershed is divided into three sub-basins, each characterized by distinct LU/LC patterns: native forest and exotic plantations. A comprehensive geochemical analysis, including trace elements and lithium (Li) isotopes, was conducted on river water and suspended sediment samples collected from streams within these sub-basins to assess how land management practices, particularly plantation activities, influence the geochemical composition of river systems. Our results show that sub-basins dominated by exotic plantations exhibit significantly higher concentrations of major and trace elements in suspended sediments compared to sub-basins dominated by native forests. The elevated trace element concentrations are primarily attributed to increased physical erosion due to forestry activities such as clear-cutting and soil disturbance, which enhance sediment mobilization. Notably, concentrations of elements such as Fe, Al, and As in plantation-dominated sub-basins are raised to ten times higher than in native-dominated sub-basins. In contrast, sub-basins with native forest cover exhibit lower levels of sediment transport and trace element mobilization, suggesting that native vegetation exerts a stabilizing effect that mitigates soil erosion. Despite the substantial differences in sediment transport and element concentrations, Li isotopic data (δ⁷Li) show minimal fractionation across the different LU/LC types. This indicates that land use changes impact the chemical weathering processes less compared to physical erosion. The isotopic signatures suggest that physical erosion, rather than chemical weathering, is the dominant process influencing trace element distribution in plantation-dominated areas. The study provides critical insights into how forestry practices, specifically the expansion of exotic plantations, accelerate soil degradation and affect the geochemical composition of river systems. The increased sediment loads, and trace element concentrations observed in plantation-dominated sub-basins, raise concerns about the long-term sustainability of forest management practices, particularly regarding their impacts on water quality in urban catchment areas. These results are of significant relevance for environmental management and policy, as they underscore the need for more investigation and sustainable land use strategies to minimize soil erosion and preserve water resources in regions undergoing rapid LU/LC changes. Full article

Long non-coding RNAs (lncRNAs) are crucial in regulating secondary metabolite production in plants, but their role in artemisinin (ART) biosynthesis, a key anti-malarial compound from Artemisia annua, remains unclear. Here, by investigating high-artemisinin-producing (HAP) and lowartemisinin-producing (LAP) genotypes, we found that the final artemisinin content in A. annua is influenced by the quantity of the precursor compounds. We report on RNA deep sequencing in HAP and LAP genotypes. Based on the application of a stringent pipeline, 1419 novel lncRNAs were identified. Moreover, we identified 256 differentially expressed lncRNAs between HAP and LAP. We then established correlations between lncRNAs and artemisinin biosynthesis genes in order to identify a molecular framework for the differential expression of the pathway between the two genotypes. Three potential lncRNAs (MSTRG.33718.2, MSTRG.30396.1 and MSTRG.2697.4) linked to the key artemisinin biosynthetic genes (ADS: Amorpha-4,11-diene synthase, DXS: 1-deoxy-D-xylulose-5-phosphate synthase, and HMGS: 3-hydroxyl-3-methyglutaryl CoA synthase) were detected. Importantly, we observed that up-regulation of these lncRNAs positively modulates the target artemisinin biosynthetic genes, potentially leading to high artemisinin biosynthesis in HAP. In contrast, BAS (beta-amyrin synthase), which is involved in the artemisinin competing pathway, was strongly down-regulated in HAP compared to LAP, in line with the expression pattern of the linked lncRNA MSTRG.30396.1. By identifying and characterizing lncRNAs that are potentially linked to the regulation of key biosynthetic genes, this work provides new insights into the complex regulatory networks governing artemisinin production in A. annua. Such findings could pave the way for innovative approaches in metabolic engineering, potentially enhancing artemisinin yields and addressing challenges in sustainable production. Full article

The COVID-19 pandemic and similar public health emergencies have significantly impacted global travel patterns. Analyzing the recovery characteristics of subway station-level passenger flow during the pandemic recovery phase can offer unique insights into public transportation operations and guide practical planning efforts. This pioneering study constructs a station-level passenger flow recovery resilience (PFRR) index during the rapid recovery phase using subway AFC system swipe data. Additionally, it develops an analytical framework based on a multiscale geographically weighted regression (MGWR) model, the improved gray wolf optimization with Levy flight (LGWO), and light gradient boosting machine (LightGBM) regression to analyze passenger flow resilience on weekdays and weekends in relation to land use-related built environment types. Finally, SHAP attribution analysis is used to study the nonlinear relationships between built environment variables and PFRR index. The results show significant spatial heterogeneity in the impact of commercial, recreational, and residential land, as well as POI (points of interest) of leisure and shopping on PFRR. On weekdays, the most relevant built environment variables for PFRR are POI of enterprises and shopping numbers. In contrast, the contribution of built environment variables affecting PFRR of weekend is more balanced, reflecting the recovery of non-essential travel on weekends. Most land use-related built environment variables exhibit nonlinear associations with PFRR values. The proposed analytical framework shows significant performance advantages over other baseline models. This study provides unique insights into subway passenger flow characteristics and surrounding land use-related development layouts under the impact of public health emergencies. Full article

Background: Adolescence is a period of significant psychological, physical, and social changes. During this time, adolescents face increasing responsibilities, such as making educational and career decisions, managing peer relationships, and becoming more independent from their families. These changes are often accompanied by mood fluctuations and altered sleep patterns. This study aimed to explore the relationships between bright- and dark-side hypomania, insomnia, and various dimensions of health-related quality of life (HRQOL), such as self-esteem, family and peer relationships, social acceptance, and autonomy. Methods: A total of 1475 participants in mid-adolescence (mean age: 13.4 years; range: 11–16 years; 48.8% males) completed a series of self-reported questionnaires covering sociodemographic information, hypomania, including dark and bright-side hypomania, insomnia, and HRQOL. Results: Compared to participants with no or dark-side hypomania, participants with bright-side hypomania reported better HRQOL. Bright-side hypomania was significantly associated with favorable relationships with parents and home, peer relationships, and the school environment and with less insomnia. In contrast, dark-side hypomania showed significant associations with lower scores for self-esteem, moods and emotional states, peer relationships, social acceptance, the school environment, and more insomnia. Conclusions: Among a larger sample of adolescents, bright- and dark-side hypomania were associated with a broad, though specific variety of aspects of HRQOL and insomnia. Given that standardized programs are available to improve insomnia and resilience as a proxy of psychological well-being, such interventions may have the potential to improve adolescents’ psychological well-being and sleep quality concomitantly. Full article

The Eastern Tianshan region, influenced by the far-field effect of northward compression and expansion of the Qinghai-Xizang block, features highly developed Late Quaternary active faults that exhibit significant neotectonic activity. Historically, the Barkol-Yiwu Basin, located to the north of the Eastern Tianshan, experienced two major earthquakes in 1842 and 1914, each with a magnitude of M7¹/₂. In contrast, the Hami Basin on the southern margin of the Eastern Tianshan has no historical records of any major earthquakes, and its seismic potential, mechanisms, and future earthquake hazards remain unclear. Based on satellite image interpretation and field surveys, this study identified a relatively recent and well-preserved seismic surface rupture zone with good continuity in the Liushugou area of the western segment of the Northern Margin Fault of the Hami Basin (HMNF), which is the seismogenic structure responsible for the rupture. The surface rupture zone originates at Kekejin in the east, extends intermittently westward through Daipuseke Bulake and Liushugou, and terminates at Wuzun Bulake, with a total length of approximately 21 km. The rupture zone traverses the youngest geomorphic surface units, such as river beds or floodplains and first-order terraces (platforms), and is characterized by a series of single or multiple reverse fault scarps. The morphology of fault scarps is clear, presenting a light soil color with heights ranging from 0.15 m to 2.13 m and an average displacement of 0.56 m, suggesting that this surface rupture zone likely represents the most recent seismic event. Comparison with historical earthquake records in the Eastern Tianshan region suggests that the rupture zone may have been formed simultaneously with the Xiongkuer rupture zone by the 1842 M7¹/₂ earthquake along the boundary faults on both sides of the Barkol Mountains, exhibiting a flower-like structural pattern. Alternatively, it might represent a separate, unrecorded seismic event occurring shortly after the 1842 earthquake. The estimated magnitude of the associated earthquake is about 6.6~6.9. Given that surface-rupturing earthquakes have already occurred in the western segment, the study indicates that the Erdaogou–Nanshankou section of the HMNF has surpassed the average recurrence interval for major earthquakes, indicating a potential future earthquake hazard. Full article

We realize that a Sierpiński arrowhead curve (SAC) fills a Sierpiński gasket (SG) in the same manner as a Peano curve fills a square. Namely, in the limit of an infinite number of iterations, the fractal SAC remains self-avoiding, such that $SAC\subset SG$. Therefore, SAC differs from SG in the same sense as the self-avoiding Peano curve $PC\subset {\left[\mathrm{0,1}\right]}^2$ differs from the square. In particular, the SG has three-line segments constituting a regular triangle as its border, whereas the border of SAC has the structure of a totally disconnected fat Cantor set. Thus, in contrast to the SG, which has loops at all scales, the SAC is loopless. Consequently, although both patterns have the same similarity dimension $D=\ln 3/\ln 2$, their connectivity dimensions are different. Specifically, the connectivity dimension of the self-avoiding SAC is equal to its topological dimension $d_{\mathcal{l}}\left(SAC\right)=d=1$, whereas the connectivity dimension of the SG is equal to its similarity dimension, that is, $d_{\mathcal{l}}\left(SG\right)=D$. Therefore, the dynamic properties of SG and SAC are also different. Some other noteworthy features of the Sierpiński triangle are also highlighted. Full article

This study assesses the impact of climate change on the distribution of Ixodes ricinus, which transmits Lyme disease, a growing public health concern. Utilizing ensemble models from the R package ‘flexsdm’ and climate data from WorldClim, ENVIREM, and CliMond, we project habitat suitability changes for the focus species. The models, validated against Lyme disease incidence rates, predict a 1.5-fold increase in suitable habitats in Latvia, contrasted with a 4.5-fold decrease in suitable habitats within Ukraine over the coming decades. SHAP values are analyzed to determine the most influential climatic features affecting tick distribution, providing insights for future vector control and disease prevention strategies. The optimal bioclimatic environment for I. ricinus seems to be an intricate balance of moderate temperatures, high humidity, and sufficient rainfall (bio7, 14, 18, 29). Also, radiation during the wettest quarter (bio24) significantly influences tick distribution in northern countries. This implies an increased presence of ticks in Scandinavian countries, Baltic states, etc. These findings largely coincide with our projections regarding bioclimatic suitability for ticks in Latvia and Ukraine. These shifts reflect broader patterns of vector redistribution driven by global warming, highlighting the urgent need to adapt public health planning to the evolving landscape of vector-borne diseases under climate change. Full article