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17 pages, 8581 KiB  
Article
Oil Spill Mitigation with a Team of Heterogeneous Autonomous Vehicles
by André Dias, Ana Mucha, Tiago Santos, Alexandre Oliveira, Guilherme Amaral, Hugo Ferreira, Alfredo Martins, José Almeida and Eduardo Silva
J. Mar. Sci. Eng. 2024, 12(8), 1281; https://doi.org/10.3390/jmse12081281 - 30 Jul 2024
Viewed by 178
Abstract
This paper presents the implementation of an innovative solution based on heterogeneous autonomous vehicles to tackle maritime pollution (in particular, oil spills). This solution is based on native microbial consortia with bioremediation capacity, and the adaptation of air and surface autonomous vehicles for [...] Read more.
This paper presents the implementation of an innovative solution based on heterogeneous autonomous vehicles to tackle maritime pollution (in particular, oil spills). This solution is based on native microbial consortia with bioremediation capacity, and the adaptation of air and surface autonomous vehicles for in situ release of autochthonous microorganisms (bioaugmentation) and nutrients (biostimulation). By doing so, these systems can be applied as the first line of the response to pollution incidents from several origins that may occur inside ports, around industrial and extraction facilities, or in the open sea during transport activities in a fast, efficient, and low-cost way. The paper describes the work done in the development of a team of autonomous vehicles able to carry as payload, native organisms to naturally degrade oil spills (avoiding the introduction of additional chemical or biological additives), and the development of a multi-robot framework for efficient oil spill mitigation. Field tests have been performed in Portugal and Spain’s harbors, with a simulated oil spill, and the coordinate oil spill task between the autonomous surface vehicle (ASV) ROAZ and the unmanned aerial vehicle (UAV) STORK has been validated. Full article
(This article belongs to the Section Marine Pollution)
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25 pages, 4294 KiB  
Article
Novel Approach to Analyzing Friction Losses by Modeling the Microflow of Lubricating Oil between the Piston Rings and Cylinder in Internal Combustion Engines
by Piotr Wróblewski and Stanisław Kachel
Energies 2024, 17(15), 3697; https://doi.org/10.3390/en17153697 - 26 Jul 2024
Viewed by 286
Abstract
This work focuses on the evolution of lubrication wedge shaping in internal combustion piston engines, taking into account liquid microflows on curved surfaces and coating microgeometries. It introduces a new approach to the analysis of friction losses by simulating the microflow of lubricating [...] Read more.
This work focuses on the evolution of lubrication wedge shaping in internal combustion piston engines, taking into account liquid microflows on curved surfaces and coating microgeometries. It introduces a new approach to the analysis of friction losses by simulating the microflow of lubricating oil between the surfaces of piston rings cooperating with the cylinder surface. The models used take into account three types of microgeometry and material expansion. Key results indicate that microirregularities with a stereometry of 0.1–0.2 µm significantly influence the distribution of oil film thickness in the phase of maximum working pressure, which is critical for the functioning of the seal ring. The innovation of the work consists of demonstrating that, despite small changes in the friction force and power in the piston rings, changes in the minimum values of the oil film thickness are significant. The work highlights the failure to take into account microgeometry parameters in friction models, which leads to significant errors in the simulation results, especially in terms of oil film continuity and the contribution of mixed friction. The simulations also indicate that advanced geometric models with high mesh resolution are necessary only for the assessment of changes in oil film thickness during the highest pressure increase in the combustion chamber and taking into account various mixed friction conditions. The results suggest significant progress in engine design and performance, confirming the importance of advanced fluid and mixed friction models in piston engine lubrication research. Full article
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24 pages, 8791 KiB  
Article
Event-Triggered Neural Adaptive Distributed Cooperative Control for the Multi-Tug Towing of Unactuated Offshore Platform with Uncertainties and Unknown Disturbances
by Shaolong Geng, Yulong Tuo, Yuanhui Wang, Zhouhua Peng and Shasha Wang
J. Mar. Sci. Eng. 2024, 12(8), 1242; https://doi.org/10.3390/jmse12081242 - 23 Jul 2024
Viewed by 244
Abstract
An event-triggered neural adaptive cooperative control is proposed for the towing system (TS) with model parameter uncertainties and unknown disturbances. Different from ordinary multi-vessel formation control, the tugs and unactuated offshore platform in the TS are connected together by towlines, and the resultant [...] Read more.
An event-triggered neural adaptive cooperative control is proposed for the towing system (TS) with model parameter uncertainties and unknown disturbances. Different from ordinary multi-vessel formation control, the tugs and unactuated offshore platform in the TS are connected together by towlines, and the resultant tension of the towlines serves as the actual drag force for the platform. Initially, based on the radial basis function neural network (RBFNN), an adaptive RBFNN is designed to compensate unknown disturbances and model parameter uncertainties of the TS, and we use minimal learning parameter (MLP) algorithm to reduce the online learning parameters of adaptive RBFNN. Combined with dynamic surface technology and event-triggered control (ETC) mechanism, an event-triggered neural adaptive virtual controller is designed to obtain the desired drag force of the platform. According to the quadratic programming algorithm, the desired drag force is allocated as the desired tensions of towlines. Subsequently, the desired towline length and the desired position information of the tugs are obtained sequentially through the towline model and the position relationship between the tugs and the platform. Then, according to the desired positions of tugs, an event-triggered neural adaptive distributed cooperative controller is designed for achieving the multi-tug towing of the offshore platform. The ETC mechanism is introduced to reduce the communication burden within the TS and the execution frequency of the tugs’ thrusters. Finally, the stability of the closed-loop system is proven using the Lyapunov theory, and the ETC mechanism proves that no Zeno behavior occurs. The effectiveness of the ETC mechanism and the MLP-based adaptive RBFNN on the controllers of TS is verified through simulations and comparison analysis. Full article
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13 pages, 6112 KiB  
Article
Automatic Defect Detection Instrument for Spherical Surfaces of Optical Elements
by Yali Shi, Mei Zhang and Mingwei Li
Photonics 2024, 11(7), 681; https://doi.org/10.3390/photonics11070681 - 22 Jul 2024
Viewed by 303
Abstract
In order to realize automatic surface defect detection for large aperture precision spherical optical elements, an automatic surface defect detection instrument has been designed. The instrument consists of a microscopic imaging system, illumination system, motion scanning system, and a software algorithm system. Firstly, [...] Read more.
In order to realize automatic surface defect detection for large aperture precision spherical optical elements, an automatic surface defect detection instrument has been designed. The instrument consists of a microscopic imaging system, illumination system, motion scanning system, and a software algorithm system. Firstly, a multi-angle channel illumination source and a coaxial illumination source were designed. Bright and dark field images of surface defects were captured by cooperating with an automatic zoom microscope. Then, algorithms for scanning trajectory planning, image stitching, and intelligent defect recognition were designed to achieve full-aperture surface image acquisition and defect quantification detection. The automated defect detection process of the instrument is summarized and introduced. Finally, the experimental platform was constructed, which can work well for the optical elements with a maximum diameter of 400 mm and a relative aperture R/D value of 1. It takes about 15 min to detect an optical element with a diameter of 200 mm in dark-field imaging mode. As a result, the minimum line width of scratch detectable is 2 μm and the minimum diameter of pitting detectable is 4 μm. Clearly, the instrument can realize the automatic detection of surface defects of spherical optical elements, and has the advantages of a high efficiency, stability, reliability, quantification, and data traceability. Full article
(This article belongs to the Special Issue Optical Imaging and Measurements)
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17 pages, 10412 KiB  
Article
Bond Behavior and Failure Mechanisms of the Interface between Engineered Cementitious Composites and Shaped Steel
by Jiaojiao Pan, Zhenbin Huang, Tingting Lu and Mingke Deng
Buildings 2024, 14(7), 2233; https://doi.org/10.3390/buildings14072233 - 19 Jul 2024
Viewed by 338
Abstract
Due to their excellent ductility and crack-control ability, engineered cementitious composites (ECCs) combined with shaped steel can produce steel-reinforced engineering cementitious composite (SRECC) structures which exhibit significant advantages in prefabricated buildings. The interface bond behavior is the base for the cooperative working performance [...] Read more.
Due to their excellent ductility and crack-control ability, engineered cementitious composites (ECCs) combined with shaped steel can produce steel-reinforced engineering cementitious composite (SRECC) structures which exhibit significant advantages in prefabricated buildings. The interface bond behavior is the base for the cooperative working performance of the shaped steel and ECC. This study included push-out tests of one ordinary concrete control specimen and ten ECC specimens. The various parameters were the ECC compressive strength, fiber volume content, cover thickness, and the embedded length of shaped steel. The bond stress–slip curves at the loading and free end were obtained, and the effects of various parameters on the characteristic points of curves were analyzed. The results indicated that the ordinary concrete specimen failed in brittle splitting, with the cracks completely penetrating the surface of the specimen. Due to the fiber-bridging effect in ECCs effectively preventing the development and extension of cracks, the shaped steel at the free end was obviously pushed out, and the surrounding matrix maintained good integrity after testing finished. For ECC specimens, bond or splitting-bond failure occurred, exhibiting outstanding ductility. Compared with the ordinary concrete specimen, the standard ultimate and residual bond strength of ECC specimens improved by 37.9% and 27.4%, respectively. Besides the increase in ECC compressive strength, the fiber volume content and cover thickness had a significant positive influence on the ultimate and residual bond strength, whereas the effect of the embedded length was the opposite. Finally, the calculation equations of characteristic bond strength were proposed, and the calculated values matched well with the experimental values. Full article
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21 pages, 2356 KiB  
Review
Advancing Non-Line-of-Sight Communication: A Comprehensive Review of State-of-the-Art Technologies and the Role of Energy Harvesting
by Yasir Al-Ghafri, Hafiz M. Asif, Naser Tarhuni and Zia Nadir
Sensors 2024, 24(14), 4671; https://doi.org/10.3390/s24144671 - 18 Jul 2024
Viewed by 440
Abstract
Enhancing spectral efficiency in non-line-of-sight (NLoS) environments is essential as 5G networks evolve, surpassing 4G systems with high information rates and minimal interference. Instead of relying on traditional Orthogonal Multiple Access (OMA) systems to tackle issues caused by NLoS, advanced wireless networks adopt [...] Read more.
Enhancing spectral efficiency in non-line-of-sight (NLoS) environments is essential as 5G networks evolve, surpassing 4G systems with high information rates and minimal interference. Instead of relying on traditional Orthogonal Multiple Access (OMA) systems to tackle issues caused by NLoS, advanced wireless networks adopt innovative models like Non-Orthogonal Multiple Access (NOMA), cooperative relaying, Multiple Input Multiple Output (MIMO), and intelligent reflective surfaces (IRSs). Therefore, this study comprehensively analyzes these techniques for their potential to improve communication reliability and spectral efficiency in NLoS scenarios. Specifically, it encompasses an analysis of cooperative relaying strategies for their potential to improve reliability and spectral efficiency in NLoS environments through user cooperation. It also examines various MIMO configurations to address NLoS challenges via spatial diversity. Additionally, it investigates IRS settings, which can alter signal paths to enhance coverage and reduce interference and analyze the role of Unmanned Aerial Vehicles (UAVs) in establishing flexible communication infrastructure in difficult environments. This paper also surveys effective energy harvesting (EH) strategies that can be integrated with NOMA for efficient and reliable energy-communication networks. Our findings show that incorporating these technologies with NOMA not only enhances connectivity and spectral efficiency but also promotes a stable and environmentally sustainable data communication system. Full article
(This article belongs to the Section Sensor Networks)
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24 pages, 11966 KiB  
Article
Evaluation of Denoising and Voxelization Algorithms on 3D Point Clouds
by Sara Gonizzi Barsanti, Marco Raoul Marini, Saverio Giulio Malatesta and Adriana Rossi
Remote Sens. 2024, 16(14), 2632; https://doi.org/10.3390/rs16142632 - 18 Jul 2024
Viewed by 276
Abstract
Proper documentation is fundamental to providing structural health monitoring, damage identification and failure assessment for Cultural Heritage (CH). Three-dimensional models from photogrammetric and laser scanning surveys usually provide 3D point clouds that can be converted into meshes. The point clouds usually contain noise [...] Read more.
Proper documentation is fundamental to providing structural health monitoring, damage identification and failure assessment for Cultural Heritage (CH). Three-dimensional models from photogrammetric and laser scanning surveys usually provide 3D point clouds that can be converted into meshes. The point clouds usually contain noise data due to different causes: non-cooperative material or surfaces, bad lighting, complex geometry and low accuracy of the instruments utilized. Point cloud denoising has become one of the hot topics of 3D geometric data processing, removing these noise data to recover the ground-truth point cloud and adding smoothing to the ideal surface. These cleaned point clouds can be converted in volumes with different algorithms, suitable for different uses, mainly for structural analysis. This paper aimed to analyse the geometric accuracy of algorithms available for the conversion of 3D point clouds into volumetric models that can be used for structural analyses through the FEA process. The process is evaluated, highlighting problems and difficulties that lie in poor reconstruction results of volumes from denoised point clouds due to the geometric complexity of the objects. Full article
(This article belongs to the Special Issue New Perspectives on 3D Point Cloud II)
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21 pages, 22426 KiB  
Article
Intelligent Surveillance of Airport Apron: Detection and Location of Abnormal Behavior in Typical Non-Cooperative Human Objects
by Jun Li and Xiangqing Dong
Appl. Sci. 2024, 14(14), 6182; https://doi.org/10.3390/app14146182 - 16 Jul 2024
Viewed by 384
Abstract
Most airport surface surveillance systems focus on monitoring and commanding cooperative objects (vehicles) while neglecting the location and detection of non-cooperative objects (humans). Abnormal behavior by non-cooperative objects poses a potential threat to airport security. This study collects surveillance video data from civil [...] Read more.
Most airport surface surveillance systems focus on monitoring and commanding cooperative objects (vehicles) while neglecting the location and detection of non-cooperative objects (humans). Abnormal behavior by non-cooperative objects poses a potential threat to airport security. This study collects surveillance video data from civil aviation airports in several regions of China, and a non-cooperative abnormal behavior localization and detection framework (NC-ABLD) is established. As the focus of this paper, the proposed framework seamlessly integrates a multi-scale non-cooperative object localization module, a human keypoint detection module, and a behavioral classification module. The framework uses a serial structure, with multiple modules working in concert to achieve precise position, human keypoints, and behavioral classification of non-cooperative objects in the airport field. In addition, since there is no publicly available rich dataset of airport aprons, we propose a dataset called IIAR-30, which consists of 1736 images of airport surfaces and 506 video clips in six frequently occurring behavioral categories. The results of experiments conducted on the IIAR-30 dataset show that the framework performs well compared to mainstream behavior recognition methods and achieves fine-grained localization and refined class detection of typical non-cooperative human abnormal behavior on airport apron surfaces. Full article
(This article belongs to the Topic Artificial Intelligence Models, Tools and Applications)
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20 pages, 2486 KiB  
Review
Natural Killer-Based Therapy: A Prospective Thought for Cancer Treatment Related to Diversified Drug Delivery Pathways
by Jing Zang, Yijun Mei, Shiguo Zhu, Shaoping Yin, Nianping Feng, Tianyuan Ci and Yaqi Lyu
Pharmaceutics 2024, 16(7), 939; https://doi.org/10.3390/pharmaceutics16070939 - 14 Jul 2024
Viewed by 511
Abstract
Immunotherapy has been a research hotspot due to its low side effects, long-lasting efficacy, and wide anti-tumor spectrum. Recently, NK cell-based immunotherapy has gained broad attention for its unique immunological character of tumor identification and eradication and low risk of graft-versus-host disease and [...] Read more.
Immunotherapy has been a research hotspot due to its low side effects, long-lasting efficacy, and wide anti-tumor spectrum. Recently, NK cell-based immunotherapy has gained broad attention for its unique immunological character of tumor identification and eradication and low risk of graft-versus-host disease and cytokine storm. With the cooperation of a drug delivery system (DDS), NK cells activate tumoricidal activity by adjusting the balance of the activating and inhibitory signals on their surface after drug-loaded DDS administration. Moreover, NK cells or NK-derived exosomes can also be applied as drug carriers for distinct modification to promote NK activation and exert anti-tumor effects. In this review, we first introduce the source and classification of NK cells and describe the common activating and inhibitory receptors on their surface. Then, we summarize the strategies for activating NK cells in vivo through various DDSs. Finally, the application prospects of NK cells in tumor immunotherapy are also discussed. Full article
(This article belongs to the Special Issue Advanced Nanopharmaceutics for Anticancer Therapy)
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22 pages, 6161 KiB  
Article
Virtual Streamline Traction: Formation Cooperative Obstacle Avoidance Based on Dynamical Systems
by Yiping Liu, Jianqiang Zhang, Yuanyuan Zhang and Jiarui Wang
Appl. Sci. 2024, 14(14), 6087; https://doi.org/10.3390/app14146087 - 12 Jul 2024
Viewed by 333
Abstract
Formation obstacle avoidance is a critical aspect of cooperation among unmanned surface vehicles (USVs). In practical scenarios involving multiple USVs, managing obstacle avoidance during formation assembly and navigation is essential to ensure the success of cooperative tasks. This study devised a formation cooperative [...] Read more.
Formation obstacle avoidance is a critical aspect of cooperation among unmanned surface vehicles (USVs). In practical scenarios involving multiple USVs, managing obstacle avoidance during formation assembly and navigation is essential to ensure the success of cooperative tasks. This study devised a formation cooperative obstacle-avoidance scheme utilizing dynamical systems (DS). The traditional interfered fluid dynamical system (IFDS) applied in two-dimensional planes was enhanced to address local minima issues. Furthermore, robust virtual structure patterns were implemented to effectively decouple velocity vectors. Streamlines were optimized by adjusting velocity amplitudes within specific distance intervals, facilitating precise formation assembly amidst multiple obstacles. Additionally, a novel inter-vehicle disturbance method, distinct from the IFDS, was developed to enhance inter-vehicle collision avoidance. The effectiveness of the proposed method in enabling USV formations to adeptly navigate obstacles while maintaining formation integrity and collision-avoidance capabilities was analyzed theoretically and confirmed through simulation. Full article
(This article belongs to the Special Issue Modeling, Guidance and Control of Marine Robotics)
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16 pages, 2492 KiB  
Article
Miniaturizing Nanotoxicity Assays in Daphnids
by Dimitrios Kakavas, Konstantinos Panagiotidis, Keith D. Rochfort and Konstantinos Grintzalis
Animals 2024, 14(14), 2046; https://doi.org/10.3390/ani14142046 - 12 Jul 2024
Viewed by 355
Abstract
The rapid progress of the modern world has resulted in new materials and products created at an accelerating pace. As such, nanoparticles have widespread applications and often find their way into the aquatic ecosystem. In the case of freshwater ecosystems, one of the [...] Read more.
The rapid progress of the modern world has resulted in new materials and products created at an accelerating pace. As such, nanoparticles have widespread applications and often find their way into the aquatic ecosystem. In the case of freshwater ecosystems, one of the commonly used bioindicators species used for pollution assessment is Daphnid magna. The Organization for Economic Co-operation and Development (OECD), and other organizations such as the European Chemicals Agency (ECHA) and Environmental Protection Agency (EPA), have set guidelines for acute toxicity testing in daphnids that are severely lacking in terms of information on the characteristics of the exposure vessel when studying the adverse effects of nanoparticles (NPs). Understanding the toxicity mechanisms of nanomaterials is imperative given the scarcity of information on their adverse effects. Furthermore, miniaturization of nanotoxicity assays can reduce the number of daphnids used, as well as the cost and nanomaterial waste, and provide results even at the individual animal level with enhanced reproducibility of testing. In this study, the impact of the exposure vessel on the observed physiological changes of daphnids was investigated for a silver nano ink. Exposures in eleven commercially available vessels; nine made of plastic and two made of glass were compared for 24 h. The effect of surface to volume ratio of the exposure vessel and the animal number or “crowding” during exposure was investigated in the context of miniaturizing biomarker assays as alternatives to traditional experimental setups in Daphnid magna. Toxicity curves showed differences depending on the vessel used, while a novel feeding rate assay and the activity of key enzymes were assessed as physiology endpoints. Full article
(This article belongs to the Special Issue Ecotoxicology in Aquatic Animals)
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17 pages, 3500 KiB  
Article
Study on Safety Mining Technology of Gob in Stopping Face by Replacing Pressure Equalization with Gob Pumping—A Case Study of Sitai Mine
by Yun Xu, Guofu Li, Chenglin Huang, Jie Wang, Liangliang Liu and Hao Shao
Appl. Sci. 2024, 14(13), 5965; https://doi.org/10.3390/app14135965 - 8 Jul 2024
Viewed by 628
Abstract
Gas control in the upper corner of the natural coal mining face with high gas is always a difficult problem that troubles the safe production of the working face. Among them, a high gas-prone natural coal mining face with ground air leakage is [...] Read more.
Gas control in the upper corner of the natural coal mining face with high gas is always a difficult problem that troubles the safe production of the working face. Among them, a high gas-prone natural coal mining face with ground air leakage is more likely to cause gas and CO to exceed limits in the corner of the working surface and is difficult to control. The traditional treatment methods often have some problems; for example, it is easy to increase air leakage in the gob with the method of gas extraction in the gob, which is not conducive to the prevention and control of spontaneous combustion of coal in the gob. At present, the more effective method is the pressure-equalization method. However, the pressure-equalization measures need to establish a complex pressure-equalization system, and close cooperation between the systems is required; once the system power fails or equipment failure occurs, the pressure-equalization state changes randomly, and it is easy to cause gas over-limits and other faults. Therefore, this paper presents a new method to control gas in the gob of a coal seam by pumping the gob of the upper-adjacent layer, using the negative pressure of pumping, and balancing the negative pressure of the upper-adjacent layer and the gob of the coal seam to form a new pressure-equalization relationship. This method can prevent the toxic and harmful gases in the goaf of the upper-adjacent layer from escaping into the passageway of the gob of the local coal seam, reduce the air leakage in the goaf, and benefit the gas control and spontaneous coal combustion prevention in the goaf. Full article
(This article belongs to the Section Earth Sciences)
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13 pages, 4607 KiB  
Article
Adjacent Reaction Sites of Atomic Mn2O3 and Oxygen Vacancies Facilitate CO2 Activation for Enhanced CH4 Production on TiO2-Supported Nickel-Hydroxide Nanoparticles
by Praveen Kumar Saravanan, Dinesh Bhalothia, Amisha Beniwal, Cheng-Hung Tsai, Pin-Yu Liu, Tsan-Yao Chen, Hong-Ming Ku and Po-Chun Chen
Catalysts 2024, 14(7), 410; https://doi.org/10.3390/catal14070410 - 28 Jun 2024
Viewed by 493
Abstract
The catalytic conversion of carbon dioxide (CO2) to methane (CH4) through the “Sabatier reaction”, also known as CO2 methanation, presents a promising avenue for establishing a closed carbon loop. However, the competitive reverse water gas shift (RWGS) reaction [...] Read more.
The catalytic conversion of carbon dioxide (CO2) to methane (CH4) through the “Sabatier reaction”, also known as CO2 methanation, presents a promising avenue for establishing a closed carbon loop. However, the competitive reverse water gas shift (RWGS) reaction severely limits CH4 production at lower temperatures; therefore, developing highly efficient and selective catalysts for CO2 methanation is imperative. In this regard, we have developed a novel nanocatalyst comprising atomic scale Mn2O3 species decorated in the defect sites of TiO2-supported Ni-hydroxide nanoparticles with abundant oxygen vacancies (hereafter denoted as NiMn-1). The as-prepared NiMn-1 catalyst initiates the CO2 methanation at a temperature of 523 K and delivers an optimal CH4 production yield of 21,312 mmol g−1 h−1 with a CH4 selectivity as high as ~92% at 573 K, which is 45% higher as compared to its monometallic counterpart Ni-TiO2 (14,741 mmol g−1 h−1). Physical investigations combined with gas chromatography analysis corroborate that the exceptional activity and selectivity of the NiMn-1 catalyst stem from the synergistic cooperation between adjacent active sites on its surface. Specifically, the high density of oxygen vacancies in Ni-hydroxide and adjacent Mn2O3 domains facilitate CO2 activation, while the metallic Ni domains trigger H2 splitting. We envision that the obtained results pave the way for the design of highly active and selective catalysts for CO2 methanation. Full article
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21 pages, 2820 KiB  
Article
The Impact of Virgin and Aged Microstructured Plastics on Proteins: The Case of Hemoglobin Adsorption and Oxygenation
by Florent Saudrais, Marion Schvartz, Jean-Philippe Renault, Jorge Vieira, Stéphanie Devineau, Jocelyne Leroy, Olivier Taché, Yves Boulard and Serge Pin
Int. J. Mol. Sci. 2024, 25(13), 7047; https://doi.org/10.3390/ijms25137047 - 27 Jun 2024
Viewed by 502
Abstract
Plastic particles, particularly micro- and nanoparticles, are emerging pollutants due to the ever-growing amount of plastics produced across a wide variety of sectors. When plastic particles enter a biological medium, they become surrounded by a corona, giving them their biological identity and determining [...] Read more.
Plastic particles, particularly micro- and nanoparticles, are emerging pollutants due to the ever-growing amount of plastics produced across a wide variety of sectors. When plastic particles enter a biological medium, they become surrounded by a corona, giving them their biological identity and determining their interactions in the living environment and their biological effects. Here, we studied the interactions of microstructured plastics with hemoglobin (Hb). Virgin polyethylene microparticles (PEMPs) and polypropylene microparticles (PPMPs) as well as heat- or irradiation-aged microparticles (ag-PEMPs and ag-PPMPs) were used to quantify Hb adsorption. Polypropylene filters (PP-filters) were used to measure the oxygenation of adsorbed Hb. Microstructured plastics were characterized using optical microscopy, SAXS, ATR-FTIR, XPS, and Raman spectroscopy. Adsorption isotherms showed that the Hb corona thickness is larger on PPMPs than on PEMPs and Hb has a higher affinity for PPMPs than for PEMPs. Hb had a lower affinity for ag-PEMPs and ag-PPMPs, but they can be adsorbed in larger amounts. The presence of partial charges on the plastic surface and the oxidation rate of microplastics may explain these differences. Tonometry experiments using an original method, the diffuse reflection of light, showed that adsorbed Hb on PP-filters retains its cooperativity, but its affinity for O2 decreases significantly. Full article
(This article belongs to the Special Issue Hemoglobins: Structural, Functional and Evolutionary Characterization)
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27 pages, 2446 KiB  
Article
Intermolecular Electrostatic Interactions in Cytochrome c Protein Monolayer on Montmorillonite Alumosilicate Surface: A Positive Cooperative Effect
by Svetlana H. Hristova and Alexandar M. Zhivkov
Int. J. Mol. Sci. 2024, 25(13), 6834; https://doi.org/10.3390/ijms25136834 - 21 Jun 2024
Viewed by 484
Abstract
Montmorillonite (MM) crystal nanoplates acquire anticancer properties when coated with the mitochondrial protein cytochrome c (cytC) due to the cancer cells’ capability to phagocytize cytC-MM colloid particles. The introduced exogenous cytC initiates apoptosis: an irreversible cascade of biochemical reactions leading to cell death. [...] Read more.
Montmorillonite (MM) crystal nanoplates acquire anticancer properties when coated with the mitochondrial protein cytochrome c (cytC) due to the cancer cells’ capability to phagocytize cytC-MM colloid particles. The introduced exogenous cytC initiates apoptosis: an irreversible cascade of biochemical reactions leading to cell death. In the present research, we investigate the organization of the cytC layer on the MM surface by employing physicochemical and computer methods—microelectrophoresis, static, and electric light scattering—to study cytC adsorption on the MM surface, and protein electrostatics and docking to calculate the local electric potential and Gibbs free energy of interacting protein globules. The found protein concentration dependence of the adsorbed cytC quantity is nonlinear, manifesting a positive cooperative effect that emerges when the adsorbed cytC globules occupy more than one-third of the MM surface. Computer analysis reveals that the cooperative effect is caused by the formation of protein associates in which the cytC globules are oriented with oppositely charged surfaces. The formation of dimers and trimers is accompanied by a strong reduction in the electrostatic component of the Gibbs free energy of protein association, while the van der Waals component plays a secondary role. Full article
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