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Cell culture in two dimensions has been the main instrument in cellular and molecular biology. But there are limitations to two-dimensional culture when it comes to tissue engineering and in vivo reproduction. Tissue engineering technology enabled the creation of biomedical scaffolds, which are mostly utilized to biofabricate different artificial human organs. Tissue architecture that encourage cell proliferation can be produced using direct bioprinting technology. The development of bioinks for 3D bioprinting is consistently seen as a problem in the domains of biofabrication and tissue engineering. This study aimed to determine if Fibroblasts and Keratinocytes could grow on hydrogel scaffolds as efficiently as they can in the culture plates. Melanocytes were co-cultured, and the production of melanin was assessed in a two- and three-dimensional culture system. Scaffolds were fabricated using 8% alginate and 6% gelatin and 3D-printed using a cell link printer. FTIR was used to determine the precise composition of the gels. SEM analysis was performed for the cells present in gel and the topology of the cells. In addition, 8% alginate and 6% alginate gel scaffolds were analyzed for swelling and degradation over time in the cell growth medium and PBS. Furthermore, a gene expression study of cell cultures on scaffolds was performed through qPCR. A live/dead assay was performed to determine cell viability for cells grown on scaffolds for 7, 14, and 21 days. Most of the cells were shown to be viable, similar to the control cells grown on a plate. The findings from the SEM showed that cells were grown on the gel surface, remained viable even after 21 days, and displayed circular cells stacked three-dimensionally on the gel surface in the 3D scaffold. The MTT assay was performed to check the viability of cells cultured on a 3D-printed scaffold for 1, 5, and 15 days. We observed about 40% viable cells after 15 days, as shown by the MTT assay. Furthermore, a co-culture study with Melanocyte showed an increased production of melanin in a 3D culture as compared to a 2D culture. Our findings suggest that an alginate and gelatin polymer can be used as a cellular matrix for epithelial cell culture. Further, in vivo and ex vivo experiments are needed to validate the results for future applications in tissue engineering for wound healing and other tissue engineering domains. Full article

In this study, biofilm formation and the production of key virulence factors were systematically evaluated across 33 strains of Pseudomonas aeruginosa isolated from diverse hospital environments in Tunisia. Among these, 13 strains demonstrated strong biofilm-forming capacities. Adding glucose (9%, w/v) to the culture medium generally enhanced biofilm development, indicating that glucose supplementation may promote biofilm formation in clinical isolates. The 13 selected biofilm-forming strains exhibited a consistent production of critical virulence factors, including pyocyanin, rhamnolipid, and LasA protease, known for its staphylolytic activity. However, profiles of extracellular polysaccharide (EPS) production and motility showed considerable heterogeneity among the strains, suggesting a strain-specific regulation of these traits. Based on a comprehensive analysis of biofilm formation and virulence expression, strain 2629 was chosen as a model organism due to its robust biofilm-producing ability and high virulence factor output. This strain was used in subsequent in vitro assays to evaluate the anti-biofilm potential of a commercial disinfectant containing peracetic acid and other active agents. Results indicated that a 3% (v/v) concentration of the disinfectant, applied for 5 min, was nearly sufficient to eradicate the biofilm formed by the model strain. These findings underscore the importance of selecting a representative biofilm-forming strain for accurate in vitro assessments of disinfectant activity. Full article

Background: This study encapsulates an in-depth correlation analysis for the biological activities (cytotoxicity, antimicrobial, and α-amylase inhibition) vs. the phytochemical classes (flavonoids “FV” and alkaloid “AL”) present in fenugreek seed extract. Methodology: Cell cultures for different cell lines were used to assess the cytotoxicity and selectivity (IC₅₀ value), agar diffusion assay was used to determine the MIC and MBC for different bacteria and fungi, whereas α-amylase inhibition was studied to evaluate the antidiabetic potential for the forty-five different origins of fenugreek seed extracts. An in-house analysis for the phytochemical classes of flavonoids (rutin, RT; quercetin, QT; luteolin, LT; kaempferol, KF) and alkaloid (trigonelline, TG) was performed for the seed extracts. Results: A lower IC₅₀ value (14.7 ± 1.46 µg/mL) was recorded for the IR3M extract against the HT29, MCF7 (13.03 ± 1.95 µg/mL), and MRC5 (14.58 ± 2.92 µg/mL) cell line. The extract with the lower IC₅₀ value (8.17 ± 0.73 µg/mL) against HepG2 was IR2M. For the antimicrobial activity, a lower MIC value (6.3 mg/mL) was observed for E2C, E2M, E3C, and I3H extracts against SF and for the E1M, Y3C, IR2H, IR3H, and IR3C extracts against SA. The lowest MBC value (12.5 mg/mL) was seen for E2C, E2M, E3C, and I3H against SF as well as for the extracts E1M, Y3C, IR2H, IR3H, and IR3C against SA. The extracts of Q1H (49.07 ± 2.45 µg/mL) and Y3C (43.65 ± 2.97 µg/mL) exhibited IC₅₀ values comparable to the standard drugs tested for α-amylase inhibition. The statistical models were of Pearson’s correlation. Principal component analysis (PCA) and a paired t-test established a strong positive correlation for the FV (QT, KF, LT) and alkaloid (TG) (p < 0.05) in the biological activities (cytotoxicity, antimicrobial, and α-amylase inhibition), thereby suggesting a substantial role for these phytochemical classes in the traditional and medicinal uses of fenugreek seeds. Conclusions: The FV and alkaloid are the key to impart the biological properties to the fenugreek seeds, hence their presence is utmost in the fenugreek seeds. This research work may be used as marker to help authenticate the fenugreek seeds for the quality variation in the major phytochemical classes. Full article

The Santa Barbara Sense of Direction scale (SBSoD) is a self-report scale that assesses the ability to orient oneself in the environment. With the current study, we validated the Danish version of the SBSoD (SBSoDDK) and compared performance on the SBSoD across samples with different nationalities. We collected data for a Danish sample consisting of 119 Danish university students and received data from studies validating other versions of the SBSoD using American, German, Japanese, Chinese, Turkish, and British samples. The internal consistency and convergent and discriminant validity of the SBSoDDK, which exhibited a two-factor structure, were affirmed, and differences in performance on the SBSoD across samples with different nationalities were identified and may be linked to cultural variations in the sense of direction. The current study has certain weaknesses, including using nationality as a proxy for culture and variations in the gender compositions of the samples. Future studies should focus on addressing the current study’s weaknesses and revising and further validating the SBSoD. Full article

The effective implementation of preparedness and response strategies toward climate change resilience has evolved into a technical, sociopolitical, and communication issue. We argue that, for climate communication to effectively contribute to community resilience, it demands meaningful dialogue and engagement to facilitate understanding. Using the risk communication theory, we assessed the impact of complex English language on climate change understanding in Nigerian communities where local languages are predominant. Through surveys and semi-structured interviews, we found that current communication strategies are ineffective and misaligned with the local context, traditional knowledge systems, and specific community concerns, therefore marginalizing local actors from meaningful participation. The translation of climate communication into climate change action is challenging for local actors due to prevailing exclusion from discussion and a lack of engagement, which contributes to misunderstanding and poor climate change action. The study indicates that enhancing climate change communication in Nigeria necessitates the development of integrative strategies tailored to the language, cultural, and educational context that will encourage the local actors to participate effectively in this discussion. The paper recommends translating information into local languages and integrating local proverbs and mythological interpretations that can be positively employed to combat climate change within these communities more organically. Full article

Background: Bilio-pancreatic tumors are a severe form of cancer with a high rate of associated mortality. These patients showed the presence of bacteria such as Escherichia coli and Pseudomonas spp. in the bile-pancreatic tract. Therefore, efficient antibiotic therapy is essential to reduce bacterial resistance and adverse events in cancer patients. Recent studies on the seasonality of infectious diseases may aid in developing effective preventive measures. This study examines the seasonal impact on the bile microbiota composition and the antibiotic resistance of its microorganisms in patients with hepato-pancreatic-biliary cancer. Methods: We retrospectively evaluated the effect of the seasonally from 149 strains isolated by 90 Italian patients with a positive culture of bile samples collected through endoscopic retrograde cholangiopancreatography between 2010 and 2020. Results: Across all seasons, the most frequently found bacteria were E. coli, Pseudomonas spp., and Enterococcus spp. Regarding antibiotic resistance, bacteria showed the highest resistance to 3GC, fluoroquinolones, aminoglycosides, fosfomycin, and piperacillin-tazobactam in the summer and the lowest resistance in the spring, except for carbapenems and colistin. Conclusions: Antibiotic resistance has negative effects in cancer patients who rely on antibiotics to prevent and treat infections. Knowing whether bacterial and fungal resistance changes with the seasons is key information to define adequate and more effective antibiotic therapy. Full article

The job values of college graduates are rapidly changing, but a mismatch between industry expectations and young chefs’ values has emerged. To capture the heterogeneity in job values that traditional variable-centered approaches may overlook, this study employed Latent Profile Analysis, a person-centered method, to classify the job value profiles of culinary arts graduates and examine their impact on major–job match and subjective well-being. A total of 386 culinary arts graduates, extracted from the Graduates Occupational Mobility Survey, were classified into six latent profiles. First, the most prevalent profile (Profile 4) emphasized environmental and developmental values, and was associated with a higher proportion of women and a greater likelihood of unemployment. Second, graduates who valued job attributes across all dimensions (Profile 1) were more likely to secure employment in or outside their field than those in Profiles 2, 3, and 5 were. Third, negative emotions increased the likelihood of belonging to Profiles 2, 3, and 5 compared to Profile 1. Finally, higher life satisfaction reduced the probability of belonging to Profiles 4, 5, or 6 compared to Profile 1. These findings emphasize the importance of aligning HR policies with graduates’ job values. Such alignment can enhance employment within graduates’ academic disciplines and improve their subjective well-being. Full article

By comparing two significant characters in a Biblical and a Confucian story, respectively, this article examines how the two traditions referred to share a common understanding of what “reconciliation” is meant to be. I compare Joseph in Genesis and Shun in the Mencius, focusing on how their crying contributes to familial reconciliation. The comparison raises anthropological commonalities between these narratives concerning structures of violence and the process of personal transformation leading to interpersonal reconciliation. There is particular emphasis on the significance of emotions: the way in which tearful emotions are expressed and perceived functions either as cause (Shun) or effect (Joseph) of the aggressor’s transformation, thus triggering the reconciliatory process. Following the suggested interpretation of these narratives as historic encounters between cultures of different provenance, the commonalities found in both approaches to reconciliation can potentially serve as a source of inspiration for present-day relations between religions and civilizations. Full article

Hematological malignancies originating from blood, bone marrow, and lymph nodes include leukemia, lymphoma, and myeloma, which necessitate the use of a distinct chemotherapeutic approach. Drug resistance frequently complicates their treatment, highlighting the need for predictive tools to guide therapeutic decisions. Conventional 2D/3D cell cultures do not fully encompass in vivo criteria, and translating disease models from mice to humans proves challenging. Organ-on-a-chip technology presents an avenue to surmount genetic disparities between species, offering precise design, concurrent manipulation of various cell types, and extrapolation of data to human physiology. The development of bone-on-a-chip (BoC) systems is crucial for accurately representing the in vivo bone microenvironment, predicting drug responses for hematological cancers, mitigating drug resistance, and facilitating personalized therapeutic interventions. BoC systems for modeling hematological cancers and drug research can encompass intricate designs and integrated platforms for analyzing drug response data to simulate disease scenarios. This review provides a comprehensive examination of BoC systems applicable to modeling hematological cancers and visualizing drug responses within the intricate context of bone. It thoroughly discusses the materials pertinent to BoC systems, suitable in vitro techniques, the predictive capabilities of BoC systems in clinical settings, and their potential for commercialization. Full article

Medulloblastoma (MB) groups 3 and 4 lack targeted therapies despite their dismal prognoses. Ion channels and pumps have been implicated in promoting MB metastasis and growth; however, their roles remain poorly understood. In this study, we repurposed FDA-approved channel blockers and modulators to investigate their potential anti-tumor effects in MB cell lines (DAOY and D283) and primary cell cultures derived from a patient with MB. For the first time, we report spontaneous calcium signaling in MB cells. Spontaneous calcium signals were significantly reduced by mibefradil (calcium channel blocker), paxilline (calcium-activated potassium channel blocker), and thioridazine (potassium channel blocker). These drugs induced dose-dependent cytotoxicity in both the DAOY and D283 cell lines, as well as in primary cell cultures of a patient with group 3 or 4 MB. In contrast, digoxin and ouabain, inhibitors of the Na/K pump, reduced the calcium signaling by over 90% in DAOY cells and induced approximately 90% cell death in DAOY cells and 80% cell death in D283 cells. However, these effects were significantly diminished in the cells derived from a patient with MB, highlighting the variability in drug sensitivity among MB models. These findings demonstrate that calcium signaling is critical for MB cell survival and that the targeted inhibition of calcium pathways suppresses tumor cell growth across multiple MB models. Full article

This study compared two types of automatic milking systems (AMSs) and their relationship with epidemiological indices of subclinical mastitis (SCM) and prevalence of mastitis-causing pathogens. Conducted between 2020 and 2023 on a dairy farm in Vacaria, Rio Grande do Sul, Brazil, this study analyzed data from 464 lactating cows housed in compost-bedded pack barns (CBPBs) and milked by eight AMS units: four from DeLaval (which utilizes teat cup for teat cleaning) and four from Lely (which utilizes brushes for teat cleaning). SCM incidence, prevalence, percentage of chronic, and cured cows were determined using somatic cell counts (SCCs) and microbiological cultures. Statistical analyses included the Wilcoxon signed-rank test and Chi-square test to evaluate SCM indices and pathogen associations with AMSs. No significant difference was observed in SCM prevalence (p = 0.3371), percentage of chronic (p = 0.3590) and cured cows (p = 0.4038), SCC (p = 0.1290), and total bacterial count (TBC) (p = 0.8750) between AMS types. However, the SCM incidence was higher in the Lely (14.7%) than in the DeLaval AMS (9.1%) (p = 0.0032). The Chi-square results revealed that the Lely AMS was associated with major pathogens like Staphylococcus aureus and Escherichia coli, whereas DeLaval showed associations with minor environmental and contagious pathogens, particularly non-aureus Staphylococci. The findings indicate a relationship between AMS-cleaning systems and pathogen spread, suggesting that Lely AMS may contribute to more aggressive infections due to its cleaning system. Full article

Background/Objectives: Physical activity (PA) and exercise have demonstrated numerous benefits for patients with cancer. However, there may be different barriers which vary according to geographical area. The aim of this study was to compare oncologists, PA recommendations, PA patterns and barriers in two different geographical areas. Methods: A total of 254 patients were included, 239 of them women and 15 of them men, while 41.3% of the sample was Polish and 58.7% of the sample Spanish. Results: In terms of differences in time spent on PA per day, the Spanish spent more time walking than the Polish did (p = 0.007). However, no significant differences were found between countries, types or intensity of exercise, and there was a significant relationship between age and type of PA modality (p = 0.002). At the same time, there were different reasons for not practicing PA (p = 0.009). The subsequent analysis showed that younger adults were prone to more vigorous-intensity exercise than the other age groups (p = 0.001, η² = 0.08). Furthermore, there was a significant difference between age groups, countries and sitting time (p = 0.01), with Polish patients spending more time sitting than Spaniards (p = 0.01, η² = 0.06). Conclusions: Although PA patterns in the two countries were similar, the main barriers to exercise differed. Therefore, PA programs should be as personalized as possible (taking into account sociodemographic, cultural and climatic characteristics). Ultimately, to improve the quality of life and health of their patients, oncologists should provide those activities that are most beneficial to their patients. Full article

Palm groves are abundant in southeastern Mexico and have significant economic and socio-cultural relevance. Studies on the production and management of palm groves are abundant in the area. However, an ecological approach is scarce but necessary for conserving these overexploited species. Palm groves are abundant on the island of Cozumel and develop on contrasting substrates: rocky and sandy ones. Thus, we aimed to explore how soil types affected community structure and composition. We sampled a total of 2900 m² (29 plots of 10 × 10 m) of palm grove, 13 growing in sandy soil and 16 in rocky soil. We registered the species present in each plot, plant density, cover, height, and DBH. A hierarchical cluster analysis re-grouped the sampled plots based on their floristic affinities, and thus, we had 17 plots for the sandy substrate and 12 from the rocky substrate, which were used for later analyses. The dominant species in both palm groves was Thrinax radiata, and species richness was the same in both soil types (33 species). Contrasting substrates resulted in different community composition and structure. The palm groves from the sandy substrate had more vines but fewer shrubs and higher plant density, height, and DBH. The above suggests that the low availability of soil in the rocky substrate does not allow the same state of vegetation development that occurs in the sandy substrate. Full article

Edible insects constitute a healthy food source providing a sustainable alternative to traditional animal protein. The present study explored consumers’ perceptions and attitudes toward insect consumption, and defined the main motivational factors influencing public awareness and acceptance toward entomophagy. Using a qualitative research design, individual-level data were selected from a sample of 70 consumers in Greece via semi-structured personal in-depth interviews. The Grounded Theory framework was adopted to develop awareness, perception and acceptance drivers. Although the participants were knowledgeable about the usage of insects as food, the great majority demonstrated abhorrence toward entomophagy, describing feelings of disgust and repulsion. Furthermore, the respondents seemed to be reluctant towards the distribution and availability of edible insects and insect-based food options in consumer markets, whereas food safety concerns were strong as many consumers seemed to question the relevant preparation regulations. A lack of information and cultural influences were found to restrict consumers’ acceptance of entomophagy, whereas health and food safety concerns comprised an inhibiting factor in incorporating edible insects in Greek cuisine. This study emphasized the need for a holistic information plan, which will help both food businesses and consumers understand the vital role of edible insects in modern food environments. Full article

Selenium–polysaccharides possess antioxidant properties, making them promising materials for functional foods, pharmaceuticals, and clinical applications. This study examines the incorporation of selenium into polysaccharides via mycelial biosynthesis and its effects on structure and antioxidant activity. Polysaccharides obtained from Lentinula edodes-submerged cultures grown in Se-supplemented and non-supplemented media were analysed for Se content (RP-HPLC/FLD), structure (FT-IR, HPLC, and HPGPC-ELSD), and antioxidant activity (DPPH scavenging, reducing power, and Fe²⁺ chelation). Two low-molecular-weight Se–heteropolysaccharides (Se-FE-1.1 and Se-FE-1.2) containing ~80 and 125 µg/g Se were isolated, primarily composed of glucose, mannose, and galactose with β-glycosidic linkages. Se incorporation into polysaccharides selectively enhanced their antioxidant activity in the DPPH radical scavenging assay, with minimal effects observed in iron chelation and reducing power assays. Crude Se–polysaccharides displayed the highest antioxidant activity, suggesting an additional contribution from protein components. Our findings demonstrate that Se is effectively incorporated into polysaccharides, altering monosaccharide composition while preserving glycosidic linkages. The selective enhancement of radical scavenging suggests that selenium plays a specific role in antioxidant activity, primarily influencing radical scavenging mechanisms rather than interactions with metal ions. Further research is needed to clarify the mechanisms of selenium incorporation, the nature of its bonding within the polysaccharide molecule, and its impact on biological activity. Full article