Search Results (17,797)

Corrosion in the sheets produced leads to significant material losses, including the loss of resources, capital, labor, energy and knowledge. Corrosion control is significant for sheets produced and sent to customers in iron and steel factories. Surface corrosion testing of produced sheets and the accurate detection of corrosion levels are of great importance. The corrosion detection process for sheets in steel factories is performed visually with the naked eye. This is a subjective and time-consuming method. Identifying corrosion damage by visual detection and accurately determining the type and extent of corrosion requires expertise. Wrong decisions at this stage lead to losses during the production phase. Therefore, there is a need for systems that can automate this process and make it human-independent. In this study, a decision support system was designed to automatically detect the level of corrosion in galvanized sheets using convolutional neural networks. The average accuracy of the system is 97.5%, the average precision is 0.98, the average recall is 1 and the average F1 score is 0.99. The results we obtained indicate that a successful system has been developed for the detection and determination of corrosion levels. The high performance of the convolutional neural network models used for corrosion detection supports the practical applicability of the developed system. This system will increase the reliability and efficiency of industrial processes by enabling the accurate and automatic classification of corrosion. This system, which meets a significant need in this area for industrial organizations, reduces production costs and also makes the corrosion detection process more consistent and faster. Full article

In this work, a microfluidic paper-based analytical device (µPAD) for simultaneous detection of Fe, Zn, and Mn ions using immobilized chromogenic reagents Ferene S, xylenol orange, and 1-(2-pyridylazo)-2-naphthol, respectively, is presented. As the effective recognition of analytes via respective chromogens takes place under extremely different pH conditions, experiments reported in this publication are focused on optimization of the µPAD architecture allowing for the elimination of potential cross effects. The paper-based microfluidic device was fabricated using low-cost and well-reproducible wax-printing technology. For optical detection of color changes, an ordinary office scanner and self-made RGB-data processing program were applied. Optimized and stable over time, µPADs allow fast, selective, and reproducible multianalyte determinations at submillimolar levels of respective heavy metal ions, which was confirmed by results of the analysis of solutions mimicking real samples of wastewater. The presented concept of simultaneous determination of different analytes that required extremely different conditions for detection can be useful for the development of other multianalyte microfluidic paper-based devices in the µPAD format. Full article

5-Aminolevulinic acid (5-ALA), a non-proteinogenic amino acid, is an intermediate in the biosynthesis of heme and exerts antiviral effects against feline coronavirus (FCoV); however, the underlying mechanisms remain unclear. In the biosynthesis of heme, 5-ALA is condensed and converted to protoporphyrin IX (PpIX), which is then transformed into heme by the insertion of ferrous iron. Previous research has suggested that the metabolites generated during heme biosynthesis contribute to the antiviral effects of 5-ALA. Therefore, the present study investigated the in vitro mechanisms responsible for the antiviral effects of 5-ALA. The results obtained revealed that 5-ALA and PpIX both effectively reduced the viral titer in the supernatant of FCoV-infected fcwf-4 cells. Moreover, PpIX exerted virucidal effects against FCoV. We also confirmed that 5-ALA increased PpIX levels in cells. While hemin induced heme oxygenase-1 gene expression, it did not reduce the viral titer in the supernatant. Sodium ferrous citrate decreased PpIX levels and suppressed the antiviral effects of 5-ALA. Collectively, these results suggest that the antiviral effects of 5-ALA against FCoV are dependent on PpIX. Full article

Various green materials like biochar and Fe⁰ (nano-scale zerovalent iron, nZVI) have been applied to remediate aqueous Cr(VI) contamination, but few studies have tried to further improve the performance of nZVI and/or biochar composites with different sulfidation methods. Here, we modified a hybrid material of nZVI@biochar with Na₂S and pyrite (FeS₂), applied it to remove aqueous Cr(VI) under different experimental conditions, and revealed key factors influencing Cr(VI) removal performance. The results show that pyrite loading is an effective sulfidation method to increase the Fe and S contents in composites. FeS_x-nZVI@BC (1:1) had a Cr(VI) removal efficiency of ~95% with 5 mg/L Cr(VI) loaded, which was much higher than other hybrid composites. The Cr(VI) removal efficiency of FeSx-nZVI@BC showed a decreasing trend under pH conditions that increased from pH 3 to pH 9. The presence of dissolved oxygen and aqueous Cu²⁺ and Cd²⁺ could significantly suppress the removal of aqueous Cr(VI), while humic acids at different concentrations did not suppress Cr(VI) removal. After the reaction, it was observed with an energy-dispersive spectrometer (SEM-EDS) that most Cr in the solid phase was closely associated with pyrite minerals. X-ray photoelectron spectroscopy (XPS) spectra, together with the Fe²⁺-quenching method, confirmed that Fe (Fe²⁺ or Fe⁰) acted as the main electron donor, contributing to ~90% of the Cr(VI) reduction. Our study indicates that pyrite loading could further improve the performance of remediation materials and that the pyrite-loaded nZVI@BC composite is a green material with strong potential to be applied in the remediation of water contaminated by Cr(VI). Full article

Background/Objectives: Homocysteine (Hcy) and iron are factors co-related with the progression of cardiovascular diseases. The vascular endothelium is an important barrier for physiological homeostasis, and its impairment initiates cardiovascular injury. However, the mechanism underlying Hcy-caused vascular endothelial cell injury and the participation of iron are not fully elucidated. This study aims to investigate the Hcy-induced vascular endothelial injury and iron metabolism dysfunction as well as the underlying molecular mechanism. Methods: Human umbilical vein endothelial cells (HUVECs) were employed as the experimental model to examine the Hcy-induced endothelial injury and its underlying mechanism via various biochemical assays. Results: Hcy suppressed the cell viability and proliferation and caused cell death in a concentration-dependent manner. Hcy induced cell cycle arrest, apoptosis, and autophagy as well as impairment of intracellular energy metabolism. Hcy disrupted the intracellular antioxidant system and mitochondrial function by increasing intracellular ROS, MDA and mitochondrial content, and decreasing the SOD activity and mitochondrial membrane potential. Hcy significantly reduced the GSH-Px activity along with the accumulation of intracellular GSH in a concentration-dependent manner. Ferroptosis inhibitors, Ferrostatin-1 (Fer-1), and Deferoxamine (DFO) significantly decreased the Hcy-caused cytotoxicity accompanied by a reduction in dysregulated mitochondria content, but only DFO ameliorated the elevation of intracellular ROS, and neither Fer-1 nor DFO affected the Hcy-caused reduction in intracellular ATP. In addition, Hcy decreased the intracellular concentration of iron, and supplementing Hcy with various concentrations of Fe³⁺ increased the cell viability and decreased the LDH release in a concentration-dependent manner. Hcy dramatically decreased the mRNA expression level of transferrin receptor while increasing the mRNA expression levels of transferrin, ferritin light chain, ferritin heavy chain, ferroportin, and SLC7A11. Moreover, Hcy suppressed the protein expression of phosphor-Akt, phosphor-mTOR, Beclin-1, LC3A/LC3B, Nrf2, HO-1, phosphor-MEK1/2, phosphor-ERK1/2, and Caspase-3 in concentration- and time-dependent manners. Conclusions: Hcy-induced vascular endothelial injury is likely to be associated with apoptosis and autophagy, but not ferroptosis. The key underlying mechanisms are involved in the disruption of the intracellular antioxidant system and iron metabolism via regulation of PI3K/Akt/mTOR, MAPKs, Nrf2/HO-1, and iron metabolism. Full article

To determine the etiological agents responsible for acute pneumonia in puppies in China, this study utilized bronchoalveolar lavage (BAL) fluid extraction to enable the isolation, culture, biochemical identification, and 16S rRNA PCR amplification of the pathogens. Following preliminary identification, the pathogens underwent analysis for antibiotic resistance phenotypes and resistance genes. Additionally, the study examined the presence of virulence genes, conducted multilocus sequence typing (MLST), and performed whole-genome sequencing (WGS). The findings revealed that all four isolated pathogens were characterized as extraintestinal pathogenic Escherichia coli (ExPEC). The examined ExPEC strains demonstrated resistance to cephalosporins, tetracyclines, and penicillins, while remaining susceptible to aminoglycosides, beta-lactamase inhibitors, carbapenems, chloramphenicols, and sulfonamides. An analysis of virulence genes identified the presence of eight genes, namely CNF-I, fyuA, fimC, papC, ompA, fimH, irp2, and iroN, which are implicated in their invasiveness and potential to inflict tissue damage. The MLST analysis revealed that all ExPEC strains were classified under either sequence type ST131 (Achtman database) or ST43 (Pasteur database). The study further determined that these strains were absent in the kennel’s drinking water source, thereby ruling out water contamination as a potential factor in the emergence of ST131-type ExPEC. This study offers a theoretical framework and empirical evidence for elucidating the potential pathogenic mechanisms and clinical therapeutic strategies of ExPEC in the etiology of acute pneumonia in puppies. Full article

The use of food supplements (FSs) is becoming an increasingly common trend observed in society. This is related to frequently observed nutritional deficiencies and the inability to provide sufficient amounts of nutrients, including vitamins and minerals, through the diet. The ease of registering FSs sometimes results in low-quality preparations on the market. Therefore, our research aimed to assess the content of one of the most popular trace element components, iron (Fe), in FSs available in Poland. This study covered 109 preparations purchased from stationary pharmacies and online pharmacies. The following criteria were used to characterize FSs in the data analysis: the Fe content declared by the manufacturer, pharmaceutical form, type of Fe salt, manufacturer’s country of origin, contents of other trace elements or minerals, presence of additional ingredients, age of the target group, and addition of vitamins B2, B6, B12, and C. The Fe content was quantified using atomic absorption spectrometry after mineralization using microwaves. It was demonstrated that 69.73% of the preparations contained more Fe than the value declared by the manufacturer (and corrected for permissible deviations), 11.00% contained less Fe than declared, and only 19.27% were within the norm. In summary, the FS market requires the improvement of manufacturing processes and increased control, which should translate into patient safety. Full article

Background: Iron deficiency (ID) remains the leading cause of anemia, affects a vast number of persons globally, and continues to be a significant global health burden. Comprehending the patterns of ID burden is essential for developing targeted public health policies. Methods: Using data from the Global Burden of Disease (GBD) 2021 study for the years 1990–2021, the XGBoost model was constructed to predict prevalence and disability-adjusted life years (DALYs) for the period 2022–2050, based on key demographic variables. Shapley Additive exPlanations (SHAP) values were applied to interpret the contributions of each variable to the model’s predictions. Additionally, the Age–Period–Cohort (APC) model was used to evaluate the effects of age, period, and birth cohort on both prevalence and DALYs. The relationship between the Socio-Demographic Index (SDI) and ID’s age-standardized prevalence rate (ASPR) as well as the age-standardized DALYs rate (ASDR) was also analyzed to assess the influence of socioeconomic development on disease burden. Results: The global prevalent cases of ID grew from 984.61 million in 1990 to 1270.64 million in 2021 and are projected to reach 1439.99 million by 2050. Similarly, global DALYs from ID increased from 28.41 million in 1990 to 32.32 million in 2021, with a projected rise to 36.13 million by 2050. The ASPR declined from 18,204/100,000 in 1990 to 16,433/100,000 in 2021, with an estimated annual percentage change (EAPC) of −0.36% over this period. It is expected to decrease further to 15,922 by 2050, with an EAPC of −0.09% between 2021 and 2050. The ASDR was 518/100,000 in 1990 and 424/100,000 in 2021, with an EAPC of −0.68% from 1990 to 2021. It is expected to remain relatively stable at 419/100,000 by 2050, with an EAPC of −0.02% between 2021 and 2050. In 2021, the highest ASPRs were recorded in Senegal (34,421/100,000), Mali (34,233/100,000), and Pakistan (33,942/100,000). By 2050, Mali (35,070/100,000), Senegal (34,132/100,000), and Zambia (33,149/100,000) are projected to lead. For ASDR, Yemen (1405/100,000), Mozambique (1149/100,000), and Mali (1093/100,000) had the highest rates in 2021. By 2050, Yemen (1388/100,000), Mali (1181/100,000), and Mozambique (1177/100,000) are expected to remain the highest. SHAP values demonstrated that gender was the leading predictor of ID, with age and year showing negative contributions. Females aged 10 to 60 consistently showed higher prevalence and DALYs rates compared to males, with the under-5 age group having the highest rates for both. Additionally, men aged 80 and above exhibited a rapid increase in prevalence. Furthermore, the ASPR and ASDR were significantly higher in regions with a lower SDI, highlighting the greater burden of ID in less developed regions. Conclusions: ID remains a significant global health concern, with its burden projected to persist through 2050, particularly in lower-SDI regions. Despite declines in ASPR and ASDR, total cases and DALYs are expected to rise. SHAP analysis revealed that gender had the greatest influence on the model’s predictions, while both age and year showed overall negative contributions to ID risk. Children under 5, women under 60, and elderly men aged 80+ were the most vulnerable groups. These findings underscore the need for targeted interventions, such as improved nutrition, early screening, and addressing socioeconomic drivers through iron supplementation programs in low-SDI regions. Full article

Jeju Island, located in the northern East China Sea, is experiencing a rapid rise in water temperature due to climate change. This has led to the increased activity of subtropical species and extreme fluctuations in coastal ecosystems, such as macroalgal blooms and coral bleaching. Additionally, the region is experiencing more frequent and intense typhoons. This study investigated the green tides caused by Ulva, particularly Ulva ohnoi, a subtropical species, and the effects of typhoons on these blooms through photographic analysis of the Jeju coastline. The study area was consistently covered by Ulva species every August from 2020 to 2022. Super typhoons struck Jeju Island every September during the study period, with wind speeds exceeding 20 m/s. In 2020 and 2022, the green tides largely dissipated following the typhoons. This ironic outcome highlights how climate-driven increases in subtropical Ulva biomass are being mitigated by the increasing frequency of super typhoons. However, despite the impact of super typhoon Chanthu in September 2021, there was no significant reduction in the Ulva bloom area. This anomaly may be attributable to the dominant easterly wind system in 2021, as the study area faces east, preventing the typhoon from influencing the distribution of Ulva blooms. These findings suggest that the wind intensity and direction of annual typhoons play a critical role in determining the resolution of green tide outbreaks. Full article

Metabolic syndrome (MetS) is the most common adverse drug reaction from psychiatric pharmacotherapy. Neuroreceptor blockade by the antipsychotic drug clozapine induces MetS in about 30% of patients. Similar to insulin resistance, clozapine impedes Akt kinase activation, leading to intracellular glucose and glutathione depletion. Additional cystine shortage triggers tryptophan degradation to kynurenine, which is a well-known AhR ligand. Ligand-bound AhR downregulates the intracellular iron pool, thereby increasing the risk of mitochondrial dysfunction. Scavenging iron stabilizes the transcription factor HIF-1, which shifts the metabolism toward transient glycolysis. Furthermore, the AhR inhibits AMPK activation, leading to obesity and liver steatosis. Increasing glucose uptake by AMPK activation prevents dyslipidemia and liver damage and, therefore, reduces the risk of MetS. In line with the in vitro results, feeding experiments with rats revealed a disturbed glucose-/lipid-/iron-metabolism from clozapine treatment with hyperglycemia and hepatic iron deposits in female rats and steatosis and anemia in male animals. Decreased energy expenditure from clozapine treatment seems to be the cause of the fast weight gain in the first weeks of treatment. In patients, this weight gain due to neuroleptic treatment correlates with an improvement in psychotic syndromes and can even be used to anticipate the therapeutic effect of the treatment. Full article

The interaction of nano-fertilizers with commercially important crops can be a promising solution to increase both crop yield and quality. This study investigated the effect of iron oxide nanoparticles (Fe₃O₄ NPs) on four-week-old garden cress (Lepidium sativum L.) seedlings. Iron is an essential micronutrient for plants but is not always available in sufficient quantities, which can lead to chlorosis and even plant death. The seedlings were grown hydroponically, with three concentrations (1 mg/L, 5 mg/L, and 10 mg/L) of the NPs, alongside a control group with no additions. During the experiment, the following methods were employed: measurement of stem and root length, spectrophotometry to determine chlorophyll absorbance and concentration, and the RAPD technique to assess the genotoxicity of Fe₃O₄ NPs. The study demonstrated a significant increase in the shoot length of cress at all concentrations compared to the control group (p < 0.05; p < 0.01). The light absorption and chlorophyll concentration levels in the experimental groups significantly increased compared to the control group (p < 0.001). Genotoxicity analysis revealed that the genotoxic impact of the NPs on the garden cress genome was only 10%, a statistically insignificant level. The findings suggest that Fe₃O₄ NPs exhibit low genotoxicity and have the potential to enhance the growth and chlorophyll content of cress seedlings in hydroponic conditions. Full article

Traditionally, acoustic methods for leak inspection are based on the measurement of the acceleration of the external pipe wall or of the acoustic pressure in the pipe. This work presents an alternative inspection methodology based on measuring the acoustic velocity vector in the fluid filling the pipe. Unlike the acoustic pressure, the acoustic quantity is very sensitive to the presence of a pipe wall defect. Such defects are important to detect before they develop into leaks, which can lead to the loss of water, environmental pollution and service disruption. A new sensor design is proposed to measure the acoustic velocity vector in a pipe. A model is presented to demonstrate the underpinning theory behind this new sensor technology. The results of this model are compared with experimental data based on measurements of the acoustic velocity in an exhumed section of ductile iron pipe. These sensors can be deployed on robots to autonomously monitor the deterioration of buried pipes to support proactive asset management at a low operational cost. Full article

It is very important to clarify the optimization method of the rock-like material ratio for accurately characterizing mechanical properties similar to the original rock. In order to explore the optimal ratio of rock-like materials in gneissic granite, the water–paste ratio, iron powder content and coarse sand content were selected as the influencing factors of the ratio. An orthogonal test design and sensitivity analysis of variance were used to obtain the significant influencing factors of the ratio factors on seven macroscopic mechanical parameters, including compressive strength σ_c, tensile strength σ_t, shear strength τ_f, elastic modulus E, Poisson’s ratio ν, internal friction angle φ and cohesion c. A multivariate linear regression equation was constructed to obtain the quantitative relationship between the significant ratio factors and the macroscopic mechanical parameters. Finally, a rock-like material ratio optimization program based on genetic algorithm inversion was written. The results show that the water–paste ratio had extremely significant effects on σ_c, σ_t, τ_f, E, ν and c. The iron powder content had a highly significant effect on σ_c, σ_t, τ_f and c, and it had a significant effect on ν and φ. Coarse sand content had a significant effect on σ_c, E and c. The multiple linear regression model has good reliability after testing, which can provide theoretical support for predicting the macroscopic mechanical parameters of rock-like materials to a certain extent. After testing, the ratio optimization program works well. When the water–paste ratio is 0.5325, the iron powder content is 3.975% and the coarse sand content is 15.967%, it is the optimal ratio of rock-like materials. Full article

One of the major global health threats in the present era is antibiotic resistance. Biosynthesized iron oxide nanoparticles (FeNPs) can combat microbial infections and can be synthesized without harmful chemicals. In the present investigation, 16S rRNA gene sequencing was used to discover Streptomyces sp. SMGL39, an actinomycete isolate utilized to reduce ferrous sulfate heptahydrate (FeSO₄.7H₂O) to biosynthesize FeNPs, which were then characterized using UV–Vis, XRD, FTIR, and TEM analyses. Furthermore, in our current study, the biosynthesized FeNPs were tested for antimicrobial and antibiofilm characteristics against different Gram-negative, Gram-positive, and fungal strains. Additionally, our work examines the biosynthesized FeNPs’ molecular docking and binding affinity to key enzymes, which contributed to bacterial infection cooperation via quorum sensing (QS) processes. A bright yellow to dark brown color shift indicated the production of FeNPs, which have polydispersed forms with particle sizes ranging from 80 to 180 nm and UV absorbance ranging from 220 to 280 nm. Biosynthesized FeNPs from actinobacteria significantly reduced the microbial growth of Fusarium oxysporum and L. monocytogenes, while they showed weak antimicrobial activity against P. aeruginosa and no activity against E. coli, MRSA, or Aspergillus niger. On the other hand, biosynthesized FeNPs showed strong antibiofilm activity against P. aeruginosa while showing mild and weak activity against B. subtilis and E. coli, respectively. The collaboration of biosynthesized FeNPs and key enzymes for bacterial infection exhibits hydrophobic and/or hydrogen bonding, according to this research. These results show that actinobacteria-biosynthesized FeNPs prevent biofilm development in bacteria. Full article

Amaranthus cruentus L. (varieties: Juana, Aurelia, Elena) and Amaranthus viridis L. (variety: Callaloo) have long been utilized in food products for human consumption in Central and South America. However, there is limited information on the chemical composition of these species’ leaves and grains grown in Puerto Rico. This study aimed to fill this gap by evaluating the nutritional profile of these four amaranth varieties cultivated in Puerto Rico. A compositional analysis was conducted using official methods, focusing on lysine, protein, dietary fiber, and mineral content. The results showed high lysine content in both species. Significant differences (p < 0.05) were found in crude protein levels among the leaves, with Elena (23%) and Aurelia (21%) showing the highest values. While protein content among grains averaged 19%, there were no significant differences between varieties. The analysis of dietary fiber revealed significant differences (p < 0.05) in insoluble dietary fiber (IDF) and total dietary fiber (TDF) for the leaves and in IDF, soluble dietary fiber (SDF), and TDF for the grains. Additionally, calcium, magnesium, and phosphorus concentrations differed significantly (p < 0.05) in the leaves, while iron, potassium, and zinc showed no significant variation. Significant differences were found in the grains for calcium, magnesium, iron, and phosphorus. This research highlights the excellent nutritional value of amaranth leaves and grains grown in Puerto Rico, with Elena and Aurelia having exceptionally high protein content in their leaves. Full article