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Search Results (12,106)

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14 pages, 2703 KiB  
Article
Modulating Near-Infrared Persistent Luminescence via Diverse Preparation Approaches
by Xiaomeng Wang, Hengli Zhu, Yan Liu, Jingyuan Li, Lejia Cao, Jiaren Du and Hengwei Lin
Nanomaterials 2024, 14(19), 1613; https://doi.org/10.3390/nano14191613 - 9 Oct 2024
Abstract
Near-infrared (NIR) persistent luminescence (PersL) materials have attracted extensive attention due to their great promise in medical diagnostics, bio-imaging, night vision surveillance, multi-level anticounterfeiting, and information encryption. To achieve NIR PersL (micro/nano-) materials with the desired properties, a variety of synthesis methods have [...] Read more.
Near-infrared (NIR) persistent luminescence (PersL) materials have attracted extensive attention due to their great promise in medical diagnostics, bio-imaging, night vision surveillance, multi-level anticounterfeiting, and information encryption. To achieve NIR PersL (micro/nano-) materials with the desired properties, a variety of synthesis methods have been employed, including solid-phase reaction and liquid-phase synthesis. Different synthesis methods have different but important effects on the micro/nano-structure, luminescence, and PersL properties of the materials. Moreover, the influence of various synthesis methods on the properties of NIR PersL materials determines the selection of preparation approaches for other new material systems. Taking the representative NIR PersL ZnGa2O4:Cr3+ material as an example, four synthesis procedures are applied, namely, high-temperature solid-state reaction (SSR), high-temperature molten salt method (MSM), hydrothermal method (HM), and microwave-assisted solid-state (MASS) method. The structural and luminescent properties of samples made by SSR, MSM, HM, and MASS are compared. Notably, it is revealed that the MASS method can create additional trapping energy levels, which is of great significance for emerging applications. This work demonstrates the different effects of synthesis methods on PersL performance and provides a good guideline for the rapid and reasonable selection of preparation methods for diverse applications. Full article
21 pages, 1401 KiB  
Review
Mental Imagery between Cognition and Emotion: A Narrative Review
by Francesco Maria Boccaccio, Alessia Pennisi, Claudia Savia Guerrera, Giuseppe Alessio Platania, Vittoria Torre, Simone Varrasi, Venera Francesca Vezzosi, Flavia Coco, Sabrina Castellano and Concetta Pirrone
Psychiatry Int. 2024, 5(4), 697-717; https://doi.org/10.3390/psychiatryint5040049 (registering DOI) - 9 Oct 2024
Abstract
Mental imagery is a cognitive ability that enables individuals to simulate sensory experiences without external stimuli. This complex process involves generating, manipulating, and experiencing sensory perceptions. Despite longstanding interest, understanding its relationship with other cognitive functions and emotions remains limited. This narrative review [...] Read more.
Mental imagery is a cognitive ability that enables individuals to simulate sensory experiences without external stimuli. This complex process involves generating, manipulating, and experiencing sensory perceptions. Despite longstanding interest, understanding its relationship with other cognitive functions and emotions remains limited. This narrative review aims to address this gap by exploring mental imagery’s associations with cognitive and emotional processes. It emphasizes the significant role of mental imagery on different cognitive functions, with a particular focus on learning processes in different contexts, such as school career, motor skill acquisition, and rehabilitation. Moreover, it delves into the intricate connection between mental imagery and emotions, highlighting its implications in psychopathology and therapeutic interventions. The review also proposes a comprehensive psychometric protocol to assess mental imagery’s cognitive and emotional dimensions, enabling a thorough evaluation of this complex construct. Through a holistic understanding of mental imagery, integrating cognitive and emotional aspects, researchers can advance comprehension and application in both research and clinical settings. Full article
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17 pages, 2983 KiB  
Article
Pose Estimation of a Cobot Implemented on a Small AI-Powered Computing System and a Stereo Camera for Precision Evaluation
by Marco-Antonio Cabrera-Rufino, Juan-Manuel Ramos-Arreguín, Marco-Antonio Aceves-Fernandez, Efren Gorrostieta-Hurtado, Jesus-Carlos Pedraza-Ortega and Juvenal Rodríguez-Resendiz
Biomimetics 2024, 9(10), 610; https://doi.org/10.3390/biomimetics9100610 - 9 Oct 2024
Abstract
The precision of robotic manipulators in the industrial or medical field is very important, especially when it comes to repetitive or exhaustive tasks. Geometric deformations are the most common in this field. For this reason, new robotic vision techniques have been proposed, including [...] Read more.
The precision of robotic manipulators in the industrial or medical field is very important, especially when it comes to repetitive or exhaustive tasks. Geometric deformations are the most common in this field. For this reason, new robotic vision techniques have been proposed, including 3D methods that made it possible to determine the geometric distances between the parts of a robotic manipulator. The aim of this work is to measure the angular position of a robotic arm with six degrees of freedom. For this purpose, a stereo camera and a convolutional neural network algorithm are used to reduce the degradation of precision caused by geometric errors. This method is not intended to replace encoders, but to enhance accuracy by compensating for degradation through an intelligent visual measurement system. The camera is tested and the accuracy is about one millimeter. The implementation of this method leads to better results than traditional and simple neural network methods. Full article
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14 pages, 6074 KiB  
Article
Members of WRKY Group III Transcription Factors Are Important in Mite Infestation in Strawberry (Fragaria × ananassa Duch.)
by Peng Chen, Xianhong Zhou, Haiting Wang, Xiuxia Zhang, Lei Wang, Huanhuan Gao, Qianying Zhuang, Heqin Li and Ansheng Zhang
Plants 2024, 13(19), 2822; https://doi.org/10.3390/plants13192822 - 9 Oct 2024
Viewed by 54
Abstract
Strawberry is frequently attacked by mites, which directly affects the yield and quality of this fruit species. The WRKY Group III transcription factors (TFs) play an important role in plant tolerance to biotic sources of stress, such as pathogens and insect pests. In [...] Read more.
Strawberry is frequently attacked by mites, which directly affects the yield and quality of this fruit species. The WRKY Group III transcription factors (TFs) play an important role in plant tolerance to biotic sources of stress, such as pathogens and insect pests. In this study, six Group III WRKY TFs (FaWRKY25, FaWRKY31, FaWRKY32, FaWRKY43, FaWRKY44, and FaWRKY45) were identified in strawberry. A phylogenetic analysis showed that the six WRKY III TFs were divided into two clades and all had a conserved WRKYGQK domain and the C-X7-C-X23-H-T-C zinc finger motif. An interaction network analysis revealed that FaWRKY44 was co-expressing with FaWRKY25 and FaWRKY45. The expression patterns showed that the WRKY Group III genes responded to plant hormones and mite infestation in strawberry. To further verify the role of FaWRKY25 in plant resistance to mites, we cloned the FaWRKY25 gene and overexpressed it in transgenic plants. An in vivo subcellular localization analysis indicated that the FaWRKY25 protein was localized in the nucleus. Fewer mites were also detected on the wild-type plants than on FaWRKY25-overexpressing transgenic plants, suggesting that FaWRKY25 negatively regulates the resistance of strawberry to mites. The present study advances our understanding on a potential target that mites use to manipulate host plant defenses. Full article
(This article belongs to the Special Issue Growth, Development, and Stress Response of Horticulture Plants)
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18 pages, 9968 KiB  
Article
Active Disturbance Rejection Flight Control and Simulation of Unmanned Quad Tilt Rotor eVTOL Based on Adaptive Neural Network
by Bohai Deng, Jinfa Xu, Xingyu Yuan and Shengxin Yu
Drones 2024, 8(10), 560; https://doi.org/10.3390/drones8100560 - 8 Oct 2024
Viewed by 196
Abstract
The unmanned quad tilt-rotor eVTOL (QTRV) is a variable-configuration aircraft that combines the features of vertical takeoff and landing (VTOL), hovering, and high-speed cruising, making its control system design particularly challenging. The flight dynamics of the QTRV differ significantly between the VTOL and [...] Read more.
The unmanned quad tilt-rotor eVTOL (QTRV) is a variable-configuration aircraft that combines the features of vertical takeoff and landing (VTOL), hovering, and high-speed cruising, making its control system design particularly challenging. The flight dynamics of the QTRV differ significantly between the VTOL and cruise modes, and are further influenced by rotor tilt and external wind disturbances. Developing a unified, highly coupled nonlinear full-flight dynamics model facilitates flight control system design and simulation verification. To ensure stable tilt of the QTRV, a tilt corridor was established, along with the design of its tilt route and manipulation strategy. An adaptive neural network active disturbance rejection controller (ANN-ADRC) is proposed to ensure stable flight across all modes, reducing the control parameters and simplifying tuning while effectively estimating and compensating for unknown disturbances in real time. A hardware-in-the-loop (HIL) simulation system was designed for full-mode flight control simulation, and the results demonstrated the effectiveness of the proposed control method. Full article
(This article belongs to the Section Drone Design and Development)
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14 pages, 4550 KiB  
Article
Tunable Acoustic Tweezer System for Precise Three-Dimensional Particle Manipulation
by Jiyun Nan, Hiep Xuan Cao, Jong-Oh Park, Eunpyo Choi and Byungjeon Kang
Micromachines 2024, 15(10), 1240; https://doi.org/10.3390/mi15101240 - 8 Oct 2024
Viewed by 187
Abstract
This study introduces a tunable acoustic tweezer system designed for precise three-dimensional particle trapping and manipulation. The system utilizes a dual-liquid-layer acoustic lens, which enables the dynamic control of the focal length through the adjustable curvature of a latex membrane. This tunability is [...] Read more.
This study introduces a tunable acoustic tweezer system designed for precise three-dimensional particle trapping and manipulation. The system utilizes a dual-liquid-layer acoustic lens, which enables the dynamic control of the focal length through the adjustable curvature of a latex membrane. This tunability is essential for generating the acoustic forces necessary for effective manipulation of particles, particularly along the direction of acoustic wave propagation (z-axis). Experiments conducted with spherical particles as small as 1.5 mm in diameter demonstrated the system’s capability for stable trapping and manipulation. Performance was rigorously evaluated through both z-axis and 3D manipulation tests. In the z-axis experiments, the system achieved a manipulation range of 33.4–53.4 mm, with a root-mean-square error and standard deviation of 0.044 ± 0.045 mm, which highlights its precision. Further, the 3D manipulation experiments showed that particles could be accurately guided along complex paths, including multilayer rectangular and helical trajectories, with minimal deviation. A visual feedback-based particle navigation system significantly enhanced positional accuracy, reducing errors relative to open-loop control. These results confirm that the tunable acoustic tweezer system is a robust tool for applications requiring precise control of particles with diameter of 1.5 mm in three-dimensional environments. Considering its ability to dynamically adjust the focal point and maintain stable trapping, this system is well suited for tasks demanding high precision, such as targeted particle delivery and other applications involving advanced material manipulation. Full article
(This article belongs to the Special Issue Acoustic Transducers and Their Applications, 2nd Edition)
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16 pages, 912 KiB  
Article
Health-Related Quality of Life in Rugby Athletes: The Role of Dietary Supplements and Their Consumption
by Walter Sapuppo, Antonietta Monda, Davide Giacconi, Regina Gregori Grgič, Daniele Saccenti, Claudia Maria Mineo, Vincenzo Monda, Salvatore Allocca, Maria Casillo, Marcellino Monda, Girolamo Di Maio and Marco La Marra
Sports 2024, 12(10), 270; https://doi.org/10.3390/sports12100270 - 8 Oct 2024
Viewed by 232
Abstract
This study investigates dietary supplement use among rugby players and their general health, focusing on prevalence and underlying motivations. Involving 92 athletes, it examines the relationship between supplement usage, motivations, and health outcomes using the 36-item Short Form Health Survey and a 24-item [...] Read more.
This study investigates dietary supplement use among rugby players and their general health, focusing on prevalence and underlying motivations. Involving 92 athletes, it examines the relationship between supplement usage, motivations, and health outcomes using the 36-item Short Form Health Survey and a 24-item ad hoc questionnaire. Findings reveal a high frequency of supplement usage, motivated by desires to enhance performance, appearance, and mood. Significant differences in health-related quality of life are found between users and non-users, particularly in mental health, social functioning, and emotional stability. Motivations like performance enhancement and body shape manipulation were linked to altered health perceptions, indicating the psychosocial impacts of supplementation. This study emphasizes the need to consider the holistic effects of supplements on athlete well-being, advocating for a balanced approach prioritizing both physical and mental health. It calls for increased awareness among athletes, coaches, and sports professionals about the potential risks and benefits of supplement use and the importance of informed decision-making. Additionally, it highlights the need for further research to understand the mechanisms of supplement use and its impact on athlete health, aiming to enhance sports science and promote overall athlete well-being in competitive environments. Full article
(This article belongs to the Special Issue Exploring the Role of Acute Supplementation in Exercise Performance)
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15 pages, 3256 KiB  
Article
Mathematical Analysis of a Navier–Stokes Model with a Mittag–Leffler Kernel
by Victor Tebogo Monyayi, Emile Franc Doungmo Goufo and Ignace Tchangou Toudjeu
AppliedMath 2024, 4(4), 1230-1244; https://doi.org/10.3390/appliedmath4040066 - 8 Oct 2024
Viewed by 409
Abstract
In this paper, we establish the existence and uniqueness results of the fractional Navier–Stokes (N-S) evolution equation using the Banach fixed-point theorem, where the fractional order β is in the form of the Atangana–Baleanu–Caputo fractional order. The iterative method combined with the Laplace [...] Read more.
In this paper, we establish the existence and uniqueness results of the fractional Navier–Stokes (N-S) evolution equation using the Banach fixed-point theorem, where the fractional order β is in the form of the Atangana–Baleanu–Caputo fractional order. The iterative method combined with the Laplace transform and Sumudu transform is employed to find the exact and approximate solutions of the fractional Navier–Stokes equation of a one-dimensional problem of unsteady flow of a viscous fluid in a tube. In the domains of science and engineering, these methods work well for solving a wide range of linear and nonlinear fractional partial differential equations and provide numerical solutions in terms of power series, with terms that are simple to compute and that quickly converge to the exact solution. After obtaining the solutions using these methods, we use Mathematica software Version 13.0.1.0 to present them graphically. We create two- and three-dimensional plots of the obtained solutions at various values of β and manipulate other variables to visualize and model relationships between the variables. We observe that as the fractional order β becomes closer to the integer order 1, the solutions approach the exact solution. Lastly, we plot a 2D graph of the first-, second-, third-, and fourth-term approximations of the series solution and observe from the graph that as the number of iterations increases, the approximate solutions become close to the series solution of the fourth-term approximation. Full article
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21 pages, 9019 KiB  
Article
Aberration Modulation Correlation Method for Dim and Small Space Target Detection
by Changchun Jiang, Junwei Li, Shengjie Liu and Hao Xian
Remote Sens. 2024, 16(19), 3729; https://doi.org/10.3390/rs16193729 - 8 Oct 2024
Viewed by 245
Abstract
The significance of detecting faint and diminutive space targets cannot be overstated, as it underpins the preservation of Earth’s orbital environment’s safety and long-term sustainability. Founded by the different response characteristics between targets and backgrounds to aberrations, this paper proposes a novel aberration [...] Read more.
The significance of detecting faint and diminutive space targets cannot be overstated, as it underpins the preservation of Earth’s orbital environment’s safety and long-term sustainability. Founded by the different response characteristics between targets and backgrounds to aberrations, this paper proposes a novel aberration modulation correlation method (AMCM) for dim and small space target detection. By meticulously manipulating the light path using a wavefront corrector via a modulation signal, the target brightness will fluctuate periodically, while the background brightness remains essentially constant. Benefited by the strong correlation between targets’ characteristic changes and the modulation signal, dim and small targets can be effectively detected. Rigorous simulations and practical experiments have validated the remarkable efficacy of AMCM. Compared to conventional algorithms, AMCM boasts a substantial enhancement in the signal-to-noise ratio (SNR) detection limit from 5 to approximately 2, with an area under the precision–recall curve of 0.9396, underscoring its ability to accurately identify targets while minimizing false positives. In essence, AMCM offers an effective method for detecting dim and small space targets and is also conveniently integrated into other passive target detection systems. Full article
(This article belongs to the Special Issue Recent Advances in Infrared Target Detection)
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18 pages, 2191 KiB  
Article
Meta-Analysis Reveals Behavioral Plasticity, Not Agonistic Behavior, Facilitates Invasion of Brown Anoles (Anolis sagrei) and Replacement of Green Anoles (Anolis carolinensis)
by Maya A. Jackson and Sonny S. Bleicher
Diversity 2024, 16(10), 620; https://doi.org/10.3390/d16100620 - 8 Oct 2024
Viewed by 363
Abstract
In a meta-analysis, we examined the behavioral portfolio of invasive brown anoles (Anolis sagrei) and native green anoles (Anolis carolinensis) in urban and non-urban environments. We hypothesized that invasive anoles would display more agonistic and bold signals (head bobbing, [...] Read more.
In a meta-analysis, we examined the behavioral portfolio of invasive brown anoles (Anolis sagrei) and native green anoles (Anolis carolinensis) in urban and non-urban environments. We hypothesized that invasive anoles would display more agonistic and bold signals (head bobbing, dewlap extensions, and pushups) than their native-range counterparts and their native competitors. We found that in urban settings, anoles of both species signaled more with dewlap extensions than with head bobs. Brown anoles displayed significantly more in non-urban habitats and their native range compared to urban habitats and invasive ranges. The outcome of our analysis suggests that brown anoles have plastic behavioral portfolios, whereas green anoles have relatively balanced preferences for head bobbing, irrespective of the habitat in which the populations were collected. We attribute the success of the brown anole invasion to the flexible strategy in the face of higher mate competition, higher predation risks, and less resource competition in both urban and invasive ranges. Lastly, we observed publication biases. More studies were conducted with urban and invasive brown anoles and specifically in manipulative mesocosm experimental settings—transplanting populations from native field settings. We show this altered the display rates across all studied signals. Full article
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14 pages, 1903 KiB  
Review
Recent Advancements in High-Frequency Ultrasound Applications from Imaging to Microbeam Stimulation
by Min Gon Kim, Changhan Yoon and Hae Gyun Lim
Sensors 2024, 24(19), 6471; https://doi.org/10.3390/s24196471 - 8 Oct 2024
Viewed by 374
Abstract
Ultrasound is a versatile and well-established technique using sound waves with frequencies higher than the upper limit of human hearing. Typically, therapeutic and diagnosis ultrasound operate in the frequency range of 500 kHz to 15 MHz with greater depth of penetration into the [...] Read more.
Ultrasound is a versatile and well-established technique using sound waves with frequencies higher than the upper limit of human hearing. Typically, therapeutic and diagnosis ultrasound operate in the frequency range of 500 kHz to 15 MHz with greater depth of penetration into the body. However, to achieve improved spatial resolution, high-frequency ultrasound (>15 MHz) was recently introduced and has shown promise in various fields such as high-resolution imaging for the morphological features of the eye and skin as well as small animal imaging for drug and gene therapy. In addition, high-frequency ultrasound microbeam stimulation has been demonstrated to manipulate single cells or microparticles for the elucidation of physical and functional characteristics of cells with minimal effect on normal cell physiology and activity. Furthermore, integrating machine learning with high-frequency ultrasound enhances diagnostics, including cell classification, cell deformability estimation, and the diagnosis of diabetes and dysnatremia using convolutional neural networks (CNNs). In this paper, current efforts in the use of high-frequency ultrasound from imaging to stimulation as well as the integration of deep learning are reviewed, and potential biomedical and cellular applications are discussed. Full article
(This article belongs to the Special Issue Ultrasonic Imaging and Sensors II)
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27 pages, 3881 KiB  
Article
Neuroergonomic Attention Assessment in Safety-Critical Tasks: EEG Indices and Subjective Metrics Validation in a Novel Task-Embedded Reaction Time Paradigm
by Bojana Bjegojević, Miloš Pušica, Gabriele Gianini, Ivan Gligorijević, Sam Cromie and Maria Chiara Leva
Brain Sci. 2024, 14(10), 1009; https://doi.org/10.3390/brainsci14101009 - 7 Oct 2024
Viewed by 628
Abstract
Background/Objectives: This study addresses the gap in methodological guidelines for neuroergonomic attention assessment in safety-critical tasks, focusing on validating EEG indices, including the engagement index (EI) and beta/alpha ratio, alongside subjective ratings. Methods: A novel task-embedded reaction time paradigm was developed to evaluate [...] Read more.
Background/Objectives: This study addresses the gap in methodological guidelines for neuroergonomic attention assessment in safety-critical tasks, focusing on validating EEG indices, including the engagement index (EI) and beta/alpha ratio, alongside subjective ratings. Methods: A novel task-embedded reaction time paradigm was developed to evaluate the sensitivity of these metrics to dynamic attentional demands in a more naturalistic multitasking context. By manipulating attention levels through varying secondary tasks in the NASA MATB-II task while maintaining a consistent primary reaction-time task, this study successfully demonstrated the effectiveness of the paradigm. Results: Results indicate that both the beta/alpha ratio and EI are sensitive to changes in attentional demands, with beta/alpha being more responsive to dynamic variations in attention, and EI reflecting more the overall effort required to sustain performance, especially in conditions where maintaining attention is challenging. Conclusions: The potential for predicting the attention lapses through integration of performance metrics, EEG measures, and subjective assessments was demonstrated, providing a more nuanced understanding of dynamic fluctuations of attention in multitasking scenarios, mimicking those in real-world safety-critical tasks. These findings provide a foundation for advancing methods to monitor attention fluctuations accurately and mitigate risks in critical scenarios, such as train-driving or automated vehicle operation, where maintaining a high attention level is crucial. Full article
(This article belongs to the Special Issue Computational Intelligence and Brain Plasticity)
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22 pages, 2437 KiB  
Article
Genomic Characterization and Establishment of a Genetic Manipulation System for Trichoderma sp. (Harzianum Clade) LZ117
by Jie Yang, Cristopher Reyes Loaiciga, Hou-Ru Yue, Ya-Jing Hou, Jun Li, Cheng-Xi Li, Jing Li, Yue Zou, Shuai Zhao, Feng-Li Zhang and Xin-Qing Zhao
J. Fungi 2024, 10(10), 697; https://doi.org/10.3390/jof10100697 - 7 Oct 2024
Viewed by 306
Abstract
Abstract: Trichoderma species have been reported as masters in producing cellulolytic enzymes for the biodegradation of lignocellulolytic biomass and biocontrol agents against plant pathogens and pests. In our previous study, a novel Trichoderma strain LZ117, which shows potent capability in cellulase production, [...] Read more.
Abstract: Trichoderma species have been reported as masters in producing cellulolytic enzymes for the biodegradation of lignocellulolytic biomass and biocontrol agents against plant pathogens and pests. In our previous study, a novel Trichoderma strain LZ117, which shows potent capability in cellulase production, was isolated. Herein, we conducted multilocus phylogenetic analyses based on DNA barcodes and performed time-scaled phylogenomic analyses using the whole genome sequences of the strain, annotated by integrating transcriptome data. Our results suggest that this strain represents a new species closely related to T. atrobrunneum (Harzianum clade). Genes encoding carbohydrate-active enzymes (CAZymes), transporters, and secondary metabolites were annotated and predicted secretome in Trichoderma sp. LZ117 was also presented. Furthermore, genetic manipulation of this strain was successfully achieved using PEG-mediated protoplast transformation. A putative transporter gene encoding maltose permease (Mal1) was overexpressed, which proved that this transporter does not affect cellulase production. Moreover, overexpressing the native Cre1 homolog in LZ117 demonstrated a more pronounced impact of glucose-caused carbon catabolite repression (CCR), suggesting the importance of Cre1-mediated CCR in cellulase production of Trichoderma sp. LZ117. The results of this study will benefit further exploration of the strain LZ117 and related species for their applications in bioproduction. Full article
27 pages, 1291 KiB  
Review
A Review of Epithelial Ion Transporters and Their Roles in Equine Infectious Colitis
by Lillian M. B. Haywood and Breanna J. Sheahan
Vet. Sci. 2024, 11(10), 480; https://doi.org/10.3390/vetsci11100480 - 7 Oct 2024
Viewed by 491
Abstract
Equine colitis is a devastating disease with a high mortality rate. Infectious pathogens associated with colitis in the adult horse include Clostridioides difficile, Clostridium perfringens, Salmonella spp., Neorickettsia risticii/findlaynesis, and equine coronavirus. Antimicrobial-associated colitis can be associated with [...] Read more.
Equine colitis is a devastating disease with a high mortality rate. Infectious pathogens associated with colitis in the adult horse include Clostridioides difficile, Clostridium perfringens, Salmonella spp., Neorickettsia risticii/findlaynesis, and equine coronavirus. Antimicrobial-associated colitis can be associated with the presence of infectious pathogens. Colitis can also be due to non-infectious causes, including non-steroidal anti-inflammatory drug administration, sand ingestion, and infiltrative bowel disease. Current treatments focus on symptomatic treatment (restoring fluid and electrolyte balance, preventing laminitis and sepsis). Intestinal epithelial ion channels are key regulators of electrolyte (especially sodium and chloride) and water movement into the lumen. Dysfunctional ion channels play a key role in the development of diarrhea. Infectious pathogens, including Salmonella spp. and C. difficile, have been shown to regulate ion channels in a variety of ways. In other species, there has been an increased interest in ion channel manipulation as an anti-diarrheal treatment. While targeting ion channels also represents a promising way to manage diarrhea associated with equine colitis, ion channels have not been well studied in the equine colon. This review provides an overview of what is known about colonic ion channels and their known or putative role in specific types of equine colitis due to various pathogens. Full article
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25 pages, 47764 KiB  
Article
Efficient Regeneration of Transgenic Rice from Embryogenic Callus via Agrobacterium-Mediated Transformation: A Case Study Using GFP and Apple MdFT1 Genes
by Van Giap Do, Seonae Kim, Nay Myo Win, Soon-Il Kwon, Hunjoong Kweon, Sangjin Yang, Juhyeon Park, Gyungran Do and Youngsuk Lee
Plants 2024, 13(19), 2803; https://doi.org/10.3390/plants13192803 - 6 Oct 2024
Viewed by 422
Abstract
Genetic transformation is a critical tool for gene manipulation and functional analyses in plants, enabling the exploration of key phenotypes and agronomic traits at the genetic level. While dicotyledonous plants offer various tissues for in vitro culture and transformation, monocotyledonous plants, such as [...] Read more.
Genetic transformation is a critical tool for gene manipulation and functional analyses in plants, enabling the exploration of key phenotypes and agronomic traits at the genetic level. While dicotyledonous plants offer various tissues for in vitro culture and transformation, monocotyledonous plants, such as rice, have limited options. This study presents an efficient method for genetically transforming rice (Oryza sativa L.) using seed-derived embryogenic calli as explants. Two target genes were utilized to assess regeneration efficiency: green fluorescent protein (eGFP) and the apple FLOWERING LOCUS T (FT)-like gene (MdFT1). Antisense MdFT1 was cloned into a vector controlled by the rice α-amylase 3D (Ramy3D) promoter, while eGFP was fused to Cas9 under the Ubi promoter. These vectors were introduced separately into rice embryogenic calli from two Korean cultivars using Agrobacterium-mediated transformation. Transgenic seedlings were successfully regenerated via hygromycin selection using an in vitro cultivation system. PCR confirmed stable transgene integration in the transgenic calli and their progeny. Fluorescence microscopy revealed eGFP expression, and antisense MdFT1-expressing lines exhibited notable phenotypic changes, including variations in plant height and grain quality. High transformation efficiency and regeneration frequency were achieved for both tested cultivars. This study demonstrated the effective use of seed-derived embryogenic calli for rice transformation, offering a promising approach for developing transgenic plants in monocot species. Full article
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