Search Results (159,453)

Human Parvovirus B19 (hB19V) is a widespread virus, causing erythema infectiosum in children and several clinical manifestations from acute to persistent infections in adults. In early 2024, ECDC reported an increased human Parvovirus B19 circulation in 14 European countries. A hB19V outbreak was also reported in Tuscany, Italy, prompting a detailed investigation of its genetic characteristics. In this context, through strict monitoring of circulating strains via next-generation sequencing (NGS), we carried out a phylogenetic analysis based on the whole of hB19V genomes. Phylogenetic clustering assigned all isolates to the G1a genotype, although with some mutations in NS1, VP1, and VP2, compared to the reference strains. Further characterization of these variants is necessary to fully assess their potential implications for public health. This study provides valuable insights into the spread of Parvovirus B19 and underlines the importance of continuous genomic surveillance to monitor and respond to possible hB19V epidemics that could impact public health. Full article

Ensuring animal health and well-being requires animal-based measures for early and direct intervention at the point of care. Insight into the pathophysiology of Swine Inflammation and Necrosis Syndrome (SINS) has led to a non-invasive, clinical scoring system that can be used in daily practice. This provides information on the cause of observed lesions for direct intervention in affected herds. The aim of the current study was to re-evaluate the practicality of the SINS scoring system under field conditions. In addition, this is the first study to provide insight into the prevalence of SINS on Dutch farms. This study involved the scoring of 5958 piglets from 20 visits on 13 farms randomly selected by a veterinary practice in the Netherlands. The results showed that up to 64.1% of the piglets had visible inflammatory alterations in different body parts within the first seven days of life. Sow rectal temperature, signs of coprostasis and water intake during pregnancy were significantly (p ≤ 0.05) correlated with the prevalence of SINS in piglets. In conclusion, the SINS scoring system is an easy-to-use, non-invasive diagnostic tool that summarises animal-based observations at the point of care, providing a valuable communication tool between farmers, nutritionists and veterinarians in their efforts to improve animal health and welfare. Full article

Background: Despite the growing popularity of artificial intelligence (AI)-based systems such as ChatGPT, there is still little evidence of their effectiveness in audiology, particularly in pediatric audiology. The present study aimed to verify the performance of ChatGPT in this field, as assessed by both students and professionals, and to compare its Polish and English versions. Methods: ChatGPT was presented with 20 questions, which were posed twice, first in Polish and then in English. A group of 20 students and 16 professionals in the field of audiology and otolaryngology rated the answers on a Likert scale of 1 to 5 in terms of correctness, relevance, completeness, and linguistic accuracy. Both groups were also asked to assess the usefulness of ChatGPT as a source of information for patients, in educational settings for students, and in professional work. Results: Both students and professionals generally rated ChatGPT’s responses to be satisfactory. For most of the questions, ChatGPT’s responses were rated somewhat higher by the students than the professionals, although statistically significant differences were only evident for completeness and linguistic accuracy. Those who rated ChatGPT’s responses more highly also rated its usefulness more highly. Conclusions: ChatGPT can possibly be used for quick information retrieval, especially by non-experts, but it lacks the depth and reliability required by professionals. The different ratings given by students and professionals, and its language dependency, indicate it works best as a supplementary tool, not as a replacement for verifiable sources, particularly in a healthcare setting. Full article

Introduction: This case report examines the impact of a novel combined neuromuscular electrical stimulation (Comb-NMES) regimen on muscle glucose signaling, fiber type distribution, and metabolic function in a patient with acute spinal cord injury (SCI) and lower motor neuron lesions (LMNLs). Case Report: A 32-year-old male with complete T9 SCI underwent a ten-session Comb-NMES intervention targeting the quadriceps. Muscle biopsies and blood samples were analyzed pre- and post-intervention to evaluate changes in muscle fiber types, key metabolic proteins, fasting insulin, glucose, and lipid profiles. Results: The intervention led to a 74.7% and 28.2% reduction in fasting insulin and glucose, respectively. Muscle analysis showed significant increases in CaMK II, Hexokinase II, and IRS-1, indicating improved glucose metabolism. Conclusion: Comb-NMES training markedly improved metabolic control and muscle glucose metabolism in a patient with acute SCI and LMNLs. Enhanced insulin sensitivity and glucose utilization were evidenced by upregulated metabolic proteins, which suggests that Comb-NMES is a promising intervention for improving muscle and metabolic health in SCI patients with LMNLs. Further studies are needed to confirm these benefits and explore the long-term effects. Full article

This study investigates the relationship between lameness, milk composition, and rumination behaviour in dairy cows by leveraging sensor-based data for automated monitoring. Lameness was found to significantly impact both rumination and milk production. Lameness was assessed in 24 multiparous Holstein dairy cows throughout early lactation (up to 100 days postpartum), utilising a 1-to-5 scale. Lameness was found to significantly impact both rumination and milk production. On the day of diagnosis, rumination time decreased by 26.64% compared to the pre-diagnosis period (p < 0.01) and by 26.06% compared to healthy cows, indicating the potential of rumination as an early health indicator. The milk yield on the day of diagnosis was 28.10% lower compared to pre-diagnosis levels (p < 0.01) and 40.46% lower than healthy cows (p < 0.05). These findings suggest that lameness manifests prior to clinical signs, affecting productivity and welfare. Milk composition was also influenced, with lame cows exhibiting altered fat (+0.68%, p < 0.05) and lactose (−2.15%, p < 0.05) content compared to healthy cows. Positive correlations were identified between rumination time and milk yield (r = 0.491, p < 0.001), while negative correlations were observed between milk yield and milk fat, protein, and the fat-to-protein ratio (p < 0.001). Additionally, lameness was associated with elevated somatic cell counts in the milk, although sample size limitations necessitate further validation. This study highlights the critical role of rumination and milk performance metrics in identifying subclinical lameness, emphasising the utility of automated systems in advancing dairy cow welfare and productivity. The findings underscore the importance of early detection and management strategies to mitigate the economic and welfare impacts of lameness in dairy farming. Full article

Although worry is a normal cognitive process experienced by adolescents, for a significant number, it can reach intense and uncontrollable levels. If left untreated, these worries can lead to significant mental health problems that are maintained into adulthood. Adolescents with neurodevelopmental disorders (NDDs) may be more prone to cognitive biases (that precede worry) and therefore highly vulnerable to worry. Limited research has examined worry in adolescents with NDDs; however, most studies have focused on measuring anxiety. The present research administered an instrument specifically developed to measure worry to 404 10 to 16-year-old mainstream school-aged adolescents, 204 (123 males, 81 females) of whom had a formally diagnosed NDD. A measure of depressive symptoms was also administered. Confirmatory factor analysis revealed a satisfactory fitting model for worry. Multivariate analysis of variance revealed no interaction effects or main effect for worry according to NDD/non-NDD status. There were, however, main effects for sex, with females scoring significantly higher than males on worry about academic success and the future; worry about peer relationships; combined worry score; and depressive symptoms. The findings of this study offer psychologists and educators a brief validated measure of worry that is suited to mainstream school adolescents with or without NDDs. The wider implications of the findings in the context of education and intervention for students with NDDs are discussed. Full article

Background: There is insufficient evidence regarding the independent risk of childhood/adolescent obesity for morbidity and mortality in adulthood. The objective of the present study was to evaluate the association of weight categories during adolescence with high-risk diseases determined by the Charlson Comorbidity Index in young adulthood. We also analyzed the association of weight categories with cumulative mortality at the age of 30. Methods: A retrospective cohort study, based on the central computerized database of a major health service organization, was conducted. The study population consisted of 80,853 adolescents. The study period was from 1 January 2007 to 31 December 2022 and was divided into the exposure period from 1 January 2007 to 31 December 2011 (ages 17–19) and the follow-up period from 1 January 2007 to 31 December 2022 (from the date of the defining BMI measurement up to the age of 30 years). Results: The five diseases with the highest cumulative incidence were chronic pulmonary disease (8.2%), mild liver disease (3.7%), cerebrovascular disease (2.8%), diabetes without end-organ damage (2.0%), and peptic disease (1.6%). When adjusted for socio-demographic variables and adult BMI, the relative risks with 95% confidence intervals for the increase in the Charlson Comorbidity Index were 1.11 (1.05–1.17), 1.17 (1.11–1.24), and 1.22 (1.09–1.35) for the “overweight”, “obesity”, and “class 2 obesity” categories, respectively, while the mortality for these categories were 1.60 (1.11–2.27), 1.71 (1.12–2.57), and 3.18 (1.48–6.35), respectively. Conclusions: Adolescent obesity is an independent risk factor for high-risk diseases and mortality in young adulthood. Interventions aimed at reducing the rate of adolescent overweight and obesity should be implemented as early as possible. Full article

This study aims to examine context-specific post-migration stress factors and their differential impacts on the mental health of Syrian refugee women resettled in Houston, Texas, and urban communities in Jordan. A cross-sectional survey investigated sociodemographic and health-related conditions, psychological distress and coping (Perceived Stress Scale [PSS]), mental health-related symptomatology (Self-Report Questionnaire [SRQ]), conflict-related psychological distress (Afghan Symptom Checklist [ASC]), and post-migration stress (Refugee Post-Migration Stress Scale [RPMS]). Linear regression models examined factors associated with post-migration stress and mental health outcomes. A total of 127 Syrian refugee women participated in the study. Participants were in their mid-30s (mean age = 34.79 ± 11.2 years), married (66.9%), and reported low levels of education (44.8% below high school), low employment (27.2%), and elevated financial strain (91% below the poverty line). Jordan-based refugees exhibited higher scores on mental distress measures compared to their Houston-based counterparts; specifically more elevated psychological distress (p < 0.001), symptomatology (p < 0.001), and conflict-related distress (p < 0.001). Syrian refugee women in Houston reported higher social strain, while those in Jordan experienced greater financial hardship and barriers to accessing healthcare services. Mental distress among Syrian refugee women is influenced by specific post-migration stressors that vary by resettlement location. Targeted interventions are necessary to improve mental health outcomes in this population. Full article

Beneficial soil microorganisms, particularly plant probiotic bacteria (PPB), play a pivotal role in promoting plant growth, development, and overall health through root colonization. PPB-based biofertilizers offer a sustainable and eco-friendly alternative to conventional agricultural inputs. This study evaluates the plant probiotic potential of three native bacterial strains Rhizobium sp. ACO-34A, Sinorhizobium mexicanum ITTG R7^T, and Sinorhizobium chiapasense ITTG S70^T to enhance the growth, quality, and sugar content of Agave tequilana. A comprehensive genomic and functional analysis was conducted for each strain to assess their plant probiotic traits. Additionally, a greenhouse inoculation assay was performed on six-month-old agave seedlings at the “piña” stage to evaluate the effects of these strains on plant growth and sugar content. Comparative genomic analysis revealed that these rhizobial strains harbor genes associated with key plant probiotic traits, reinforcing their role in enhancing plant development. The results demonstrated significant effects (p < 0.05) on growth and sugar content in inoculated plants. ACO-34A increased plant height by 35.4%, fresh weight by 41.5%, and inulin content by 57.3%, while ITTG-R7^T showed improvements of 26.4%, 35.2%, and 38.2%, respectively, compared to the control, and ITTG S70^T also exhibited enhancements, although to a lesser extent, with increases of 23.5% in plant height, 28.9% in fresh weight, and 31.2% in inulin content. These findings highlight the biofertilizer potential of these native rhizobial strains, particularly Rhizobium sp. ACO-34A, positioning them as promising candidates for the sustainable cultivation of A. tequilana. Full article

Garlic (Allium sativum L.) has been extensively studied for its therapeutic and culinary applications, owing to its sulfur-containing bioactive compounds, including allicin and its derivatives. This study identified garlic varieties with high allicin content from different regions of Korea. It explores the synthesis of allithiamine, a lipid-soluble derivative of thiamine with enhanced bioavailability, by combining garlic with various grains. High-performance liquid chromatography (HPLC) analysis revealed significant regional variations in the allicin content, with Jeju garlic exhibiting the highest levels (1.04 mg/g). Among the grains tested, Avena sativa showed the most effective interaction with garlic, yielding the highest allithiamine levels (14.93 mg/g). These findings underscore the importance of grain matrix properties in optimizing the synthesis of allithiamine. This study provides valuable insights into the development of functional foods that leverage the bioactive compounds in garlic to enhance metabolic health and thiamine bioavailability. Full article

Objectives: Informed by implementation science, this exploratory study examined the capabilities, opportunities, and motivations of Australian mental health clinicians (N = 38) associated with the implementation of exposure and response prevention (ERP) for youth (i.e., children and adolescents) with obsessive-compulsive disorder (OCD). It also explored how the capabilities and motivations of mental health clinicians untrained in ERP for youth (i.e., typical clinicians; n = 25) differed from clinicians experienced in the use of ERP for youth (i.e., experienced clinicians; n = 13). Methods: Questionnaires were administered to the entire sample alongside observational role-plays, which assessed observed adherence and competence delivering ERP against published best-practice standards among available participants. Results: In the whole sample, the reported time dedicated to implementing ERP was associated with a range of factors relating to capabilities, opportunities, and motivations to implement ERP. Experienced clinicians had greater knowledge, adherence, competence, and self-reported confidence using ERP and fewer negative beliefs about ERP relative to typical clinicians. They also intended to dedicate greater time to implementing within-session ERP for youth and reported greater within- and between-session time spent doing so compared to typical clinicians. The time intended to dedicate to implementing between-sessions ERP did not significantly differ between the clinician groups. Conclusions: In summary, compared to typical clinicians, experienced clinicians appeared to possess greater levels of capabilities and motivations to implement ERP for youth with OCD. Future research examining barriers and facilitators of ERP implementation in community settings and identifying effective strategies to improve it would benefit service provision and, ultimately, outcomes for youth. Full article

Background: Chronic Obstructive Pulmonary Disease (COPD) is a disease with high morbidity and mortality globally. Exacerbations of COPD are major contributors to disease progression and a decline in health-related quality of life (HRQoL). High-flow nasal cannula (HFNC) oxygen therapy is an innovative therapy that provides humidified and heated blended air and oxygen through a nasal cannula. There is some preliminary evidence supporting the effectiveness of HFNC in managing COPD exacerbations, but there are limited data on its effectiveness when used at home for patients with stable, severe COPD. The aim of the present study is to test the hypothesis that home HFNC can decrease the COPD exacerbations rate and hospital admissions and improve HRQoL measures in severe COPD patients with frequent COPD exacerbations. Methods: In a prospective proof-of-concept interventional multicenter study, 40 GOLD stage III and IV COPD patients with a high disease burden (≥2 exacerbations treated with antibiotics and/or corticosteroids) and ≥1 hospital admission in the last year were included. Patients were given instructions on the usage of HFNC by a ventilation practitioner during a group session. The flow rate was 25–30 L/min and FiO₂ was 21–35%. Outcome measures included the COPD exacerbations rate, hospital admissions, in-hospital days, Medical Research Council dyspnea (MRC) score, Clinical COPD Questionnaire (CCQ) score, Hospital Anxiety Depression Scale (HADS) scores and capillary pCO₂. Repeated analysis of variance (ANOVA) was used to analyze the data. Significant effects identified in the ANOVA were further examined using Student’s t-tests. Results: After 1 year, 27 patients could be evaluated. The COPD exacerbations rate decreased by 1.40 (mean difference ± SD: 1.40 ± 2.09; p = 0.002), hospital admissions decreased by 0.96 admissions per year (0.96 ± 1.37; p = 0.001), and in-hospital days decreased by 7.22 days (7.22 ± 9.26; p = 0.001). Capillary pCO₂ decreased by 0.02 kPa (0.02 ± 0.52; p = 0.85). The CCQ score decreased by 0.06 (0.06 ± 0.96; p = 0.76). The MRC dyspnea score decreased by 0.04 (0.04 ± 0.80; p = 0.81). The HADS anxiety score decreased by 0.63 (0.63 ± 3.12; p = 0.31). And finally, the HADS depression score decreased by 0.32 (0.32 ± 3.48; p = 0.64). There was a significant difference between the normocapnic (capillary pCO₂ < 6.0 kPa) group and the hypercapnic group in terms of change in the CCQ score (−0.24 ± 0.55 and 0.49 ± 1.32 decrease, respectively, p = 0.05) and the HADS depression score (−0.76 ± 1.86 and 2.20 ± 4.75 decrease, respectively, p = 0.03) after 1 year of HFNC treatment. Conclusions: One-year-long HFNC therapy significantly decreased the COPD exacerbations rate, hospital admissions, and in-hospital days in severe COPD patients with a high disease burden and frequent COPD exacerbations irrespective of them having hypercapnia and with the HRQoL measures only improving in the hypercapnic group. This may imply that severe COPD patients with a high disease burden and frequent COPD exacerbations, irrespective being hypercapnic, are candidates for treatment with home HFNC oxygen therapy. Full article

Interventions taking place on the land are culturally well aligned for Native peoples, as they are often developed by the community and incorporate traditional knowledge, values, and practices. However, research on the effectiveness and characteristics of such programs is lacking. This scoping review examined outdoor and land-based prevention interventions for Indigenous adolescents ages 10–25 in the United States and Canada to identify program characteristics such as origination, aims, activities, duration, evaluation methods, and outcomes. Over three-fourths (77%) of the 153 programs identified were community-derived. The programs were principally strength-based and promoted protective factors for general wellbeing. The most common delivery format was short camps. Nearly all programs (97%) included an element of culture. The activities most often seen were recreation (84%), subsistence living (65%), and Elder knowledge sharing (63%). Thirty-three studies measured outcomes and included quantitative, qualitative, and mixed method study designs. Studies found positive impacts on participants’ self-esteem and mental health; connections to culture, cultural pride, and identity; and connections to community including peers and Elders. The literature on outdoor and land-based prevention interventions for Indigenous youth is growing rapidly. Understanding program components is a first step to identifying the elements critical to effective programs for Indigenous youth. Full article

The present study focuses on the preservation of historic timber constructions, crucial cultural heritage assets that demand effective structural health monitoring (SHM) to ensure safety and integrity. SHM aims to detect and evaluate potential structural deviations that may compromise performance, requiring both detailed geometric data acquisition and 3D modeling. For this purpose, contactless tools such as photogrammetry, laser scanning, and other topographic methods are employed to gather point cloud data. This research utilizes a terrestrial laser scanner (TLS) to generate 3D models of the historic timber tower of St. Michaeler church in Vienna. A novel automated modeling method is compared with two manual modeling approaches. The first is a traditional as-designed structural model created in Dlubal RSTAB software, and the second is a manually generated as-built model created using a scan-to-BIM application in Revit. While the first model is based on 2D plan documents created from the TLS point cloud, the second and automated models use the point cloud as direct input. The findings demonstrate that this automated model significantly enhances early-stage structural assessment efficiency, providing reliable insights into structural conditions with minimal processing time. This research underscores the potential of automated 3D modeling in preliminary structural assessments of historic timber structures. Full article

Invasive mosquitoes continue to spread, increasing the threat of mosquito-borne disease. Ongoing mosquito surveillance is necessary to track the introduction and establishment of these species in new areas and implement appropriate public health and vector control measures. Contributions from citizen science initiatives have been an important component in detecting, controlling, and raising awareness of invasive mosquitoes. The open-access biodiversity platform iNaturalist is an extensive source of human observations of wildlife, including arthropod vectors, and can be a useful supplementary tool for passive vector surveillance. In this study, the utility of iNaturalist data to support invasive mosquito surveillance was assessed by examining the distribution and seasonal data on four invasive Aedes species (IAS) in Europe and neighbouring countries. Almost 16,000 iNaturalist observations of mosquitoes were examined across 62 countries; 13% were identified as IAS, with a further 2% considered probable IAS. These included 16 observations of Aedes aegypti, 1582 Aedes albopictus, 373 Aedes japonicus, and 58 Aedes koreicus. iNaturalist observations of IAS were present in most known areas of establishment, but potential new regions of spread were also identified. These results further support the use of iNaturalist data as a low-cost source of arthropod data to assist existing vector surveillance. Full article