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Search Results (769)

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Keywords = sensor packaging

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20 pages, 9707 KiB  
Review
Advancements in Superhydrophobic Paper-Based Materials: A Comprehensive Review of Modification Methods and Applications
by Yin Tang, Shouwei Ban, Zhihan Xu, Jing Sun and Zhenxin Ning
Nanomaterials 2025, 15(2), 107; https://doi.org/10.3390/nano15020107 (registering DOI) - 12 Jan 2025
Viewed by 76
Abstract
Superhydrophobic paper-based functional materials have emerged as a sustainable solution with a wide range of applications due to their unique water-repelling properties. Inspired by natural examples like the lotus leaf, these materials combine low surface energy with micro/nanostructures to create air pockets that [...] Read more.
Superhydrophobic paper-based functional materials have emerged as a sustainable solution with a wide range of applications due to their unique water-repelling properties. Inspired by natural examples like the lotus leaf, these materials combine low surface energy with micro/nanostructures to create air pockets that maintain a high contact angle. This review provides an in-depth analysis of recent advancements in the development of superhydrophobic paper-based materials, focusing on methodologies for modification, underlying mechanisms, and performance in various applications. The paper-based materials, leveraging their porous structure and flexibility, are modified to achieve superhydrophobicity, which broadens their application in oil–water separation, anti-corrosion, and self-cleaning. The review describes the use of these superhydrophobic paper-based materials in diagnostics, environmental management, energy generation, food testing, and smart packaging. It also discusses various superhydrophobic modification techniques, including surface chemical modification, coating technology, physical composite technology, laser etching, and other innovative methods. The applications and development prospects of these materials are explored, emphasizing their potential in self-cleaning materials, oil–water separation, droplet manipulation, and paper-based sensors for wearable electronics and environmental monitoring. Full article
(This article belongs to the Section Synthesis, Interfaces and Nanostructures)
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14 pages, 6078 KiB  
Data Descriptor
The EDI Multi-Modal Simultaneous Localization and Mapping Dataset (EDI-SLAM)
by Peteris Racinskis, Gustavs Krasnikovs, Janis Arents and Modris Greitans
Data 2025, 10(1), 5; https://doi.org/10.3390/data10010005 - 7 Jan 2025
Viewed by 398
Abstract
This paper accompanies the initial public release of the EDI multi-modal SLAM dataset, a collection of long tracks recorded with a portable sensor package. These include two global shutter RGB camera feeds, LiDAR scans, as well as inertial and GNSS data from an [...] Read more.
This paper accompanies the initial public release of the EDI multi-modal SLAM dataset, a collection of long tracks recorded with a portable sensor package. These include two global shutter RGB camera feeds, LiDAR scans, as well as inertial and GNSS data from an RTK-enabled IMU-GNSS positioning module—both as satellite fixes and internally fused interpolated pose estimates. The tracks are formatted as ROS1 and ROS2 bags, with separately available calibration and ground truth data. In addition to the filtered positioning module outputs, a second form of sparse ground truth pose annotation is provided using independently surveyed visual fiducial markers as a reference. This enables the meaningful evaluation of systems that directly utilize data from the positioning module into their localization estimates, and serves as an alternative when the GNSS reference is disrupted by intermittent signals or multipath scattering. In this paper, we describe the methods used to collect the dataset, its contents, and its intended use. Full article
15 pages, 4711 KiB  
Article
Biocompatible Heterogeneous Packaging and Laser-Assisted Fluid Interface Control for In Situ Sensor in Organ-on-a-Chip
by Yu-Hsuan Lin, Shing-Fung Lau, Yen-Pei Lu, Kuo-Cheng Huang, Chien-Fang Ding, Yu-Hsiang Tang and Hsin-Yi Tsai
Micromachines 2025, 16(1), 46; https://doi.org/10.3390/mi16010046 - 30 Dec 2024
Viewed by 522
Abstract
The development of bionic organ-on-a-chip technology relies heavily on advancements in in situ sensors and biochip packaging. By integrating precise biological and fluid condition sensing with microfluidics and electronic components, long-term dynamic closed-loop culture systems can be achieved. This study aims to develop [...] Read more.
The development of bionic organ-on-a-chip technology relies heavily on advancements in in situ sensors and biochip packaging. By integrating precise biological and fluid condition sensing with microfluidics and electronic components, long-term dynamic closed-loop culture systems can be achieved. This study aims to develop biocompatible heterogeneous packaging and laser surface modification techniques to enable the encapsulation of electronic components while minimizing their impact on fluid dynamics. Using a kidney-on-a-chip as a case study, a non-toxic packaging process and fluid interface control methods have been successfully developed. Experimentally, miniature pressure sensors and control circuit boards were encapsulated using parylene-C, a biocompatible material, to isolate biochemical fluids from electronic components. Ultraviolet laser processing was employed to fabricate structures on parylene-C. The results demonstrate that through precise control of processing parameters, the wettability of the material can be tuned freely within a contact angle range of 60° to 110°. Morphological observations and MTT assays confirmed that the material and the processing methods do not induce cytotoxicity. This technology will facilitate the packaging of various miniature electronic components and biochips in the future. Furthermore, laser processing enables rapid and precise control of interface conditions across different regions within the chip, demonstrating a high potential for customized mass production of biochips. The proposed innovations provide a solution for in situ sensing in organ-on-a-chip systems and advanced biochip packaging. We believe that the development of this technology is a critical step toward realizing the concept of “organ twin”. Full article
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57 pages, 5777 KiB  
Review
Implantable Passive Sensors for Biomedical Applications
by Panagiotis Kassanos and Emmanouel Hourdakis
Sensors 2025, 25(1), 133; https://doi.org/10.3390/s25010133 - 28 Dec 2024
Viewed by 526
Abstract
In recent years, implantable sensors have been extensively researched since they allow localized sensing at an area of interest (e.g., within the vicinity of a surgical site or other implant). They allow unobtrusive and potentially continuous sensing, enabling greater specificity, early warning capabilities, [...] Read more.
In recent years, implantable sensors have been extensively researched since they allow localized sensing at an area of interest (e.g., within the vicinity of a surgical site or other implant). They allow unobtrusive and potentially continuous sensing, enabling greater specificity, early warning capabilities, and thus timely clinical intervention. Wireless remote interrogation of the implanted sensor is typically achieved using radio frequency (RF), inductive coupling or ultrasound through an external device. Two categories of implantable sensors are available, namely active and passive. Active sensors offer greater capabilities, such as on-node signal and data processing, multiplexing and multimodal sensing, while also allowing lower detection limits, the possibility to encode patient sensitive information and bidirectional communication. However, they require an energy source to operate. Battery implantation, and maintenance, remains a very important constraint in many implantable applications even though energy can be provided wirelessly through the external device, in some cases. On the other hand, passive sensors offer the possibility of detection without the need for a local energy source or active electronics. They also offer significant advantages in the areas of system complexity, cost and size. In this review, implantable passive sensor technologies will be discussed along with their communication and readout schemes. Materials, detection strategies and clinical applications of passive sensors will be described. Advantages over active sensor technologies will be highlighted, as well as critical aspects related to packaging and biocompatibility. Full article
(This article belongs to the Special Issue Feature Review Papers in Physical Sensors)
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14 pages, 7150 KiB  
Article
The Effect of Metal Shielding Layer on Electrostatic Attraction Issue in Glass–Silicon Anodic Bonding
by Wenqi Yang, Yong Ruan and Zhiqiang Song
Micromachines 2025, 16(1), 31; https://doi.org/10.3390/mi16010031 - 28 Dec 2024
Viewed by 409
Abstract
Silicon–glass anode bonding is the key technology in the process of wafer-level packaging for MEMS sensors. During the anodic bonding process, the device may experience adhesion failure due to the influence of electric field forces. A common solution is to add a metal [...] Read more.
Silicon–glass anode bonding is the key technology in the process of wafer-level packaging for MEMS sensors. During the anodic bonding process, the device may experience adhesion failure due to the influence of electric field forces. A common solution is to add a metal shielding layer between the glass substrate and the device. In order to solve the problem of device failure caused by the electrostatic attraction phenomenon, this paper designed a double-ended solidly supported cantilever beam parallel plate capacitor structure, focusing on the study of the critical size of the window opening in the metal layer for the electric field shielding effect. The metal shield consists of 400 Å of Cr and 3400 Å of Au. Based on theoretical calculations, simulation analysis, and experimental testing, it was determined that the critical size for an individual opening in the metal layer is 180 μm × 180 μm, with the movable part positioned 5 μm from the bottom, which does not lead to failure caused by stiction due to electrostatic pull-in of the detection structure. It was proven that the metal shielding layer is effective in avoiding suction problems in secondary anode bonding. Full article
(This article belongs to the Special Issue Recent Advances in Silicon-Based MEMS Sensors and Actuators)
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15 pages, 4143 KiB  
Article
Digitalized Optical Sensor Network for Intelligent Facility Monitoring
by Esther Renner, Lisa-Sophie Haerteis, Joachim Kaiser, Michael Villnow, Markus Richter, Torsten Thiel, Andreas Pohlkötter and Bernhard Schmauss
Photonics 2025, 12(1), 18; https://doi.org/10.3390/photonics12010018 - 28 Dec 2024
Viewed by 359
Abstract
Due to their inherent advantages, optical fiber sensors (OFSs) can substantially contribute to the monitoring and performance enhancement of energy infrastructure. However, optical fiber sensor systems often are standalone solutions and do not connect to the main energy infrastructure control systems. In this [...] Read more.
Due to their inherent advantages, optical fiber sensors (OFSs) can substantially contribute to the monitoring and performance enhancement of energy infrastructure. However, optical fiber sensor systems often are standalone solutions and do not connect to the main energy infrastructure control systems. In this paper, we propose a solution for the digitalization of an optical fiber sensor system realized by the Open Platform Communications Unified Architecture (OPC UA) protocol and the Internet of Things (IoT) platform Insights Hub. The optical fiber sensor system is based on bidirectional incoherent optical frequency domain reflectometry (biOFDR) and is used for the interrogation of fiber Bragg grating (FBG) arrays. To allow for an automated sensor identification and thus measurement procedure, an optical sensor identification marker based on a unique combination of fiber Bragg gratings (FBGs) is established. To demonstrate the abilities of the digitalized sensor network, a field test was performed in a power plant test facility of Siemens Energy. Temperature measurements of a packaged FBG sensor fiber were performed with a portable demonstrator, illustrating the system’s robustness and the comprehensive data processing stream from sensor value formation to the cloud. The realized network services promote sensor data quality, fusion, and modeling, expanding opportunities using digital twin technology. Full article
(This article belongs to the Special Issue Advanced Optical Fiber Sensors for Harsh Environment Applications)
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21 pages, 5252 KiB  
Article
A Multi-Layered Origami Tactile Sensory Ring for Wearable Biomechanical Monitoring
by Rajat Subhra Karmakar, Hsin-Fu Lin, Jhih-Fong Huang, Jui-I Chao, Ying-Chih Liao and Yen-Wen Lu
Biosensors 2025, 15(1), 8; https://doi.org/10.3390/bios15010008 - 27 Dec 2024
Viewed by 664
Abstract
An origami-based tactile sensory ring utilizing multilayered conductive paper substrates presents an innovative approach to wearable health applications. By harnessing paper’s flexibility and employing origami folding, the sensors integrate structural stability and self-packaging without added encapsulation layers. Knot-shaped designs create loop-based systems that [...] Read more.
An origami-based tactile sensory ring utilizing multilayered conductive paper substrates presents an innovative approach to wearable health applications. By harnessing paper’s flexibility and employing origami folding, the sensors integrate structural stability and self-packaging without added encapsulation layers. Knot-shaped designs create loop-based systems that secure conductive paper strips and protect sensing layers. Demonstrating a sensitivity of 3.8 kPa−1 at subtle pressures (0–0.05 kPa), the sensors detect both minimal stimuli and high-pressure inputs. Electrical modeling of various origami configurations identifies designs with optimized performance with a pentagon knot offering higher sensitivity to support high-sensitivity needs. Meanwhile a square knot provides greater precision and quicker recovery, balancing sensitivity and stability for real-time feedback devices. The enhanced elastic modulus from folds remains within human skin’s elasticity range, ensuring comfort. Applications include grip strength monitoring and pulse rate detection from the thumb, capturing pulse transit time (PTT), an essential cardiovascular biomarker. This design shows the potential of origami-based tactile sensors in creating versatile, cost-effective wearable health monitoring systems. Full article
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11 pages, 4314 KiB  
Article
Microwave-Assisted Extraction of Cellulose from Aloe Vera Plant Residue and Preparation of Cellulose Nanocrystal–Poly(vinyl alcohol) Hydrogels
by Eleni Triantafyllou, Andreas Karydis-Messinis, Dimitrios Moschovas, Christina Kyriakaki, Konstantinos C. Vasilopoulos, Aris E. Giannakas, Michael A. Karakassides, Apostolos Avgeropoulos, Nikolaos E. Zafeiropoulos and Constantinos E. Salmas
Molecules 2024, 29(24), 6012; https://doi.org/10.3390/molecules29246012 - 20 Dec 2024
Viewed by 460
Abstract
Biomass valorization and bio-based material development are of major research interest following the spirit of the circular economy. Aloe vera cultivation is a widespread agricultural activity oriented toward supplement production because of its well-known antioxidant and antimicrobial properties. Aloe vera juice production also [...] Read more.
Biomass valorization and bio-based material development are of major research interest following the spirit of the circular economy. Aloe vera cultivation is a widespread agricultural activity oriented toward supplement production because of its well-known antioxidant and antimicrobial properties. Aloe vera juice production also produces a large amount of biomass byproducts that are usually landfilled. On the other hand, cellulose nanocrystals are widely used in several applications, such as biomaterials, bio-compatible polymers, nanocomposites, food packaging, medicines, cosmetics, and sensors, due to their unique physical, mechanical, optical, electrical, and healing properties as well as their compatibility with biological tissues. This study introduces a novel approach combining the microwave-assisted extraction (MAE) of cellulose from this residue with the subsequent isolation of cellulose nanocrystals (CNCs). The MAE process, which exhibits a rapid heating and penetrating ability, was optimized to maximize the cellulose yield under various conditions (microwave power, solvent ratio, and time). Analysis using FTIR, XRD, SEM, and DMA indicated that isolated pure cellulose nanocrystals and a stable PVA–CNC porous hydrogel network were produced. The PVA–CNC hydrogel was synthesized to enable the formation of a semi-crystalline network that imparts the material with enhanced mechanical properties. Both final products of this study could potentially be used for various applications. Full article
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20 pages, 5927 KiB  
Article
Design of Active Suspension Controllers for 8 × 8 Armored Combat Vehicles
by Yonghwan Jeong and Seongjin Yim
Machines 2024, 12(12), 931; https://doi.org/10.3390/machines12120931 - 18 Dec 2024
Viewed by 621
Abstract
This paper presents a method to design an active suspension controller for 8 × 8 armored combat vehicles, which is called corner damping control (CDC). It is assumed that the target vehicle with 8 × 8 drive mechanisms and 8 suspensions has active [...] Read more.
This paper presents a method to design an active suspension controller for 8 × 8 armored combat vehicles, which is called corner damping control (CDC). It is assumed that the target vehicle with 8 × 8 drive mechanisms and 8 suspensions has active actuators on each suspension for vertical, roll and pitch motion control on a sprung mass. A state-space model with 22 state variables is derived from the target vehicle. With the state-space model, a linear quadratic (LQ) cost function is defined. The control objective is to reduce the vertical acceleration, pitch and roll angles of a sprung mass for ride comfort, durability and turret stabilization. To avoid full-state feedback of LQR, a static output feedback control (SOF) is selected as a control structure for CDC. The vertical velocity, roll and pitch rates of a sprung mass, and vertical velocities at each corner, are selected as a sensor output. With those sensor outputs and LQ cost function, four LQ SOF controllers are designed. To validate the effectiveness of the LQ SOF controllers, simulation is carried out on a vehicle simulation package. From the simulation results, it is shown that the proposed CDC with LQ SOF controllers with a much smaller number of sensor outputs and controller gains can reduce the vertical acceleration, pitch and roll angles of a sprung mass and, as a result, improve ride comfort, durability and turret stabilization. Full article
(This article belongs to the Special Issue Advances in Vehicle Suspension System Optimization and Control)
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11 pages, 5810 KiB  
Article
Reading Dye-Based Colorimetric Inks: Achieving Color Consistency Using Color QR Codes
by Ismael Benito-Altamirano, Laura Engel, Ferran Crugeira, Miriam Marchena, Jürgen Wöllenstein, Joan Daniel Prades and Cristian Fàbrega
Chemosensors 2024, 12(12), 260; https://doi.org/10.3390/chemosensors12120260 - 13 Dec 2024
Viewed by 555
Abstract
Color consistency when reading colorimetric sensors is a key factor for this technology. Here, we demonstrate how the usage of machine-readable patterns, like QR codes, can be used to solve the problem. We present our approach of using back-compatible color QR codes as [...] Read more.
Color consistency when reading colorimetric sensors is a key factor for this technology. Here, we demonstrate how the usage of machine-readable patterns, like QR codes, can be used to solve the problem. We present our approach of using back-compatible color QR codes as colorimetric sensors, which are common QR codes that also embed a set of hundreds of color references as well as colorimetric indicators. The method allows locating the colorimetric sensor within the captured scene and to perform automated color correction to ensure color consistency regardless of the hardware used. To demonstrate it, a CO2-sensitive colorimetric indicator was printed on top of a paper-based substrate using screen printing. This indicator was formulated for Modified Atmosphere Packaging (MAP) applications. To verify the method, the sensors were exposed to several environmental conditions (both in gas composition and light conditions). And, images were captured with an 8M pixel digital camera sensor, similar to those used in smartphones. Our results show that the sensors have a relative error of 9% when exposed with a CO2 concentration of 20%. This is a good result for low-cost disposable sensors that are not intended for permanent use. However, as soon as light conditions change (2500–6500 K), this error increases up to ϵ20 = 440% (rel. error at 20% CO2 concentration) rendering the sensors unusable. Within this work, we demonstrate that our color QR codes can reduce the relative error to ϵ20 = 14%. Furthermore, we show that the most common color correction, white balance, is not sufficient to address the color consistency issue, resulting in a relative error of ϵ20 = 90%. Full article
(This article belongs to the Special Issue Novel Gas Sensing Approaches: From Fabrication to Application)
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22 pages, 10279 KiB  
Article
Cybersecurity Challenges in UAV Systems: IEMI Attacks Targeting Inertial Measurement Units
by Issam Boukabou, Naima Kaabouch and Dulana Rupanetti
Drones 2024, 8(12), 738; https://doi.org/10.3390/drones8120738 - 8 Dec 2024
Viewed by 1126
Abstract
The rapid expansion in unmanned aerial vehicles (UAVs) across various sectors, such as surveillance, agriculture, disaster management, and infrastructure inspection, highlights the growing need for robust navigation systems. However, this growth also exposes critical vulnerabilities, particularly in UAV package delivery operations, where intentional [...] Read more.
The rapid expansion in unmanned aerial vehicles (UAVs) across various sectors, such as surveillance, agriculture, disaster management, and infrastructure inspection, highlights the growing need for robust navigation systems. However, this growth also exposes critical vulnerabilities, particularly in UAV package delivery operations, where intentional electromagnetic interference (IEMI) poses significant security and safety threats. This paper addresses IEMI attacks targeting inertial measurement units (IMUs) in UAVs, focusing on their susceptibility to medium-power electromagnetic interference. Our approach combines a comprehensive literature review and QuickField simulation with experimental validation using a commercially available 6-degree-of-freedom (DOF) IMU sensor. We propose a hardware-based electromagnetic shielding solution using mu-metal to mitigate IEMI’s impact on sensor performance. The study combines experimental testing with simulations to evaluate the shielding effectiveness under controlled conditions. The results of the measurements showed that medium-power IEMI significantly distorted IMU sensor readings, but our proposed shielding method effectively reduces the impact, improving sensor reliability. We demonstrate the mechanisms by which medium-power IEMI disrupts sensor operation, offering insights for future research directions. These findings also highlight the importance of integrating hardware-based shielding solutions to safeguard UAV systems against electromagnetic threats. Full article
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19 pages, 3613 KiB  
Article
Near-Field Passive Wireless Sensor for High-Temperature Metal Corrosion Monitoring
by Noah Strader, Brian R. Jordan, Oguzhan Bilac, Kevin M. Tennant, Daryl S. Reynolds, Edward M. Sabolsky and Ashley C. Daniszewski
Sensors 2024, 24(23), 7806; https://doi.org/10.3390/s24237806 - 6 Dec 2024
Viewed by 681
Abstract
This work focuses on the fabrication and evaluation of a passive wireless sensor for the monitoring of the temperature and corrosion of a metal material at high temperatures. An inductor–capacitor (LC) resonator sensor was fabricated through the screen printing of Ag-based inks on [...] Read more.
This work focuses on the fabrication and evaluation of a passive wireless sensor for the monitoring of the temperature and corrosion of a metal material at high temperatures. An inductor–capacitor (LC) resonator sensor was fabricated through the screen printing of Ag-based inks on dense polycrystalline Al2O3 substrates. The LC design was modeled using the ANSYS HFSS modeling package, with the LC passive wireless sensors operating at frequencies from 70 to 100 MHz. The wireless response of the LC was interrogated and received by a radio frequency signal generator and spectrum analyzer at temperatures from 50 to 800 °C in real time. The corrosion kinetics of the Cu 110 was characterized through thermogravimetric (TGA) analysis and microscopy images, and the oxide thickness growth was then correlated to the wireless sensor signal under isothermal conditions at 800 °C. The results showed that the wireless signal was consistent with the corrosion kinetics and temperature, indicating that these two characteristics can be further deconvoluted in the future. In addition, the sensor also showed a magnitude- and frequency-dependent response to crack/spallation events in the oxide corrosion layer, permitting the in situ wireless identification of these catastrophic events on the metal surface at high temperatures. Full article
(This article belongs to the Special Issue Sensors for High Temperature Monitoring)
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27 pages, 14453 KiB  
Review
Busbar Design for High-Power SiC Converters
by Zibo Chen and Alex Q. Huang
Electronics 2024, 13(23), 4758; https://doi.org/10.3390/electronics13234758 - 2 Dec 2024
Viewed by 679
Abstract
Busbars are critical components that connect high-current and high-voltage subcomponents in high-power converters. This paper reviews the latest busbar design methodologies and offers design recommendations for both laminated and PCB-based busbars. Silicon Carbide (SiC) power devices switch at much higher speeds compared to [...] Read more.
Busbars are critical components that connect high-current and high-voltage subcomponents in high-power converters. This paper reviews the latest busbar design methodologies and offers design recommendations for both laminated and PCB-based busbars. Silicon Carbide (SiC) power devices switch at much higher speeds compared to traditional silicon devices, making them more susceptible to parasitic elements within the busbar. In high-frequency SiC converters, using thicker copper offers limited improvement in high-frequency current handling due to the reduced skin depth at such frequencies. PCB busbars, however, provide several advantages, including reduced loop inductance, enhanced high-frequency current capacity, simplified assembly, and lower costs. Additionally, they enable the integration of components such as sensors, capacitors, and resistors, which can further optimize overall system performance. This paper also presents optimized busbar designs for both module-based and discrete device-based SiC high-power converters, comparing various SiC power module packages and offering design insights. Finally, this paper showcases a 75 kW three-phase inverter utilizing a PCB busbar, demonstrating its potential for achieving high power density and cost-effectiveness in discrete SiC device-based high-power converters. Full article
(This article belongs to the Special Issue Advances in Power Converter Design, Control and Applications)
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13 pages, 3295 KiB  
Article
In Vivo Quantification of Surfactin Nonribosomal Peptide Synthetase Complexes in Bacillus subtilis
by Maliheh Vahidinasab, Lisa Thewes, Bahar Abrishamchi, Lars Lilge, Susanne Reiße, Elvio Henrique Benatto Perino and Rudolf Hausmann
Microorganisms 2024, 12(11), 2381; https://doi.org/10.3390/microorganisms12112381 - 20 Nov 2024
Viewed by 796
Abstract
Surfactin, a potent biosurfactant produced by Bacillus subtilis, is synthesized using a non-ribosomal peptide synthetase (NRPS) encoded by the srfAA-AD operon. Despite its association with quorum sensing via the ComX pheromone, the dynamic behavior and in vivo quantification of the NRPS complex [...] Read more.
Surfactin, a potent biosurfactant produced by Bacillus subtilis, is synthesized using a non-ribosomal peptide synthetase (NRPS) encoded by the srfAA-AD operon. Despite its association with quorum sensing via the ComX pheromone, the dynamic behavior and in vivo quantification of the NRPS complex remain underexplored. This study established an in vivo quantification system using fluorescence labeling to monitor the availability of surfactin-forming NRPS subunits (SrfAA, SrfAB, SrfAC, and SrfAD) during bioprocesses. Four Bacillus subtilis sensor strains were constructed by fusing these subunits with the megfp gene, resulting in strains BMV25, BMV26, BMV27, and BMV28. These strains displayed growth and surfactin productivity similar to those of the parental strain, BMV9. Fluorescence signals indicated varying NRPS availability, with BMV27 showing the highest and BMV25 showing the lowest relative fluorescence units (RFUs). RFUs were converted to the relative number of NRPS molecules using open-source FPCountR package. During bioprocesses, NRPS availability peaked at the end of the exponential growth phase and declined in the stationary phase, suggesting reduced NRPS productivity under nutrient-limited conditions and potential post-translational regulation. This study provides a quantitative framework for monitoring NRPS dynamics in vivo, offering insights into optimizing surfactin production. The established sensor strains and quantification system enable the real-time monitoring of NRPS availability, aiding bioprocess optimization for industrial applications of surfactin and potentially other non-ribosomal peptides. Full article
(This article belongs to the Special Issue Advances in Microbial Surfactants: Production and Applications)
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26 pages, 1397 KiB  
Article
Inertial Measurement Unit Self-Calibration by Quantization-Aware and Memory-Parsimonious Neural Networks
by Matteo Cardoni, Danilo Pietro Pau, Kiarash Rezaei and Camilla Mura
Electronics 2024, 13(21), 4278; https://doi.org/10.3390/electronics13214278 - 31 Oct 2024
Viewed by 2317
Abstract
This paper introduces a methodology to compensate inertial Micro-Electro-Mechanical System (IMU-MEMS) time-varying calibration loss, induced by stress and aging. The approach relies on a periodic assessment of the sensor through specific stimuli, producing outputs which are compared with the response of a high-precision [...] Read more.
This paper introduces a methodology to compensate inertial Micro-Electro-Mechanical System (IMU-MEMS) time-varying calibration loss, induced by stress and aging. The approach relies on a periodic assessment of the sensor through specific stimuli, producing outputs which are compared with the response of a high-precision sensor, used as ground truth. At any re-calibration iteration, differences with respect to the ground truth are approximated by quantization-aware trained tiny neural networks, allowing calibration-loss compensations. Due to the unavailability of aging IMU-MEMS datasets, a synthetic dataset has been produced, taking into account aging effects with both linear and nonlinear calibration loss. Also, field-collected data in conditions of thermal stress have been used. A model relying on Dense and 1D Convolution layers was devised and compensated for an average of 1.97 g and a variance of 1.07 g2, with only 903 represented with 16 bit parameters. The proposed model can be executed on an intelligent signal processing inertial sensor in 126.4 ms. This work represents a step forward toward in-sensor machine learning computing through integrating the computing capabilities into the sensor package that hosts the accelerometer and gyroscope sensing elements. Full article
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