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19 pages, 5171 KiB  
Article
Optimised Design and Simulation Analysis of a Double-Row Pneumatic Injection Seeding Device
by Qi Niu, Wei Yu, Wenhao Yan, Helong Zhang, Lihong Wang, Chengsong Li and Chunlei Wang
Agriculture 2024, 14(8), 1376; https://doi.org/10.3390/agriculture14081376 - 16 Aug 2024
Viewed by 174
Abstract
Stem mustard, the main raw material for pickled mustard tuber, is widely planted in Chongqing, China, and is an important local cash crop. Under the working conditions of sticky and wet soil in the Chongqing area, conventional furrow seeding has problems such as [...] Read more.
Stem mustard, the main raw material for pickled mustard tuber, is widely planted in Chongqing, China, and is an important local cash crop. Under the working conditions of sticky and wet soil in the Chongqing area, conventional furrow seeding has problems such as soil sticking to the furrow opener, poor mulching effect, etc. In this regard, this paper proposes the use of non-contact, soil-based, pneumatic shot seeding, in which seeds are shot into the soil to a predetermined depth by a high-speed air stream. The diameter of stem mustard seeds was found to be 1.33 mm, with a spherical rate of 95.32% using physical and mechanical properties. The high-speed camera test was used to determine the air pressure at the appropriate sowing depth, and the seed entry process was simulated by EDEM 2021 software, which analysed the movement process of the seed after entering the soil, and the structure of the seeder was designed based on the resulting test data. The structural parameters of the shot seeding device were analysed by a hydrodynamic simulation using Fluent 2022 R1 software and the following results were obtained: an outlet pipe diameter DC of 2 mm, mixing zone length H of 10 mm, mixing zone inlet diameter D of 15 mm, and steady-state gas flow rate of 80 m/s. Simulation seeding verification was conducted on the final determined structural parameters of the seeding device, and the simulation results showed that the seed velocity could reach 32.3 m/s. In actual experiments, it was found that when the vertical velocity of the seeds was greater than or equal to 26.59 m/s, the seeds could be completely and stably seeded into the soil. Therefore, the designed seeding device can meet the conditions of actual seeding experiments. In conclusion, this research offers a practical guideline for the rapid and precise sowing of stem mustard. Full article
(This article belongs to the Section Agricultural Technology)
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18 pages, 2284 KiB  
Article
Effects of Establishment on Growth, Yield, and Silage Qualities of Amaranth in Typhoon-Prone Southern Kyushu, Japan
by Zixuan Zhong, Mitsuhiro Niimi, Manabu Tobisa, Sachiko Idota and Yasuyuki Ishii
Agriculture 2024, 14(8), 1364; https://doi.org/10.3390/agriculture14081364 - 15 Aug 2024
Viewed by 291
Abstract
Amaranth (Amaranthus hypochondriacus L.) is a potential forage crop with a high yield and crude protein (CP) content; however, establishment methods need to improve for the crop to be less sensitive to typhoons. Optimal establishment, cultivation, and utilization in amaranth were examined [...] Read more.
Amaranth (Amaranthus hypochondriacus L.) is a potential forage crop with a high yield and crude protein (CP) content; however, establishment methods need to improve for the crop to be less sensitive to typhoons. Optimal establishment, cultivation, and utilization in amaranth were examined in a variety of seasons and methods of establishment in 2021–2023. Four methods were examined: (1) direct seeding in rows, (2) direct seeding in spots, (3) soil seed balls, and (4) transplant pretreatment methods under a randomized blocked design (n = 3). Sowings every month from April to August were applied only in 2021, while establishments in April, May, and August with both pretreatment methods were applied in 2022 and 2023. The establishment in August successfully escaped damage from typhoons. The direct seeding of either rows or spots showed marginal success in establishment compared to stable establishment in pretreatment methods. In 2022 and 2023, the highest yield and CP content were achieved in soil seed balls plots in April and in both pretreated plots sown in August, respectively. The quality of silage fermentation showed a high pH, ranging from 4.52 to 6.39, due to the high CP content in 7.59–18.36% dry matter (DM). Sowing in April or August established with soil seed balls can avoid typhoon damage to have stable forage yields and can be processed with a favorable quality of amaranth silage in the region. Full article
(This article belongs to the Section Crop Production)
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13 pages, 1875 KiB  
Article
The Effects of Soil Acidity and Aluminium on the Root Systems and Shoot Growth of Lotus pedunculatus and Lupinus polyphyllus
by Lucy E. Bell, Jim L. Moir and Alistair D. Black
Plants 2024, 13(16), 2268; https://doi.org/10.3390/plants13162268 - 15 Aug 2024
Viewed by 218
Abstract
Lotus pedunculatus (lotus) and Lupinus polyphyllus (Russell lupin) persist in the upland grasslands of New Zealand, where soil acidity and associated aluminium (Al) toxicity impede conventional pasture legumes. This experiment investigated the response of lotus and Russell lupin to soil acidity and Al. [...] Read more.
Lotus pedunculatus (lotus) and Lupinus polyphyllus (Russell lupin) persist in the upland grasslands of New Zealand, where soil acidity and associated aluminium (Al) toxicity impede conventional pasture legumes. This experiment investigated the response of lotus and Russell lupin to soil acidity and Al. The species were sown in 20 cm tall 1.2 L pots of acidic upland soil. A mass of 4.5 or 6.7 g lime (CaCO3)/L was added to either the top or bottom or both soil horizons (0–9 cm and 9–18 cm), resulting in six treatments across six randomised blocks in a glasshouse. The soil pH was 4.4, 4.9, and 5.4; the exchangeable Al concentrations were 24, 2.5, and 1.5 mg/kg for 0, 4.5, and 6.7 g lime/L. At 16 weeks post-sowing, the plants were divided into shoots and roots at 0–9 cm and 9–18 cm. Root morphology, shoot and root dry matter (DM), shoot nitrogen (N), and nodulation were measured. The total plant DM and shoot-to-root DM ratio were higher, and the shoot %N was lower for the lotus plants than the Russell lupin plants for the various lime rates (13.2 vs. 2.9 g plant−1, 5.6 vs. 1.6, and 2.4 vs. 3.3%, p < 0.05). No response to lime in terms of total DM or total root morphology parameters was exhibited in either species (p > 0.05). Root morphology adjustments in response to acidity between soil horizons were not observed. The results indicated that lotus and Russell lupin are tolerant to high soil acidity (pH 4.4–5.4) and exchangeable Al (1.5–24 mg kg−1), highlighting their considerable adaptation to grasslands with acidic soils. Full article
(This article belongs to the Special Issue Phosphorus and pH Management in Soil–Plant Systems)
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15 pages, 1022 KiB  
Article
Long-Term Effect of Tillage Systems on Planosol Physical Properties, CO2 Emissions and Spring Barley Productivity
by Aušra Sinkevičienė, Kęstutis Romaneckas, Karolina Jackevičienė, Toma Petrikaitė, Jovita Balandaitė and Rasa Kimbirauskienė
Land 2024, 13(8), 1289; https://doi.org/10.3390/land13081289 - 15 Aug 2024
Viewed by 208
Abstract
As the population grows, more food is needed to keep the food supply chain running smoothly. For many years, intensive farming systems have been used to meet this need. Currently, due to intense climate change and other global natural problems, there is a [...] Read more.
As the population grows, more food is needed to keep the food supply chain running smoothly. For many years, intensive farming systems have been used to meet this need. Currently, due to intense climate change and other global natural problems, there is a shift towards sustainable use of natural resources and simplified methods of tillage. Soil tillage intensity influences the distribution of nutrients, and soil’s physical and mechanical properties, as well as gas flows. The impact of reduced tillage on these indices in spring barley cultivation is still insufficient and requires more analysis on a global scale. This study was carried out at Vytautas Magnus University, Agriculture Academy (Lithuania) in 2022–2023. The aim of the investigation was to determine the effect of the tillage systems on the soil temperature, moisture content, CO2 respiration and concentration in spring barley cultivation. Based on a long-term tillage experiment, five tillage systems were tested: deep and shallow moldboard ploughing, deep cultivation-chiseling, shallow cultivation-chiseling, and no tillage Shallow plowing technology has been found to better conserve soil moisture and maintain higher temperatures in most cases. During almost the entire study period, the spring barley crop with deep cultivation had lower moisture content and lower soil temperature. Shallow cultivation fields in most cases increased CO2 emissions and CO2 concentration. When applying direct sowing to the uncultivated soil (10–20 cm), the concentration of CO2 decreased from 0.01 to 0.148 percent. pcs. The results show that in direct sowing fields, most cases had a positive effect on crop density. Direct sowing fields resulted in significantly lower, from 7.9 to 26.5%, grain yields of spring barley in the years studied. Full article
(This article belongs to the Special Issue Tillage Methods on Soil Properties and Crop Growth)
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10 pages, 1535 KiB  
Article
Generation of Two-Line Restorer Line with Low Chalkiness Using Knockout of Chalk5 through CRISPR/Cas9 Editing
by Gucheng Fan, Jiefeng Jiang, Yu Long, Run Wang, Famao Liang, Haiyang Liu, Junying Xu, Xianjin Qiu and Zhixin Li
Biology 2024, 13(8), 617; https://doi.org/10.3390/biology13080617 - 15 Aug 2024
Viewed by 229
Abstract
Chalkiness is an important grain quality trait in rice. Chalk5, encoding a vacuolar H+-translocating pyrophosphatase, is a major gene affecting both the percentage of grains with chalkiness (PGWC) and chalkiness degree (DEC) in rice. Reducing its expression can decrease both [...] Read more.
Chalkiness is an important grain quality trait in rice. Chalk5, encoding a vacuolar H+-translocating pyrophosphatase, is a major gene affecting both the percentage of grains with chalkiness (PGWC) and chalkiness degree (DEC) in rice. Reducing its expression can decrease both PGEC and DEC. In this study, the first exon of Chalk5 was edited in the elite restorer line 9311 using the CRISPR/Cas9 system and two knockout mutants were obtained, one of which did not contain the exogenous Cas9 cassette. PGWC and DEC were both significantly reduced in both mutants, while the seed setting ratio (SSR) was also significantly decreased. Staggered sowing experiments showed that the chalkiness of the mutants was insensitive to temperature during the grain-filling stage, and the head milled rice rate (HMRR) could be improved even under high-temperature conditions. Finally, in the hybrid background, the mutants showed significantly reduced PGWC and DEC without changes in other agronomic traits. The results provide important germplasm and allele resources for breeding high-yield rice varieties with superior quality, especially for high-yield indica hybrid rice varieties with superior quality in high-temperature conditions. Full article
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27 pages, 4005 KiB  
Article
Options for Intensification of Cropping System in Coastal Saline Ecosystem: Inclusion of Grain Legumes in Rice-Based Cropping System
by Sukamal Sarkar, Koushik Brahmachari, Donald S. Gaydon, Anannya Dhar, Saikat Dey and Mohammed Mainuddin
Soil Syst. 2024, 8(3), 90; https://doi.org/10.3390/soilsystems8030090 - 14 Aug 2024
Viewed by 268
Abstract
The coastal saline zone of West Bengal in India is the home to millions of the world’s poorest and most vulnerable people. Due to a gradual increase in salt accumulation on soils of the coastal saline zone of West Bengal in India from [...] Read more.
The coastal saline zone of West Bengal in India is the home to millions of the world’s poorest and most vulnerable people. Due to a gradual increase in salt accumulation on soils of the coastal saline zone of West Bengal in India from winter to summer days, cultivation of the second crop in the winter season becomes possible in a limited area. To address these issues, field experiments was conducted in rainy and winter seasons of 2016–17 and 2017–18 at the farmer’s field of the coastal saline zone (CSZ) of West Bengal, India. The experiment was carried out to study the system productivity, nutrient uptake, and profitability vis-à-vis salinity dynamics of the crops in rice-pulse-based cropping systems under different land elevations (medium upland and medium lowland). The experiment was conducted in a strip-split plot design having horizontal factors namely, Factor A: Six dates of sowing of rice at an interval of one week (2nd week of June to 3rd week of July), Factor B: Two land situations (medium upland and medium lowland) and Two Cropping Systems (Rice-Lathyrus and Rice-Lentil) as vertical factor, replicated four times. The results suggest that irrespective of land situation, early sown rice (15 June to 21 June) produces higher dry matter and grain yield compared to late sown crops. This early sowing of rice also facilitated the better performance of subsequent lathyrus and lentil, by avoiding the worst situation of the salinity build-up and drought stress later in the winter. Moreover, significantly higher productions were obtained from medium-lowland situations for both the cropping systems. Sowing date has also significantly influenced macro-nutrient uptake (NPK) by rice and pulse grains. It may be concluded that early sowing of rice may be a potential option for intensification of rice-pulse-based cropping systems under CSZ of West Bengal, India. Full article
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21 pages, 7226 KiB  
Article
Analysis of Maize Planting Mode and Simulation and Optimization of Ridging and Fertilization Components in Arid Area of Northwest China
by Fei Dai, Haifu Pan, Wenqi Zhou, Han Tang, Qi Wang, Wenglong Li and Jinwu Wang
Agriculture 2024, 14(8), 1360; https://doi.org/10.3390/agriculture14081360 - 14 Aug 2024
Viewed by 264
Abstract
The arid area of Northwest China belongs to the rain-fed agricultural area of the Loess Plateau, and water resources have become one of the important factors limiting agricultural development in this area. This study employed the AquaCrop model to predict the yield advantages [...] Read more.
The arid area of Northwest China belongs to the rain-fed agricultural area of the Loess Plateau, and water resources have become one of the important factors limiting agricultural development in this area. This study employed the AquaCrop model to predict the yield advantages and environmental adaptability of maize in Dingxi City from 2016 to 2020 under two cultivation practices: ridge tillage (100% film coverage with double ridge-furrow planting) and flat planting (81.8% film coverage with wide-film planting). The numerical simulation of the tillage and fertilization process of the double-ridge seedbed was carried out by EDEM, and the key components were tested by the Box–Behnken center combination test design principle to obtain the optimal parameter combination. The results showed that ridge planting was more suitable for agricultural planting in rain-fed arid areas in Northwest China. The simulation analysis of ridging and fertilization showed that the forward speed of the combined machine was 0.50 m/s, the rotation speed of the trough wheel of the fertilizer discharger was 39 rmp, and the rotary tillage depth was 150 mm. The qualified rate of seedbed tillage was 93.6%, and the qualified rate of fertilization was 92.1%. The research shows that the whole-film double ridge-furrow sowing technology of maize is more suitable for the rain-fed agricultural area in the arid area of Northwest China. The simulation results of the ridging fertilization device are consistent with the field experiment results. The research results provide a certain technical reference for the optimization of the whole-film double ridge-furrow sowing technology. Full article
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3 pages, 204 KiB  
Correction
Correction: Gómez et al. Long-Term Effect of Maternal Antioxidant Supplementation on the Lipid Profile of the Progeny According to the Sow’s Parity Number. Antioxidants 2024, 13, 379
by Gerardo Gómez, Hernan D. Laviano, Juan García-Casco, Maria Muñoz, Fernando Gómez, Fernando Sánchez-Esquiliche, Antonio González-Bulnes, Clemente López-Bote, Cristina Óvilo and Ana I. Rey
Antioxidants 2024, 13(8), 987; https://doi.org/10.3390/antiox13080987 - 14 Aug 2024
Viewed by 108
Abstract
In the original publication [...] Full article
12 pages, 1172 KiB  
Article
Association Analysis of METTL23 Gene Polymorphisms with Reproductive Traits in Kele Pigs
by Jie Sun, Chunyuan Wang, Yan Wu, Jin Xiang and Yiyu Zhang
Genes 2024, 15(8), 1061; https://doi.org/10.3390/genes15081061 - 12 Aug 2024
Viewed by 308
Abstract
Methyltransferase-like 23 (METTL23) is a kind of RNA methyltransferase that catalyzes the methylation transfer to the N6-adenosine of RNA, serving as one of the key mediators in this process. However, the METTL23 gene has been poorly researched in pigs. In [...] Read more.
Methyltransferase-like 23 (METTL23) is a kind of RNA methyltransferase that catalyzes the methylation transfer to the N6-adenosine of RNA, serving as one of the key mediators in this process. However, the METTL23 gene has been poorly researched in pigs. In this study, we investigated the genetic effects of METTL23 single-nucleotide polymorphism(SNPs) on reproductive traits in Kele pigs. The DNA was extracted from 228 healthy multiparous Kele sows, and Sanger sequencing revealed three SNPs, g.4804958 G > T (intron 2), g.4805082 C > T (exon 2), and g.4806821 A > G (exon 3). The polymorphism information content (PIC) for each SNP was 0.264, 0.25, and 0.354, indicating moderate polymorphism (0.25 < PIC < 0.5) and providing genetic information. Linkage disequilibrium analysis showed no strong linkage disequilibrium between the three SNPs. The association analysis revealed that in the SNP g.4804958 G > T individuals with the GG genotype had a significantly higher number of piglets born alive, litter birth weight, number of weaned piglets, and weaning litter weight compared to those with the TT genotype (p < 0.05). Individuals with the GG genotype in the SNP g.4806821 A > G group had significantly higher litter birth weight and average birth weight than those with the AA genotype (p < 0.05). The H4H4 diplotype showed significant effects on the number of piglets born alive, litter birth weight, number of weaned piglets, weaning litter weight, and weaning weight (p < 0.05). Together, the METTL23 gene could be used as a candidate gene for the selection of reproductive traits in Kele pigs. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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20 pages, 6094 KiB  
Article
Effects of Acremonium terricola Culture on Lactation Performance, Immune Function, Antioxidant Capacity, and Intestinal Flora of Sows
by Zhirong Chen, Lixia Xiao, Qian Sun, Qiangqiang Chen, Weidong Hua and Jinzhi Zhang
Antioxidants 2024, 13(8), 970; https://doi.org/10.3390/antiox13080970 - 9 Aug 2024
Viewed by 319
Abstract
This study aimed to determine the effects of different doses of Acremonium terricola culture (ATC) on lactation performance, immune function, antioxidant capacity, and intestinal flora of sows. Forty-five Landrace sows (3–6 parity) were randomly assigned to the following three treatments from 85 days [...] Read more.
This study aimed to determine the effects of different doses of Acremonium terricola culture (ATC) on lactation performance, immune function, antioxidant capacity, and intestinal flora of sows. Forty-five Landrace sows (3–6 parity) were randomly assigned to the following three treatments from 85 days of gestation to 21 days after farrowing: a control diet (CON, basal diet), a low-dose Acremonium terricola culture diet (0.2% ATC, basal diet + 0.2% ATC), and a high-dose Acremonium terricola culture diet (0.4% ATC, basal diet + 0.4% ATC). Compared with the CON group, the supplementation of 0.2% ATC increased the average daily milk yield of sows by 4.98%, increased milk fat, total solids, and freezing point depression on day 1 postpartum (p < 0.05), increased serum concentration of Triiodothyronine, Thyroxin, and Estradiol on day 21 postpartum (p < 0.05). Compared with the CON group, the supplementation of 0.4% ATC increased the average daily milk yield of sows by 9.38% (p < 0.05). Furthermore, the supplementation of 0.2% ATC increased serum concentration of IgG, IgM, and IFN-γ, CD4 on day 1 postpartum (p < 0.05) and increased serum concentration of immunoglobulin A ( IgA), immunoglobulin G (IgG), immunoglobulin M ( IgM), complement 3 (C3), cluster of differentiation 4 (CD4), cluster of differentiation 8 (CD8), interferon-γ (IFN-γ) on day 21 postpartum (p < 0.05), while the supplementation of 0.4% ATC reduced serum concentration of IL-2 on day 21 postpartum (p < 0.05). Moreover, the supplementation of 0.4% ATC significantly increased serum concentration of catalase (CAT) (p < 0.05). Additionally, the supplementation of ATC affected the relative abundance of the intestinal flora at different taxonomic levels in sows and increased the abundance of beneficial bacteria such as in the norank_f__Eubacterium_coprostanoligenes group, Eubacterium_coprostanoligenes group, and Lachnospiraceae_XPB1014 group of sows, while reducing the abundance of harmful bacteria such as Phascolarctobacterium and Clostridium_sensu_stricto_1. These data revealed that the supplementation of ATC during late gestation and lactation can improve lactation performance, immune function, antioxidant capacity, and the gut microbiota. Compared with supplementation of 0.4% ATC, 0.2% ATC enhances the levels of thyroid-related hormones, specific antibodies, and cytokines in serum, promotes the diversity of beneficial gut microbiota, beneficial bacteria in the intestine, reduces the population of harmful bacteria, and thereby bolsters the immunity of sows. Hence, 0.2% ATC is deemed a more optimal concentration. Full article
(This article belongs to the Special Issue Oxidative Stress in Livestock and Poultry—2nd Edition)
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9 pages, 226 KiB  
Article
The Impact of Maize Legume Intercropping on Energy Indices and GHG Emissions as a Result of Climate Change
by Kęstutis Romaneckas, Austėja Švereikaitė, Rasa Kimbirauskienė, Aušra Sinkevičienė, Aida Adamavičienė and Algirdas Jasinskas
Agriculture 2024, 14(8), 1303; https://doi.org/10.3390/agriculture14081303 - 7 Aug 2024
Viewed by 412
Abstract
Multicropping can solve energy use and GHG balance problems, but the emergence, development, and productivity of such mixed crops are at risk due to the uneven distribution of precipitation. For this reason, investigations were performed at the Experimental Station of Vytautas Magnus University, [...] Read more.
Multicropping can solve energy use and GHG balance problems, but the emergence, development, and productivity of such mixed crops are at risk due to the uneven distribution of precipitation. For this reason, investigations were performed at the Experimental Station of Vytautas Magnus University, Lithuania. Single maize crops were compared with Crimson/red clover, Persian clover, and alfalfa intercropped maize. The objective of this study was to evaluate the main energy indices and GHG balance of legume intercropped maize cultivated in humid and arid vegetative conditions. The results showed that, under arid conditions, the quantity of intercrop biomass was about four times lower than that under humid conditions. Humid conditions were less suitable for maize and resulted in about 3–5 t ha−1 less dried biomass from intercrops and about 6 t ha−1 less biomass in single crops than in arid conditions. Due to the higher yield of maize biomass in the arid season, better energy indicators of crops were obtained in arid than humid conditions. The difference between net energy was about 122–123 MJ ha−1 in all treatments, except for the maize crop with intercropped alfalfa, where the difference was 62 MJ ha−1. All tested technologies were environmentally friendly; the CO2 equivalent varied between treatments from 804 to 884 kg ha−1. The uneven distribution of precipitation during the vegetative season provides insight into the improvement of intercropping technologies. Sowing intercrops at the same time as maize could improve their germination but increase the problem of weed spread. Full article
18 pages, 3757 KiB  
Article
Sustainability of Organic Fertilizers Use in Dryland Mediterranean Agriculture
by Carlos Ortiz, María Rosa Yagüe, Alcira Sunilda Valdez, María Gabriela Molina and Àngela Dolores Bosch-Serra
Agriculture 2024, 14(8), 1301; https://doi.org/10.3390/agriculture14081301 - 7 Aug 2024
Viewed by 450
Abstract
Organic fertilization is a key issue in European Union (EU) regulations, particularly in the context of promoting a circular nutrient economy, maintaining soil quality, and sequestering carbon to face climate change. In a rainfed system in Northeastern Spain, an experiment was set up [...] Read more.
Organic fertilization is a key issue in European Union (EU) regulations, particularly in the context of promoting a circular nutrient economy, maintaining soil quality, and sequestering carbon to face climate change. In a rainfed system in Northeastern Spain, an experiment was set up (split-plot design). It included five pre-sowing N fertilization treatments: control, mineral, pig slurry, and composted sewage sludge (two rates). The average N rates were 0, 30, 141, 176, and 351 kg N ha−1, respectively. They were combined with mineral N topdressings (0, 50, and 100 kg N ha−1). Three crops were grown: barley (nine years), wheat (three years), and rapeseed (one year). In the driest years (c. 350 mm rainfall), the yields averaged 2.5, 2.0, and 1.9 Mg ha−1, respectively. The maximum yields were for barley (6.5 Mg ha−1) and wheat (5.5 Mg ha−1). The avoidance of a significant increase in soil residual NO3-N, plus the control of soil build up of available P, micronutrients, and Cd, defines the fertilization strategies. (i) With a previous spring drought season, no fertilization is needed in the following year, if devoted to winter cereals. (ii) In rainier seasons, pig slurry or composted sewage sludge (lowest rate) applied at sowing is sufficient; however, 50 kg of mineral-N ha−1 at the topdressing can be applied. The study found that pig slurry favors K, Mg, Cu, and Zn availability, while composted sewage sludge enhances Fe availability. Although it is possible to reduce N inputs from organic fertilizers, organic C build-up will be constrained. Full article
(This article belongs to the Section Agricultural Soils)
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8 pages, 435 KiB  
Brief Report
Prevalence and Characterization of Salmonella during Pork Sausage Manufacturing
by Lauren R. Wottlin, Roger B. Harvey, Keri N. Norman, Robert E. Droleskey, Kathleen Andrews, Steve J. Jackson, Robin C. Anderson and Toni L. Poole
Microorganisms 2024, 12(8), 1599; https://doi.org/10.3390/microorganisms12081599 - 6 Aug 2024
Viewed by 402
Abstract
Pork carcasses and meat may harbor Salmonella and may contaminate other products during harvest and fabrication. Sources of contamination include manure on hides, environmental contamination, ingredients from external sources, and lymph nodes. Swine lymph nodes are often incorporated into ground meat, as their [...] Read more.
Pork carcasses and meat may harbor Salmonella and may contaminate other products during harvest and fabrication. Sources of contamination include manure on hides, environmental contamination, ingredients from external sources, and lymph nodes. Swine lymph nodes are often incorporated into ground meat, as their anatomical location makes removal labor prohibitive. A sausage processing plant in the midwestern United States was sampled monthly (except for December) from May 2021 to April 2022 to enumerate Salmonella and Enterobacteriaceae (EB) throughout the sausage manufacturing process to determine high-risk stages and efficiency of existing in-plant interventions. Salmonella serotypes and antimicrobial susceptibility were evaluated on a subset of isolates recovered at the end phases of sausage production. In each collection, samples were taken from the carcasses of eight sows through 11 stages of sausage manufacturing. A total of 830 samples were cultured. Thirty-four Salmonella were isolated from the final three production stages; of these, there were eleven serotypes. Three isolates displayed resistance to ampicillin, whereas the remainder of the isolates were pan-susceptible to the antimicrobials tested. Salmonella and EB were significantly reduced (p < 0.001) by acid washes at different stages of production, and the results point to the beneficial effects of interventions to lessen Salmonella concentrations in retail products. Full article
(This article belongs to the Special Issue Overview of Foodborne Pathogens and Antimicrobial Resistance)
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16 pages, 3107 KiB  
Article
Nitrogen Fertilization Alleviates Barley (Hordeum vulgare L.) Waterlogging
by Jianbo Chen, Chenchen Zhao, Matthew Tom Harrison and Meixue Zhou
Agronomy 2024, 14(8), 1712; https://doi.org/10.3390/agronomy14081712 - 4 Aug 2024
Viewed by 414
Abstract
Waterlogging increasingly challenges crop production, affecting 10% of global arable land, necessitating the development of pragmatic strategies for mitigating the downside risk of yield penalty. Here, we conducted experiments under controlled (tank) and field conditions to evaluate the efficacy of nitrogenous fertiliser in [...] Read more.
Waterlogging increasingly challenges crop production, affecting 10% of global arable land, necessitating the development of pragmatic strategies for mitigating the downside risk of yield penalty. Here, we conducted experiments under controlled (tank) and field conditions to evaluate the efficacy of nitrogenous fertiliser in alleviating waterlogging stress. Without intervention, we found that waterlogging reduced grain yields, spike numbers and shoot biomass, but had a de minimus impact on grain number per spike and increased grain weight. Soil fertiliser mitigated waterlogging damage, enhancing yields via increased spike numbers, with crop recovery post-waterlogging catalysed via improved tiller numbers, plant height and canopy greenness. Foliar nitrogen spray has little impact on crop recovery, possibly due to stomatal closure, while modest urea application during and after waterlogging yielded similar results to greater N application at the end of waterlogging. Waterlogging-tolerant genotypes (P-17 and P-52) showed superior growth and recovery during and after waterlogging compared to the waterlogging-sensitive genotypes (Planet and P-79). A comparison of fertiliser timing revealed that field fertilizer treatment two (F2: 90 kg·ha−1 at 28 DWL, 45 kg·ha−1 at sowing and 45 kg·ha−1 at 30 DR) yielded the highest and fertilizer treatment three (F3: 45 kg·ha−1 at sowing and 45 kg·ha−1 at 30 DR) recovered the lowest yield and spike number, while fertilizer treatment one (F1: 45 kg·ha−1 at 28 DWL, 45 kg·ha−1 at 0 DR, 45 kg·ha−1 at sowing and 45 kg·ha−1 at 30 DR) and four (F4: 90 kg·ha−1 at 0 DR, 45 kg·ha−1 at sowing and 45 kg·ha−1 at 30 DR) had the highest shoot biomass in the field. Treatment five (T5: 180 kg·ha−1 at 0 DR, 30 kg·ha−1 at sowing and 90 kg·ha−1 at 30 DR) presented the most favourable results in the tank. Our results provide rigorous evidence that long periods of waterlogging caused significant yield penalty, mainly due to decreased spike numbers. We contend that increasing fertiliser rates during waterlogging up to 90 kg·ha−1 can provoke crop growth and mitigate waterlogging-induced grain yield losses, and is more beneficial than applying nitrogen post-waterlogging. Full article
(This article belongs to the Section Crop Breeding and Genetics)
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20 pages, 1752 KiB  
Article
Mechanized No-Tillage Planting with Maize Straw Mulching Improves Potato Yield and Water Use Efficiency in Arid Regions of Northwest China
by Hui Li, Pengxia Liu, Wei Sun, Hua Zhang, Xiaolong Liu, Peiwen Li and Fengwei Zhang
Agronomy 2024, 14(8), 1711; https://doi.org/10.3390/agronomy14081711 - 3 Aug 2024
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Abstract
To explore the yield-increasing mechanism of mechanized potato planting with corn straw mulching, a two-year (2021 and 2022) field experiment was conducted to study the effects of mechanized no-tillage with straw mulching on potato yield and water use efficiency. This experiment included mechanized [...] Read more.
To explore the yield-increasing mechanism of mechanized potato planting with corn straw mulching, a two-year (2021 and 2022) field experiment was conducted to study the effects of mechanized no-tillage with straw mulching on potato yield and water use efficiency. This experiment included mechanized no-tillage potato planting with corn straw mulch covering (JG), plastic film mulching (HM), and open flat planting (CK). The results showed that mechanical no-tillage with straw mulching significantly affected soil water content in the 0–100 cm soil layer, yield, and water use efficiency (p < 0.05). There was no significant difference in yield between JG and HM, but it was significantly higher than that of CK. The yield of JG was 3.09~12.27% higher than that of CK. The yield increase was mainly achieved by increasing the potato weight per plant (0.697~0.862 **) and the average single potato weight (0.048~0.631). The tuber weight per plant was positively correlated with the plant height at the seedling stage (0.03~0.92 **) and positively correlated with the dry weight of stems and leaves at the tuber expansion stage and starch accumulation stage (0.74 **~0.95 **). It was negatively correlated with the number of branches at the tuber formation stage (−0.33~−0.88 **). Compared with CK, JG could significantly improve the water use efficiency of potatoes and reduce water consumption during the whole growth period of crops. In 2021, JG was 6.5% higher than CK, and HM was 6.88% lower than CK. In 2022, JG and HM increased water use efficiency by 26.17% and 14.50% compared with CK. When HM is applied in heavy soil areas, soil compaction can easily occur, which affects seedling emergence and reduces yield. At the same time, JG has strong adaptability to soil types and good yield stability. It can be seen that JG is a green and efficient mechanized potato cultivation technology suitable for dry farming areas. Full article
(This article belongs to the Section Innovative Cropping Systems)
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