Search Results (996)

There is a growing need for precise and rapid detection methods in fields such as biomedical diagnostics, environmental monitoring, and chemical analysis. Surface plasmon resonance (SPR) sensors have been used for the detection and quantification of a wide range of analytes, including biomolecules, chemicals, and gases, in real-time. Despite the promising capabilities of SPR sensors, there remains a gap in creating a balance between having a large enough area to capture a significant number of analytes for detection and being small enough to ensure high sensitivity. This research aims to explore the design of a D-shaped SPR-based optical fiber sensor, focusing on the use of copper, gold, and silver thin films at optimized width and thickness of 10 µm and 45 nm, respectively, to improve the sensor’s performance. Employing a computational approach, this study examines the influence of the optimized width and refractive indices of metallic films on the sensor’s characteristics. The 10 µm width of the metallic thin film has been found to produce an optimal balance between the sensitivity and the dynamic range of the sensor. Leveraging on the ratio of the real and imaginary parts of the dielectric constant of the thin film metal provides insight into the optical properties and sensitivity at certain wavelengths. Within an analyte refractive index range of 1.37–1.42 and a wavelength range of 650–1200 nm, results indicate that silver outperforms gold and copper at the optimized width with a wavelength sensitivity, and detection accuracy of 12,300 nmRIU⁻¹, and 3.075, respectively. By optimizing the width of the metal thin film at 10 µm, a highly sensitive D-SPR is designed, allowing for enhanced sensor detection capabilities for a wide range of bioanalytes. Full article

Bone homeostasis relies on the delicate balance between osteoblast-mediated bone formation and osteoclast-mediated bone resorption. The casein kinase 2 interacting protein-1 (CKIP-1), a specific CK2α subunit-interacting protein, has been documented as one of the crucial negative regulators of bone formation. CKIP-1 siRNA therapy has constraints that limit its use in clinical applications. Therefore, it is necessary to explore effective targeting strategies for CKIP-1. In this study, we observed an upregulation of CKIP-1 protein expression in the microgravity environment, while its ubiquitination levels decreased. We further investigated the interaction between CKIP-1 and VHL and found that VHL enhanced CKIP-1 degradation through the ubiquitylation–proteasome system (UPS). Additionally, we discovered a small molecule ligand, named C77, through DNA-encoded library (DEL) screening, which binds to CKIP-1 both in vivo and in vitro, as confirmed by Surface Plasmon Resonance (SPR) and the Cellular Thermal shift assay (CETSA), respectively. Our findings demonstrated the potential of VHL and C77 as guiding factors in the development of CKIP-1-based Proteolysis-Targeting Chimeras (PROTACs), which could be future therapeutic interventions in disuse osteoporosis. Full article

2-Furaldehyde (2-FAL) is one of the main by-products of the degradation of hemicellulose, which is the solid material of the oil–paper insulating system of oil-filled transformers. For this reason, it has been suggested as a marker of the degradation of the insulating system; sensing devices for 2-FAL analysis in a wide concentration range are of high interest in these systems. An optical sensor system is proposed; this consists of a chemical chip, able to capture 2-FAL from the insulating oil, coupled with a surface plasmon resonance (SPR) probe, both realized on multimode plastic optical fibers (POFs). The SPR platform exploits gold nanofilm or, alternatively, a double layer of gold and silicon oxide to modulate the sensor sensitivity. The capturing chip is always based on the same molecularly imprinted polymer (MIP) as a receptor specific for 2-FAL. The system with the SPR probe based on a gold nanolayer had a higher sensitivity and a lower detection limit of fractions of μg L⁻¹. Instead, the SPR probe, based on a double layer (gold and silicon oxide), has a lower sensitivity with a worse detection limit, and it is suitable for the detection of 2-FAL at concentrations of 0.01–1 mg L⁻¹. Full article

Receptor–ligand interactions at cell interfaces initiate signaling cascades essential for cellular communication and effector functions. Specifically, T cell receptor (TCR) interactions with pathogen-derived peptides presented by the major histocompatibility complex (pMHC) molecules on antigen-presenting cells are crucial for T cell activation. The binding duration, or dwell time, of TCR–pMHC interactions correlates with downstream signaling efficacy, with strong agonists exhibiting longer lifetimes compared to weak agonists. Traditional surface plasmon resonance (SPR) methods quantify 3D affinity but lack cellular context and fail to account for factors like membrane fluctuations. In the recent years, single-molecule Förster resonance energy transfer (smFRET) has been applied to measure 2D binding kinetics of TCR–pMHC interactions in a cellular context. Here, we introduce a rigorous mathematical model based on survival analysis to determine exponentially distributed receptor–ligand interaction lifetimes, verified through simulated data. Additionally, we developed a comprehensive analysis pipeline to extract interaction lifetimes from raw microscopy images, demonstrating the model’s accuracy and robustness across multiple TCR–pMHC pairs. Our new software suite automates data processing to enhance throughput and reduce bias. This methodology provides a refined tool for investigating T cell activation mechanisms, offering insights into immune response modulation. Full article

Bacteria are capable of remarkable adaptations to their environment, including undesirable bacterial resistance to antibacterial agents. One of the most serious cases is an infection caused by multidrug-resistant Staphylococcus aureus, which has unfortunately also spread outside hospitals. Therefore, the development of new effective antibacterial agents is extremely important to solve the increasing problem of bacterial resistance. The bacteriolytic enzyme autolysin E (AtlE) is a promising new drug target as it plays a key role in the degradation of peptidoglycan in the bacterial cell wall. Consequently, disruption of function can have an immense impact on bacterial growth and survival. An in silico and in vitro evaluation of iminosugar derivatives as potent inhibitors of S. aureus (AtlE) was performed. Three promising hit compounds (1, 3 and 8) were identified as AtlE binders in the micromolar range as measured by surface plasmon resonance. The most potent compound among the SPR response curve hits was 1, with a K_D of 19 μM. The K_D value for compound 8 was 88 μM, while compound 3 had a K_D value of 410 μM. Full article

Molecularly imprinted membranes (MIMs) have been a focal research interest since 1990, representing a breakthrough in the integration of target molecules into membrane structures for cutting-edge sensing applications. This paper traces the developmental history of MIMs, elucidating the diverse methodologies employed in their preparation and characterization on two-dimensional solid-supported substrates. We then explore the principles and diverse applications of MIMs, particularly in the context of emerging technologies encompassing electrochemistry, surface-enhanced Raman scattering (SERS), surface plasmon resonance (SPR), and the quartz crystal microbalance (QCM). Furthermore, we shed light on the unique features of ion-sensitive field-effect transistor (ISFET) biosensors that rely on MIMs, with the notable advancements and challenges of point-of-care biochemical sensors highlighted. By providing a comprehensive overview of the latest innovations and future trajectories, this paper aims to inspire further exploration and progress in the field of MIM-driven sensing technologies. Full article

Background: Paclitaxel is a widely used cancer treatment drug that has a significant inhibitory effect on cervical cancer cells (HeLa cells). This study aims to investigate the effects of paclitaxel on HeLa cells and evaluate the application of terahertz (THz) spectroscopy and surface plasmon resonance (SPR) biosensors in this process. Methods: We utilized an SPR biosensor in conjunction with THz spectroscopy to measure the terahertz absorbance spectra of HeLa cells exposed to various concentrations of paclitaxel. The minimum number of cells used for detection was 15.25 × 10⁵. At the same time, cell proliferation levels were assessed through proliferation assays and compared with the terahertz spectroscopy data. Results: The experimental results indicated that with the increasing concentration of paclitaxel, the terahertz absorbance spectra of HeLa cells exhibited a blue shift, and cell proliferation was significantly inhibited. The results of the proliferation assays were consistent with the terahertz spectroscopy data, validating the effectiveness of this method. Conclusion: This study demonstrates that the combination of THz spectroscopy and SPR biosensors is a promising technology that can provide a simple, rapid, and low-cost method for studying chemistry–biology relationships, especially in the field of drug evaluation. Full article

In order to achieve a high-precision synchronous detection of two different refractive index (RI) analytes, a D-type surface plasmon resonance (SPR) photonic crystal fiber (PCF) RI sensor based on two channels is designed in this paper. The sensor uses a D-shaped planar region of the PCF and a large circular air hole below the core as the sensing channels. Surface plasmon resonance is induced by applying a coating of gold film on the surface. The full-vector finite-element method (FEM) is used to optimize the structural parameters of the optical fiber, and the sensing characteristics are studied, including wavelength sensitivity, RI resolution, full width at half maximum (FWHM), figure of merit (FOM), and signal-to-noise ratio (SNR). The results show that the channel 1 (Ch 1) can achieve RI detection of 1.36–1.39 in the wavelength range of 1500–2600 nm, and the channel 2 (Ch 2) can achieve RI detection of 1.46–1.57 in the wavelength range of 2100–3000 nm. The two sensing channels can detect independently or simultaneously measure two analytes with different RIs. The maximum wavelength sensitivity of the sensor can reach 30,000 nm/RIU in Channel 1 and 9900 nm/RIU in Channel 2. The RI resolutions of the two channels are 3.54 × 10⁻⁶ RIU and 10.88 × 10⁻⁶ RIU, respectively. Therefore, the sensor realizes dual-channel high- and low-RI synchronous detection in the ultra-long wavelength band from near-infrared to mid-infrared and achieves an ultra-wide RI detection range and ultra-high wavelength sensitivity. The sensor has a wide application prospect in the fields of chemical detection, biomedical sensing, and water environment monitoring. Full article

Graphene-based surface plasmon resonance (SPR) biosensors have emerged as a promising technology for the highly sensitive and accurate detection of biomolecules. This study presents a comprehensive theoretical analysis of graphene-based SPR biosensors, focusing on configurations with single and bimetallic metallic layers. In this study, we investigated the impact of various metallic substrates, including gold and silver, and the number of graphene layers on key performance metrics: sensitivity of detection, detection accuracy, and quality factor. Our findings reveal that configurations with graphene first supported on gold exhibit superior performance, with sensitivity of detection enhancements up to 30% for ten graphene layers. In contrast, silver-supported configurations, while demonstrating high sensitivity, face challenges in maintaining detection accuracy. Additionally, reducing the thickness of metallic layers by 30% optimizes light coupling and enhances sensor performance. These insights highlight the significant potential of graphene-based SPR biosensors in achieving high sensitivity of detection and reliability, paving the way for their application in diverse biosensing technologies. Our findings pretend to motivate future research focusing on optimizing metallic layer thickness, improving the stability of silver-supported configurations, and experimentally validating the theoretical findings to further advance the development of high-performance SPR biosensors. Full article

In this study, we systematically investigate theoretically and experimentally the plasmonic effect and roles of big and small gold nanoparticles (Au NPs) within a mixed solution. The polydisperse solution was initially prepared by mixing small (10, 30 nm) Au NPs with larger ones (50, 80 nm), followed by measuring the extinction using ultraviolet–visible (UV-vis) spectroscopy. The experimental results clearly showed that the extinction of the mixed solution is predominantly influenced by the presence of the larger NPs, even though their quantity is small. Subsequently, we conducted simulations to explore the plasmonic properties of Au NPs of different sizes as well as their mixings and to validate the experimental results. To explain the deviation of the extinction spectra between experimental observations and simulations, we elaborated a simulation model involving the mixture of spherical Au NPs with ellipsoidal NPs, thus showing agreement between the simulation and the experiment. By performing simulations of plasmonic near-field of NPs, our investigation revealed that the maximal electric field intensity does not occur precisely at the plasmonic resonant wavelength but rather at a nearby redder wavelength. The optimal size of the Au NP dispersed in water for achieving the highest field enhancement was found to be 60 nm, with an excitation wavelength of 553.7 nm. These interesting findings not only enrich our understanding of plasmonic NPs’ optical behavior but also guide researchers for potential applications in various domains. Full article

The frequent occurrence of viral infections poses a serious threat to human life. Identifying effective antiviral components is urgent. In China, pearls have been important traditional medicinal ingredients since ancient times, exhibiting various therapeutic properties, including detoxification properties. In this study, a peptide, KKCH, which acts against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was derived from Pinctada fucata pearls. Molecular docking showed that it bound to the same pocket of the SARS-CoV-2 S protein and cell surface target angiotensin-converting enzyme II (ACE2). The function of KKCH was analyzed through surface plasmon resonance (SPR), Enzyme-Linked Immunosorbent Assays, immunofluorescence, and simulation methods using the SARS-CoV-2 pseudovirus and live virus. The results showed that KKCH had a good affinity for ACE2 (KD = 6.24 × 10⁻⁷ M) and could inhibit the binding of the S1 protein to ACE2 via competitive binding. As a natural peptide, KKCH inhibited the binding of the SARS-CoV-2 S1 protein to the surface of human BEAS-2B and HEK293T cells. Moreover, viral experiments confirmed the antiviral activity of KKCH against both the SARS-CoV-2 spike pseudovirus and SARS-CoV-2 live virus, with half-maximal inhibitory concentration (IC₅₀) values of 398.1 μM and 462.4 μM, respectively. This study provides new insights and potential avenues for the prevention and treatment of SARS-CoV-2 infections. Full article

Cortisol is a clinically validated stress biomarker that takes part in many physiological and psychological functions related to the body’s response to stress factors. In particular, it has emerged as a pivotal tool for understanding stress levels and overall well-being. Usually, in clinics, cortisol levels are monitored in blood or urine, but significant changes are also registered in sweat and saliva. In this work, a surface plasmon resonance probe based on a D-shaped plastic optical fiber was functionalized with a glucocorticoid receptor exploited as a highly efficient bioreceptor specific to cortisol. The developed plastic optical fiber biosensor was tested for cortisol detection in buffer and artificial saliva. The biosensor response showed very good selectivity towards other hormones and a detection limit of about 59 fM and 96 fM in phosphate saline buffer and artificial saliva, respectively. The obtained detection limit, with a rapid detection time (about 5 min) and a low-cost sensor system, paved the way for determining the cortisol concentration in saliva samples without any extraction process or sample pretreatment via a point-of-care test. Full article

This work explores the transformative role of graphene in enhancing the performance of surface plasmon resonance (SPR)-based biosensors. The motivation for this review stems from the growing interest in the unique properties of graphene, such as high surface area, excellent electrical conductivity, and versatile functionalization capabilities, which offer significant potential to improve the sensitivity, specificity, and stability of SPR biosensors. This review systematically analyzes studies published between 2010 and 2023, covering key metrics of biosensor performance. The findings reveal that the integration of graphene consistently enhances sensitivity. Specificity, although less frequently reported numerically, showed promising results, with high specificity achieved at sub-nanomolar concentrations. Stability enhancements are also significant, attributed to the protective properties of graphene and improved biomolecule adsorption. Future research should focus on mechanistic insights, optimization of integration techniques, practical application testing, scalable fabrication methods, and comprehensive comparative studies. Our findings provide a foundation for future research, aiming to further optimize and harness the unique physical properties of graphene to meet the demands of sensitive, specific, stable, and rapid biosensing in various practical applications. Full article

In this study, a surface plasmon resonance (SPR) sensor based on modified plastic optical fibers (POFs) was combined with a specific molecularly imprinted polymer (MIP), used as a synthetic receptor, for glyphosate (GLY) determination in aqueous solutions. Since GLY is a non-selective herbicide associated with severe environmental and health problems, detecting glyphosate in environmental and biological samples remains challenging. The selective interaction between the MIP layer and GLY is monitored by exploiting the SPR phenomenon at the POF’s gold surface. Experimental results show that in about ten minutes and by dropping microliter volume samples, the presented optical–chemical sensor can quantify up to three orders of magnitude of GLY concentrations, from nanomolar to micromolar, due to a thin MIP layer over the SPR surface. The developed optical–chemical sensor presents a detection limit of about 1 nM and can be used for onsite GLY measurements. Moreover, the experimental analysis demonstrated the high selectivity of the proposed POF-based chemical sensor. Full article

Silver-based grating structures offer means for implementing low-cost, efficient grating couplers for use in surface plasmon resonance (SPR) sensors. One-dimensional grating structures with a fixed periodicity are confined to operate effectively within a single planar orientation. However, two-dimensional grating structures as well as grating structures with variable periodicity allow for the plasmon excitation angle to be seamlessly adjusted. This study demonstrates silver-based grating designs that allow for the plasmon excitation angle to be adjusted via rotation or beam position. The flexible angle adjustment opens up the possibility of developing SPR sensor designs with an expanded dynamic range and increased flexibility in sensing applications. The results demonstrate that efficient coupling into two diffraction orders is possible, which ultimately leads to an excitation angle range from 16° to 40° by rotating a single structure. The findings suggest a promising direction for the development of versatile and adaptable SPR sensing platforms with enhanced performance characteristics. Full article