Extended Data Fig. 1: DON inhibits cellular proliferation in a panel of PDAC lines.

Cell growth of human and murine pancreatic cancer cells (a) HY19636, (b) PaTu-8988T, (c)HY15549, (d)PANC1, (e)PaTu-8902 and (f)MiaPaCa2 exposed to DON for 72 hours at various concentrations ([DON, µM]: 500, 250, 100, 75, 50, 25, 10, and 5). Data plotted as confluency (geometric mean and error, n = 3). Significance was determined by two-way ANOVA using Dunnett’s multiple comparisons test. All the p values are included in the data source. (g) HY19636 cells were treated with DON (25 µM) for 7 days at various concentrations of glutamine (20 mM, n = 5; 10 mM, n = 6; 4 mM, n = 6; 2 mM, n = 6; 0.5 mM, n = 3). Representation of one representative experiment. Every condition was normalized to the control of each respective glutamine concentration. Data is displayed as mean ± s.e.m. The box plots extend from the 25th to 75th percentiles. The whiskers represent the smallest to largest values. The line in the middle of the box is plotted at the median. Significance was determined by ordinary one-way ANOVA using Tukey’s multiple comparisons test. P value: ****: <0.0001. All the p values are included in the data source.

Source data