Review Articles

Natural and engineered Cas12 and TnpB variants offer diverse applications in genome editing and diagnostics.

Epigenome editing has versatile applications in biomedical research and disease treatment.

Chu and colleagues discuss recent developments in de novo protein design.

Notin, Rollins and colleagues discuss advances in computational protein design with a focus on redesign of existing proteins.

Drug discovery is being transformed by advances in computational protein structure prediction and protein design.

Challenges and opportunities for engineering and studying the soil microbiome are discussed.

Pfeiffer and Stafforst review and discuss the state-of-the-art tools for precision editing of RNA bases.

mRNA therapeutics face more challenges than mRNA vaccines, but solutions are on the horizon.

Spatial transcriptomics workflows, metrics and limitations are reviewed and discussed.

Proteomics is being transformed by deep learning methods that predict peptide fragmentation spectra.

An overview of apps for outbreak epidemiology, individual screening and contact tracing during the COVID-19 pandemic.

Anderson and colleagues discuss the progress and challenges of using mRNA for vaccines and immunotherapy.

Regev, Theis and colleagues outline the challenges and concepts of the analysis of spatial transcriptomics data.

This Review provides an overview of existing studies using single-cell technologies to provide insights over the immune responses and molecular mechanisms at work in COVID-19.

Au and colleagues outline the field of nanopore sequencing.

Chong et al. review how the integration of mass spectrometry with proteogenomic approaches can identify noncanonical antigens.

As the number of single-cell experiments with multiple data modalities increases, Argelaguet and colleagues review the concepts and challenges of data integration.

As CRISPR therapies move into clinical testing, David Schaffer and colleagues review a raft of different delivery technologies being road tested to address cargo capacity limitations, maximize potency, minimize off-target effects and avoid immunogenicity.

A growing arsenal of CRISPR-based tools enables increasingly sophisticated genome editing applications.

Hanna and Doench review the computational methods and tools that have become indispensable for planning and analyzing CRISPR experiments.