PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Cell Cycle Arrest in Different Cancer Cell Lines “Liver, Breast, and Colon” Induce Apoptosis under the Influence of the Chemical Content of Aeluropus lagopoides Leaves Extracts
Version 1
: Received: 14 September 2018 / Approved: 18 September 2018 / Online: 18 September 2018 (11:40:12 CEST)
How to cite:
Saleh, K. A.; Asiri, T. H.; Elbehairi, S. I.; Alshehry, M. A.; Elfaifi, M. Y.; Al-Ghazzwi, A. M. Cell Cycle Arrest in Different Cancer Cell Lines “Liver, Breast, and Colon” Induce Apoptosis under the Influence of the Chemical Content of Aeluropus lagopoides Leaves Extracts. Preprints2018, 2018090347. https://doi.org/10.20944/preprints201809.0347.v1
Saleh, K. A.; Asiri, T. H.; Elbehairi, S. I.; Alshehry, M. A.; Elfaifi, M. Y.; Al-Ghazzwi, A. M. Cell Cycle Arrest in Different Cancer Cell Lines “Liver, Breast, and Colon” Induce Apoptosis under the Influence of the Chemical Content of Aeluropus lagopoides Leaves Extracts. Preprints 2018, 2018090347. https://doi.org/10.20944/preprints201809.0347.v1
Saleh, K. A.; Asiri, T. H.; Elbehairi, S. I.; Alshehry, M. A.; Elfaifi, M. Y.; Al-Ghazzwi, A. M. Cell Cycle Arrest in Different Cancer Cell Lines “Liver, Breast, and Colon” Induce Apoptosis under the Influence of the Chemical Content of Aeluropus lagopoides Leaves Extracts. Preprints2018, 2018090347. https://doi.org/10.20944/preprints201809.0347.v1
APA Style
Saleh, K. A., Asiri, T. H., Elbehairi, S. I., Alshehry, M. A., Elfaifi, M. Y., & Al-Ghazzwi, A. M. (2018). Cell Cycle Arrest in Different Cancer Cell Lines “Liver, Breast, and Colon” Induce Apoptosis under the Influence of the Chemical Content of <em>Aeluropus lagopoides</em> Leaves Extracts. Preprints. https://doi.org/10.20944/preprints201809.0347.v1
Chicago/Turabian Style
Saleh, K. A., Mohammad Yahya Elfaifi and Adel Mahmoud Al-Ghazzwi. 2018 "Cell Cycle Arrest in Different Cancer Cell Lines “Liver, Breast, and Colon” Induce Apoptosis under the Influence of the Chemical Content of <em>Aeluropus lagopoides</em> Leaves Extracts" Preprints. https://doi.org/10.20944/preprints201809.0347.v1
Abstract
Natural product especially secondary metabolites that produced by plants under the stressed condition shown to have a different pharmacological impact. Aeluropus lagopoides is one of the typical halophyte plants survivals under stressed conditions. It has been used for wound healing and as a painkiller. The bioactivity and the chemical composition of this plant have poorly investigated. Consequently, chemical components of A. lagopoides leaves were extracted using hexane (nonpolar), ethyl acetate (semi-polar), n-butanol (polar) to extract the most extensive variety of metabolites. The cytotoxicity and anticancer impact of extracted secondary metabolites evaluated against breast (MCF-7), colon (HCT-116), and liver (HepG2) cancer cell lines using SRB test. The mechanism of action verified by observing the appearance of apoptotic bodies using the fluorescent microscope while their antiproliferative impact had been evaluated using flow cytometer. Results revealed that secondary metabolites extracted using hexane and ethyl acetate were having the highest cytotoxicity and thus anticancer activity effect on HepG2 with IC50 (24.29 ± 0.85, 11.22 ± 0.679 µg/mL) respectively. Where apoptotic bodies observed, flow cytometer results exhibited that secondary metabolites can inhibit cell cycle in G0/G1 phase. Accordingly, A. lagopoides hexane and ethyl acetate extracts may consider as a candidate anti-cancer drug.
Keywords
Aeluropus lagopoides; HepG2; HCT-116; MCF-7; apoptosis; chemical composition
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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