Review
Version 1
Preserved in Portico This version is not peer-reviewed
Development Perspective of Bioelectrocatalysis-based Biosensors
Version 1
: Received: 19 July 2020 / Approved: 20 July 2020 / Online: 20 July 2020 (08:16:47 CEST)
A peer-reviewed article of this Preprint also exists.
Adachi, T.; Kitazumi, Y.; Shirai, O.; Kano, K. Development Perspective of Bioelectrocatalysis-Based Biosensors. Sensors 2020, 20, 4826. Adachi, T.; Kitazumi, Y.; Shirai, O.; Kano, K. Development Perspective of Bioelectrocatalysis-Based Biosensors. Sensors 2020, 20, 4826.
Abstract
Bioelectrocatalysis provides the intrinsic catalytic-functions of redox enzymes to non-specific electrode reactions and is the most important and basic concept for biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multi-analyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
Keywords
current-potential curve; multi-enzymatic cascades; multi-analyte detection; mass-transfer-controlled amperometric response; potentiometric coulometry
Subject
Chemistry and Materials Science, Electrochemistry
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Commenter: Alfons Felice
The commenter has declared there is no conflict of interests.
I am Alfons, I have a background in biochemistry and biotechnology and work in the field of direct electron transfer based biosensors in the University Spin-off DirectSens in Vienna Austria.
Thank you very much for this stimulating contribution to the field of Direct Electron Transfer based bioanalytical devices. I got introduced to novel systems previously not known to me and increased my knowledge in the field. However, some parts are hard to read because of some unusual English expressions. For example: "Most of redox-enzymatic reactions can be coupled with electrode reactions by way of redox mediators " could be replaced by "Most of redox-enzymatic reactions can be coupled with electrode reactions via redox mediators " Professional language editing will improve this issue.
Further the manuscript shows, that the authors to have strong background in the field of electrochemistry, while the sections explaining biochemical reactions are less profound. An illustrativ example can be found in the introduction: These metallic ion cofactors undergo electron transfers. Therefore, flavin and quinone cofactors and Mocos have very important roles to couple the hydride ion transfer system and the electron transfer system. These metallic ion cofactors undergo electron transfers. Therefore, flavin and quinone cofactors and Mocos have very important roles to couple the
hydride ion transfer system and the electron transfer system.
Discussion of this manuscript with colleagues with background in biochemistry would further improve the quality.
In conclusion i would like to thank you and the group around Prof. Kano for your very active contribution to the field.