Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins

Gloria I. Giraldo-Calderón; Arley Calle-Tobon; Paula Rozo-Lopez; Tonya M. Colpitts; Yoonseong Park; Guillermo L. Rua-Uribe; Berlin Londono-Renteria

doi:10.20944/preprints202008.0231.v1

Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 9 August 2020 / Approved: 10 August 2020 / Online: 10 August 2020 (03:13:56 CEST)

Aedes aegypti is the primary mosquito vector of several human arboviruses including dengue virus (DENV). Vector control is the principal intervention to decrease the transmission of these viruses. The characterization of molecules involved in the mosquito physiological responses to blood-feeding may help to identify novel targets useful in the design of effective control strategies. In this study, we evaluated the in vivo effect of feeding adult female mosquitoes with human blood containing either heat-inactivated (IB), normal serum (NB), and RNA-seq based transcript expression was compared against sugar-fed (SF) mosquitoes. In the in vitro experiments, we treated Aag2 cells with a recombinant version of the complement proteins (hC3 or hC5a) and compared transcript expression to untreated control cells after 24h. The transcript expression analysis revealed that human complement proteins modulate approximately 2,300 transcripts involved in multiple biological functions, including the immune system. We also found 161 up-regulated and 168 down-regulated transcripts differentially expressed when hC3 and hC5a were compared against the control untreated cells. We conclude that active human complement induces significant changes in the transcriptome of Ae. aegypti mosquitoes, which can influence the infective capacity of pathogens ingested during blood meals.

human complement; Aedes aegypti; blood-meal; hC3; hC5a

Biology and Life Sciences, Insect Science

Comment 1

Received: 12 September 2020
Commenter:

Sergey Pushkin

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The commenter has declared there is no conflict of interests.

Comment: A very interesting and valuable article. It is important to a wide range of readers dealing with the transmission of viruses by mosquitoes. I hope that the article will be published soon, and will find a response from colleagues. Regards, Sergey Pushkin

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Response 1 to Comment 1

Received: 14 September 2020
Commenter:

Berlin L. Londono

The commenter has declared there is no conflict of interests.

Comment: Thanks a lot, Sergey for the positive feedback! The peer-reviewed final article is now available online on the IJMS website. All the best, B.

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Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins

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