Millán-Oropeza, A.; Blein-Nicolas, M.; Monnet, V.; Zivy, M.; Henry, C. Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes2022, 10, 2.
Millán-Oropeza, A.; Blein-Nicolas, M.; Monnet, V.; Zivy, M.; Henry, C. Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes 2022, 10, 2.
Millán-Oropeza, A.; Blein-Nicolas, M.; Monnet, V.; Zivy, M.; Henry, C. Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes2022, 10, 2.
Millán-Oropeza, A.; Blein-Nicolas, M.; Monnet, V.; Zivy, M.; Henry, C. Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes 2022, 10, 2.
Abstract
In proteomics, it is essential to quantify proteins in absolute terms if we wish compare results among studies and integrate high-throughput biological data into genome-scale metabolic models. While labeling target peptides with stable isotopes allows protein abundance to be accurately quantified, the utility of this technique is constrained by the low number of quantifiable proteins that it yields. Recently, label-free shotgun proteomics has become the “gold standard” for carrying out global assessments of biological samples containing thousands of proteins. However, this tool must be further improved if we wish to accurately quantify absolute levels of proteins. Here, we used different label-free quantification techniques to estimate absolute protein abundance in the model yeast Saccharomyces cerevisiae. More specifically, we evaluated the performance of seven different quantification methods, based either on spectral counting (SC) or extracted-ion chromatogram (XIC), which were applied to samples from five different proteome backgrounds. We also compared the accuracy and reproducibility of two strategies for transforming relative abundance into absolute abundance: a UPS2-based strategy and the total protein approach (TPA). This study mentions technical challenges related to UPS2 use and proposes ways of addressing them, including utilizing a smaller, more highly optimized amount of UPS2. Overall, three SC-based methods (PAI, SAF, and NSAF) yielded the best results because they struck a good balance between experimental performance and protein quantification.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
