Version 1
: Received: 6 March 2023 / Approved: 13 March 2023 / Online: 13 March 2023 (02:18:00 CET)
How to cite:
Oliveira, A. F. D.; Machado, R. B.; Ferreira, A. M.; Sena, I. D. S.; Silveira, M. E.; Almeida, A. M. S. D.; Braga, F. S.; Rodrigues, A. B. L.; Bezerra, R. M.; Ferreira, I. M.; Florentino, A. C. Copper-Contaminated Substrate Biosorption by Penicillium sp. Isolated From Kefir Grains. Preprints2023, 2023030204. https://doi.org/10.20944/preprints202303.0204.v1
Oliveira, A. F. D.; Machado, R. B.; Ferreira, A. M.; Sena, I. D. S.; Silveira, M. E.; Almeida, A. M. S. D.; Braga, F. S.; Rodrigues, A. B. L.; Bezerra, R. M.; Ferreira, I. M.; Florentino, A. C. Copper-Contaminated Substrate Biosorption by Penicillium sp. Isolated From Kefir Grains. Preprints 2023, 2023030204. https://doi.org/10.20944/preprints202303.0204.v1
Oliveira, A. F. D.; Machado, R. B.; Ferreira, A. M.; Sena, I. D. S.; Silveira, M. E.; Almeida, A. M. S. D.; Braga, F. S.; Rodrigues, A. B. L.; Bezerra, R. M.; Ferreira, I. M.; Florentino, A. C. Copper-Contaminated Substrate Biosorption by Penicillium sp. Isolated From Kefir Grains. Preprints2023, 2023030204. https://doi.org/10.20944/preprints202303.0204.v1
APA Style
Oliveira, A. F. D., Machado, R. B., Ferreira, A. M., Sena, I. D. S., Silveira, M. E., Almeida, A. M. S. D., Braga, F. S., Rodrigues, A. B. L., Bezerra, R. M., Ferreira, I. M., & Florentino, A. C. (2023). Copper-Contaminated Substrate Biosorption by Penicillium sp. Isolated From Kefir Grains. Preprints. https://doi.org/10.20944/preprints202303.0204.v1
Chicago/Turabian Style
Oliveira, A. F. D., Irlon Maciel Ferreira and Alexandro Cezar Florentino. 2023 "Copper-Contaminated Substrate Biosorption by Penicillium sp. Isolated From Kefir Grains" Preprints. https://doi.org/10.20944/preprints202303.0204.v1
Abstract
This work aimed to carry out a bioremediation study to evaluate the resistance of Penicillium sp. isolated from kefir grains for the treatment of copper. The fungal culture medium was prepared using a 2% malt-agar medium at pH 7.0 in which Penicillium sp. was inoculated. Penicillium sp. growing in a liquid medium showed a decrease in biomass in presence of Cu(NO3)2 (800 mg.L-1), suggesting that the metal impacts the growth rate of the fungus. Moreover, the combined factors of pH and the presence of the inorganic contaminant impacted the radial growth of the fungus, causing inhibition of 73% at pH 4.0, 75% at pH 7.0, and 77% at pH 9.0 in liquid medium compared to control. However, images obtained with scanning electron microscopy showed the integrity of the fungus cell, even at high doses of copper in the medium. Therefore, it can be concluded that Penicillium sp. isolated from kefir grain can bioremediate the environment and that the harmful effects of heavy metals can be minimized as a result biosorption. Although the growth of Penicillium sp. is inhibited, such retardation requires high doses of copper nitrate, thus ensuring the use of this microorganism for protection against the harmful effects of non-essential copper in the environment.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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