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Paper 6 June 2000

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Answer guide for O Level Biology (5090/6) – Alternative to Practical June 2000

1(a)(i) Table 1.1

number of flies
male 8
female 8
normal wing 12
vestigial wing 4
3 correct = 1 ; 4 correct = 2 ; 2
(ii) 3:1; 1
(iii) normal wing ;
because normal (winged) parents had some vestigial offspring /
reference to heterozygous / Nn ; 2
(b) [Either as diagram or Punnet square]
parents / male & female Nn x Nn ;
gametes N &n ;
NN Nn Nn nn ;
phenotypes shown ; 4
(c) Appearance: (all) vestigial ;
Explanation: no dominant (allele) present / homozygous recessive ;
therefore vestigial shows ; 3

2(a)(i) [Comparative statements or contrasting features]


Seedling A:
More growth / longer / larger as a whole ;
Has root hairs ;
Curved / larger / longer stem / shoot / plumule ;
Plumule / stem split / double ;
Root thicker/larger/longer ;
Seed / cotyledon smaller ; up to 2 2
(ii) Reasonable reference to conditions:
light / water / temperature / nutrients / NO3 / soil / O2 / air ;
(CO2, auxins, warmth, moisture not sufficient) 1
(b)(i) 28 mm / 2.8 cm ;
(ii) 56 mm, ; 2
(c)(i) A = 40 - 46 ;
B = 21 - 25 ;
(ii) e.g. 17 x 100 = 74%
23 3

Page 1 of 3 © UCLES 2002


Answer guide for O Level Biology (5090/6) – Alternative to Practical June 2000

3(a)(i) Iris drawn with wider gap ;


(ii) Lens drawn with greater bulge ; 2
(b)(i) Table 3.2 all correct ; ;
[one mistake ; 1]

Distance ruler fell Number of classmates


9 1
10 2
11 4
12 5
13 4
14 2
15 1
2
(ii)

9 10 11 12 13 14 15
distance (ruler fell) / cm
1. x axis labelled – central units
2. y axis labelled – on line (number of classmates / frequency)
3. Accurate plotting (columns joined or separate)
4. Clearly ruled plot, good size on grid [min. 6cm wide, 5cm high] 4
(Correct line graph loses two marks)
(iii) alcohol /tiredness / drugs ; Not disability, alertness 1
(iv) move to right (answer must refer to the chart) ; 1
(v) (in) reflex action (touching hot object / which this experiment was
not) ;
impulse travels -shorter distance / only to spinal cord; 2

Page 2 of 3 © UCLES 2002


Answer guide for O Level Biology (5090/6) – Alternative to Practical June 2000

4(a) same / specified temperature ;


same / specified material ;
same / specified volume of water / reference to dilution /
concentration ;
same / specified volume /amount of washing powder ;
same amount / size of stain ; [up to 4] 4
(b) In the appropriate sequence:
1. In test-tube put protein/albumen with enzyme & pH (buffer) ;
2. Take out test samples (with Biuret) / at intervals / repeat until no
reaction / protein disappeared ;
3. Repeat (or set up initially) for different pH levels;
4. (somewhere in answer) Biuret + protein gives mauve/violet/
purple colour; (i.e. the test described) 4

Page 3 of 3 © UCLES 2002

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