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    (-1/KM) 1/vmax KM - 0.83333333 0 Vmax1 0 166.666667 Vamx2 0 250 Vmax3 0 500 500 600

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    elfbilent
    The document contains data from several enzyme kinetics experiments measuring variables such as substrate concentration, reaction rate, and enzyme concentration. Specifically, it includes tables with columns of kinetic data that were plotted and fitted with linear and non-linear regression lines. The best-fit lines were used to calculate kinetic parameters such as the Michaelis-Menten constant KM and maximum reaction rate Vmax for the enzyme.

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    Attribution Non-Commercial (BY-NC)
    Download as XLS, PDF, TXT or read online from Scribd

    (-1/KM) 1/vmax KM - 0.83333333 0 Vmax1 0 166.666667 Vamx2 0 250 Vmax3 0 500 500 600

    Uploaded by

    elfbilent
    10 views6 pages
    The document contains data from several enzyme kinetics experiments measuring variables such as substrate concentration, reaction rate, and enzyme concentration. Specifically, it includes tables with columns of kinetic data that were plotted and fitted with linear and non-linear regression lines. The best-fit lines were used to calculate kinetic parameters such as the Michaelis-Menten constant KM and maximum reaction rate Vmax for the enzyme.

    Original Title

    sinav_yanitlari

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    © Attribution Non-Commercial (BY-NC)

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    The document contains data from several enzyme kinetics experiments measuring variables such as substrate concentration, reaction rate, and enzyme concentration. Specifically, it includes tables with columns of kinetic data that were plotted and fitted with linear and non-linear regression lines. The best-fit lines were used to calculate kinetic parameters such as the Michaelis-Menten constant KM and maximum reaction rate Vmax for the enzyme.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLS, PDF, TXT or read online from Scribd
    Download as xls, pdf, or txt
    10 views6 pages

    (-1/KM) 1/vmax KM - 0.83333333 0 Vmax1 0 166.666667 Vamx2 0 250 Vmax3 0 500 500 600

    Uploaded by

    elfbilent
    The document contains data from several enzyme kinetics experiments measuring variables such as substrate concentration, reaction rate, and enzyme concentration. Specifically, it includes tables with columns of kinetic data that were plotted and fitted with linear and non-linear regression lines. The best-fit lines were used to calculate kinetic parameters such as the Michaelis-Menten constant KM and maximum reaction rate Vmax for the enzyme.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLS, PDF, TXT or read online from Scribd
    Download as xls, pdf, or txt
    of 6

    (-1/KM) 1/Vmax

    Km -0.83333333 0
    Vmax1 0 166.666667 600
    Vamx2 0 250
    Vmax3 0 500 500

    400

    300

    200

    100

    0
    -0.9 -0.8 -0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1 0

    (-1/KM) 1/Vmax
    -0.83333333 0
    0 0.004
    600

    500
    Row 3
    400
    Linear (Row 3)
    Row 4
    300
    Linear (Row 4)
    200 Row 5
    Linear (Row 5)
    100

    0
    0.4 -0.3 -0.2 -0.1 0
    [S] nmol/100 µl Reaction Volume dpm Recovered in Product Formed 1/[S]
    0.01 1.00
    0.20 9.00 5.00
    0.40 14.00 2.50
    0.50 16.00 2.00
    0.75 17.50 1.33
    1.00 19.00 1.00

    0.1200
    20.00
    f(x) = 0.014789363930892 x
    18.00 0.1000
    16.00
    14.00 0.0800

    12.00
    0.0600
    10.00 Colum
    nB
    8.00
    0.0400
    6.00
    4.00 0.0200
    2.00
    0.00 0.0000
    0.00 0.20 0.40 0.60 0.80 1.00 1.20 0.50 1.00 1.50 2.00
    1/V

    0.1111
    0.0714
    0.0625
    0.0571
    0.0526

    f(x) = 0.014789363930892 x + 0.035961329089538

    Column D
    Linear
    (Column D)
    Linear
    (Column D)
    Linear
    (Column D)

    0 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50
    [S] µM V (µmol/min) V (µmol/min) with 25 nM 1/S
    0.4 0.22 0.21 2.5
    0.67 0.29 0.26 1.49253731
    1 0.32 0.3 1
    2 0.4 0.36 0.5

    KM 0.49163386 Vmax 0.492125984251969

    5
    4.5 f(x) = 0.986475092116431 x + 2.32522961673703
    f(x) = 0.99967718292318 x + 2.03199154473263
    4
    3.5
    Column F
    3 Linear
    (Column F)
    2.5 Column G
    2 Linear
    (Column G)
    1.5 Linear
    (Column G)
    1
    0.5
    0
    0 0.5 1 1.5 2 2.5 3
    1/V 1/VI
    4.54545455 4.76190476
    3.44827586 3.84615385
    3.125 3.33333333
    2.5 2.77777778

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