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    Bacterial Growth

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    Iqra Noor
    This document discusses methods for measuring bacterial growth and environmental factors that affect growth. It describes three main methods: direct microscopic count, determination of turbidity, and viable cell count/colony counting. It then explains several environmental factors that impact bacterial growth, including temperature, pH, oxygen, carbon dioxide, osmotic pressure, hydrostatic pressure, radiation, salinity, and moisture.

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    Bacterial Growth

    Uploaded by

    Iqra Noor
    165 views5 pages
    This document discusses methods for measuring bacterial growth and environmental factors that affect growth. It describes three main methods: direct microscopic count, determination of turbidity, and viable cell count/colony counting. It then explains several environmental factors that impact bacterial growth, including temperature, pH, oxygen, carbon dioxide, osmotic pressure, hydrostatic pressure, radiation, salinity, and moisture.

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    Bacterial+Growth

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    This document discusses methods for measuring bacterial growth and environmental factors that affect growth. It describes three main methods: direct microscopic count, determination of turbidity, and viable cell count/colony counting. It then explains several environmental factors that impact bacterial growth, including temperature, pH, oxygen, carbon dioxide, osmotic pressure, hydrostatic pressure, radiation, salinity, and moisture.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    165 views5 pages

    Bacterial Growth

    Uploaded by

    Iqra Noor
    This document discusses methods for measuring bacterial growth and environmental factors that affect growth. It describes three main methods: direct microscopic count, determination of turbidity, and viable cell count/colony counting. It then explains several environmental factors that impact bacterial growth, including temperature, pH, oxygen, carbon dioxide, osmotic pressure, hydrostatic pressure, radiation, salinity, and moisture.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    You are on page 1of 5

    Measurement of Bacterial Growth

    1. Direct Microscopic Count

    2. Determination of Turbidity

    3. Viable Cell Count / Colony Counting


    Page 1 of 5

    Indirect Methods- Plate Count Dilute the sample and plate a known volume; incubate count the number of colonies 1 colony = 1 cell Calculate cells/ml adjusting for dilution

    Advantages/disadvantages Direct count and turbidimetry may count dead cells Viable cell count do not Environmental Factors That Affect Growth
    Page 2 of 5

    Temperature pH Oxygen Carbon Dioxide Osmotic Pressure Hydrostatic Pressure Radiation Salinity

    Temperature pH Acidophiles- optimum below pH 5.5 Neutrophiles- optimum at pH 6-8 Alkalophiles- optimum above pH 8 Molecular Oxygen (O2)

    Psychrophiles - optimum less than 20C Mesophiles- optimum 20-45C Thermophiles- optimum 45-80C Extreme Thermophiles- optimum 85+C

    Microbe vary greatly in sensitivity to O2 Aerobes- microbes which require O2. Anaerobes- microbes which DO NOT utilize O2 AND are KILLED by O2
    Page 3 of 5

    Facultative Microbes- microbes which can grow in presence OR absence of O2 Microaerophiles - required 3-15% O2

    Carbon Dioxide Capnophiles - optimum 3-10% CO2 Many microaerophiles are also capnophiles Cultured in a candle jar

    Osmotic Pressure Interior osmotic pressures must be balanced against exterior osmotic pressures Each microorganism displays an optimum salt concentration for growth Halophiles- require high concentrations of of NaCl

    Page 4 of 5

    Halo-tolerant- will grow in high NaCl concentrations but grow best at lower levels Hydrostatic Pressure o Barophiles- optimum hydrostatic pressure for growth of up to 1000 atm o Normal Pressure = 1 atm o Present in deep sea, oceans at depths Radiation Survive up to 10,000 Grays 5 grays can kill a man, Deinococcus radiodurans Help in radioactive waste disposal

    Moisture Some amount of water is required Dehydration can kill bacteria Spores survive desiccation

    Page 5 of 5

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