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    Millon Test

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    alxxndraromero
    The document describes a Millon test used to detect the presence of proteins. The test involves adding Millon's reagent, composed of mercuric nitrate, nitric acid and water, to samples of egg albumin, gelatin and water. For egg albumin and gelatin, the results were a light red or brick red solution and precipitate, indicating the presence of proteins. Water showed no color change. The test works because Millon's reagent reacts with the amino acid tyrosine, producing a red color, and tyrosine is present in proteins but not other substances.

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    Millon Test

    Uploaded by

    alxxndraromero
    546 views2 pages
    The document describes a Millon test used to detect the presence of proteins. The test involves adding Millon's reagent, composed of mercuric nitrate, nitric acid and water, to samples of egg albumin, gelatin and water. For egg albumin and gelatin, the results were a light red or brick red solution and precipitate, indicating the presence of proteins. Water showed no color change. The test works because Millon's reagent reacts with the amino acid tyrosine, producing a red color, and tyrosine is present in proteins but not other substances.

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    Millon test

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    The document describes a Millon test used to detect the presence of proteins. The test involves adding Millon's reagent, composed of mercuric nitrate, nitric acid and water, to samples of egg albumin, gelatin and water. For egg albumin and gelatin, the results were a light red or brick red solution and precipitate, indicating the presence of proteins. Water showed no color change. The test works because Millon's reagent reacts with the amino acid tyrosine, producing a red color, and tyrosine is present in proteins but not other substances.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    546 views2 pages

    Millon Test

    Uploaded by

    alxxndraromero
    The document describes a Millon test used to detect the presence of proteins. The test involves adding Millon's reagent, composed of mercuric nitrate, nitric acid and water, to samples of egg albumin, gelatin and water. For egg albumin and gelatin, the results were a light red or brick red solution and precipitate, indicating the presence of proteins. Water showed no color change. The test works because Millon's reagent reacts with the amino acid tyrosine, producing a red color, and tyrosine is present in proteins but not other substances.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Submitted by: Date Submitted: November 26, 2012

    Alexandra Regilyne M. Romero


    Samantha Araullo
    MILLON TEST FOR PROTEIN
    I. Introduction
    The word protein is derived from the Greek word proteios, which means of
    primary importance. In fact, proteins plays an important role in all biochemical and
    physiological body processes; they act as enzymes, hormones, receptors, antibodies and are
    required for the structural integrity of cells.

    II. Procedure
    Add 10 drops of the sample to the test tube (Sample: Egg albumin, 2% gelatin,
    and water)
    Add 10 drops of Millon reagent to 10 drops of sample tube
    Mix well and heat on water bath
    Observe and note the change in color

    III. Test reagent compound
    Reagent: Millon Reagent
    Composition: Mercuric Nitrate (10415-75-5) 38%, Nitric Acid (7697-370-2 ) 18%, and
    Water (7732-18-5) 44%.

    IV. Results

    RESULTS
    Egg Albumin + Millon reagent Light red solution and precipitate
    Gelatin + Millon reagent Brick red solution and recipitate
    Water + Millon reagent Colorless solution

    V. Explanation
    Millon's reagent (Hg/HNO3) gives positive results with proteins containing the
    phenolic amino acid tyrosine. Tyrosine is the only amino acid that contains a phenol
    group on which a hydroxyl group is attached. Millon's reagent reacts with the tyrosine
    residues and gives a positive result of red coloration to the solution.




    Tyrosine C9H11NO3



    VI. References:
    http://en.wikipedia.org/wiki/Millon's_reagent
    http://www.flickr.com/photos/gabriellevelasco/sets/72157624546602231/detail/
    Amino acids.pdf

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