Improvement and Management of Horticultural Crops
Improvement and Management of Horticultural Crops
Improvement and Management of Horticultural Crops
37
Improvement and Management of Horticultural Crops
FRUIT CROPS
Mango
Mango superior trees of different varieties were identified and
scion sticks collected at IIHR, Bangalore. Eighteen accessions in
mango were characterized morphologically. Mangifera odorata
recorded maximum TSS (21.7 Brix). The pulp recovery was found
83 and 81% in mango Keraligoa and Khudadath (variant),
respectively. Distinct physiological shift of seed into germination
in Kolar district in Karanataka indicated zinc (1317 ppm) and
boron (3445 ppm) deficiencies. Zinc (1517 ppm), manganese
(2832 ppm) and boron (42 ppm) deficiencies were also found in
Krishnagiri. The studies on pest risk analysis of Bactrocera caryeae
showed that it is restricted to west coast of India and major mango
belts of South India are free from it. Extensive surveys carried out
for mango fruit fly and stone weevil infestation in South India on
Banganapalli showed that Krishna, Mehaboobnagar/Rangareddy
districts of Andhra Pradesh, were free from these pests.
At CISH, Lucknow, plastic mulch enhanced flowering in mango.
The response of black plastic mulch (100 ) was evaluated against
root growth, nutrient status of soil and plant, flowering and yield
of mango Chausa. Mulching was done during flower-bud
differentiation (OctoberNovember) stage. The mulch stimulated
the lateral root growth particularly in nutrient rich upper soil layer.
However, there was no difference in dry weight of roots. The data
on nutritional status revealed the improvement in levels of Ca
(521.05 ppm), Zn (0.93 ppm), Cu (1.61 ppm), Mn (6.66 ppm)
and Fe (4.90 ppm) in mulched soil as compared to control (440.95,
0.75, 0.66, 4.52 and 3.6 ppm, respectively). Among different
nutrients, phosphorus (0.21%) and potash (0.8%) were found
significantly higher in leaves of mulched plants in comparison to
control (0.15 and 0.56%). Enhancement in flowering and yield
(3870 and 4060%) were also recorded in off and on year
because of mulch. Increase in these attributes may be associated
with increase in root area along with enhanced ability of tree to
take more nutrients particularly phosphorus and potassium from
soil under mulched condition.
Single application of five bacterial antagonists isolated from
mango pathosystem and leaf based organic liquid pesticides were
found effective against mango bacterial canker disease. These
organisms were identified as species of Bacillus, Pseudomonas
and Acenetobacter.
Under All India Coordinated Research Project on Sub-Tropical
Fruits, Dudhiya Malda performed very well followed by Dholikothi
Maldah at Sabour, Bappakai and Sensation at Sangareddy, Bangalore
Goa followed by Zafrani Gola at Pantnagar and Keraligoa,
Khudadath and Navneetham at IIHR, Bangalore. Peddapur-16
seedling at Sangareddy and collection Nos. 7/80, and 10/80 were
found promising at Sabour. Two clones, T19 and T20, of Himsagar
Eighteen accessions of mango were characterized
T 19 and T 20, clones of mango Himsagar, were
promising
Salem and Javori, clones of mango Bangalora wre
promising
Hybrid H 311 gave maximum fruit yield
Rejuvenation of mango orchards was done
mode allows the seed to act as a stronger physiological sink,
enabling it to draw water and other nutrients from adjoining
mesocarp resulting in spongy tissue. The survey of mango orchards
of Krishna district for nutrient status indicated that the age of
orchards decides the deficiency of micronutrients and their
management for yield and quality. The correction of micronutrient
disorders in mango Banganapalli in Krishna districts recorded 12
18% increase in yield. The causes of low yield in mango orchards
Fruit quality of treated tree
DARE/ICAR ANNUAL REPORT 20062007
38
were found promising for high yield at Mohanpur and clone Rati
Banganpalli having cluster bearing habit, early Baneshani having
early maturity, Pedda Benishan and Allahabad Benishan bearing
bigger sized fruits than Banganpalli performed well at Sangareddy.
Similarly, two clones of Bangalora, i.e. Salem and Javori having big
fruits (300500 g) were found promising at Periyakulam. Hybrid H-
311 (Alphonso Neelum) recorded maximum yield and was found
free from spongy tissue disorder at Vengurle. Neeleshwari and
Neeleshan were found promising at
Paria.
In planting system-cum-high-density planting trial, double
hedgerow system of planting gave significantly higher yield at most
of the centres. In pruning trial for high- density planting,
maximum number of fruits and yield were recorded in the trees
pruned on alternate limbs after harvesting with application of
paclobutrazol at Sangareddy, whereas at Paria and Vengurle,
heading back of 20 cm terminal shoot biennially with application
of paclobutrazol during rest period gave higher yield. Heading back
of branchlet at 50 cm level with the application of paclobutrazol
during rest period gave higher yield in trees planted at normal
distance at Vengurle. Rejuvenation of overcrowded orchards
(heading back up to crowded branchlets and centre opening) along
with use of paclobutrazol during the rest period gave maximum
cumulative yield of last seven years at Sangareddy and Pantnagar.
Maximum hopper population was recorded on panicles during
flowering stage, i.e. second fortnight of March (65.5) followed by
that on trunk of tree (57.1) in first fortnight of April and on leaves
in first fortnight of July (58.6) at Mohanpur. Higher activity of
hopper was observed during flowering stage, while thrips had two
peaks (OctoberNovember and MarchMay) coinciding with new
flush period at Paria. Three sprayings of Imidacloprid and
Endosulphon starting first spray of Imidacloprid (0.005%) at
panicle emergence followed by the rest two sprays of Endosulphan
(0.07%) at 15 days and 21 days intervals proved highly effective by
reducing hopper population by 20.07% with highest yield of
346.6kg/tree at Sabour. Number of fruits set at marble and
harvesting stages were significantly higher in treated plots sprayed
with Imidacloprid (0.005%) at panicle emergence than in
untreated control at AES, Paria. Module-II (Imidacloprid (0.005%),
NSKE (5%) and Endosulphan (0.07%) was highly effective by
resulting in lowest survival of hopper population (8.64%) and
maximum fruit yield (259 kg/tree) in mango Langra at Sabour.
Banana
Forty-six exotic accessions of banana were introduced from
Belgium at NRC for Banana, Trichy. Phylogenetic relationship
among 45 AB diploid accessions was analysed using microsatellite
markers. Embryogenic cell suspensions of banana Nendran
(French Plantain) and Ney Poovan were developed.Kanai Bansi,
M. balbisiana, Athiakol, Bhimkol and Attiakola were potential
resistant donors against burrowing and root lesion nematodes,
while Ankur was moderately resistant to root-knot nematode. Nattu
Poovan, Thella Chakkarakeli, Teraben, Mannan, Musa balbisiana,
Ladan, Erode Kai and Pisang Berlin were drought tolerant based
on leaf water retention capacity.
In Red Banana, plants grown under paired row planting system
with a population of 3,800 plants/ha recorded 29.5% more yield than
2,500 plants/ha. The fruits recorded highest TSS (23.5 Brix), total
sugars (20.96%), ascorbic acid (12.53 mg/100 g), peel carotene (3.02
mg/100 g) and low acidity (0.26%) under paired row planting.
In Rasthali, application of 15 kg rice husk ash + 80%
recommended NPK + 25 g VAM/plant recorded highest average
bunch weight of 12.3 kg with 30% increase over control (9.4 kg).
Under high soil pH (>8.5) condition, soil application of iron and
boron and foliar application of zinc increased bunch weight in Ney
Poovan banana. Fertilizer adjustment equations were developed
for optimum production of banana Rasthali.
For integrated pest management in banana, a semi-chemical
Eicosane evoked higher response (0.732 eV) to female antenna,
whereas cyclohexane responded to male antenna (0.292 eV).
Complete mortality of burrowing nematode was observed with
application of flower and root extracts of Tagetus erecta.
Applications of 30 g Trichoderma viride at planting, and 3 and 5
months after planting or in combination with Pseudomonas
fluorescens reduced nematode population significantly with
increased plant growth.
Primers, OPB1, OPB7 and OPB17, showed polymorphism and
differentiated different foc races by cluster analysis. Nit mutants
were generated for VCG grouping of different foc isolates. Presence
of VCGs 0124, 0125, and 0128 of foc in India has been confirmed.
Two of the rDNA-ITS sequences of foc have been deposited in the
NCBI genbank. Complete genome of banana streak virus (BSV)
was cloned, sequenced and deposited with the NCBI genbank
(Accession 859899). The viral genome was 6950 bp in length and
had three ORFs. This BSV sequence is more similar to BSV-OL and
GD sequences, 900 bp intergenic BSV sequence was cloned for
assessing the promoter activity. The BBTV cp gene were cloned into
an expression vector for producing recombinant coat protein.
The ACC oxidase gene was isolated and fused with CaMV 35S
promoter and terminator in anti-sense orientation to develop
Forty-six exotic accessions of banana wre added
Primers, OPB1, OPB7 and OPB17, showed polymorphism
Four distinct bacterial clones were isolated
A total of 1,1000 suckers of banana were supplied at
Arabhavi, 50,000 at Jorhat and 680 at Mohanpur
IMPROVEMENT AND MANAGEMENT OF HORTICULTURAL CROPS
39
transgenic banana with delayed ripening. This whole cassettes of
ACC oxidase in anti-sense orientation with respect to CaMV 35S
promoter was shuffled to binary vectors of pGreen and pCAMBIA.
Under AICRP on Tropical Fruits, four accessions of banana have
been catalogued for their morphological traits at Coimbatore. At
Kannara, 15 regenerated new accessions of banana were planted
for field evaluation. At Coimbatore, 20 crosses were made using
Manoranjitham and Karpooravalli as female parents and Rose,
YKM 5, Pisang Lilin and H-65 as male parents, which yielded 106
seeds. The hybrid progeny from different crosses were established
in field under Kannara conditions. At Kovvur, RAPD analysis
corroborated the genetic variability discovered by isoenzyme
analysis between Fusarium wilt resistant Silk banana Martman
from other susceptible members of group. The cultivar can directly
be deployed in Fusarium (race-1) endemic areas. Banana hybrid,
96/7 and H 212, tolerant to nematodes were proposed as pre-release
cultures at TNAU, Coimbatore. Application of fertilizers of
200 : 50 : 200 N, P
2
O
5
and K
2
O respectively/plant/crop along with
spraying of 2,4-D at 10 ppm gave higher yield during plant crop of
banana under Arabhavi conditions, while at Kannara, 2, 4-D (25
ppm) as bunch spray along with soil application of 200 g N and
foliar application of urea (2%) recorded yield increase in banana
Nendran. At Coimbatore, combined treatment of 300 g N and 300
g K
2
O with two post-shooting spraying of CPPU (4 ppm) gave
maximum bunch weight of 37.25 kg. The treatment propiconaole
(0.1%) + spraying of Bacillus subtilis @ 5 ml/litre controlled
Sigatoka leaf spot effectively under Arabhavi and Kannara. A total
of 1,100 suckers of banana were supplied at Arabhavi, 50,000 at
Jorhat and 680 at Mohanpur.
For molecular characterization of fruit fly species, molecular
differences in mitochondrial cytochrome oxidase I (mtCOI) gene
of three fruit fly specie, viz. Bactrocera dorsalis, B. correcta and
B. zonata (Tephritidae: Diptera) were carried out using mtCOI
specific primers. Sequence analysis of PCR amplified fragments
showed appreciable differences in nucleotide sequences of three
fruit fly species.
Four distinct bacterial clones were isolated from bacterial
contamination displaying in vitro cultures of banana Grand Naine
and single colony-purified and identified through 16SrDNA
sequence analysis. These organisms included Pantoea
agglomerans, Bacillus subtilis, Klebsiella variicola and
Staphylococcus epidermidis. All these endophytes appeared
detrimental to banana cultures overrunning them within 24
weeks of in vitro culturing.
Citrus
At NRC for Citrus, Nagpur, one early-maturing clone (N
2
) of
Nagpur mandarin (early-February) and another less-seeded clone
(N
4
) of less than 3 seeds/fruit were identified for evaluation at
farmers fields. In germplasm collection, 49 exotic germplasm
comprising 30 scions (14 of mandarin, 9 of sweet orange, 7 of
grapefruit and pummelo from USA, France, Japan and Niger) and
19 of rootstocks (mostly from USA, a few from Australia) and 532
indigenous collections have been maintained at Nagpur. Besides,
75 superior clones of Nagpur mandarin, 12 of acid lime and 5 of
Mosambi were under field evaluation. For micronutrient-use
efficiency in Nagpur mandarin, suboptimum Zn and Fe nutrition
are considered to be the global concerns for sustainable citrus
production. Better efficiency of soil applied Zn over foliar
application and vice-versa in case of Fe, was observed with respect
to response on flowering intensity, fruit setting, tree canopy volume,
Seventy-five clones of Nagpur mandarin, 12 of acid lime
and 5 of mosambi were evaluated
On selection each of sweet orange and pummelo were
collected
Use of Bioagents
In field release of bioagents against citrus insect pests, release
of Mallada boninensis @ 30 larvae/tree and Tamarixia
radiata @ 40 adults/tree resulted in 2830, 4245 and 23
26% reduction in blackfly, psylla and leaf miner, respectively.
fruit yield and quality indices. Higher movements of Zn through
xylem compared to Fe and of Fe through phloem in comparison to
Zn, were identified as the major distinguishing factor in
understanding the differential response between Zn and Fe
fertilization.
Continuous trenching between two rows of trees across the slope
(3.8%) conserves 3035% run-off and 2530% soil and available
nutrients (N, P, and K) in young Nagpur mandarin, producing
better plant growth. In bearing acid lime as well, continuous
trenching produced the best response conserving 3538% run-off
and 3235% soil and available N, P, and K in addition to raising
fruit yield by 18% with better fruit quality (43.2% juice) over
control (35.4% juice). Harvested rainwater from 3.1 ha of
catchments through a tank of 35 m 35 m 3 m size was
successfully recycled through drip irrigation with plastic mulch in
1 ha (288 plants) of 3-year-old Nagpur mandarin orchard.
Of the nine biopesticides tested, choicest biopesticides/new
molecules in their order of toxicity to blackfly and leaf miner were
found abamectin > spinosad > novaluron and to psylla and
spinosad > abamectin > novaluron to leaf minor. Oviposition
DARE/ICAR ANNUAL REPORT 20062007
40
preference by leaf miner, blackfly and psylla on Nagpur mandarin
revealed that citrus blackfly and leaf miner preferred 1520 and
510 days old leaves, respectively, whereas psylla preferred 5 days
old twig for egg laying.
Under AICRP on Tropical Fruits, one selection in sweet orange and
another in pummelo were collected and planted at Tirupati. Sweet
orange mosambi selections 2 and 4 and acid lime promising selection
RHR-L-124 continued to record their superiority for yield and fruit
quality at Rahuri. At Tinsukia, Khasi mandarin selection CRS-4
continued to be superior among 12 clonal selections. Addition of VAM
(500 g/plant) + PSB (100 g/plant) + Azospirillum (100 g/plant) +
T. harzianum (100 g/plant) to 100% RDF/plant/year produced
highest yield of sweet orange at Rahuri. Fifty-seven grafts of sweet
orange Mosambi and 315 seedlings of acid lime (Sai Sharbati) were
supplied to farmers at Rahuri. Virus-free kinnow was budded on 2,000
plants of Rough lemon at Ludhiana.
Grape
Eight exotic accessions of Vitis vinifera were introduced in
addition to collection of four wine grape accessions from private
winery at NRC for Grapes, Pune. The National Grape germplasm
collection now has 415 accessions. A total of 149 accessions were
characterised based on berry and bunch characters. Forty-four
accessions were characterised using RAPD and microsatellite
markers and genetic relationship among these accessions was
established.
Several hybrids of indigenous species and varieties with
introduced cultivars were found promising for early ripening and
quality traits. Germplasm was screened for resistance to thrips and
downy mildew and several accessions showing varying levels of
tolerance were identified, which can be used in breeding
programme to develop resistant commercial varieties. In
microsatellite analysis of a large number of downy mildew resistant
and susceptible grape accessions identified a marker which is
present in only susceptible accessions.
Thompson Seedless grafted on 110R produced significantly
more bunches and higher yield as compared to those grafted on
other rootstocks as well as own roots. Nutrient uptake pattern was
found to be different for different nutrients in grafted and own
rooted vines. Vines grafted on Dogridge and 110R accumulated
lower chlorides in tissues than those grafted on Salt Creek.The
shiny spot symptoms was found to be due to potassium deficiency.
Similarly, low potassium content was found to be associated with
marginal necrosis of leaf blade.
The IBA concentration was standardized for propagation of
grape rootstocks using hardwood cuttings. No adverse effect on
growth, yield and quality parameters were observed in nine years
old Tas-A-Ganesh grafted on Dogridge rootstock with different levels
of stock scion growth ratio (inverted bottleneck symptom).In
Thompson Seedless, maximum photosynthetic rate and internal
CO
2
was recorded during fruit-bud differentiation and full bloom
stages on 110R rootstock compared to other rootstocks. Stock scion
ratio of 0.91.0 was recorded in Tas-A-Ganesh when grafted on
110 R and B2/56 rootstocks, while it was 080.9 on Dogridge
indicating higher vigor inducing nature of Dogridge. However, yield
and number of bunches were maximum on 110 R followed by that
on Dogridge rootstock.
Increased root : shoot length ratio and water-use efficiency were
observed in most of the rootstocks at 50% moisture stress over
control. At 50% moisture stress, although Dogridge recorded
maximum root : shoot length ratio, 110 R showed maximum water-
use efficiency. Total phenolic compounds and individual class of
phenols like flavonoids, falvonols, flavon 3 ols were estimated
in Thompson Seedless grafted on different rootstocks at three
different berry growth stages. Preliminary investigation revealed
positive correlation between phenolic compositions and reduced
incidence of powdery mildew.
In Thompson Seedless, higher bud fruitfulness was recorded in
upward positioned shoots with maximum bud fruitfulness at 5
7th bud position. In Tas-A-Ganesh horizontal single cordon
training modification recorded higher yield than double cordon
system. Double stem four cordon system in Tas-A-Ganesh though
resulted in higher yield as compared to single stem and other
training modifications, however berry and bunch quality
characteristics were low in this treatment. In plastic covered Tas-A-
Ganesh girdling at 67 mm berry size resulted in improved
performance of vines with respect to berry weight, diameter and
length, total soluble solids and early berry setting. In Sharad
Seedless, girdling at 68 mm berry size resulted in higher berry
diameter, and berry and bunch weight. In Merlot and Sauvignon
A total of 149 accessions were characterized
A software for grape germplasm information system was
made operational
A prototype disease forecasting of software for powdery
mildew management in grape was developed
Statistical model was developed to predict powdery mildew
incidence in grape
Software in Grape Production
The software for grape germplasm information system was
made operational. Database for molecular data of grape
accessions was designed.
IMPROVEMENT AND MANAGEMENT OF HORTICULTURAL CROPS
41
Blanc maximum bud fruitfulness was recorded when spur pruned
at 5th and 4th bud respectively.
At reduced level of irrigation, vines raised on B2/56 performed
better than Dogridge and own roots. The cost : benefit ratio was
highest for B2/56 at 75% and 50% of recommended irrigation level.
Similarly, cost : benefit ratio of subsurface irrigation at 75% of
recommended level was on par with recommended level, thus
resulting in a saving of 25% irrigation water. In another
experiment, irrigation requirement could be reduced by 25% using
mulch and antistress, although the cost : benefit ratio of this
treatment was less than the recommended levels of irrigation.
A prototype disease forecasting software for powdery mildew
management was developed. The software takes daily weather data,
field data and generates diagnosis and recommends the choice
and dose of fungicides. The software was released and made
available to growers.
Several environmentally safe chemicals and new molecules were
tested and found promising for controlling powdery mildew. The
efficacy of Carbendazim was improved by adding citric acid @30
g/100 ltres of spray water. Biocontrol agent, Trichoderma was tested
for antagonism to Elsinoe ampelina and significant
mycoparasitism was observed.
Among several antagonistic microorgaisms isolated from grape
rhizoshere and endophytes, three were found to be promising for
the control of post-harvest decay and three were promising against
Botryodiploidea theobromae.
At Bangalore, three promising Thompson Seedless vines
producing loose bunches and bold berries were identified. Total
antioxidant capacity was significantly higher in Bangalore Blue
grape seed followed by peel due to higher total phenols in seeds
and anthocyanins and phenols in peel when compared to whole
fruit. Raisins from Thompson Seedless grape had higher total
antioxidant capacity (FRAP - 474 mg AEAC/100 g dwt), radical
scavenging capacity (DPPH - 4339.4 mh/100 g) and total
flavonoids (112 mg of catechin equivalents/100 g) compared to
fresh grapes (430, 3922 and 91 mg/100 g dwt of FRAP, DPPH and
total flavonoids respectively). Residues of Imidacloprid (Confidor
200 SL) on grapes was evaluated following spray applications (4
times) at recommended dose, revealed that crop was free of any
Imidacloprid residue at harvestng, 105 days after treatment.
Statistical model was developed to predict powdery mildew
incidence in grapes The prediction power of empirical model was
worked out to be 68.3%. Further, optimized model developed using
maximum temperature, relative humidity and rainfall predicted
powdery mildew incidence reasonably well (R
2
=0.60) and
possessed minimum average prediction errors. Statistical models
were developed to optimize the role of weather factors and
simultaneously to predict anthracnose incidence in grapes
cv. Anab-E-Shahi. The optimized model showed that morning and
evening relative humidity could predict the incidence to 77.5%.
Papaya
An advanced generation hybrid has been developed at IIHR,
Bangalore, which has medium-sized fruits (800 g), with deep pink
coloured pulp, high TSS (1314 Brix) and good keeping quality.
For quick detection of virus in plants and seedlings, molecular
diagnostic assay based on RT-PCR was developed using coat protein
gene specific primers. Under AICRP on Tropical Fruits at
Coimbatore, genotypes G 22, G 27, and G 15 were found early and
An advance generation hybrid of papaya was developed
Papaya genotypes, G 22, G 27 and G 15, were found
early and tolerant to cold
tolerant to cold. Fruits from these genotypes were harvested and
seeds collected to raise F
3
generation. Survey at Coimbatore revealed
2040% incidence of papaya ring spot virus (PRSV).
Sapota
Eleven accessions of sapota were characterized at IIHR,
Bangalore, using standard descriptors. The average fruit weight
ranged from 26.44 g in Badam to 130.42 g in DHS 2. Thirty-two
germplasm collections were studied for their susceptibility to chiku
moth (Nephopteryx eugraphella), bud-borer (Anarsia achrasella),
leaf miner (Acrocercops gemoniella) and ash weevil (Myllocerus
sp.). Leaf nutrient guides for sapota have been developed for
economic and balanced nutrient management. The nutrient guides
for micronutrients have also been developed for sapota farmers.
Potash has been identified as the most limiting nutrient in
orchards surveyed in Karnataka.
Eleven accessions of sapota were characterized with
standard descriptors.
Thirty-two germplasm accessions of sapota were studied
Under AICRP on Tropical Fruits, high-yielding clone
DHS 1(2/1) identified earlier continued to show its superiority at
Arabhavi. Application of 5 kg vermicompost with 200 g N, 40 g
P
2
O
5
and 150 g K
2
O/plant/year in Kalipatti recorded higher yield.
The treatment carbendazim (0.1%) effectively controlled leaf spot
disease of sapota.
Guava
CISH-G4, a selection from population of Apple Colour guava,
DARE/ICAR ANNUAL REPORT 20062007
42
was found promising, at CISH, Lucknow. It was released as Shweta
for commercial cultivation. This has globose, medium-sized fruits,
creamy white exocarp with red blush, snow white pulp, high TSS
(12.513.2%) content and vitamin C (300 mg/100 g EP) with good
keeping quality.
55 cultivars of litchi at NRC for Litchi, Muzaffapur. Litchi Shahi
proved to be more robust compared to other cultivars.Allied species
of litchi, longan (Dimocarpus longan) came to fruiting three
years after planting. The keeping quality of longan fruit was very
good and taste was sweeter than litchi with small seeds.
Major insect pests of litchi were identified. Trichogramma @
50,000/ha with Ninbicidine (0.5%) or cypermethrin @ (0.005%)
with Nimbicidine 0.5% sprays proved superior in minimizing the
damage by fruit-borer when sprayed at fruit setting (lentil-sized)
and at colour development stages.
One thousand four hundred litchi layers were supplied to litchi
growers during 2006. Qualitative characteristics of Shahi and
China litchi were analysed for wine making purpose.
Under AICRP on Sub-Tropical Fruits, double hedgerow system
of planting gave highest yield at Pantnagar, while square system at
Mohanpur. Shoots pruning up to 50 cm at the time of harvesting
of fruits, followed by removal of new flush in NovemberDecember,
2006, provided maximum yield of quality fruits at Pantnagar.
Leaf roller incidence reached its peak in October (4243%) and
caused 51.5% fruit damage 67 days after fruit setting at Mohanpur.
Incidence of leaf roller was low in March, 2006 and gradually
increased maximum level in July at Pantnagar.
Mango Karpuria, Hathijhool, Audhia Maldah, Police, Maldah
(Surajgarha) and Maldah (Dholikoth) showed only one per cent
of malformation. Critical temperature (19.4535.61C) and
relative humidity (52.9896%) with 9.01 h/day sunshine have been
found to favour the maximum disease development of powdery
mildew in Himsagar at Mohanpur. At Sangareddy, powdery mildew
was observed during fourth week of January, 2006 on susceptible
Ratna and Swarnajehangir with PDI of 6180 when mean
minimum temperature and mean maximum temperature were
10.71C and 28.8C.
The incidence of powdery mildew was first noticed on second
flush in first week of January at Vengurle. It was found to be
positively correlated to minimum temperature and panicle age. At
Paria, maximum disease (86%) was observed in Alphonso at mean
maximum temperature of 22.99C, minimum of 14.17C, RH of
68.64%, sunshine hour of 9.6 hours/day and vapour evaporation at
5.51 litre/day with clean sky position.
Passion fruit
Irrigation and fertilizer schedules for passion fruit revealed that
irrigation at 75% of evaporation replenishment recorded higher
yield as compared to irrigation at 50% of evaporation
replenishment and basin irrigation. Fertigation with 75 and 100%
of recommended dose of fertilizer recorded higher yield as
compared to soil application of fertilizer. This indicates a saving of
25% of water and fertilizer.
Under AICRP on Sub-Tropical Fruits, six germplasm accessions
were added to the germplasm collection. Lucknow-49 followed by
Allahabad Safeda and Lucknow-46-2 gave high yield at
Sangareddy. Guava Allahabad Safeda excelled under Sabour
conditions. The evaluation of germplasm at Bangalore revealed
that fruit weight was maximum (246.5 g) in Dhareedar followed
by Local Pink (244.2 g). The quality parameter revealed that TSS
Guava Shweta
Six accessions of guava were added
Three accessions of litchi were added
Twelve fruit species were collectged from Kerala
In Jammu, 12 collections were evaluated
Seven genotypes of aonla were added
Three genotypes of date palm were added
Aonla Selection 1 was identified
F 6/a, a new mateera, was ready for release
BS 1, a genotype of ber, was identified
Storage life of pomegranates of Bhagwa could be
extended for 3 weeks
ranged from 9.0 Brix in Aneuploid to 21.09 Brix in Local Pink.
The acidity was low (0.524%) in Thailand 2, followed by Thailand
1(0.613%). The vitaminC was more (298.2 mg/100 g pulp) in
Dhareedar. In planting system-cum-high-density planting,
maximum yield was recorded in double hedgerow system of
planting and minimum in square system.
Litchi
Three accessions were added to the gene bank making a total of
IMPROVEMENT AND MANAGEMENT OF HORTICULTURAL CROPS
43
Jackfruit
Jackfruit grafts of selected plants for raising clonal stocks at
Kannara and those of Palur 1 from Periyakulam centre are
maintained at Vellanikkara under AICRP on Tropical Fruits. At
Mohanpur, four new germplasm have been identified. Twelve elite
jackfruit clones were collected at Kovvur.
Underutilized fruits
In under-utilized fruits, three species of Pandanus, one each of
Baccaurea ramiflora, Spondias cytherea, Dillinia indica,
Averrhoa bilimbi, Malpigia glabra, Baccaurea sapida, Cordia
oblique, Artocarpus spp. and Garcinia spp. were added from
Andaman island. Another 12 fruit species found in Kerala forests
were also collected: Antidesma ghaesmbilla (Black current),
Salacia chinensis, S. beddomei, Flacourtia Montana, Syzygium
zeylanicum, Elaecarpus serratus, Chrysophyllum cainito
(Star apple), Flacourti (lovi-lovi), Averrhoa bilimbi (Bilimbli),
A. carambola (sour type), (Carambola), Psidium guineense
(Guva sp.), Aporusa lindleyana and Psidium littorale. In jamun,
12 collections were observed for their variable reaction to leaf
miner, Acrocercops sp., fruit weevil, Balaninus c- album, bark
eating cater pillar, Indarbela sp. and ash weevil, Mylloceros sp.
Fifteen pummelo germplasm collections were evaluated for
resistance to citrus leaf miner and Phyllocnistis citrella.
Temperate fruits
At CITH, Srinagar, medium high-density plantation with 625
budded plants of almond/ha at 4 m 4 m distance was compared
with 278 plants/ha planted traditionally at 6 m 6 m distance.
The budded plants commenced into bearing only after 3 years in
comparison to 7 years of seedling trees. Consequently, the
productivity has been raised from existing 0.86 tonne/ha to more
than 3 tonnes/ha from 6 years old plants. This showed the
possibility of increasing the productivity manifold with use of
varieties like Waris, Shalimar, Makhdoom and Non-Pareil.
Arid zone fruits
Seven genotypes of aonla and three of date palm were added to
national repository. Large collections of bael, jamun, karonda,
tamarind, mahua, chironji and wood apple were also made at
CHES, Vejalpur, Godhra. However, due to sub zero temperatures,
which prevailed continuously for 34 days during January 2006,
all germplasm of ber (except Tikadi and Snaur), aonla,
pomegranate and lasora were severely affected with frost injury
while date palm and khejri germplasm were not affected.
A selection of aonla (Aonla Selection 1) was identified, which is
early-maturing and high-yielding due to more number of female
flowers under rainfed conditions of semi-arid region. A new variety
of mateera (F6/a), which is free from cracking, good in TSS (9.5
11.2 Brix), low in seed content, red in pulp is ready to be released.
For tissue culture plant, a three step hardening process
involving primary in acclimatization hood made of plastic tray
covered with polycarbonate sheet with ventilation devices,
subsequent transfer to evaporative cool chamber and thereafter,
Medium high-density plantation in almond
Almond bearing in medium high-density plantation
acclimatization of plantlets either in shade house or low-cost
polyhouse equipped with intermittent fogging device has been
found effective in surviving of plants.
The fertigation practices has been standardized in Kinnow
mandarin and results revealed that 70 and 40% of recommended
Shalimar Non-pareil
DARE/ICAR ANNUAL REPORT 20062007
44
dose of nitrogen and phosphorus during FebruaryJune, 20, 50
and 40% of recommended dose of nitrogen, phosphorus and potash
during JulySeptember and remaining 10% of N and P and 60% of
potash during OctoberDecember may be applied through micro-
irrigation (drip and micro-sprinkler) for optimum productivity
with maximum water and nutrient-use efficiency.
mildew. The genotype has been registered as a promising line of
ber by NBPGR, New Delhi.
In custard-apple (Annona squamosa), additional pollination
gave best fruit setting, size and shape in both dry and wet years
without significantly affecting fruit quality. Under integrated
nutrient management programme, 50% of recommended doses of
NPK and S along with biofertilizers and recommended dose of FYM
proves to be best combination for aonla, ber, date palm and
pomegranate at different centers.
Application of neem oil 60 EC (A) @ 3% and neem oil 60 EC
@30% effectively reduced powdery mildew of ber and Trichoderma
viride 10 g/kg of soil reduced root rot incidence in different fruit
crops.
Post-harvest management
Mango fruit peels were found to possess very high antioxidant
activity. The antioxidants can be extracted from peels as valuable
byproducts from waste. Hot water treatment of fruits of mango
Chausa, Amrapali amd Mallika at 48C 1C for 1 hour
controlled all stages of mango fruit fly (Bactrocera zonata).
Individual shrink-wrapping of mature green fruts of Alphonso and
Banganapalli with semi-permeable polymeric films extended their
green storage life to 5 weeks at 8C without any chilling injury
symptoms. Alphonso mangoes packed in bulk by inner lining of
entire 4 kg CFB box with micro-perforated semi-permeable films
could be stored for 1 month at 8C in unripe hard green condition
without any chilling injury symptoms.
The storage life of pomegranate fruits (Bhagwa) could be extended
to 3 weeks by bulk MAP and 1 month by individual shrink wrapping at
ambient temperature. At 8C, storage life could be extended to 3
months by these packing methods with a weight loss of less than 1% as
compared to 20% weight loss in non-wrapped fruits.
VEGETABLE CROPS
Crop impovement
Chilli Kashi Anmol a determinate variety, suitable for production
of green fruits, has been developed. Its fruits are dark green, 56 cm
long and 2 cm girth, pungent (0.6% capsaicin) with smooth surface,
early fruiting, first picking 55 days after transplanting, with an
average yield of 250 q/ha (green fruits). Kashi Early is a hybrid with
early fruiting suitable for production of green fruits. Its fruits are
dark green, 67 cm long, 2.3 cm girth with smooth surface; first
picking starts 45 days after transplanting. Its average yield is 275
tonnes/ha (green fruits). It is recommended for cultivation in Bihar,
Uttar Pradesh, Uttaranchal, Jharkhand, Delhi and Punjab. Kashi
Vishwanath is a cms based hybrid suitable for dry fruit production.
Its fruits are 1011 cm long, 22.3 cm girth with wrinkled surface,
light yellow-green, average yield of 200 q/ha (red ripe fruits). Its is
Veneer grafting in khirnee (Manilkara
hexandra)
Aonla cider
Under microirrigation system, maximum root volumes were
estimated in 2040 cm deep soil layers in pomegranate, kinnow
and ber trees. During AprilJune 2845% more moisture was
conserved under black polythene mulch in aonla. In arid
conditions, FYM mulch (140 tonnes/ha) increased the water-use
efficiency, moderate the extreme soil temperatures and increased
fruit yield in brinjal and kachri crops. The use of vermicompost in
sandy soils has better influence on soil moisture retention in root
zone for a longer period, which also helps to make the nutrients
available for plants.
Pomegranate and aonla fruits infested by post-harvest
pathogens were investigated for presence of mycotoxins with special
reference to Aflatoxins. Aonla, NA-6, NA-7, Chakaiya and Krishna
were observed with infection due to Aspergillus spp. and it was
maximum (33.2%) in Krishna, followed by NA 6 (17.8%). Four
pathogenic isolates of Aspergillus spp. were tested for mycotoxins
and aflatoxins, viz. B
1
, B
2,
G
1
and G
2.
These were produced in growth
media and fruits as well. Detection of aflatoxins in pomegranate
arils by agar plugs method showed positive response with different
compounds when the samples were loaded on silica gel (TLC).
Aflatoxin B
2
was secreted by all isolates in growth media and
infected arils. The fungicidal seed dressing with ridomil Mz @ 2.5
g/kg seed was adjudged as best treatment which provide maximum
transplant yield with least mortality due to post-emergence
damping off disease.
Under AICRP on Arid Zone Fruits, a genotype of ber BS-1 has
been identified. It is tolerant to fruit fly and resistant to powdery
IMPROVEMENT AND MANAGEMENT OF HORTICULTURAL CROPS
45
recommended for cultivation in Bihar, Delhi, Haryana, Andhra
Pradesh, Karnataka and Tamil Nadu.
Okra IIVR 11 is a new variety. Its plants grow 110130 cm
high, flowering starts 3034 days after sowing. It is suitable for
cultivation during rainy as well as summer season. Fruits are of
five ridges, green and 1315 cm long. This is resistant to YVMV
with an average yield of 150170 q/ha. Its is recommended for
cultivation in Chhattisgarh, Orissa, Andhra Pradesh, Rajasthan,
Gujarat, Haryana and Delhi.
Cauliflower Kashi Kunwari is an early-maturing variety. Its curd
is semi-dome type, white compact; fine texture, curd weight 300
450 g with an average yield of 300350 q/ha. It is suitable for
cultivation in Punjab, Uttar Pradesh, Bihar and Jharkhand.
Radish Kashi Hans is suitable for September to February
planting and harvesting can be done 4045 days after sowing. Its
roots are straight, tapering, 3035 cm long, 3.54.2 cm diameter;
with an yield potential of 430450 q/ha. It is released for
cultivation in Uttar Pradesh, Punjab, Bihar and Jharkhand. Cowpea
Kashi Gauri is bushy, dwarf, photo-insensitive and early variety
suitable for sowing in both spring-summer and rainy seasons. It
flowers in 3538 days and pods becomes ready for harvesting 45-
48 days after sowing. Pods are 2530 cm long, green, tender, fleshy,
less fibrous and free from parchment layer. Resistant to golden
mosaic virus and Pseudocercospora cruenta. It produces 100125
q/ha green pods.
Genetic transformation: Genetic transformation: Genetic transformation: Genetic transformation: Genetic transformation: Transformation was performed
using Agrobacterium tumefaciens in tomato H 86 using Cry 1Ac
gene from T-DNA of binary vector plasmid pBinAR. More than 85
putative transgenic plants were regenerated and are being
maintained under laboratory conditions. The putative transgenic
plants were tested for confirmation of inserted gene through PCR
analysis. The inserted gene was detected by npt II gene and Cry 1
Ac gene specific primer which shows 700 bp for npt II and 900 bp
for Cry 1 Ac gene. After PCR analysis, positive plants were subjected
to southern blot hybridization. The PCR positive plants were also
tested for strip test (strip that contain Anti Cry 1 AC antibody) and
ELISA test. PCR and southern positive plants are being used for
insect bioassay and segregation analysis.
T TT TTransformation: ransformation: ransformation: ransformation: ransformation: Brinjal genotypes Punjab, Sadabahar, Pant
Rituraj, VR Baigan 1 (IVBR 1), VR Baigan 3 (IVBR 3), VR Baigan 9
(IVBL 9) and VR Baigan 14 (BRSPS 14), were used for regeneration
and transformation. Transformation was performed using
Agrobacterium tumefaciens in VR Baigan 9 (IVBL 9) using Cry 1
Ac gene from T-DNA of binary vector plasmid pBinAR. More than
80 putative transgenic plants were regenerated. The gene
integration was detected by PCR analysis. The amplification of 0.7
kb band for npt II and 0.9 kb for Cry 1 Ac shows the presence of
inserted gene. The transformants were further analyzed by southern
blot hybridization. Southern analysis revealed 1 kb band with
single blot hybridization in most of the transformants. The
presence of Cry 1 Ac toxin protein in plant was confirmed strip
A number of new vegetable varieties were released
More than 85 putative transgenic plants were regenerated
Brinjal genotypes were used for regeneration and
transformation
F
1
hybrids of chilli were analysed for purity
A total of 200 primers were used for screening
polymorphism
A technique to estimate antioxidant activity in tomato has
been standardized
Thirty-two isolates of A. flavus were studied
Chilli Kashi Anmol
Chilli Kashi Vishwanath
DARE/ICAR ANNUAL REPORT 20062007
46
Cauliflower Kunwari Radish Kashi Hans
Cowpea Kashi Gauri
coated with anti cry 1 Ac antibodies. Insect bioassay was also done
using neonate larvae (Leucinodes orbonalis) brinjal shoot-and
fruit-borer with PCR and southern blot analysis confirmed
transformants. Segregation analysis was done with only PCR and
Southern positive plants and most of plant showed 3 : 1 ratio.
Resistance sources to Pep-LCV Resistance sources to Pep-LCV Resistance sources to Pep-LCV Resistance sources to Pep-LCV Resistance sources to Pep-LCV: :: :: From field screened 307
genotypes against pepper leaf curl virus (PepLCV) during previous
season, selfed progenies of eight symptom-less and highly resistant
lines were challenged by viruliferous white fly under glasshouse
conditions. Of them, GKC 29, BS 35 and EC 497636, showed no
symptoms. Using scion and rootstalk of susceptible genotype (Pusa
Jwala), these three putative symptomless genotypes were further
challenged by grafting and alternate grafting. The resistant
reactions of GKC 29, BS 35, EC 497636 were confirmed because
even after 50 days of successful grafting/alternate grafting, no viral
symptom appeared on all grafted plants of Pusa Jawala in all three
genotypes. When subjected to PCR amplification with degenerate
primers designed to detect begamovirus like PepLCV, three
symptom-less genotypes did not show any amplification, suggesting
that resistant reaction in three identified resistant sources was
because of absence/non-replication of viral genome and these lines
are not symptom-less carrier.
Genetic purity testing: Genetic purity testing: Genetic purity testing: Genetic purity testing: Genetic purity testing: Two commercial F
1
hybrids of chilli,
CCH 2 (A
1
Pusa Jwala) and CCH 3 (KA 2 R Line), and their
corresponding parents were analyzed for purity testing. A total of
200 primers (10 mer) were used for screening polymorphism
between two pairs of parents. Of these primers, 145 produced clear
amplification products. On an average each primer amplified three
scorable bands. The primers that generated bands specific to male
parent were repeated at least 23 times. These primers giving the
same pattern in two or more replicates were chosen for further use.
The 4 primers were found useful in determining seed purity. For
hybrid CCH 2, 2 male specific bands were found with primers OPZ 6
and OPY 20 and among these, OPZ 6 was found most suitable for
hybrid purity testing with band size of 700 bp. For hybrid CCH 3, 2
male specific bands with primers (OPS 1 and OPQ 18) were found
amongst which OPS 1 was found most suitable for hybrid purity
testing with band size of 400 bp.
Crop production
Integrated nutrient management: Integrated nutrient management: Integrated nutrient management: Integrated nutrient management: Integrated nutrient management: Ten organic nutrient
sources, i.e. FYM @ 20 tonnes/ha, sewage sludge @ 20 tonnes/ha,
vermicompost @ 10 tonnes/ha, neem cake @5 q/ha, NPK @150 :
60 : 80 kg/ha, FYM @ 20 tonnes/ha +Azotobactor, sewage sludge @
20 toones/ha + Azotobactor, vermicompost @ 10 tonnes/ha +
Azotobactor, neem cake @ 5 q/ha + Azotobactor, NPK @150 : 60 : 80
kg/ha + Azotobactor were given to tomato Sartaj.The observations
reveal that application of vermicompost @ 10 tonnes/ha +
Azotobactor gave significantly higher yield (962.5 q/ha), plant height
(118.6), fruit size (37.8 cm
2
) compared to other treatments. The
minimum values were noted under sewage sludge treated plots.
Symptomless GKC 29 scion grafted on susceptible Pusa Jwala
IMPROVEMENT AND MANAGEMENT OF HORTICULTURAL CROPS
47
Antioxidant activity (AOA) in tomato: Antioxidant activity (AOA) in tomato: Antioxidant activity (AOA) in tomato: Antioxidant activity (AOA) in tomato: Antioxidant activity (AOA) in tomato: A technique has
been standardized for estimation of antioxidant activity in tomato
fruits to assess variability. Total antioxidant activity of sample ex-
tracts was analyzed using 2, 2-diphenyl-1-picryl-hydrazyl (DPPH)
by recording absorbance at 515 nm. The standard curve was pre-
pared for the reaction of TROLOX with DPPH and data was con-
verted to activity in terms of moles trolox equivalents/100 g sam-
ple. Significant differences (p>0.05) were recorded for antioxidant
activity among 15 cultivars which ranged from 2.19 to 5.79
moles trolox equivalents/g with an overall mean for all the
entries of 3.854 moles trolox equivalents/g.
Effect of bioagents: Effect of bioagents: Effect of bioagents: Effect of bioagents: Effect of bioagents: Thirty-two isolates of A. flavus were
studied for radial growth inhibition against Trichoderma viride,
T. harzianum and T. koenigii. Mean radial growth inhibition of
30 plates of A. flavus by three biocontrol agents in dual culture
showed general inhibition of mycelial diameter above 60% in
almost all test combinations. Relative inhibition efficacy varied
among test bioagents. Maximum inhibition of A. flavus isolates
with bioagent T. viride which not only restrict the growth of
meeting point but also fully overlap growth of test organism.
Use of bait: Use of bait: Use of bait: Use of bait: Use of bait: During peak infestation period, molasses (10%) +
carbaryl (0.1%) cover spray recorded significantly lowest (20.36%)
fruit infestation being at par with spot spray of molasses (10%) +
carbaryl (0.1%) manifesting 27.4% fruit damage while in control
plot 55.28% fruits were damaged by fruit fly. Mean cumulative fruit
damage over all the pickings indicated the superiority of molasses
bait in either of the treatments. Molasses bait spray in spots
recorded minimum (18.51%) followed non-significantly by
molasses + insecticide cover spray (19.94%). The level of fruit
damage in most usually applied insecticide cover spray was 26.67%.
The control plot recorded 37.54% fruit damage. Maximum healthy
fruit yield was recorded in molasses bait spray applied in spot.
Effect of seed treatment: Effect of seed treatment: Effect of seed treatment: Effect of seed treatment: Effect of seed treatment: The mean seedling length
(25.26 cm) and vigour index (2,357.82) were maximum in
thiamethoxam treated seeds days after sowing. Seedling vigour in-
dex in carbosulfan treatment was adversely affected due to low
germination. Thiamethoxam was most ideal treatment with
maximum plant dry weight (70.01 g) 50 days after sowing. In
jassid population thiamethoxam also was significantly superior to
all treatments harbouring least number of jassids (9.46/plant)
compared to control (27.59 jassids/plant). The yield in this
treatment was also highest (126.82 q/ha) followed by imidacloprid
(112.01 q/ha).
Tomato varieties at farmers fields
Tomato Kashi Amrit, Kashi Anupam and Kashi Vishesh
developed by IIVR Varanasi, were demonstrated at farmers fields of
Varanasi, Chandauli, Sonebhadra and Mirzapur districts in Uttar
Pradesh. On an average, all varieties gave 520.83 q/ha compared
to 365.25 q/ha (control). All these varieties are spreading at
Staked tomato grown under IPNM
Yield of tomato Kashi Vishesh at farmers fields
Performance of Kashi Vishesh at farmers fields
DARE/ICAR ANNUAL REPORT 20062007
48
farmers fields very rapidly due to their better productivity. Tomato
Kashi Vishesh has proven a boon for mid-season tomato growers.
In rainfed condition, in Sonebhadra district farmers taking only
wheat as a major crop now are growing tomato as a major cash
crop. The farmers express that flesh and hardness of all the varieties
are like hybrids, which help them for distant marketing and
improving their socio-economic status.
MUSHROOM
The National Mushroom Repository has been enriched by adding
312 mushroom cultures. Of which, a few are new records for India.
Genetic improvement studies of temperate and tropical mushrooms
Two parental lines, i.e. JW/96 (a good combiner of yield) and E/
79-42 with combined resistance to late blight and potato cyst
nematode have been registered as elite germplasm by ICAR/NBPGR
germplasm. Potato Kufri Surya, Kufri Arun and Kufri Chipsona 3
were released for commercial cultivation. Hybrid MP/97-644
combining high dry-matter and low suger contents, acceptable chip
colour having resistance to late blight has been identified. Nine
transgenic lines expressing AmA1 gene had significantly higher
(2049%) total soluble protein content. The osmotin gene cloned
from wild species, Solanum chacoense, was introduced into late
blight susceptible cultivars. Transgenicity of these lines was
confirmed by molecular analysis. Reduction in lesion size due to
Of the 312 mushroom cultures, a few of them are new
ones.
Fifty-three strains of mushrooms were evaluated
Spent mushroom substrate and coir pith gave highest
mushroom yield
Polyprophylene bags were best for containers
A semi-automatic compost turner has been fabricated
revealed the identity of several single spore selections in Volvariella
volvacea, Agaricus bisporus and hybrids in Pleurotus sajor-caju.
Fifty three hybrid strains of Pleurotus sajor-caju were evaluated
on wheat straw. Of them, 10 strains gave more than 68-85% BE. In
paddy straw mushroom, 42 single spore isolates were compared for
their different growth characters. Molecular characterization of
various Mycogone cultures collected from different mushroom
farms revealed no genetic variability, whereas Trichoderma isolates
collected from various locations were identified as Trichoderma
asperellum, T. harzianum T. longibrachiatum and T. virens.
The spent mushroom substrate and coir pith gave highest
mushroom yield. Polypropylene bags proved to be best containers for
cultivation of Flammulina velutipes. Supplementation of 20% wheat
bran proved better for increasing productivity of shiitake mushroom.
Another medicinal mushroom (Schizophylum commune) has been
also successfully cultivated on sawdust. Among different cultures of
Ganoderma lucidum, Thai culture gave highest yield followed by
Korean OE 53. The cultivation of button, oyster and paddy straw
mushrooms in low-cost bamboo huts with good yields were
demonstrated to framers. The design of semi-automatic compost
turner of 5 tonnes/hour capacity is being fabricated.
TUBER CROPS
Potato
Germplasm collection was raised to 2,850 accessions by
augmenting cultivated and wild species obtained from 30 countries.
Germplasm collection of potato was enriched to 2,850
accessions
JW/96 and E/79-42 were registerd as elite germplasm
Kufri Surya, Kufri Arun and Kufri Chipsona 3 were released
Nine transgenic lines of Kufri Badshah had better colour
in their chips
Russet scab has been important disease of potato
A highly sensitive PCR technique to detect potato leaf curl
was developed
Eight meiotic cultivars showed resistance to late blight
Potato salties, potato sweet nuggets and potato sewia
new value-added productswere developed
Sree Athulya and Sree Apoorva, new cassava varieties,
were recommended
Two orange-fleshed clones of sweet potato were identified
late blight pathogen was observed in all these lines. Work on
production of transgenic lines carrying resistance to bacterial wilt
and potato tuber moth had yielded promising results. Fourteen
transgenic lines of potato Kufri Badshah harbouring tobacco
invertase inhibitor gene, Nt-Inhh showed substantial improvement
in chip colour compared to non-
transgenic control tubers. Putative
subunits of potato RNase P (an
endonuclease) were identified,
cloned and sequenced.
At Patna, rice equivalent yield and
economic returns were highest in
potatoonionrice followed by
potatomaizerice crop sequence,
Leaf, flower, sprout, DNA fingerprints
and tubers of MP/97-644 a promising
hybrid for hills